bad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bad
    Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bad/product/Cell Signaling Technology Inc
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    bad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bad
    Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bad/product/Cell Signaling Technology Inc
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    bad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bad
    JNK activation is required for spautin-1-induced <t>BAD</t> expression. (A) Western blot analysis of expression of indicated proteins in HCT116 and CT26 cells following treatment with spautin-1 (10 uM) for 24 hours. (B, C) Indicated cells were treated with spautin-1 (10 uM) in the absence or presence of SP600125 (500 nM), CC-401 (100 nM), SB203580 (1 uM), SB239063 (100 nM), SCH772984 (100 nM), and LY3214996 (50 nM) for 24 hours. Bad mRNA and cell viability were assayed (n = 3, *p < 0.05, unpaired t-test). (D) Western blot analysis of expression of indicated proteins in HCT116 and CT26 cells following treatment with spautin-1 (10 uM) in the absence or presence of SP600125 (500 nM) or CC-401 (100 nM) for 24 hours. (E) Q-PCR analysis of Jun gene expression in indicated Jun knockdown cancer cells (n = 3, *p < 0.05 versus control shRNA group, unpaired t-test). (F-I) Knockdown of Jun inhibited spautin-1-(10µM) induced Bad mRNA expression (F), cell death (G), Bad promoter activity (H), <t>and</t> <t>CASP3</t> activity (I) in indicated cells (n = 3, *p < 0.05 versus control shRNA group, unpaired t-test).
    Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "TFAM is a novel mediator of immunogenic cancer cell death"

    Article Title: TFAM is a novel mediator of immunogenic cancer cell death

    Journal: Oncoimmunology

    doi: 10.1080/2162402X.2018.1431086

    JNK activation is required for spautin-1-induced BAD expression. (A) Western blot analysis of expression of indicated proteins in HCT116 and CT26 cells following treatment with spautin-1 (10 uM) for 24 hours. (B, C) Indicated cells were treated with spautin-1 (10 uM) in the absence or presence of SP600125 (500 nM), CC-401 (100 nM), SB203580 (1 uM), SB239063 (100 nM), SCH772984 (100 nM), and LY3214996 (50 nM) for 24 hours. Bad mRNA and cell viability were assayed (n = 3, *p < 0.05, unpaired t-test). (D) Western blot analysis of expression of indicated proteins in HCT116 and CT26 cells following treatment with spautin-1 (10 uM) in the absence or presence of SP600125 (500 nM) or CC-401 (100 nM) for 24 hours. (E) Q-PCR analysis of Jun gene expression in indicated Jun knockdown cancer cells (n = 3, *p < 0.05 versus control shRNA group, unpaired t-test). (F-I) Knockdown of Jun inhibited spautin-1-(10µM) induced Bad mRNA expression (F), cell death (G), Bad promoter activity (H), and CASP3 activity (I) in indicated cells (n = 3, *p < 0.05 versus control shRNA group, unpaired t-test).
    Figure Legend Snippet: JNK activation is required for spautin-1-induced BAD expression. (A) Western blot analysis of expression of indicated proteins in HCT116 and CT26 cells following treatment with spautin-1 (10 uM) for 24 hours. (B, C) Indicated cells were treated with spautin-1 (10 uM) in the absence or presence of SP600125 (500 nM), CC-401 (100 nM), SB203580 (1 uM), SB239063 (100 nM), SCH772984 (100 nM), and LY3214996 (50 nM) for 24 hours. Bad mRNA and cell viability were assayed (n = 3, *p < 0.05, unpaired t-test). (D) Western blot analysis of expression of indicated proteins in HCT116 and CT26 cells following treatment with spautin-1 (10 uM) in the absence or presence of SP600125 (500 nM) or CC-401 (100 nM) for 24 hours. (E) Q-PCR analysis of Jun gene expression in indicated Jun knockdown cancer cells (n = 3, *p < 0.05 versus control shRNA group, unpaired t-test). (F-I) Knockdown of Jun inhibited spautin-1-(10µM) induced Bad mRNA expression (F), cell death (G), Bad promoter activity (H), and CASP3 activity (I) in indicated cells (n = 3, *p < 0.05 versus control shRNA group, unpaired t-test).

    Techniques Used: Activation Assay, Expressing, Western Blot, shRNA, Activity Assay

    rabbit anti bad  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc rabbit anti bad
    Rabbit Anti Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bad
    Effects of BSJPD-medicated serum, licochalcone A, alisol B, and hederagenin on the expression of PI3K-Akt-mTOR pathway components and apoptosis-related pathway molecules in HepG2 cells. (a) Western blot analysis of the effects of BSJPD-medicated serum on the expression of PI3K, p-Akt, Akt, p-mTOR, <t>mTOR,</t> <t>Bcl-xL,</t> <t>BAD,</t> p53, and CASP3. (b) Western blot analysis of the effects of licochalcone A, alisol B, and hederagenin on the expression of PI3K, p-Akt, Akt, p-mTOR, mTOR, Bcl-xL, BAD, p53, and CASP3. Notes. BSJPD, Bushen-Jianpi decoction; LCLA, licochalcone A; ALSB, alisol B; HDG, hederagenin; control, cultured with FBS; rat control, cultured with rat serum. ∗ p < 0.05 and ∗∗ p < 0.01 compared with the control group. These groupings of blots are cropped from the same gel or different gels. All full-length blots are included in Supplementary Figures and .
    Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Network Pharmacology-Based Study on the Mechanism of Bushen-Jianpi Decoction in Liver Cancer Treatment"

    Article Title: Network Pharmacology-Based Study on the Mechanism of Bushen-Jianpi Decoction in Liver Cancer Treatment

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2019/3242989

    Effects of BSJPD-medicated serum, licochalcone A, alisol B, and hederagenin on the expression of PI3K-Akt-mTOR pathway components and apoptosis-related pathway molecules in HepG2 cells. (a) Western blot analysis of the effects of BSJPD-medicated serum on the expression of PI3K, p-Akt, Akt, p-mTOR, mTOR, Bcl-xL, BAD, p53, and CASP3. (b) Western blot analysis of the effects of licochalcone A, alisol B, and hederagenin on the expression of PI3K, p-Akt, Akt, p-mTOR, mTOR, Bcl-xL, BAD, p53, and CASP3. Notes. BSJPD, Bushen-Jianpi decoction; LCLA, licochalcone A; ALSB, alisol B; HDG, hederagenin; control, cultured with FBS; rat control, cultured with rat serum. ∗ p < 0.05 and ∗∗ p < 0.01 compared with the control group. These groupings of blots are cropped from the same gel or different gels. All full-length blots are included in Supplementary Figures and .
    Figure Legend Snippet: Effects of BSJPD-medicated serum, licochalcone A, alisol B, and hederagenin on the expression of PI3K-Akt-mTOR pathway components and apoptosis-related pathway molecules in HepG2 cells. (a) Western blot analysis of the effects of BSJPD-medicated serum on the expression of PI3K, p-Akt, Akt, p-mTOR, mTOR, Bcl-xL, BAD, p53, and CASP3. (b) Western blot analysis of the effects of licochalcone A, alisol B, and hederagenin on the expression of PI3K, p-Akt, Akt, p-mTOR, mTOR, Bcl-xL, BAD, p53, and CASP3. Notes. BSJPD, Bushen-Jianpi decoction; LCLA, licochalcone A; ALSB, alisol B; HDG, hederagenin; control, cultured with FBS; rat control, cultured with rat serum. ∗ p < 0.05 and ∗∗ p < 0.01 compared with the control group. These groupings of blots are cropped from the same gel or different gels. All full-length blots are included in Supplementary Figures and .

    Techniques Used: Expressing, Western Blot, Cell Culture

    bad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bad
    Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bad/product/Cell Signaling Technology Inc
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    bad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bad
    Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bad/product/Cell Signaling Technology Inc
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    rabbit anti bad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti bad
    Changes of apoptosis-related proteins between young and adult rats after IR. Western blot analysis of apoptosis-related proteins ( A <t>);</t> <t>Caspase-8</t> expression ( B ); Cleaved caspase-8 expression ( C ); Caspase-3 expression ( D ); Cleaved caspase-3expression ( E ); <t>Bad</t> expression ( F ), Bcl-xL expression ( G ) and Bcl-2 expression ( H ). (n = 7 per group; * p < 0.05, significantly different from the respectively sham-group; # p < 0.05, significantly different from the corresponding young group; $ p < 0.05, significantly different from the corresponding 1 day group; full-length blots are presented in Additional file : Fig. S2). The bars indicate the means ± SEM
    Rabbit Anti Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Enhanced autophagy interacting proteins negatively correlated with the activation of apoptosis-related caspase family proteins after focal ischemic stroke of young rats"

    Article Title: Enhanced autophagy interacting proteins negatively correlated with the activation of apoptosis-related caspase family proteins after focal ischemic stroke of young rats

    Journal: BMC Neuroscience

    doi: 10.1186/s12868-022-00740-w

    Changes of apoptosis-related proteins between young and adult rats after IR. Western blot analysis of apoptosis-related proteins ( A ); Caspase-8 expression ( B ); Cleaved caspase-8 expression ( C ); Caspase-3 expression ( D ); Cleaved caspase-3expression ( E ); Bad expression ( F ), Bcl-xL expression ( G ) and Bcl-2 expression ( H ). (n = 7 per group; * p < 0.05, significantly different from the respectively sham-group; # p < 0.05, significantly different from the corresponding young group; $ p < 0.05, significantly different from the corresponding 1 day group; full-length blots are presented in Additional file : Fig. S2). The bars indicate the means ± SEM
    Figure Legend Snippet: Changes of apoptosis-related proteins between young and adult rats after IR. Western blot analysis of apoptosis-related proteins ( A ); Caspase-8 expression ( B ); Cleaved caspase-8 expression ( C ); Caspase-3 expression ( D ); Cleaved caspase-3expression ( E ); Bad expression ( F ), Bcl-xL expression ( G ) and Bcl-2 expression ( H ). (n = 7 per group; * p < 0.05, significantly different from the respectively sham-group; # p < 0.05, significantly different from the corresponding young group; $ p < 0.05, significantly different from the corresponding 1 day group; full-length blots are presented in Additional file : Fig. S2). The bars indicate the means ± SEM

    Techniques Used: Western Blot, Expressing

    bad mab rabbit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bad mab rabbit

    Bad Mab Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Sensitization of FOLFOX-resistant colorectal cancer cells via the modulation of a novel pathway involving protein phosphatase 2A"

    Article Title: Sensitization of FOLFOX-resistant colorectal cancer cells via the modulation of a novel pathway involving protein phosphatase 2A

    Journal: iScience

    doi: 10.1016/j.isci.2022.104518


    Figure Legend Snippet:

    Techniques Used: Recombinant, Purification, Bicinchoninic Acid Protein Assay, Expressing, Software

    bad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bad
    Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bad/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    bad  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc bad
    Bad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Journal: iScience

    Article Title: Sensitization of FOLFOX-resistant colorectal cancer cells via the modulation of a novel pathway involving protein phosphatase 2A

    doi: 10.1016/j.isci.2022.104518

    Figure Lengend Snippet:

    Article Snippet: BAD mAb (Rabbit) , Cell Signaling Technology , Cat# 9239; RRID:AB_2062127.

    Techniques: Recombinant, Purification, Bicinchoninic Acid Protein Assay, Expressing, Software