Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: Primers Used in qPCR

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Sequencing

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: Primers Used in Chromatin Isolation by RNA Precipitation (CHIRP)

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Isolation, Sequencing

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: BCAR4 regulated the downstream proteins of GLI2. Results of chromatin isolation by RNA precipitation (CHIRP) assay showed that levels of the GLI2 downstream proteins (A) RPS3, (B) IL-6, (C) MUC5AC, and (D) TGF-β1 were dramatically higher in the BCAR4 group. * p < 0.05, compared with the LacZ [negative control (NC)] group.

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Isolation, Negative Control

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: BCAR4 regulated GLI2 expression. The mRNA expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group of (A) A549 and (B) HCC827 cells. * p < 0.05, ** p < 0.01, compared with the NC group. (C–F) In both cell lines, the protein expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group. * p < 0.05, ** p < 0.01, compared with the NC group.

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Expressing

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: GLI2 downstream protein expression was positively correlated with BCAR4 expression. (A) Results of the qRT-PCR showed that the expression levels of RPS3, IL-6, MUC5AC, and TGF-β1 were much higher in NSCLC tissues than in adjacent tissues. * p < 0.05, compared with adjacent tissues. The expression levels of (B) RPS3, (C) IL-6, (D) MUC5AC, and (E) TGF-β1 were positively correlated with BCAR4 expression.

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: Synergistic Interactions between Cytokines and AVP at the Blood-CSF Barrier Result in Increased Chemokine Production and Augmented Influx of Leukocytes after Brain Injury

doi: 10.1371/journal.pone.0079328

Figure Lengend Snippet: The activation of the JNK signaling cascade in the Z310 cell line was assessed after 1-h incubation with either TNF-α (5 ng/ml) or a combination of TNF-α (5 ng/ml) and AVP (1 nM). In non-radioactive JNK activity assays, 200 ng of recombinant (rec) human c-Jun protein was used as a substrate for JNK. The extent of activation of c-Jun and ATF2 was assessed by Western blotting (30 µg of total protein per lane was loaded). The representative immunoblots are shown based on three independent experiments. The ratios of optical density of bands for phosphorylated proteins over the optical density of bands for the total (phosphorylated and non-phosphorylated) proteins are shown.

Article Snippet: The rabbit monoclonal antibodies were as follows: anti-human c-Jun (clone 60A8; diluted 1∶200) and anti-human activating transcription factor 2 (ATF2) (clone 20F1; diluted 1∶200) both from Cell Signaling.

Techniques: Activation Assay, Incubation, Activity Assay, Recombinant, Western Blot

Journal: PLoS ONE

Article Title: Synergistic Interactions between Cytokines and AVP at the Blood-CSF Barrier Result in Increased Chemokine Production and Augmented Influx of Leukocytes after Brain Injury

doi: 10.1371/journal.pone.0079328

Figure Lengend Snippet: To inhibit the activity of JNK in rats sustaining TBI, a selective JNK inhibitor SP600125 (JNK Inh) was injected i.p. at 4 and 8 h post-TBI at a dose of 30 mg/kg for each injection. A control group of rats was injected with vehicle (Veh; DMSO). (A) The efficacy of pharmacological inhibition of JNK as assessed by the reduction in post-traumatic activation of ATF2 in the lateral ventricle choroid plexus (CP) at 6 h after injury. Another target transcription factor for JNK, c-Jun, was undetectable in the CP. The activity of ATF2 was assessed by Western blotting in both the ipsilateral (Ipsi CP/I) and contralateral (Contr CP/C) CPs (30 µg of total protein per lane was loaded). The representative immunoblots are shown based on two independent experiments involving separate groups of animals. The ratios of optical density of bands for phosphorylated ATF2 over the optical density of bands for the total (phosphorylated and non-phosphorylated) ATF2 are shown. (B) The effect of JNK inhibition on post-traumatic production of neutrophil chemoattractants CXCL1–3 by the lateral ventricle CP as assessed by real-time RT-PCR at 6 h after injury. Cyclophilin A (Cycl-A) was used for the normalization of the data. (C) The role of AVP in promoting the post-traumatic influx of neutrophils across the blood-cerebrospinal fluid barrier (BCSFB) and the effect of JNK inhibition on this influx. To define the pathophysiological role of AVP in promoting neutrophil influx across the BCSFB, AVP-deficient Brattleboro rats ( Avp di/di ) were compared with their parental Long-Evans strain (WT). The magnitude of neutrophil influx was assessed by the myeloperoxidase (MPO) activity assays at 24 h post-TBI. * p <0.05 for JNK Inh versus Veh or for Avp di/di versus WT ( n = 4–6 per group).

Article Snippet: The rabbit monoclonal antibodies were as follows: anti-human c-Jun (clone 60A8; diluted 1∶200) and anti-human activating transcription factor 2 (ATF2) (clone 20F1; diluted 1∶200) both from Cell Signaling.

Techniques: Activity Assay, Injection, Inhibition, Activation Assay, Western Blot, Quantitative RT-PCR

Journal: Frontiers in Immunology

Article Title: A Novel Bispecific Antibody Targeting PD-L1 and VEGF With Combined Anti-Tumor Activities

doi: 10.3389/fimmu.2021.778978

Figure Lengend Snippet: The information of primary and secondary antibodies used in mIHC detection.

Article Snippet: mouse anti-human CD8α antibody (1:100 dilution) (70306s, Cell Signaling Technology) , Anti-mouse IgG (H+L), F(ab’)2Fragment (Alexa Fluor ® 488 Conjugate) (Cell Signaling Technology).

Techniques:

Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet:

Article Snippet: Anti-human/mouse pIKKa/b [S176/180] clone 16A6 , Cell Signaling Technology , 2697.

Techniques: Blocking Assay, Recombinant, Plasmid Preparation, Lysis, Staining, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Sequencing, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry

Journal: Inflammopharmacology

Article Title: Boswellic acids/ Boswellia serrata extract as a potential COVID-19 therapeutic agent in the elderly

doi: 10.1007/s10787-021-00841-8

Figure Lengend Snippet: Immunomodulatory effects of Boswellia extract and boswellic acids in articles published from 1991 to 2020

Article Snippet: B. carterii extract and 3- O -acetyl-alpha-boswellic acid , Cultured cells, lymphocytes were isolated from the blood of healthy adult donors obtained from a blood transfusion center , Inhibited proliferation, degranulation capacity, and secretion of inflammatory mediators of physiologically relevant anti-CD3 and anti-CD28 activated human T lymphocytes in a non-toxic concentration range , Immunosuppressive effects of the extract are based on specific NFAT-conditioned suppression within T cell signaling , Zimmermann-Klemd et al. ( ) .

Techniques: Concentration Assay, Inhibition, Activity Assay, In Vivo, Mouse Assay, Expressing, Sedimentation, Isolation, Transformation Assay, Serial Time-encoded Amplified Microscopy, Distillation, In Vitro, Luciferase, Reporter Assay, Cell Culture, Activation Assay, Ligation, Proliferation Assay

Journal: Cell metabolism

Article Title: Aerobic Glycolysis Controls Myeloid-Derived Suppressor Cells and Tumor Immunity via a Specific CEBPB Isoform in Triple-Negative Breast Cancer

doi: 10.1016/j.cmet.2018.04.022

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-human/mouse p-AMPKα , Cell Signaling Technology , Cat# 2535S; RRID: AB_331250.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Lactate Assay, Cell Isolation, Expressing, shRNA, Plasmid Preparation, Software