Journal: Molecular Medicine Reports

Article Title: Sodium arsenite and dimethylarsenic acid induces apoptosis in OC3 oral cavity cancer cells

doi: 10.3892/mmr.2022.12913

Figure Lengend Snippet: Sodium arsenite upregulates cleaved caspase-9, −8, −3 and PARP protein expressions in OC3 cells. OC3 cells were incubated with 0, 10 and 25 µM NaAsO 2 for 3, 6, 12 and 24 h, respectively. (A) Expression of cleaved caspase-9 (35/37 kDa), −8 (43 kDa), −3 (17/19 kDa) and PARP (85~90 kDa) were examine via western blotting. Integrated optical intensities of (B) cleaved caspase-8, (C) −9, (D) −3 and (E) PARP were standardized by β-actin (43 kDa) among lanes. Results are demonstrated as mean ± standard error of the mean of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001 vs. control. Ctrl, control.

Article Snippet: Antibodies against β-actin (cat. no. 58169; 1:5,000), JNK (cat. no. 9252), phosphorylated (p)-JNK (cat. no. 9251), ERK1/2 (cat. no. 9102), p-ERK1/2 (cat. no. 9101), p38 (cat. no. 9212), p-p38 (cat. no. 9215), cleaved caspase-8 (cat. no. 9429), cleaved PARP (cat. no. 9542), cleaved caspase-9 (cat. no. 9509) and cleaved caspase-3 (cat. no. 9661) were attained from Cell Signaling Technology, Inc. Donkey anti-rabbit IgG (cat. no. NEF81200-1EA) conjugated to HRP was bought from PerkinElmer, Inc. ECL detection kit was obtained from MilliporeSigma.

Techniques: Incubation, Expressing, Western Blot

Journal: Molecular Medicine Reports

Article Title: Sodium arsenite and dimethylarsenic acid induces apoptosis in OC3 oral cavity cancer cells

doi: 10.3892/mmr.2022.12913

Figure Lengend Snippet: Dimethylarsenic acid upregulates cleaved caspase-9, −8, −3 and PARP protein expressions in OC3 cells. OC3 cells were incubated with 0, 1 and 10 mM DMA for 3, 6, 12 and 24 h. (A) Expression of cleaved caspase-9 (35/37 kDa), −8 (43 kDa), −3 (17/19 kDa) and PARP (85~90 kDa) were examine via western blotting (A) Integrated optical intensities (B) cleaved caspase-8, (C) −9, (D) −3 and (E) PARP were standardized by β-actin (43 kDa) among lanes. Results are demonstrated as mean ± standard error of the mean of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001 vs. control. DMA, dimethylarsenic acid; C/Ctrl, control.

Article Snippet: Antibodies against β-actin (cat. no. 58169; 1:5,000), JNK (cat. no. 9252), phosphorylated (p)-JNK (cat. no. 9251), ERK1/2 (cat. no. 9102), p-ERK1/2 (cat. no. 9101), p38 (cat. no. 9212), p-p38 (cat. no. 9215), cleaved caspase-8 (cat. no. 9429), cleaved PARP (cat. no. 9542), cleaved caspase-9 (cat. no. 9509) and cleaved caspase-3 (cat. no. 9661) were attained from Cell Signaling Technology, Inc. Donkey anti-rabbit IgG (cat. no. NEF81200-1EA) conjugated to HRP was bought from PerkinElmer, Inc. ECL detection kit was obtained from MilliporeSigma.

Techniques: Incubation, Expressing, Western Blot

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Maresin-1 Inhibits Oxidative Stress and Inflammation and Promotes Apoptosis in a Mouse Model of Caerulein-Induced Acute Pancreatitis

doi: 10.12659/MSM.917380

Figure Lengend Snippet: Mice with caerulein-induced acute pancreatitis (AP) treated with maresin-1 (MaR1) showed increased expression of apoptosis-associated proteins. Western blot shows the expression levels of Bcl-2, Bax, cleaved caspase-3 and cleaved caspase-9. *** P<0.001 vs. control; ## P<0.01, ### P<0.001 vs. AP. MaR1 – maresin-1; AP – acute pancreatitis

Article Snippet: The primary antibodies were obtained from Cell Signaling Technology (Boston, MA, USA) and included anti-Bcl-2 (3498S), anti-Bax (14796S), anti-cleaved caspase-3 (9664S), anti-cleaved caspase-9 (9509T), anti-TLR4 (14358S), anti-MyD88 (4283S), anti-phospho-NF-κB p65 (3033S), anti-NF-κB p65 (8242S), and anti-GAPDH (5174S).

Techniques: Expressing, Western Blot