Journal: Behavioural Neurology

Article Title: Curcumin Relieves Chronic Unpredictable Mild Stress-Induced Depression-Like Behavior through the PGC-1 α /FNDC5/BDNF Pathway

doi: 10.1155/2021/2630445

Figure Lengend Snippet: Primer sequences used for the qPCR analysis.

Article Snippet: The following antibodies and concentrations were used overnight at a temperature of 4°C: PGC-1 α (ab106814, 1 : 1000, Abcam), estrogen-related receptor alpha (ERR α , #13826, 1 : 1000, Cell Signaling Technology), FNDC5 (23995-1-AP, 1 : 1000; Proteintech), BDNF (28205-1-AP 1 : 1000; Proteintech), and β -actin (8046S, 1 : 1000, Cell Signaling Technology).

Techniques: Amplification

Journal: Behavioural Neurology

Article Title: Curcumin Relieves Chronic Unpredictable Mild Stress-Induced Depression-Like Behavior through the PGC-1 α /FNDC5/BDNF Pathway

doi: 10.1155/2021/2630445

Figure Lengend Snippet: (a) and (b) Western blot bands indicating the protein expressions of PGC-1 α and ERR α in the hippocampus. (c)–(f) Protein expressions of PGC-1 α and ERR α normalized to that of the β -actin internal control. Data are expressed as means ± SD ( n = 7). ∗ p < 0.05 and ∗∗ p < 0.01 compared to the control group. + p < 0.05 and ++ p < 0.01 compared to the CUMS group. # p < 0.05 and ## p < 0.01 compared to the CUMS + CUR group.

Article Snippet: The following antibodies and concentrations were used overnight at a temperature of 4°C: PGC-1 α (ab106814, 1 : 1000, Abcam), estrogen-related receptor alpha (ERR α , #13826, 1 : 1000, Cell Signaling Technology), FNDC5 (23995-1-AP, 1 : 1000; Proteintech), BDNF (28205-1-AP 1 : 1000; Proteintech), and β -actin (8046S, 1 : 1000, Cell Signaling Technology).

Techniques: Western Blot

Journal: Behavioural Neurology

Article Title: Curcumin Relieves Chronic Unpredictable Mild Stress-Induced Depression-Like Behavior through the PGC-1 α /FNDC5/BDNF Pathway

doi: 10.1155/2021/2630445

Figure Lengend Snippet: (a) Relative mRNA expressions of FNDC5 and BDNF. (b) Western blot bands indicating the protein expressions of FNDC5 and BDNF. (c) and (d) Protein expressions of FNDC5 and BDNF normalized to that of the β -actin internal control. Data are expressed as means ± SD ( n = 7). ∗ p < 0.05 and ∗∗ p < 0.01 compared to the control group. + p < 0.05 and ++ p < 0.01 compared to the CUMS group. # p < 0.05 and ## p < 0.01 compared to the CUMS + CUR group.

Article Snippet: The following antibodies and concentrations were used overnight at a temperature of 4°C: PGC-1 α (ab106814, 1 : 1000, Abcam), estrogen-related receptor alpha (ERR α , #13826, 1 : 1000, Cell Signaling Technology), FNDC5 (23995-1-AP, 1 : 1000; Proteintech), BDNF (28205-1-AP 1 : 1000; Proteintech), and β -actin (8046S, 1 : 1000, Cell Signaling Technology).

Techniques: Western Blot