anti idh1wt (Cell Signaling Technology Inc)


Structured Review
Anti Idh1wt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti idh1wt/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti idh1wt (Cell Signaling Technology Inc)


Structured Review
Anti Idh1wt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti idh1wt/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti idh1wt (Cell Signaling Technology Inc)


Structured Review
Anti Idh1wt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti idh1wt/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rabbit igg anti idh1 (Cell Signaling Technology Inc)


Structured Review

Rabbit Igg Anti Idh1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg anti idh1/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A high-throughput SAMDI-mass spectrometry assay for isocitrate dehydrogenase 1"
Article Title: A high-throughput SAMDI-mass spectrometry assay for isocitrate dehydrogenase 1
Journal: The Analyst
doi: 10.1039/d0an00174k

Figure Legend Snippet: IDH1 catalyzes the conversion of isocitrate to αKG.
Techniques Used:

Figure Legend Snippet: (a) Scheme for the preparation and analysis of recombinant IDH1 samples. (b) αKG concentrations measured in the 384-sample biochemical screen (7 samples did not produce either 12C or 13C αKG capture).
Techniques Used: Recombinant

Figure Legend Snippet: IDH1 expression level as determined with western blot and αKG concentration as measured with SAMDI-MS for untreated cells and cells treated with transfection agent, control sequence siRNA and IDH1-targeting siRNA. All data are expressed as mean ± standard deviation with n = 2 experimental replicates.
Techniques Used: Expressing, Western Blot, Concentration Assay, Transfection, Sequencing, Standard Deviation
rabbit igg anti idh1 (Cell Signaling Technology Inc)


Structured Review

Rabbit Igg Anti Idh1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg anti idh1/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A high-throughput SAMDI-mass spectrometry assay for isocitrate dehydrogenase 1 † "
Article Title: A high-throughput SAMDI-mass spectrometry assay for isocitrate dehydrogenase 1
Journal: The Analyst
doi: 10.1039/d0an00174k

Figure Legend Snippet: IDH1 catalyzes the conversion of isocitrate to αKG.
Techniques Used:

Figure Legend Snippet: (a) Scheme for the preparation and analysis of recombinant IDH1 samples. (b) αKG concentrations measured in the 384-sample biochemical screen (7 samples did not produce either 12C or 13C αKG capture).
Techniques Used: Recombinant

Figure Legend Snippet: IDH1 expression level as determined with western blot and αKG concentration as measured with SAMDI-MS for untreated cells and cells treated with transfection agent, control sequence siRNA and IDH1-targeting siRNA. All data are expressed as mean ± standard deviation with n = 2 experimental replicates.
Techniques Used: Expressing, Western Blot, Concentration Assay, Transfection, Sequencing, Standard Deviation
rabbit igg anti idh1 (Cell Signaling Technology Inc)


Structured Review
Rabbit Igg Anti Idh1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg anti idh1/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rabbit igg anti idh1 (Cell Signaling Technology Inc)


Structured Review
Rabbit Igg Anti Idh1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg anti idh1/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti idh1 (Cell Signaling Technology Inc)


Structured Review

Anti Idh1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti idh1/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation"
Article Title: Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation
Journal: Cell Metabolism
doi: 10.1016/j.cmet.2019.05.003

Figure Legend Snippet:
Techniques Used: Recombinant, Enzyme-linked Immunosorbent Assay, Construct, Plasmid Preparation, Sequencing, Clone Assay, Software
phycoerythrin pe conjugated anti idh1 (Cell Signaling Technology Inc)


Structured Review

Phycoerythrin Pe Conjugated Anti Idh1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phycoerythrin pe conjugated anti idh1/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Sensitive Quantitative Proteomics of Human Hematopoietic Stem and Progenitor Cells by Data-independent Acquisition Mass Spectrometry"
Article Title: Sensitive Quantitative Proteomics of Human Hematopoietic Stem and Progenitor Cells by Data-independent Acquisition Mass Spectrometry
Journal: Molecular & Cellular Proteomics : MCP
doi: 10.1074/mcp.TIR119.001431

Figure Legend Snippet: Proteome-transcriptome correlation in human hematopoietic stem and progenitor cell subpopulations. ( A ) Nonsupervised hierarchical clustering (Euclidean distance) heatmap of the intensity for the transcripts identified in HSCs/MPPs (referred to as HSCs), CMPs, GMPs, and MEPs (shades of red) isolated from five different HSPC donors (shades of blue). The transcript intensities are centered and scaled and depicted in color shades from red to blue. The transcripts with missing transcript intensity values in all samples were removed because they could not be handled by the clustering algorithm. Remaining missing transcript intensity values are shown in white. Clustering was observed mostly according to cell type, not according to donor. ( B ) GO enrichment analysis showed good alignment of protein and mRNA data. GSEA was performed for ranked mRNA and protein lists using GO processes from the Gene Ontology Consortium database as gene sets. Shown are normalized enrichment scores for the individual gene sets. Significantly up-regulated gene sets are marked in blue color; significantly downregulated gene sets are marked in red color. Significance was defined as FDR < 0.25, specific cell subpopulations were compared with the average of the remaining three cell types, and log2(fold change) was used as ranking criterion. Empty fields mean that no enrichment could be calculated. Abbreviations: MEGA, megakaryocyte; MAPK, mitogen-activated protein kinase; PI3K, phosphoinositide-3-kinase; PLC, phospholipase C. ( C ) Correlation between proteomics and transcriptomics data for HSCs/MPPs (referred to as HSCs), CMPs, GMPs, and MEPs. Dots are depicted in red when the FDR was below 0.01 both for protein and RNA data, orange when FDR < 0.01 for protein data, purple when FDR < 0.01 for RNA data, and gray when FDR ≥ 0.01 for both protein and RNA data. ( D ) Network analysis of significantly up-regulated proteins with concomitant significantly downregulated mRNA in HSCs/MPPs. Two clusters were especially prominent, including the snoRNPs and telomerase complex proteins GAR1, DKC1, NOP10, NHP2, and the quiescence-inducing NAD(P)H-producing proteins IDH1, IDH3A, and IDH3B. HSCs/MPPs were compared with the average of the other three subpopulations; cutoffs were set at FDR < 0.01 for protein and RNA data. Colors depict GO terms found enriched in B .
Techniques Used: Isolation
d2h1 (Cell Signaling Technology Inc)


Structured Review
D2h1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d2h1/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
idh1 d2h1 antibodies (Cell Signaling Technology Inc)


Structured Review

Idh1 D2h1 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/idh1 d2h1 antibodies/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Characterization of single microvesicles in plasma from glioblastoma patients"
Article Title: Characterization of single microvesicles in plasma from glioblastoma patients
Journal: Neuro-Oncology
doi: 10.1093/neuonc/noy187

Figure Legend Snippet: Marker expression in microvesicles obtained from GBM8. (A) Images of MV-like vesicles labeled for generic EV markers (CD81, TSG101, CD63, Alix, integrin beta 1, CD9, CD40, Arf6, VAMP-3) and glioblastoma markers (EGFR, EGFRvIII, EpCAM, IDH1-R132H) were quantified per single vesicle. Heat map of data is organized by high expression to low expression. Note the heterogeneity profile of each individual MV-like vesicle. (B) Waterfall plot displaying the percent of the total MV-like vesicles that are positive for a given marker. IDH1-R132H was not detected in any of the vesicles.
Techniques Used: Marker, Expressing, Labeling

Figure Legend Snippet: Summary of marker expression in MV-like fractions obtained from different specimens
Techniques Used: Marker, Expressing

Figure Legend Snippet: Global marker expression in human GB plasma samples. (A) Total MV-like vesicle numbers in the plasma from each patient were determined via nanoparticle tracking analysis (NTA). (B) The amount of TMV was determined by first accounting for host cell MV (using a cocktail of CD31, CD41, CD42β, CD45, and CD235a) then staining remaining vesicles for tumor markers (EGFR, EGFRvIII, EpCAM, and IDH1-R132H). TMV were present and detectable in all GB patients analyzed. (C) The relative contribution of TMV to the total MV-like vesicle pool was variable among GB patients. (D) The heterogeneity of EGFR, EGFRvIII, and EpCAM expression (IDH1-R132H was not detected in any of the EV observed) among the subpopulation of TMV for each patient was assessed.
Techniques Used: Marker, Expressing, Staining

Figure Legend Snippet: Patient demographics and clinical characteristics
Techniques Used: