β actin c4  (Millipore)

Journal: Oncogene

Article Title: Integrin α1β1 expression is controlled by c-MYC in colorectal cancer cells

doi: 10.1038/onc.2015.231

Figure Lengend Snippet: MYC inhibition downregulates ITGA1 expression at the mRNA and protein levels in colorectal cancer cells. ( a ) T84, HT29 and SW480 cells were treated with the MYC inhibitor 10058-F4 used at 50 μ M in dimethyl sulfoxide (DMSO; MYCi) or with DMSO alone for the indicated times. Real-time quantitative PC R (qPCR) levels are indicated as fold changes of ITGA1 relative to the control and normalized to RPLP0 used as housekeeping gene. ( b ) Representative western blot and densitometric analyses of MYC and ITGA1 expression after treatment of T84, HT29 and SW480 cells with MYCi as in ( a ). β-Actin (ACTB) was used as loading control. All experiments were performed in triplicate and repeated three times. * P

Article Snippet: Membranes were then incubated overnight at 4 °C with the following primary antibodies: anti-human integrin α1/CD49a (1:2000, AF5676, R & D Systems, Minneapolis, MN, USA), anti-c-Myc (1:5000, Y69, ab32072, Abcam, Toronto, ON, Canada), anti-β1 (1:1000, BD Biosciences, Mississauga, ON, Canada) and anti-β-actin (1:80 000, C4, Millipore, Etobicoke, ON, Canada) as endogenous loading control.

Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal:

Article Title: Androgens act synergistically to enhance estrogen‐induced upregulation of human tissue kallikreins 10, 11, and 14 in breast cancer cells via a membrane bound androgen receptor), Androgens act synergistically to enhance estrogen‐induced upregulation of human tissue kallikreins 10, 11, and 14 in breast cancer cells via a membrane bound androgen receptor

doi: 10.1016/j.molonc.2008.01.001

Figure Lengend Snippet: Western blot analysis of hormone receptors. Cell lysates from BT474 were analyzed for changes in androgen (AR), estrogen (ER) and glucocorticoid (GR) and progesterone (PGR) receptor levels. β‐ACTIN is used as the loading control for this

Article Snippet: The antibodies used for Western blot analysis included, AR (N‐20), ERα (D‐12), and PGR (AB‐52), and β‐ACTIN (C4) from EMD Biosciences Inc., San Diego, CA and GR (Clone 41) from BD Biosciences, San Jose, CA.

Techniques: Western Blot

Journal: Journal of Virology

Article Title: Suppression of Transcription Factor Early Growth Response 1 Reduces Herpes Simplex Virus 1-Induced Corneal Disease in Mice

doi: 10.1128/JVI.00505-12

Figure Lengend Snippet: HSV-1 infection enhances Egr-1 expression in the cornea. Corneas (A) or eyes (B) of C57BL/6 mice mock infected or infected with HSV-1 were harvested at the indicated days p.i. (A) Mouse corneas were assayed by Western blotting to detect Egr-1 and β-actin

Article Snippet: Membranes were probed with antibodies against mouse Egr-1 (Santa Cruz Biotechnology, Inc.) or mouse β-actin (clone C4; Millipore), followed by appropriate horseradish peroxidase-conjugated secondary antibodies.

Techniques: Infection, Expressing, Mouse Assay, Western Blot