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    Name:
    β Actin D6A8 Rabbit mAb
    Description:
    Actin a ubiquitous eukaryotic protein is the major component of the cytoskeleton At least six isoforms are known in mammals Nonmuscle β and γ actin also known as cytoplasmic actin are predominantly expressed in nonmuscle cells controlling cell structure and motility 1 α cardiac and α skeletal actin are expressed in striated cardiac and skeletal muscles respectively two smooth muscle actins α and γ actin are found primarily in vascular smooth muscle and enteric smooth muscle respectively These actin isoforms regulate the contractile potential of muscle cells 1 Actin exists mainly as a fibrous polymer F actin In response to cytoskeletal reorganizing signals during processes such as cytokinesis endocytosis or stress cofilin promotes fragmentation and depolymerization of F actin resulting in an increase in the monomeric globular form G actin 2 The ARP2 3 complex stabilizes F actin fragments and promotes formation of new actin filaments 2 Research studies have shown that actin is hyperphosphorylated in primary breast tumors 3 Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle as shown in research studies 4 6 Actin cleavage by caspase 3 may accelerate ubiquitin proteasome dependent muscle proteolysis 6
    Catalog Number:
    8457
    Price:
    None
    Category:
    Primary Antibodies
    Source:
    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-actin protein.
    Reactivity:
    Human Mouse Rat Monkey D melanogaster Zebrafish
    Applications:
    Western Blot, Immunofluorescence, Flow Cytometry
    Buy from Supplier


    Structured Review

    Cell Signaling Technology Inc β actin
    Effects of Scrophularia buergeriana extract (SBE) on apoptotic protein expression levels in SH-SY5Y cells. (A) The expression of phosphorylated (p)-p38, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), cleaved caspase-3 and cleaved poly (adenosine diphosphate (ADP)-ribose) polymerase (PARP) was measured by western blot analysis. (B) The density of the protein bands was quantified and calculated using ImageJ software. p-p38 protein expression levels were normalized to those of p38, and other protein expression levels were normalized to those of <t>β-actin.</t> The data are expressed as the means ± SEM of independent experiments (n=3). * P
    Actin a ubiquitous eukaryotic protein is the major component of the cytoskeleton At least six isoforms are known in mammals Nonmuscle β and γ actin also known as cytoplasmic actin are predominantly expressed in nonmuscle cells controlling cell structure and motility 1 α cardiac and α skeletal actin are expressed in striated cardiac and skeletal muscles respectively two smooth muscle actins α and γ actin are found primarily in vascular smooth muscle and enteric smooth muscle respectively These actin isoforms regulate the contractile potential of muscle cells 1 Actin exists mainly as a fibrous polymer F actin In response to cytoskeletal reorganizing signals during processes such as cytokinesis endocytosis or stress cofilin promotes fragmentation and depolymerization of F actin resulting in an increase in the monomeric globular form G actin 2 The ARP2 3 complex stabilizes F actin fragments and promotes formation of new actin filaments 2 Research studies have shown that actin is hyperphosphorylated in primary breast tumors 3 Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle as shown in research studies 4 6 Actin cleavage by caspase 3 may accelerate ubiquitin proteasome dependent muscle proteolysis 6
    https://www.bioz.com/result/β actin/product/Cell Signaling Technology Inc
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    β actin - by Bioz Stars, 2021-03
    98/100 stars

    Images

    1) Product Images from "Neuroprotective effects of Scrophularia buergeriana extract against glutamate-induced toxicity in SH-SY5Y cells"

    Article Title: Neuroprotective effects of Scrophularia buergeriana extract against glutamate-induced toxicity in SH-SY5Y cells

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2019.4139

    Effects of Scrophularia buergeriana extract (SBE) on apoptotic protein expression levels in SH-SY5Y cells. (A) The expression of phosphorylated (p)-p38, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), cleaved caspase-3 and cleaved poly (adenosine diphosphate (ADP)-ribose) polymerase (PARP) was measured by western blot analysis. (B) The density of the protein bands was quantified and calculated using ImageJ software. p-p38 protein expression levels were normalized to those of p38, and other protein expression levels were normalized to those of β-actin. The data are expressed as the means ± SEM of independent experiments (n=3). * P
    Figure Legend Snippet: Effects of Scrophularia buergeriana extract (SBE) on apoptotic protein expression levels in SH-SY5Y cells. (A) The expression of phosphorylated (p)-p38, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), cleaved caspase-3 and cleaved poly (adenosine diphosphate (ADP)-ribose) polymerase (PARP) was measured by western blot analysis. (B) The density of the protein bands was quantified and calculated using ImageJ software. p-p38 protein expression levels were normalized to those of p38, and other protein expression levels were normalized to those of β-actin. The data are expressed as the means ± SEM of independent experiments (n=3). * P

    Techniques Used: Expressing, Western Blot, Software

    Effects of Scrophularia buergeriana extract (SBE) on antioxidant protein expression levels in SH-SY5Y cells. (A) The expression levels of superoxide dismutase (SOD)1, SOD2, and glutathione peroxidase-1 (GPx-1) were measured by western blot analysis. (B) The density of the protein bands was quantified and calculated using ImageJ software. Protein expression levels were normalized to those of β-actin. The data are expressed as the means ± SEM of independent experiments (n=3). * P
    Figure Legend Snippet: Effects of Scrophularia buergeriana extract (SBE) on antioxidant protein expression levels in SH-SY5Y cells. (A) The expression levels of superoxide dismutase (SOD)1, SOD2, and glutathione peroxidase-1 (GPx-1) were measured by western blot analysis. (B) The density of the protein bands was quantified and calculated using ImageJ software. Protein expression levels were normalized to those of β-actin. The data are expressed as the means ± SEM of independent experiments (n=3). * P

    Techniques Used: Expressing, Western Blot, Software

    2) Product Images from "The opposing roles of the mTOR signaling pathway in different phases of human umbilical cord blood‐derived CD34+ cell erythropoiesis, et al. The opposing roles of the mTOR signaling pathway in different phases of human umbilical cord blood‐derived CD34+ cell erythropoiesis"

    Article Title: The opposing roles of the mTOR signaling pathway in different phases of human umbilical cord blood‐derived CD34+ cell erythropoiesis, et al. The opposing roles of the mTOR signaling pathway in different phases of human umbilical cord blood‐derived CD34+ cell erythropoiesis

    Journal: Stem Cells (Dayton, Ohio)

    doi: 10.1002/stem.3268

    Rapamycin regulates autophagy by inhibiting the mTOR/p70S6K/S6 signaling pathway and activating autophagy genes. Cells were treated with rapamycin from day 11. Blank and DMSO were used as controls. A, Western blot analysis for mTOR signaling proteins (p‐p70S6K [T389], p70S6K p‐S6 [S235/236], and S6) in cells on days 14, 18, and 21. B, Western blot analysis for autophagy markers (LC3 and p62) in cells on days 14, 18, and 21. C, Expression of LC3 in erythroid progenitor cells and K562 cells as determined by western blotting. K562 cells were treated with different concentrations of rapamycin and harvested after 48 hours. D, Western blot analysis for autophagy‐related proteins (FIP200, ULK1, ATG7, ATG12, ATG5, BECLIN, ATG3, and ATG101) in cells on days 14, 18, and 21. E, Protein expression was quantified by densitometry and normalized to β‐actin expression. Data are the mean ± SD of technical triplicates from one of several independent experiments. * P
    Figure Legend Snippet: Rapamycin regulates autophagy by inhibiting the mTOR/p70S6K/S6 signaling pathway and activating autophagy genes. Cells were treated with rapamycin from day 11. Blank and DMSO were used as controls. A, Western blot analysis for mTOR signaling proteins (p‐p70S6K [T389], p70S6K p‐S6 [S235/236], and S6) in cells on days 14, 18, and 21. B, Western blot analysis for autophagy markers (LC3 and p62) in cells on days 14, 18, and 21. C, Expression of LC3 in erythroid progenitor cells and K562 cells as determined by western blotting. K562 cells were treated with different concentrations of rapamycin and harvested after 48 hours. D, Western blot analysis for autophagy‐related proteins (FIP200, ULK1, ATG7, ATG12, ATG5, BECLIN, ATG3, and ATG101) in cells on days 14, 18, and 21. E, Protein expression was quantified by densitometry and normalized to β‐actin expression. Data are the mean ± SD of technical triplicates from one of several independent experiments. * P

    Techniques Used: Western Blot, Expressing

    Rapamycin induces cell‐cycle arrest in erythroid precursor cells. A, Erythroid precursor cells were treated with or without rapamycin from day 8 or day 11 for 72 hours. Then, the cells were stained with propidium iodide and subjected to flow cytometry to analyze DNA content. The right panel shows the fractions (%) of cells in G0/G1, S, and G2/M phases. B, p27 mRNA expression in cells on day 11 and 14 treated with or without rapamycin from day 8 as analyzed by RT‐qPCR. Gene expression was normalized to β‐actin. C, Representative images of p27 protein expression in cells on days 11 and 14 treated with or without rapamycin from day 8 as determined by western blot analysis. Data are the mean ± SD of technical triplicates from one of several independent experiments. * P
    Figure Legend Snippet: Rapamycin induces cell‐cycle arrest in erythroid precursor cells. A, Erythroid precursor cells were treated with or without rapamycin from day 8 or day 11 for 72 hours. Then, the cells were stained with propidium iodide and subjected to flow cytometry to analyze DNA content. The right panel shows the fractions (%) of cells in G0/G1, S, and G2/M phases. B, p27 mRNA expression in cells on day 11 and 14 treated with or without rapamycin from day 8 as analyzed by RT‐qPCR. Gene expression was normalized to β‐actin. C, Representative images of p27 protein expression in cells on days 11 and 14 treated with or without rapamycin from day 8 as determined by western blot analysis. Data are the mean ± SD of technical triplicates from one of several independent experiments. * P

    Techniques Used: Staining, Flow Cytometry, Expressing, Quantitative RT-PCR, Western Blot

    3) Product Images from "Methotrexate sensitizes drug-resistant metastatic melanoma cells to BRAF V600E inhibitors dabrafenib and encorafenib"

    Article Title: Methotrexate sensitizes drug-resistant metastatic melanoma cells to BRAF V600E inhibitors dabrafenib and encorafenib

    Journal: Oncotarget

    doi: 10.18632/oncotarget.24341

    MAPK activation and DNA damage checkpoint induction following MTX + dabrafenib combination treatments ( A ) Western blot of p-ERK1/2 (Thr202/Tyr204) expression in differentially treated SK-MEL-28 and SK-MEL-28VR1 cells. β-actin used as loading control. 30 µg of protein loaded in each lane. ( B ) Western blot of RPA70 and p-CDK1 (Tyr15) expression in differentially treated SK-MEL-28 and SK-MEL28VR1 cells. β-actin used as loading control. 30 µg of protein loaded in each lane. ( C ) Western of cleaved PARP1 and cleaved caspase 3 expression in differentially treated SK-MEL-28 and SK-MEL-28VR1 cells. β-actin used as loading control. 30 µg of protein loaded in each lane.
    Figure Legend Snippet: MAPK activation and DNA damage checkpoint induction following MTX + dabrafenib combination treatments ( A ) Western blot of p-ERK1/2 (Thr202/Tyr204) expression in differentially treated SK-MEL-28 and SK-MEL-28VR1 cells. β-actin used as loading control. 30 µg of protein loaded in each lane. ( B ) Western blot of RPA70 and p-CDK1 (Tyr15) expression in differentially treated SK-MEL-28 and SK-MEL28VR1 cells. β-actin used as loading control. 30 µg of protein loaded in each lane. ( C ) Western of cleaved PARP1 and cleaved caspase 3 expression in differentially treated SK-MEL-28 and SK-MEL-28VR1 cells. β-actin used as loading control. 30 µg of protein loaded in each lane.

    Techniques Used: Activation Assay, Western Blot, Expressing

    Related Articles

    Western Blot:

    Article Title: The ubiquitin ligase Cullin-1 associates with chromatin and regulates transcription of specific c-MYC target genes
    Article Snippet: Proteins were separated using the Bio-Rad PowerPac and 1X Tris/glycine/SDS buffer (Bio-Rad, #1610732) and subsequently transferred to Trans-Blot Turbo transfer pack membranes (Bio-Rad, #1704156) using Trans-Blot Turbo transfer system (Bio-Rad). .. Western blot analysis against 3xFLAG was carried out using monoclonal anti-FLAG M2 antibody produced in mouse (Sigma-Aldrich, #F1804-1MG) with β-actin rabbit monoclonal antibody (Cell Signaling Technology, clone D6A8) as a loading control. .. Western blot against CUL1 was performed with recombinant anti-Cullin1/CUL-1 antibody (Abcam, ab75817).

    Produced:

    Article Title: The ubiquitin ligase Cullin-1 associates with chromatin and regulates transcription of specific c-MYC target genes
    Article Snippet: Proteins were separated using the Bio-Rad PowerPac and 1X Tris/glycine/SDS buffer (Bio-Rad, #1610732) and subsequently transferred to Trans-Blot Turbo transfer pack membranes (Bio-Rad, #1704156) using Trans-Blot Turbo transfer system (Bio-Rad). .. Western blot analysis against 3xFLAG was carried out using monoclonal anti-FLAG M2 antibody produced in mouse (Sigma-Aldrich, #F1804-1MG) with β-actin rabbit monoclonal antibody (Cell Signaling Technology, clone D6A8) as a loading control. .. Western blot against CUL1 was performed with recombinant anti-Cullin1/CUL-1 antibody (Abcam, ab75817).

    Incubation:

    Article Title: Endocrine sensitivity of estrogen receptor‐positive breast cancer is negatively correlated with aspartate‐β‐hydroxylase expression
    Article Snippet: .. Membranes were incubated at 4°C overnight with primary monoclonal antibodies including mouse anti‐ASPH (FB‐50), rabbit anti‐β actin (D6A8; Cell Signaling Technology), rabbit anti‐ERK1/2 (137F5; Cell Signaling Technology), rabbit anti‐P‐ERK1/2 (Thr202/Tyr204; D13.14.4E; Cell Signaling Technology), rabbit anti‐Akt (C67E7; Cell Signaling Technology), and rabbit anti‐P‐Akt (Ser473; D9E; Cell Signaling Technology). .. Membranes were incubated with HRP‐conjugated anti‐mouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) or anti‐rabbit IgG antibody (Cell Signaling Technology), followed by chemiluminescence detection.

    Article Title: Partial Depletion of Peripheral M1 Macrophages Reverses Motor Deficits in MPTP-Treated Mouse by Suppressing Neuroinflammation and Dopaminergic Neurodegeneration
    Article Snippet: Proteins were separated by SDS-PAGE and transferred onto a nitrocellulose membrane. .. The membranes were blocked with 5% non-fat dried milk for 1 h and incubated with rabbit monoclonal anti-iNOS (cat.no.136918, 1:1,000, Abcam; Lizano et al., ), rabbit monoclonal anti-Arg-1 (cat.no.93668, 1:1,000, Cell Signaling Technology; Mogami et al., ), rabbit monoclonal anti-p-NF-κB (cat.no.3033, 1:1,000, Cell Signaling Technology; Yan et al., ), rat monoclonal anti-MHC II (cat.no.139365, 1:1,000, Abcam; Mori et al., ) and rabbit monoclonal anti-β-actin (cat.no.4970, 1:1,000, Cell Signaling Technology; Liu et al., ) overnight at 4°C. .. After being washed with Tris-buffered saline with TWEEN 20 (TBST) buffer, the membranes were incubated with anti-rabbit (cat. no. 7074, 1:2,000, Cell Signaling Technology) or anti-rat (cat. no. 7077, 1:2,000, Cell Signaling Technology) horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature and then subjected to chemiluminescent detection according to the manufacturer's instructions (Millipore).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Partial Depletion of Peripheral M1 Macrophages Reverses Motor Deficits in MPTP-Treated Mouse by Suppressing Neuroinflammation and Dopaminergic Neurodegeneration
    Article Snippet: Proteins were separated by SDS-PAGE and transferred onto a nitrocellulose membrane. .. The membranes were blocked with 5% non-fat dried milk for 1 h and incubated with rabbit monoclonal anti-iNOS (cat.no.136918, 1:1,000, Abcam; Lizano et al., ), rabbit monoclonal anti-Arg-1 (cat.no.93668, 1:1,000, Cell Signaling Technology; Mogami et al., ), rabbit monoclonal anti-p-NF-κB (cat.no.3033, 1:1,000, Cell Signaling Technology; Yan et al., ), rat monoclonal anti-MHC II (cat.no.139365, 1:1,000, Abcam; Mori et al., ) and rabbit monoclonal anti-β-actin (cat.no.4970, 1:1,000, Cell Signaling Technology; Liu et al., ) overnight at 4°C. .. After being washed with Tris-buffered saline with TWEEN 20 (TBST) buffer, the membranes were incubated with anti-rabbit (cat. no. 7074, 1:2,000, Cell Signaling Technology) or anti-rat (cat. no. 7077, 1:2,000, Cell Signaling Technology) horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature and then subjected to chemiluminescent detection according to the manufacturer's instructions (Millipore).

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  • 86
    Cell Signaling Technology Inc rabbit anti β actin
    Genetic ablation of Nrf2 reduces angiogenic sprouting and vascular density. ( A ) Blood vessels were visualized by PECAM-1 staining of retinas at P5. Decreased vascular density ( B ), branch points ( C ), sprouts ( D ), and filopodia ( E ) were observed in Nrf2 − / − retina ( n = 6). ( F and G ) Isolectin B4 (green) and BrdU labeling (red) of retinas at P5 demonstrate reduced EC proliferation in Nrf2 − / − ( n = 4). (Scale bar, 50 µm.) ( H ) NG2 + pericytes in capillaries or SMA + vascular smooth muscle cells in arterioles were indistinguishable between WT ( Nrf2 +/+ ) and Nrf2 − / − retinas at P5. (Scale bar, 100 µm.) ( I ) Nrf2 is expressed in developing blood vessels, and strong expression was observed in the sprouting vascular front. Arrowheads indicate Nrf2 expression in tip cells and stalk cells (A, artery; V, vein). ( J ) Nrf2 expression was increased in the ganglion cell layer (GCL) of retina at P5 compared with P0. Nrf2 was highly expressed in the vessels and surrounding area (arrowheads). Nrf2 localization in the nucleus was apparent (arrows). HV, hyaloid vessels; INL, inner nuclear layer. ( K ) Immunoblot analysis of Nrf2 in the retinas of WT mice at P0 and P5. <t>β-Actin</t> was used as a loading control ( n = 3). ( L ) Quantitative RT-PCR analysis of Nrf2 and Nrf2 target genes in the retinas of WT mice at P0 and P5 ( n = 5). Data are presented as mean ± SEM (** P
    Rabbit Anti β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti β actin/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti β actin - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc ãŽâ² actin
    CD1d deficiency protects mice from challenge with TLR ligands and pathogen infection. a ELISA of TNF, IL-6 and <t>IFN-β</t> in sera of Cd1d +/+ and Cd1d –/– mice ( n  = 5 per group) 2 h after intraperitoneal injection
    ãŽâ² Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ãŽâ² actin/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ãŽâ² actin - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc filamin a
    <t>Filamin</t> A and NEDD4L protein expression levels are altered in cystitis. ( a , c ) Upon CYP treatment, Filamin A protein expression level in the bladder was significantly elevated, while NEDD4L protein expression level was significantly downregulated (data represent the mean±s.d., N =6, ** P
    Filamin A, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filamin a/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    filamin a - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Genetic ablation of Nrf2 reduces angiogenic sprouting and vascular density. ( A ) Blood vessels were visualized by PECAM-1 staining of retinas at P5. Decreased vascular density ( B ), branch points ( C ), sprouts ( D ), and filopodia ( E ) were observed in Nrf2 − / − retina ( n = 6). ( F and G ) Isolectin B4 (green) and BrdU labeling (red) of retinas at P5 demonstrate reduced EC proliferation in Nrf2 − / − ( n = 4). (Scale bar, 50 µm.) ( H ) NG2 + pericytes in capillaries or SMA + vascular smooth muscle cells in arterioles were indistinguishable between WT ( Nrf2 +/+ ) and Nrf2 − / − retinas at P5. (Scale bar, 100 µm.) ( I ) Nrf2 is expressed in developing blood vessels, and strong expression was observed in the sprouting vascular front. Arrowheads indicate Nrf2 expression in tip cells and stalk cells (A, artery; V, vein). ( J ) Nrf2 expression was increased in the ganglion cell layer (GCL) of retina at P5 compared with P0. Nrf2 was highly expressed in the vessels and surrounding area (arrowheads). Nrf2 localization in the nucleus was apparent (arrows). HV, hyaloid vessels; INL, inner nuclear layer. ( K ) Immunoblot analysis of Nrf2 in the retinas of WT mice at P0 and P5. β-Actin was used as a loading control ( n = 3). ( L ) Quantitative RT-PCR analysis of Nrf2 and Nrf2 target genes in the retinas of WT mice at P0 and P5 ( n = 5). Data are presented as mean ± SEM (** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Nrf2 acts cell-autonomously in endothelium to regulate tip cell formation and vascular branching

    doi: 10.1073/pnas.1309276110

    Figure Lengend Snippet: Genetic ablation of Nrf2 reduces angiogenic sprouting and vascular density. ( A ) Blood vessels were visualized by PECAM-1 staining of retinas at P5. Decreased vascular density ( B ), branch points ( C ), sprouts ( D ), and filopodia ( E ) were observed in Nrf2 − / − retina ( n = 6). ( F and G ) Isolectin B4 (green) and BrdU labeling (red) of retinas at P5 demonstrate reduced EC proliferation in Nrf2 − / − ( n = 4). (Scale bar, 50 µm.) ( H ) NG2 + pericytes in capillaries or SMA + vascular smooth muscle cells in arterioles were indistinguishable between WT ( Nrf2 +/+ ) and Nrf2 − / − retinas at P5. (Scale bar, 100 µm.) ( I ) Nrf2 is expressed in developing blood vessels, and strong expression was observed in the sprouting vascular front. Arrowheads indicate Nrf2 expression in tip cells and stalk cells (A, artery; V, vein). ( J ) Nrf2 expression was increased in the ganglion cell layer (GCL) of retina at P5 compared with P0. Nrf2 was highly expressed in the vessels and surrounding area (arrowheads). Nrf2 localization in the nucleus was apparent (arrows). HV, hyaloid vessels; INL, inner nuclear layer. ( K ) Immunoblot analysis of Nrf2 in the retinas of WT mice at P0 and P5. β-Actin was used as a loading control ( n = 3). ( L ) Quantitative RT-PCR analysis of Nrf2 and Nrf2 target genes in the retinas of WT mice at P0 and P5 ( n = 5). Data are presented as mean ± SEM (** P

    Article Snippet: The following antibodies were used: rabbit anti-Nrf2, mouse anti-GAPDH (Abcam), rabbit anti–β-actin, rabbit anti-NICD, rabbit anti–phospho-Akt, mouse anti-Akt1, mouse anti–phospho-p44/42 MAPK (Erk1/2), rabbit anti-p44/42 MAPK (Erk1/2), and rabbit anti-Dll4 (Cell Signaling Technology).

    Techniques: Staining, Labeling, Expressing, Mouse Assay, Quantitative RT-PCR

    Western blot analysis of poly (adenosine diphosphate-ribose) polymerase in A549, PC3 and H1299 cell lines. β-actin was used as loading control. The treatment was conducted for 24 h. Vertical and horizontal lanes indicate separate blots. PQE, Pelargonium quercetorum extract; PARP, poly (adenosine diphosphate-ribose) polymerase.

    Journal: Oncology Letters

    Article Title: Pelargonium quercetorum Agnew induces apoptosis without PARP or cytokeratin 18 cleavage in non-small cell lung cancer cell lines

    doi: 10.3892/ol.2016.4779

    Figure Lengend Snippet: Western blot analysis of poly (adenosine diphosphate-ribose) polymerase in A549, PC3 and H1299 cell lines. β-actin was used as loading control. The treatment was conducted for 24 h. Vertical and horizontal lanes indicate separate blots. PQE, Pelargonium quercetorum extract; PARP, poly (adenosine diphosphate-ribose) polymerase.

    Article Snippet: Western blotting was performed using rabbit anti-β-actin and anti-PARP monoclonal antibodies at 1:1,000 dilution (catalog nos., 4970 and 9532, respectively; Cell Signaling Technology, Inc., Danvers, MA, USA) in 5% (w/v) bovine serum albumin (Amresco, LLC, Solon, OH, USA).

    Techniques: Western Blot

    CD1d deficiency protects mice from challenge with TLR ligands and pathogen infection. a ELISA of TNF, IL-6 and IFN-β in sera of Cd1d +/+ and Cd1d –/– mice ( n  = 5 per group) 2 h after intraperitoneal injection

    Journal: Cell Research

    Article Title: Glycolipid iGb3 feedback amplifies innate immune responses via CD1d reverse signaling

    doi: 10.1038/s41422-018-0122-7

    Figure Lengend Snippet: CD1d deficiency protects mice from challenge with TLR ligands and pathogen infection. a ELISA of TNF, IL-6 and IFN-β in sera of Cd1d +/+ and Cd1d –/– mice ( n  = 5 per group) 2 h after intraperitoneal injection

    Article Snippet: Antibodies to ERK phosphorylated at Thr202/Tyr204 (9106); JNK phosphorylated at Thr183/Tyr185 (9255); p38 phosphorylated at Thr180/Tyr182 (9211); NF-κB p65 phosphorylated at Ser536 (3033); IκBα phosphorylated at Ser32/36 (9246); IRF3 phosphorylated at Ser396 (4947); IKKα/β phosphorylated at Ser177/Ser181 (2697); TBK1 phosphorylated at Ser172 (5483); Pyk2 phosphorylated at Tyr402 (3291); Pyk2 (3292), IKKβ (8943), EEA1 (2411); Myc-tag (2272), hemagglutinin-tag (2367); and β-actin (3700) were obtained from Cell Signaling Technology.

    Techniques: Mouse Assay, Infection, Enzyme-linked Immunosorbent Assay, Injection

    Filamin A and NEDD4L protein expression levels are altered in cystitis. ( a , c ) Upon CYP treatment, Filamin A protein expression level in the bladder was significantly elevated, while NEDD4L protein expression level was significantly downregulated (data represent the mean±s.d., N =6, ** P

    Journal: Experimental & Molecular Medicine

    Article Title: Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice

    doi: 10.1038/emm.2017.31

    Figure Lengend Snippet: Filamin A and NEDD4L protein expression levels are altered in cystitis. ( a , c ) Upon CYP treatment, Filamin A protein expression level in the bladder was significantly elevated, while NEDD4L protein expression level was significantly downregulated (data represent the mean±s.d., N =6, ** P

    Article Snippet: Filamin A is known to interact with the C terminus of the HCN1 channel via a 22-amino-acid region and to facilitate HCN1 channel membrane trafficking.

    Techniques: Expressing