β amyloid immune complexes  (Millipore)


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    Structured Review

    Millipore β amyloid immune complexes
    ELISA determination of <t>β-amyloid</t> immune complexes (Aβ-IgG) and free Aβ-autoantibodies in human serum. (A) Sandwich ELISA to determine Aβ-IgG immune complexes: mAb 6E10, recognizing Aβ(3–8) epitope, is first coated on the ELISA plates. After blocking with BSA, human serum containing β-amyloid immune complexes is added. The detection is performed with a labeled anti-IgG antibody. (B) Indirect ELISA to determine free Aβ-autoantibodies: Biotin-G 5 -Aβ(12–40) is immobilized on streptavidin coated ELISA plates. After blocking with BSA, the addition of serum containing free Aβ-autoantibodies leads to their binding to Aβ(12–40). The detection is performed with a labeled anti-IgG antibody.
    β Amyloid Immune Complexes, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β amyloid immune complexes/product/Millipore
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    β amyloid immune complexes - by Bioz Stars, 2020-08
    85/100 stars

    Images

    1) Product Images from "Antigen-Bound and Free ?-Amyloid Autoantibodies in Serum of Healthy Adults"

    Article Title: Antigen-Bound and Free ?-Amyloid Autoantibodies in Serum of Healthy Adults

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0044516

    ELISA determination of β-amyloid immune complexes (Aβ-IgG) and free Aβ-autoantibodies in human serum. (A) Sandwich ELISA to determine Aβ-IgG immune complexes: mAb 6E10, recognizing Aβ(3–8) epitope, is first coated on the ELISA plates. After blocking with BSA, human serum containing β-amyloid immune complexes is added. The detection is performed with a labeled anti-IgG antibody. (B) Indirect ELISA to determine free Aβ-autoantibodies: Biotin-G 5 -Aβ(12–40) is immobilized on streptavidin coated ELISA plates. After blocking with BSA, the addition of serum containing free Aβ-autoantibodies leads to their binding to Aβ(12–40). The detection is performed with a labeled anti-IgG antibody.
    Figure Legend Snippet: ELISA determination of β-amyloid immune complexes (Aβ-IgG) and free Aβ-autoantibodies in human serum. (A) Sandwich ELISA to determine Aβ-IgG immune complexes: mAb 6E10, recognizing Aβ(3–8) epitope, is first coated on the ELISA plates. After blocking with BSA, human serum containing β-amyloid immune complexes is added. The detection is performed with a labeled anti-IgG antibody. (B) Indirect ELISA to determine free Aβ-autoantibodies: Biotin-G 5 -Aβ(12–40) is immobilized on streptavidin coated ELISA plates. After blocking with BSA, the addition of serum containing free Aβ-autoantibodies leads to their binding to Aβ(12–40). The detection is performed with a labeled anti-IgG antibody.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Sandwich ELISA, Blocking Assay, Labeling, Indirect ELISA, Binding Assay

    2) Product Images from "Antigen-Bound and Free ?-Amyloid Autoantibodies in Serum of Healthy Adults"

    Article Title: Antigen-Bound and Free ?-Amyloid Autoantibodies in Serum of Healthy Adults

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0044516

    ELISA determination of β-amyloid immune complexes (Aβ-IgG) and free Aβ-autoantibodies in human serum. (A) Sandwich ELISA to determine Aβ-IgG immune complexes: mAb 6E10, recognizing Aβ(3–8) epitope, is first coated on the ELISA plates. After blocking with BSA, human serum containing β-amyloid immune complexes is added. The detection is performed with a labeled anti-IgG antibody. (B) Indirect ELISA to determine free Aβ-autoantibodies: Biotin-G 5 -Aβ(12–40) is immobilized on streptavidin coated ELISA plates. After blocking with BSA, the addition of serum containing free Aβ-autoantibodies leads to their binding to Aβ(12–40). The detection is performed with a labeled anti-IgG antibody.
    Figure Legend Snippet: ELISA determination of β-amyloid immune complexes (Aβ-IgG) and free Aβ-autoantibodies in human serum. (A) Sandwich ELISA to determine Aβ-IgG immune complexes: mAb 6E10, recognizing Aβ(3–8) epitope, is first coated on the ELISA plates. After blocking with BSA, human serum containing β-amyloid immune complexes is added. The detection is performed with a labeled anti-IgG antibody. (B) Indirect ELISA to determine free Aβ-autoantibodies: Biotin-G 5 -Aβ(12–40) is immobilized on streptavidin coated ELISA plates. After blocking with BSA, the addition of serum containing free Aβ-autoantibodies leads to their binding to Aβ(12–40). The detection is performed with a labeled anti-IgG antibody.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Sandwich ELISA, Blocking Assay, Labeling, Indirect ELISA, Binding Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Antigen-Bound and Free ?-Amyloid Autoantibodies in Serum of Healthy Adults
    Article Snippet: .. Using the optimized ELISA protocol described in Materials and Methods, we investigated the presence of β-amyloid immune complexes in two IgG preparations: (1) human serum IgG from Calbiochem, commercialized for research purposes only and (2) intravenous immune globuline (IVIgG; Gamunex® 10%) from Talecris Biotherapeutics (Frankfurt am Main, Germany), in use for treatment of different infectious, inflammatory and autoimmune disorders. β-amyloid immune complexes were detected in both preparations, slightly higher levels being observed in the product from Calbiochem ( , supporting information). ..

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    Millipore β amyloid immune complexes
    ELISA determination of <t>β-amyloid</t> immune complexes (Aβ-IgG) and free Aβ-autoantibodies in human serum. (A) Sandwich ELISA to determine Aβ-IgG immune complexes: mAb 6E10, recognizing Aβ(3–8) epitope, is first coated on the ELISA plates. After blocking with BSA, human serum containing β-amyloid immune complexes is added. The detection is performed with a labeled anti-IgG antibody. (B) Indirect ELISA to determine free Aβ-autoantibodies: Biotin-G 5 -Aβ(12–40) is immobilized on streptavidin coated ELISA plates. After blocking with BSA, the addition of serum containing free Aβ-autoantibodies leads to their binding to Aβ(12–40). The detection is performed with a labeled anti-IgG antibody.
    β Amyloid Immune Complexes, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β amyloid immune complexes/product/Millipore
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    β amyloid immune complexes - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    ELISA determination of β-amyloid immune complexes (Aβ-IgG) and free Aβ-autoantibodies in human serum. (A) Sandwich ELISA to determine Aβ-IgG immune complexes: mAb 6E10, recognizing Aβ(3–8) epitope, is first coated on the ELISA plates. After blocking with BSA, human serum containing β-amyloid immune complexes is added. The detection is performed with a labeled anti-IgG antibody. (B) Indirect ELISA to determine free Aβ-autoantibodies: Biotin-G 5 -Aβ(12–40) is immobilized on streptavidin coated ELISA plates. After blocking with BSA, the addition of serum containing free Aβ-autoantibodies leads to their binding to Aβ(12–40). The detection is performed with a labeled anti-IgG antibody.

    Journal: PLoS ONE

    Article Title: Antigen-Bound and Free ?-Amyloid Autoantibodies in Serum of Healthy Adults

    doi: 10.1371/journal.pone.0044516

    Figure Lengend Snippet: ELISA determination of β-amyloid immune complexes (Aβ-IgG) and free Aβ-autoantibodies in human serum. (A) Sandwich ELISA to determine Aβ-IgG immune complexes: mAb 6E10, recognizing Aβ(3–8) epitope, is first coated on the ELISA plates. After blocking with BSA, human serum containing β-amyloid immune complexes is added. The detection is performed with a labeled anti-IgG antibody. (B) Indirect ELISA to determine free Aβ-autoantibodies: Biotin-G 5 -Aβ(12–40) is immobilized on streptavidin coated ELISA plates. After blocking with BSA, the addition of serum containing free Aβ-autoantibodies leads to their binding to Aβ(12–40). The detection is performed with a labeled anti-IgG antibody.

    Article Snippet: Using the optimized ELISA protocol described in Materials and Methods, we investigated the presence of β-amyloid immune complexes in two IgG preparations: (1) human serum IgG from Calbiochem, commercialized for research purposes only and (2) intravenous immune globuline (IVIgG; Gamunex® 10%) from Talecris Biotherapeutics (Frankfurt am Main, Germany), in use for treatment of different infectious, inflammatory and autoimmune disorders. β-amyloid immune complexes were detected in both preparations, slightly higher levels being observed in the product from Calbiochem ( , supporting information).

    Techniques: Enzyme-linked Immunosorbent Assay, Sandwich ELISA, Blocking Assay, Labeling, Indirect ELISA, Binding Assay