Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Rubi Fructus ( Rubus coreanus ) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells

doi: 10.1155/2013/475386

Figure Lengend Snippet: The primer sequences used for real-time PCR.

Article Snippet: Anti-PPAR γ , anti-aP2, anti-adiponectin, anti-phospho LKB1, anti-phospho AMPK α anti-AMPK α , and anti-ACC antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Rubi Fructus ( Rubus coreanus ) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells

doi: 10.1155/2013/475386

Figure Lengend Snippet: Effect of RF on the expression of aP2, resistin, and adiponectin in 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RF (0, 10, 50, and 100 μ g/mL) for 6 days. (a) aP2, (b) resistin, and (c) adiponectin mRNA expression were evaluated by the quantitative real-time PCR. (d) aP2, resistin, and adiponectin protein levels were analyzed by Western blot analysis. GAPDH was used as internal controls. Data are expressed as means ± S.D. where P < 0.05 was considered a statistically significant difference from the differentiated control.

Article Snippet: Anti-PPAR γ , anti-aP2, anti-adiponectin, anti-phospho LKB1, anti-phospho AMPK α anti-AMPK α , and anti-ACC antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: bioRxiv

Article Title: Coordination Between Embryo Growth and Trophoblast Migration Upon Implantation Delineates Mouse Embryogenesis

doi: 10.1101/2022.06.13.495767

Figure Lengend Snippet: (A) Left, representative immunofluorescence image of the E4.75 uterus cross-section, simultaneously stained for Fibronectin (FN1, white), GATA4 (green), and nuclei (DNA, blue). Right, 4x zoom into the interface between trophectoderm (TE), uterine epithelium (E), and stroma (S) (outlined). Red arrowheads mark uterine ECM. (B) Representative immunofluorescence image of the E5.25 uterus cross-section, simultaneously stained for pan-Laminin (panLAM, white), GATA4 (green), and nuclei (DNA, blue). Measurements of E5.25 in utero embryo dimension across n = 12. (C) Schematic illustration of peri-implantation embryo culture. Embryos are recovered at E3.5, treated by Tyrode’s solution to remove Zona pellucida (ZP), and embedded into the crypts on the day of recovery (D0). ICM, red; TE, blue; IVC1 and ICV2 stand for “In Vitro Culture” medium 1 and 2, respectively. Inset, schematic illustration of the hydrogel composition. 8-arm Poly(ethylene glycol) (PEG, gray) molecules, crosslinked via metalloprotease-cleavable peptides (Peptide, green), and functionalized with RGDSPG peptide (Arg-Gly-Asp-Ser-Pro-Gly, ‘RGD’, red). (D) Representative immunofluorescence images of the embryos developed in utero until E4.5 and E5.25, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (E) Representative immunofluorescence images of the 3E-uterus embryos from day 2 and 3, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (F) Scatterplot showing numbers of epiblast (EPI) cells (x-axis) vs numbers of visceral endoderm (VE) cells (y-axis) that cover epiblast in the embryos developed in utero until E5.5 (E3.5 – E5.5) and the embryos developed by 3E-uterus until day 3 (D1 – 3). n = 5 (E3.5), n = 21 (E4.5), n = 28 (E4.75), n = 20 (E5.0), n = 20 (E5.25), n = 21 (E5.5), data from ; n = 20, two replicates pooled (D1), n = 13 of 28, three replicates pooled (D2), n = 12 of 26, three replicates pooled (D3). X/Y scale, log 10. Bottom right, the scheme of the quantified embryo region, EPI (magenta), VE (green). (G) From left to right, boxplots showing egg cylinder’s length, diameter, and the length-to-diameter ratio between embryos developed in utero until E5.25 and 3E-uterus embryos from day 3 (D3). n = 14 and 12, respectively. Data points, shown as black dots, correspond to individual embryos, midline marks the median, boxes indicate interquartile range. P-values were calculated using Student’s t-test and the Mann-Whitney U test. (H) Cell number-based correspondence between in utero and 3E-uterus embryo development. (I) Left, representative immunofluorescence image of the E4.75 uterus cross-section from the pregnant F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 2 (D2). Yellow arrowheads mark differentiated trophoblast cells. (J) Left, representative immunofluorescence image of the E5.25 uterus cross-section from the F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 3 (D3). Scale bars, 50 μm, 12.5 μm (4x zoom). White asterisks mark epiblast of the implanted embryos. See also Materials and Methods, .

Article Snippet: Primary antibodies against GATA4 biotinylated (R&D systems, AF2606), SOX2 (Cell Signaling, 23064), TFAP2C (Cell Signaling, #2320), CDX2 (Biogenex Laboratories, MU392AUC), PARD6B (Santa Cruz Biotechnology, sc-67393), pan-Laminin (Novus Biologicals, NB300-144SS), Collagen IV (Millipore, AB756P), Fibronectin (Proteintech, 15613-1-AP), ITGB1 (Millipore, MAB1997), GFP (chromotek, gb2AF488) were diluted at 1:200.

Techniques: Immunofluorescence, Staining, In Utero, Embryo Culture, In Vitro, MANN-WHITNEY, Immunostaining

Journal: eLife

Article Title: High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures

doi: 10.7554/eLife.59445

Figure Lengend Snippet:

Article Snippet: Antibody , anti-AP-2γ (Rabbit polyclonal) , Cell Signaling Technology , Cat# 2320, RRID: AB_2202287 , (1:800).

Techniques: Recombinant, Multiplex Assay, Software

Journal: Cell stem cell

Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

doi: 10.1016/j.stem.2018.12.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Primary antibodies are: K18 (1:800, R&D AF7619), K14 (1:800, BioLegend SIG-3476–100); AP-2γ (1:100, Cell Signaling 2320), p63 (1:100 Gene Tex GTX102425), ITGA6 (1:200, Millipore, MAB1378).

Techniques: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Clone Assay, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software

Journal: Cell stem cell

Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

doi: 10.1016/j.stem.2018.12.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: AP-2γ antibody for IF , Cell Signaling , Cat #2320S RRID:AB_10695101.

Techniques: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Clone Assay, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software

Journal: Cell stem cell

Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

doi: 10.1016/j.stem.2018.12.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: AP-2γ antibody for IF , Cell Signaling , Cat #2320S RRID:AB_10695101.

Techniques: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Clone Assay, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software