o glycosidase  (New England Biolabs)


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  • 99
    Name:
    O Glycosidase
    Description:
    O Glycosidase 10 000 000 units
    Catalog Number:
    P0733L
    Price:
    550
    Category:
    Glycosidases
    Size:
    10 000 000 units
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    Structured Review

    New England Biolabs o glycosidase
    O Glycosidase
    O Glycosidase 10 000 000 units
    https://www.bioz.com/result/o glycosidase/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    o glycosidase - by Bioz Stars, 2021-05
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Versatile Fungal Polyphenol Oxidase with Chlorophenol Bioremediation Potential: Characterization and Protein Engineering
    Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed to examine the homogeneity and molecular weight of the purified enzyme. .. In order to investigate potential N -glycosylation on the recombinant protein, endoglycosidase treatment was performed by Endo H enzyme (NEB, USA) under native conditions, according to the manufacturer's manual. ..

    Incubation:

    Article Title: Single Mutations in the VP2 300 Loop Region of the Three-Fold Spike of the Carnivore Parvovirus Capsid Can Determine Host Range
    Article Snippet: The relative quantitation of the sugar glycan isoforms of N-linked peptide ions was carried out based on precursor ion peak areas under the assumption that all sugar glycan isoforms linked to the same core peptide have identical or similar ionization efficiencies, as reported previously ( ). .. To test if endoglycosidase H (endo H) treatment would specifically remove glycans from the dog and cat TfRs, we incubated 13.5 μg of FPLC-purified, denatured TfR with 4,000 U of endo Hf (New England BioLabs, Ipswich, MA) overnight according to the manufacturer's protocols and then analyzed the electrophoretic mobility by SDS-PAGE. ..

    Article Title: Human cytomegalovirus gH stability and trafficking are regulated by ER-associated degradation and transmembrane architecture
    Article Snippet: For proteasome inhibition experiments, cells were treated for 12 hours with the proteasome inhibitor ZL3 VS (2.5µM). .. For experiments involving Endoglycosidase H treatment, total cell lysates were incubated with 500 units of Endoglycosidase Hf (New England Biolabs) for 2 hours. ..

    Fast Protein Liquid Chromatography:

    Article Title: Single Mutations in the VP2 300 Loop Region of the Three-Fold Spike of the Carnivore Parvovirus Capsid Can Determine Host Range
    Article Snippet: The relative quantitation of the sugar glycan isoforms of N-linked peptide ions was carried out based on precursor ion peak areas under the assumption that all sugar glycan isoforms linked to the same core peptide have identical or similar ionization efficiencies, as reported previously ( ). .. To test if endoglycosidase H (endo H) treatment would specifically remove glycans from the dog and cat TfRs, we incubated 13.5 μg of FPLC-purified, denatured TfR with 4,000 U of endo Hf (New England BioLabs, Ipswich, MA) overnight according to the manufacturer's protocols and then analyzed the electrophoretic mobility by SDS-PAGE. ..

    SDS Page:

    Article Title: Single Mutations in the VP2 300 Loop Region of the Three-Fold Spike of the Carnivore Parvovirus Capsid Can Determine Host Range
    Article Snippet: The relative quantitation of the sugar glycan isoforms of N-linked peptide ions was carried out based on precursor ion peak areas under the assumption that all sugar glycan isoforms linked to the same core peptide have identical or similar ionization efficiencies, as reported previously ( ). .. To test if endoglycosidase H (endo H) treatment would specifically remove glycans from the dog and cat TfRs, we incubated 13.5 μg of FPLC-purified, denatured TfR with 4,000 U of endo Hf (New England BioLabs, Ipswich, MA) overnight according to the manufacturer's protocols and then analyzed the electrophoretic mobility by SDS-PAGE. ..

    other:

    Article Title: Two Modes of Regulation of the Fatty Acid Elongase ELOVL6 by the 3-Ketoacyl-CoA Reductase KAR in the Fatty Acid Elongation Cycle
    Article Snippet: Deglycosylation of Proteins Endoglycosidase H (Endo H) and peptide: N -glycosidase F (PNGase F) were purchased from New England Biolabs (Beverly, MA).

    Article Title: p97-dependent retrotranslocation and proteolytic processing govern formation of active Nrf1 upon proteasome inhibition
    Article Snippet: These cell lysates were used for deglycosylation reactions with the enzyme Endoglycosidase H for 1 hr at 37°C as per the manufacturer’s recommendations (New England Biolabs, Ipswich, MA).

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  • 94
    New England Biolabs α n acetylgalactosaminidase
    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N <t>-acetylgalactosaminidase</t> treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red
    α N Acetylgalactosaminidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α n acetylgalactosaminidase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α n acetylgalactosaminidase - by Bioz Stars, 2021-05
    94/100 stars
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    86
    New England Biolabs o glycanase
    New workflow for IgA1 O -glycomic analysis by using the sequential deglycosylation protocol. ( a ) Protocol for the profiling of IgA1 HR O -glycans. Neuraminidase treatment reduces the types of O -glycan structures from six to two (disaccharide N -acetylgalactosamine [GalNAc]-galactose [Gal] and monosaccharide GalNAc). LC-MS spectrum contains several mass peaks of HR with O -glycans, according to the number of the attached GalNAc and Gal residues. An extracted ion chromatogram (XIC) is generated for each peak and the area under the curve (AUC) of XIC of each O -glycopeptide is determined and used to calculate the relative abundance (RA) of each O -glycoform (RA = percent of total XIC of O -glycoforms detected). ( b ) Protocol for the analysis of the attachment sites of galactose-deficient (Gd) O -glycan. IgA1 proteins were treated with an IgA-specific protease from Clostridium ramosum AK183. After a sequential enzymatic deglycosylation with neuraminidase and O <t>-glycanase,</t> that ultimately leaves only Gd O -glycans ( i.e ., GalNAc) attached to the amino-acid backbone, IgA1 is digested by trypsin. Precursor ions corresponding to HR with one to three Gd O -glycans are analyzed by LC-tandem MS with ETD combined with supplemental higher energy collision dissociation (HCD) activation (EThcD) to assign the attachment sites of Gd O -glycans.
    O Glycanase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/o glycanase/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    o glycanase - by Bioz Stars, 2021-05
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    Image Search Results


    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Journal: The Journal of Biological Chemistry

    Article Title: The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface *

    doi: 10.1074/jbc.M113.476531

    Figure Lengend Snippet: Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Article Snippet: PNGase F (from Flavobacterium meningosepticum ), O -glycosidase (cloned from Enterococcus faecalis and expressed in Escherichia coli ), α- N -acetylgalactosaminidase (cloned from Chryseobacterium meningosepticum and expressed in E. coli ), and β1,3-galactosidase (cloned from Xanthomonas manihotis and expressed in E. coli ) were obtained from New England Biolabs (Ipswich, MA).

    Techniques: Binding Assay

    ( A ) EIC of m/z 548.2 from the BMO pool showing two main isomers. ( B ) EIC of m/z 548.2 after digestion with α N -acetylgalactosaminidase. ( C ) EIC of m/z 548.2 after digestion with β N -acetylhexosaminidase. ( D ) Magnification on base line

    Journal: Glycobiology

    Article Title: Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures

    doi: 10.1093/glycob/cwt007

    Figure Lengend Snippet: ( A ) EIC of m/z 548.2 from the BMO pool showing two main isomers. ( B ) EIC of m/z 548.2 after digestion with α N -acetylgalactosaminidase. ( C ) EIC of m/z 548.2 after digestion with β N -acetylhexosaminidase. ( D ) Magnification on base line

    Article Snippet: Recombinant β1-3,6 galactosidase, α1-3,6 galactosidase, β N -acetylhexosaminidase, α N -acetylgalactosaminidase, β N -acetylhexosaminidase, β N -acetylglucosaminidase and α2-3 neuraminidase were purchased from New England Biolabs (Ispwich, MA). β1-4 galactosidase was purchased from Prozyme (Hayward, CA).

    Techniques:

    New workflow for IgA1 O -glycomic analysis by using the sequential deglycosylation protocol. ( a ) Protocol for the profiling of IgA1 HR O -glycans. Neuraminidase treatment reduces the types of O -glycan structures from six to two (disaccharide N -acetylgalactosamine [GalNAc]-galactose [Gal] and monosaccharide GalNAc). LC-MS spectrum contains several mass peaks of HR with O -glycans, according to the number of the attached GalNAc and Gal residues. An extracted ion chromatogram (XIC) is generated for each peak and the area under the curve (AUC) of XIC of each O -glycopeptide is determined and used to calculate the relative abundance (RA) of each O -glycoform (RA = percent of total XIC of O -glycoforms detected). ( b ) Protocol for the analysis of the attachment sites of galactose-deficient (Gd) O -glycan. IgA1 proteins were treated with an IgA-specific protease from Clostridium ramosum AK183. After a sequential enzymatic deglycosylation with neuraminidase and O -glycanase, that ultimately leaves only Gd O -glycans ( i.e ., GalNAc) attached to the amino-acid backbone, IgA1 is digested by trypsin. Precursor ions corresponding to HR with one to three Gd O -glycans are analyzed by LC-tandem MS with ETD combined with supplemental higher energy collision dissociation (HCD) activation (EThcD) to assign the attachment sites of Gd O -glycans.

    Journal: Scientific Reports

    Article Title: Analysis of O-glycoforms of the IgA1 hinge region by sequential deglycosylation

    doi: 10.1038/s41598-020-57510-z

    Figure Lengend Snippet: New workflow for IgA1 O -glycomic analysis by using the sequential deglycosylation protocol. ( a ) Protocol for the profiling of IgA1 HR O -glycans. Neuraminidase treatment reduces the types of O -glycan structures from six to two (disaccharide N -acetylgalactosamine [GalNAc]-galactose [Gal] and monosaccharide GalNAc). LC-MS spectrum contains several mass peaks of HR with O -glycans, according to the number of the attached GalNAc and Gal residues. An extracted ion chromatogram (XIC) is generated for each peak and the area under the curve (AUC) of XIC of each O -glycopeptide is determined and used to calculate the relative abundance (RA) of each O -glycoform (RA = percent of total XIC of O -glycoforms detected). ( b ) Protocol for the analysis of the attachment sites of galactose-deficient (Gd) O -glycan. IgA1 proteins were treated with an IgA-specific protease from Clostridium ramosum AK183. After a sequential enzymatic deglycosylation with neuraminidase and O -glycanase, that ultimately leaves only Gd O -glycans ( i.e ., GalNAc) attached to the amino-acid backbone, IgA1 is digested by trypsin. Precursor ions corresponding to HR with one to three Gd O -glycans are analyzed by LC-tandem MS with ETD combined with supplemental higher energy collision dissociation (HCD) activation (EThcD) to assign the attachment sites of Gd O -glycans.

    Article Snippet: To remove disaccharides from HR glycopeptides, the digests were treated overnight with 40,000 U O -glycanase from Enterococcus faecalis (P0733, New England BioLabs, Ipswich, MA, USA) at 37 °C.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Generated, Activation Assay

    Mass spectrum and extracted ion chromatogram (XIC) of IgA1 HR peptide and glycopeptides with Gd O -glycans after sequential deglycosylation. Sequential neuraminidase and O -glycanase treatment leaves only Gd O -glycan(s) at V 222 –R 245 HR. The respective peaks correspond to naked V 222 –R 245 HR and V 222 –R 245 HR with one, two, or three Gd O -glycans. The number of GalNAc residues attached to HR is designated by the number after yellow squares. The theoretical monoisotopic mass values of “naked” V 222 –R 245 HR peptide and V 222 –R 245 HR glycopeptide with one to three Gd O -glycans are shown beside the peaks. The XIC for each Gd O -glycoform is shown next to the respective ion peak.

    Journal: Scientific Reports

    Article Title: Analysis of O-glycoforms of the IgA1 hinge region by sequential deglycosylation

    doi: 10.1038/s41598-020-57510-z

    Figure Lengend Snippet: Mass spectrum and extracted ion chromatogram (XIC) of IgA1 HR peptide and glycopeptides with Gd O -glycans after sequential deglycosylation. Sequential neuraminidase and O -glycanase treatment leaves only Gd O -glycan(s) at V 222 –R 245 HR. The respective peaks correspond to naked V 222 –R 245 HR and V 222 –R 245 HR with one, two, or three Gd O -glycans. The number of GalNAc residues attached to HR is designated by the number after yellow squares. The theoretical monoisotopic mass values of “naked” V 222 –R 245 HR peptide and V 222 –R 245 HR glycopeptide with one to three Gd O -glycans are shown beside the peaks. The XIC for each Gd O -glycoform is shown next to the respective ion peak.

    Article Snippet: To remove disaccharides from HR glycopeptides, the digests were treated overnight with 40,000 U O -glycanase from Enterococcus faecalis (P0733, New England BioLabs, Ipswich, MA, USA) at 37 °C.

    Techniques:

    A: Solubilized OMC45 polypeptide (lane 1) was treated with N-glycanase (lane 2) and O-glycanase (lane 3) separately following the manufacturer’s instructions. After enzymatic digestion, deglycosylated OMC45 polypeptide was analzed by Western blot

    Journal: Molecular and cellular biochemistry

    Article Title: Identification and Characterization of a Bovine Sperm Acrosomal Matrix Protein and its Mechanism of Interaction with Acrosomal Hydrolases

    doi: 10.1007/s11010-015-2534-8

    Figure Lengend Snippet: A: Solubilized OMC45 polypeptide (lane 1) was treated with N-glycanase (lane 2) and O-glycanase (lane 3) separately following the manufacturer’s instructions. After enzymatic digestion, deglycosylated OMC45 polypeptide was analzed by Western blot

    Article Snippet: Both N-glycanase and O-glycanase were purchased from New England Biolabs., MA.

    Techniques: Western Blot