α n acetylgalactosaminidase  (New England Biolabs)


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    Name:
    alpha N Acetyl galactosaminidase
    Description:
    alpha N Acetyl galactosaminidase 15 000 units
    Catalog Number:
    p0734l
    Price:
    539
    Size:
    15 000 units
    Category:
    Glycosidases
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    Structured Review

    New England Biolabs α n acetylgalactosaminidase
    alpha N Acetyl galactosaminidase
    alpha N Acetyl galactosaminidase 15 000 units
    https://www.bioz.com/result/α n acetylgalactosaminidase/product/New England Biolabs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    α n acetylgalactosaminidase - by Bioz Stars, 2020-08
    93/100 stars

    Images

    1) Product Images from "The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface *"

    Article Title: The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M113.476531

    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red
    Figure Legend Snippet: Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Techniques Used: Binding Assay

    2) Product Images from "Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures"

    Article Title: Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures

    Journal: Glycobiology

    doi: 10.1093/glycob/cwt007

    ( A ) EIC of m/z 548.2 from the BMO pool showing two main isomers. ( B ) EIC of m/z 548.2 after digestion with α N -acetylgalactosaminidase. ( C ) EIC of m/z 548.2 after digestion with β N -acetylhexosaminidase. ( D ) Magnification on base line
    Figure Legend Snippet: ( A ) EIC of m/z 548.2 from the BMO pool showing two main isomers. ( B ) EIC of m/z 548.2 after digestion with α N -acetylgalactosaminidase. ( C ) EIC of m/z 548.2 after digestion with β N -acetylhexosaminidase. ( D ) Magnification on base line

    Techniques Used:

    Related Articles

    Transfection:

    Article Title: Binding to histo-blood group antigen-expressing bacteria protects human norovirus from acute heat stress
    Article Snippet: .. In order to control for the specificity of the anti-HBGA antibodies and of VLPs binding to bacteria, E. coli strains LMG8223 was treated by α-N -acetylgalactosaminidase (New England Biolabs) at 37°C for 1 h. Chinese hamster ovary (CHO) cells, transfected with rat α1,2- fucosyltransferase B cDNA and the rat A enzyme cDNA as described previously , were utilized as a positive control for this enzymatic treatment. .. E. coli LFMFP861 was treated by the α-galactosidase from coffee beans (Sigma) at 37°C for 1 h. The A and B antigens expression on treated and untreated cells were detected by the use of antibody #21 and #49, respectively.

    Binding Assay:

    Article Title: Binding to histo-blood group antigen-expressing bacteria protects human norovirus from acute heat stress
    Article Snippet: .. In order to control for the specificity of the anti-HBGA antibodies and of VLPs binding to bacteria, E. coli strains LMG8223 was treated by α-N -acetylgalactosaminidase (New England Biolabs) at 37°C for 1 h. Chinese hamster ovary (CHO) cells, transfected with rat α1,2- fucosyltransferase B cDNA and the rat A enzyme cDNA as described previously , were utilized as a positive control for this enzymatic treatment. .. E. coli LFMFP861 was treated by the α-galactosidase from coffee beans (Sigma) at 37°C for 1 h. The A and B antigens expression on treated and untreated cells were detected by the use of antibody #21 and #49, respectively.

    Positive Control:

    Article Title: Binding to histo-blood group antigen-expressing bacteria protects human norovirus from acute heat stress
    Article Snippet: .. In order to control for the specificity of the anti-HBGA antibodies and of VLPs binding to bacteria, E. coli strains LMG8223 was treated by α-N -acetylgalactosaminidase (New England Biolabs) at 37°C for 1 h. Chinese hamster ovary (CHO) cells, transfected with rat α1,2- fucosyltransferase B cDNA and the rat A enzyme cDNA as described previously , were utilized as a positive control for this enzymatic treatment. .. E. coli LFMFP861 was treated by the α-galactosidase from coffee beans (Sigma) at 37°C for 1 h. The A and B antigens expression on treated and untreated cells were detected by the use of antibody #21 and #49, respectively.

    Concentration Assay:

    Article Title: Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity
    Article Snippet: .. To examine the specificity of EtpA-induced agglutination of RBCs expressing the A antigen, agglutination assays were performed in the presence of excess terminal sugars specific to individual blood types: GalNAc (blood group A), Gal (blood group B), or control GlcNAc at 50 mM final concentration. α- N -acetylgalactosaminidase (P0734S; New England Biolabs) was used to remove terminal GalNAc from the A blood group antigen by incubating 50 μl of a 1% RBC suspension with 20 U α- N -acetylgalactosaminidase for 4 hours at 37°C. ..

    Article Title: Histo-Blood Group Antigen-Like Substances of Human Enteric Bacteria as Specific Adsorbents for Human Noroviruses
    Article Snippet: .. One hundred microliters of reaction mixture contained 88 μl of extracted EPS, 10 μl of 10× G7 reaction buffer, and 1 μl of α- N -acetyl-galactosaminidase to yield a final concentration of 0.2 U/ml (New England BioLabs, Inc., Japan), and 1 μl of 10 mg/ml BSA. ..

    other:

    Article Title: Bovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens
    Article Snippet: NaIO4 and NA, from Sigma-Aldrich, were dissolved in phosphate-buffered saline (PBS; pH 7.2). α1,2-l -Fucosidase from Corynebacterium (TaKaRa Bio Inc., Kyoto, Japan), α1,3/4-l -fucosidase from Streptomyces (TaKaRa Bio Inc.), α-galactosidase from Coffea arabica (Sigma-Aldrich), α-N -acetylgalactosaminidase from Chryseobacterium meningosepticum (New England BioLabs, Inc., MA, USA), and UEA-1 (Vector Laboratories, Burlingame, CA, USA) were diluted in PBS.

    Expressing:

    Article Title: Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity
    Article Snippet: .. To examine the specificity of EtpA-induced agglutination of RBCs expressing the A antigen, agglutination assays were performed in the presence of excess terminal sugars specific to individual blood types: GalNAc (blood group A), Gal (blood group B), or control GlcNAc at 50 mM final concentration. α- N -acetylgalactosaminidase (P0734S; New England Biolabs) was used to remove terminal GalNAc from the A blood group antigen by incubating 50 μl of a 1% RBC suspension with 20 U α- N -acetylgalactosaminidase for 4 hours at 37°C. ..

    Recombinant:

    Article Title: Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures
    Article Snippet: .. Recombinant β1-3,6 galactosidase, α1-3,6 galactosidase, β N -acetylhexosaminidase, α N -acetylgalactosaminidase, β N -acetylhexosaminidase, β N -acetylglucosaminidase and α2-3 neuraminidase were purchased from New England Biolabs (Ispwich, MA). β1-4 galactosidase was purchased from Prozyme (Hayward, CA). .. All other reagents were of the analytical or HPLC grade.

    Article Title: AGO61-dependent GlcNAc modification primes the formation of functional glycans on ?-dystroglycan
    Article Snippet: .. Glycosidase treatments Recombinant α-DG was treated with β-N -acetylhexosaminidase (New England Biolabs), β-N -acetylglucosaminidase (New England Biolabs), or α-N -acetylgalactosaminidase (New England Biolabs) according to the manufacturer's instructions. .. These specimens were subjected to Western blot analysis with an anti-O -GlcNAc antibody, CTD110.6.

    Agglutination:

    Article Title: Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity
    Article Snippet: .. To examine the specificity of EtpA-induced agglutination of RBCs expressing the A antigen, agglutination assays were performed in the presence of excess terminal sugars specific to individual blood types: GalNAc (blood group A), Gal (blood group B), or control GlcNAc at 50 mM final concentration. α- N -acetylgalactosaminidase (P0734S; New England Biolabs) was used to remove terminal GalNAc from the A blood group antigen by incubating 50 μl of a 1% RBC suspension with 20 U α- N -acetylgalactosaminidase for 4 hours at 37°C. ..

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    New England Biolabs α n acetylgalactosaminidase
    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N <t>-acetylgalactosaminidase</t> treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red
    α N Acetylgalactosaminidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α n acetylgalactosaminidase/product/New England Biolabs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    α n acetylgalactosaminidase - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    99
    New England Biolabs endo α n acetylgalactosaminidase
    SDS-PAGE of Ni-purified recombinant glucanases. (A) Pa Cel6A and Tr Cel6A. Lanes 1 and 2, recombinant proteins; lanes 3, treatment with <t>endo-α-</t> N <t>-acetylgalactosaminidase</t> ( O -glycosidase); lanes 4, treatment with Endo H. (B) Pa Cel6B and Pa Cel6C. Lanes
    Endo α N Acetylgalactosaminidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endo α n acetylgalactosaminidase/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    endo α n acetylgalactosaminidase - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Journal: The Journal of Biological Chemistry

    Article Title: The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface *

    doi: 10.1074/jbc.M113.476531

    Figure Lengend Snippet: Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Article Snippet: PNGase F (from Flavobacterium meningosepticum ), O -glycosidase (cloned from Enterococcus faecalis and expressed in Escherichia coli ), α- N -acetylgalactosaminidase (cloned from Chryseobacterium meningosepticum and expressed in E. coli ), and β1,3-galactosidase (cloned from Xanthomonas manihotis and expressed in E. coli ) were obtained from New England Biolabs (Ipswich, MA).

    Techniques: Binding Assay

    SDS-PAGE of Ni-purified recombinant glucanases. (A) Pa Cel6A and Tr Cel6A. Lanes 1 and 2, recombinant proteins; lanes 3, treatment with endo-α- N -acetylgalactosaminidase ( O -glycosidase); lanes 4, treatment with Endo H. (B) Pa Cel6B and Pa Cel6C. Lanes

    Journal: Applied and Environmental Microbiology

    Article Title: Insights into Exo- and Endoglucanase Activities of Family 6 Glycoside Hydrolases from Podospora anserina

    doi: 10.1128/AEM.00327-13

    Figure Lengend Snippet: SDS-PAGE of Ni-purified recombinant glucanases. (A) Pa Cel6A and Tr Cel6A. Lanes 1 and 2, recombinant proteins; lanes 3, treatment with endo-α- N -acetylgalactosaminidase ( O -glycosidase); lanes 4, treatment with Endo H. (B) Pa Cel6B and Pa Cel6C. Lanes

    Article Snippet: Deglycosylation of 2 μg recombinant protein was performed with 2 μl of either endo-α- N -acetylgalactosaminidase and 2 μl neuraminidase or 2 μl endoglycosidase H (Endo H) (all from New England BioLabs) for 2 h at 37°C, according to the manufacturer's instructions.

    Techniques: SDS Page, Purification, Recombinant

    pUL11 is a surface expressed glycoprotein. (A) Predicted structure of the UL11 protein. (B) Flow cytometry with a pUL11-specific rabbit antiserum (black lines) or pre-immune serum (grey lines) of HFF or A549 cells transduced with recombinant adenoviruses expressing UL11 and GFP (rAdV UL11) or GFP alone (rAdV GFP). Gating was on GFP positive cells. (C) Confocal microscopy of HFF and A549 cells transduced as in B) and labeled at the cell surface with the anti-pUL11 serum and an Alexa-568 conjugated anti-rabbit antibody (shown in red). Size bar, 20 µm. (D) Immunoblot with a mouse anti-V5 antibody of lysates of rAdV UL11, rAdV GFP or mock transduced A549 cells. (E) Lysates of rAdV UL11 transduced A549 cells were treated with peptide N-glycosidase F (PNGase F), endo-α-N-acetylgalactosaminidase (O-glycosidase) or untreated as indicated. Immunoblotting was performed as in (D). (D, E) Left margin, molecular mass (in kDa); right margin, the arrowhead and the bracket indicate low and high molecular weight forms of pUL11.

    Journal: PLoS Pathogens

    Article Title: The Human Cytomegalovirus UL11 Protein Interacts with the Receptor Tyrosine Phosphatase CD45, Resulting in Functional Paralysis of T Cells

    doi: 10.1371/journal.ppat.1002432

    Figure Lengend Snippet: pUL11 is a surface expressed glycoprotein. (A) Predicted structure of the UL11 protein. (B) Flow cytometry with a pUL11-specific rabbit antiserum (black lines) or pre-immune serum (grey lines) of HFF or A549 cells transduced with recombinant adenoviruses expressing UL11 and GFP (rAdV UL11) or GFP alone (rAdV GFP). Gating was on GFP positive cells. (C) Confocal microscopy of HFF and A549 cells transduced as in B) and labeled at the cell surface with the anti-pUL11 serum and an Alexa-568 conjugated anti-rabbit antibody (shown in red). Size bar, 20 µm. (D) Immunoblot with a mouse anti-V5 antibody of lysates of rAdV UL11, rAdV GFP or mock transduced A549 cells. (E) Lysates of rAdV UL11 transduced A549 cells were treated with peptide N-glycosidase F (PNGase F), endo-α-N-acetylgalactosaminidase (O-glycosidase) or untreated as indicated. Immunoblotting was performed as in (D). (D, E) Left margin, molecular mass (in kDa); right margin, the arrowhead and the bracket indicate low and high molecular weight forms of pUL11.

    Article Snippet: Cell lysates or purified proteins were boiled for 5 min in denaturing buffer (0.5% SDS, 0.5% 2-mercaptoethanol) before being treated with N-glycosidase F (4 U) (Roche) or Endo-α-N-acetylgalactosaminidase (2,000 U) and neuraminidase (100 U) (New England Biolabs) in 500 mM sodium phosphate buffer pH 7.6 containing 1% NP40 for 2 h or overnight at 37°C.

    Techniques: Flow Cytometry, Cytometry, Transduction, Recombinant, Expressing, Confocal Microscopy, Labeling, Molecular Weight

    Immunoblot analysis of endogenous bovine IgD. (a) Western blot of splenic membrane and serum proteins. (b) Western blot analysis of the IgD glycosylation in bovine serum. 1, Untreated serum proteins; 2, endo-α-N-acetylgalactosaminidase-treated serum proteins; 3, PNGase-treated serum proteins. The primary antibody 13C2 was diluted at 1∶200 or 1∶400. (c) Serum immunoblot analysis with mAb 13C2 and anti-bovine IgM polyclonal antibody under nonreducing conditions. The arrow indicates the monomer of IgD. (d) Immunoblot detection of serum IgD and IgM in differently aged cows. 1, 60 days old; 2, 180 days; 3, 1 year; 4, 2 years; 5, 3 years; 6, 4 years; 7, 5 years; 8, 6 years. The arrows indicate the IgD heavy chain.

    Journal: PLoS ONE

    Article Title: Expressional Analysis of Immunoglobulin D in Cattle (Bos taurus), a Large Domesticated Ungulate

    doi: 10.1371/journal.pone.0044719

    Figure Lengend Snippet: Immunoblot analysis of endogenous bovine IgD. (a) Western blot of splenic membrane and serum proteins. (b) Western blot analysis of the IgD glycosylation in bovine serum. 1, Untreated serum proteins; 2, endo-α-N-acetylgalactosaminidase-treated serum proteins; 3, PNGase-treated serum proteins. The primary antibody 13C2 was diluted at 1∶200 or 1∶400. (c) Serum immunoblot analysis with mAb 13C2 and anti-bovine IgM polyclonal antibody under nonreducing conditions. The arrow indicates the monomer of IgD. (d) Immunoblot detection of serum IgD and IgM in differently aged cows. 1, 60 days old; 2, 180 days; 3, 1 year; 4, 2 years; 5, 3 years; 6, 4 years; 7, 5 years; 8, 6 years. The arrows indicate the IgD heavy chain.

    Article Snippet: To examine the glycosylation of IgM and IgD, the serum was treated with the peptides N-glycosidase F (PNGase F, NEB) and endo-α-N-acetylgalactosaminidase (NEB) following the manufacturer's instructions.

    Techniques: Western Blot