Article Title: Integrin α1β1 expression is controlled by c-MYC in colorectal cancer cells
Figure Lengend Snippet: MYC inhibition downregulates ITGA1 expression at the mRNA and protein levels in colorectal cancer cells. ( a ) T84, HT29 and SW480 cells were treated with the MYC inhibitor 10058-F4 used at 50 μ M in dimethyl sulfoxide (DMSO; MYCi) or with DMSO alone for the indicated times. Real-time quantitative PC R (qPCR) levels are indicated as fold changes of ITGA1 relative to the control and normalized to RPLP0 used as housekeeping gene. ( b ) Representative western blot and densitometric analyses of MYC and ITGA1 expression after treatment of T84, HT29 and SW480 cells with MYCi as in ( a ). β-Actin (ACTB) was used as loading control. All experiments were performed in triplicate and repeated three times. * P
Article Snippet: Membranes were then incubated overnight at 4 °C with the following primary antibodies: anti-human integrin α1/CD49a (1:2000, AF5676, R & D Systems, Minneapolis, MN, USA), anti-c-Myc (1:5000, Y69, ab32072, Abcam, Toronto, ON, Canada), anti-β1 (1:1000, BD Biosciences, Mississauga, ON, Canada) and anti-β-actin (1:80 000, C4, Millipore, Etobicoke, ON, Canada) as endogenous loading control.
Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Western Blot