cd147  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd147
    Expression levels of key gastric cancer stem cell (GCSC) markers, CypA, and <t>CD147</t> in MKN45 adherent and tumorsphere cells. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for adherent cells was normalized to onefold. * p < 0.05 vs. the adherent cells.
    Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd147/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    cd147 - by Bioz Stars, 2024-06
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    Images

    1) Product Images from "Cyclophilin A Inhibitors Suppress Proliferation and Induce Apoptosis of MKN45 Gastric Cancer Stem-like Cells by Regulating CypA/CD147-Mediated Signaling Pathway"

    Article Title: Cyclophilin A Inhibitors Suppress Proliferation and Induce Apoptosis of MKN45 Gastric Cancer Stem-like Cells by Regulating CypA/CD147-Mediated Signaling Pathway

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms24054734

    Expression levels of key gastric cancer stem cell (GCSC) markers, CypA, and CD147 in MKN45 adherent and tumorsphere cells. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for adherent cells was normalized to onefold. * p < 0.05 vs. the adherent cells.
    Figure Legend Snippet: Expression levels of key gastric cancer stem cell (GCSC) markers, CypA, and CD147 in MKN45 adherent and tumorsphere cells. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for adherent cells was normalized to onefold. * p < 0.05 vs. the adherent cells.

    Techniques Used: Expressing, Western Blot

    Effects of C9 and CsA on the CypA/CD147-mediated signaling pathways in MKN45 GCSCs. ( A , B ) MKN45-derived GCSCs were treated with the indicated concentrations of each compound for 72 h. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for untreated control was normalized to onefold. * p < 0.05 vs. the control.
    Figure Legend Snippet: Effects of C9 and CsA on the CypA/CD147-mediated signaling pathways in MKN45 GCSCs. ( A , B ) MKN45-derived GCSCs were treated with the indicated concentrations of each compound for 72 h. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for untreated control was normalized to onefold. * p < 0.05 vs. the control.

    Techniques Used: Derivative Assay, Western Blot

    cd147 antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc cd147 antibody
    Expressions of CyPA and <t>CD147</t> are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Cd147 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness"

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    Journal: BioMed Research International

    doi: 10.1155/2020/7035847

    Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Figure Legend Snippet: Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Techniques Used: Expressing

    Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
    Figure Legend Snippet: Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.
    Figure Legend Snippet: Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Techniques Used: EdU Assay, Inhibition, Flow Cytometry

    Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.
    Figure Legend Snippet: Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Techniques Used: Expressing

    cd147  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc cd147
    Expressions of CyPA and <t>CD147</t> are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd147/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd147 - by Bioz Stars, 2024-06
    94/100 stars

    Images

    1) Product Images from "Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness"

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    Journal: BioMed Research International

    doi: 10.1155/2020/7035847

    Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Figure Legend Snippet: Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Techniques Used: Expressing

    Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
    Figure Legend Snippet: Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.
    Figure Legend Snippet: Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Techniques Used: EdU Assay, Inhibition, Flow Cytometry

    Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.
    Figure Legend Snippet: Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Techniques Used: Expressing

    cd147  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc cd147
    Epigenetic inactivation of CSC surface markers ( CD24 , CD44 , CD133 , and <t>CD147</t> ) in OSCC cell lines. Quantitative real-time RT-PCR analysis was carried out to assess the transcriptional expression level of ( A ) CD24 , ( B ) CD44 , ( C ) CD133 , and ( D ) CD147 genes in OSCC cell lines before and after treatment with 5-aza-dC (5 μM) for 72 h and TSA (0.3 μM) for 18 h. The expression levels of the genes were internally normalized to the expression levels of GAPDH , and the normalized expression for each gene before 5-aza-dC treatments was set to one. The asterisks indicate significant increases in gene expression after 5-aza-dC treatment ( p < 0.05). All data were statistically analyzed using the Student’s t -test. * p < 0.05; ** p < 0.01; *** p < 0.001; no asterisk, not significant. A two-tailed Student’s t -test was used to compare data between the two groups.
    Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Promoter Methylation of Cancer Stem Cell Surface Markers as an Epigenetic Biomarker for Prognosis of Oral Squamous Cell Carcinoma"

    Article Title: Promoter Methylation of Cancer Stem Cell Surface Markers as an Epigenetic Biomarker for Prognosis of Oral Squamous Cell Carcinoma

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232314624

    Epigenetic inactivation of CSC surface markers ( CD24 , CD44 , CD133 , and CD147 ) in OSCC cell lines. Quantitative real-time RT-PCR analysis was carried out to assess the transcriptional expression level of ( A ) CD24 , ( B ) CD44 , ( C ) CD133 , and ( D ) CD147 genes in OSCC cell lines before and after treatment with 5-aza-dC (5 μM) for 72 h and TSA (0.3 μM) for 18 h. The expression levels of the genes were internally normalized to the expression levels of GAPDH , and the normalized expression for each gene before 5-aza-dC treatments was set to one. The asterisks indicate significant increases in gene expression after 5-aza-dC treatment ( p < 0.05). All data were statistically analyzed using the Student’s t -test. * p < 0.05; ** p < 0.01; *** p < 0.001; no asterisk, not significant. A two-tailed Student’s t -test was used to compare data between the two groups.
    Figure Legend Snippet: Epigenetic inactivation of CSC surface markers ( CD24 , CD44 , CD133 , and CD147 ) in OSCC cell lines. Quantitative real-time RT-PCR analysis was carried out to assess the transcriptional expression level of ( A ) CD24 , ( B ) CD44 , ( C ) CD133 , and ( D ) CD147 genes in OSCC cell lines before and after treatment with 5-aza-dC (5 μM) for 72 h and TSA (0.3 μM) for 18 h. The expression levels of the genes were internally normalized to the expression levels of GAPDH , and the normalized expression for each gene before 5-aza-dC treatments was set to one. The asterisks indicate significant increases in gene expression after 5-aza-dC treatment ( p < 0.05). All data were statistically analyzed using the Student’s t -test. * p < 0.05; ** p < 0.01; *** p < 0.001; no asterisk, not significant. A two-tailed Student’s t -test was used to compare data between the two groups.

    Techniques Used: Quantitative RT-PCR, Expressing, Two Tailed Test

    Promoter DNA methylation analysis of the CD24 , CD44 , CD133 , and CD147 genes in OSCC cell lines, primary OSCC tumor tissues, and normal oral mucosal tissues. ( A ) Schematic structure of the CpG islands at the promoter regions for CD24 , CD44 , CD133 , and CD147. ( A ) The primer locations for MSP were indicated as an orange bar for each gene. ( B ) MSP results in OSCC cell lines. Gel pictures describe the methylation analysis by MSP. DNA methyltransferase 1 and 3b knockout HCT116 cells (DKO) were included as positive controls. The PCR products recognized the unmethylated (U) and methylated (M) genes. DKO cells were used for the unmethylated control. IVD = in vitro methylated control; ddH 2 O = water control containing no DNA. ( C ) Methylation frequency of the CD24 , CD133 , and CD147 genes between the samples from patients with OSCC ( n = 102) and the normal oral mucosal tissue samples ( n = 45).
    Figure Legend Snippet: Promoter DNA methylation analysis of the CD24 , CD44 , CD133 , and CD147 genes in OSCC cell lines, primary OSCC tumor tissues, and normal oral mucosal tissues. ( A ) Schematic structure of the CpG islands at the promoter regions for CD24 , CD44 , CD133 , and CD147. ( A ) The primer locations for MSP were indicated as an orange bar for each gene. ( B ) MSP results in OSCC cell lines. Gel pictures describe the methylation analysis by MSP. DNA methyltransferase 1 and 3b knockout HCT116 cells (DKO) were included as positive controls. The PCR products recognized the unmethylated (U) and methylated (M) genes. DKO cells were used for the unmethylated control. IVD = in vitro methylated control; ddH 2 O = water control containing no DNA. ( C ) Methylation frequency of the CD24 , CD133 , and CD147 genes between the samples from patients with OSCC ( n = 102) and the normal oral mucosal tissue samples ( n = 45).

    Techniques Used: DNA Methylation Assay, Methylation, Knock-Out, In Vitro

    Representative bisulfite sequencing results of the CpG islands in the CD24 , CD133 , and CD147 gene promoter regions in OSCC cell lines, primary OSCC tumors ( n = 3), and normal oral mucosal tissues ( n = 3). The locations of the CpG sites ( CD24 : upstream region from −903 to −770; CD133: exon 1 region from −230 to +90 ; and CD147: upstream region from −552 to −291) relative to the transcription start sites (TSSs) of exon 1 are shown. Each box represents a CpG dinucleotide. The black boxes represent methylated cytosines, and the white boxes represent unmethylated cytosines.
    Figure Legend Snippet: Representative bisulfite sequencing results of the CpG islands in the CD24 , CD133 , and CD147 gene promoter regions in OSCC cell lines, primary OSCC tumors ( n = 3), and normal oral mucosal tissues ( n = 3). The locations of the CpG sites ( CD24 : upstream region from −903 to −770; CD133: exon 1 region from −230 to +90 ; and CD147: upstream region from −552 to −291) relative to the transcription start sites (TSSs) of exon 1 are shown. Each box represents a CpG dinucleotide. The black boxes represent methylated cytosines, and the white boxes represent unmethylated cytosines.

    Techniques Used: Methylation Sequencing, Methylation

    Correlation analysis between gene expression level and promoter methylation level from the TCGA database. ( A , D ) The expression levels of CD133 (PROM1) and CD147 (BSG) in tumor tissues were inversely correlated with the promoter DNA methylation rates. Expression levels of ( A ) CD133 and ( D ) CD147 in primary OSCC tumors ( n = 328) compared to normal oral mucosa ( n = 32), extracted from the TCGA HNSCC database. ( B , E ) The methylation levels of the CpG sites of ( B ) CD133 and ( E ) CD147 in primary OSCC tumors ( n = 338) compared to normal oral mucosa ( n = 34), extracted from the HNSCC TCGA database. ( C , F ) Negative correlation between the gene expression and promoter methylation of ( C , F ). Spearman’s correlation analysis was performed between the methylation (vertical axis) and gene expression (horizontal axis) of the CD133 ( R = −0.20, p = 0.002686) and CD147 genes ( R = −0.17, p = 0.0002601). The Spearman’s correlation coefficients and p -values are shown in each plot.
    Figure Legend Snippet: Correlation analysis between gene expression level and promoter methylation level from the TCGA database. ( A , D ) The expression levels of CD133 (PROM1) and CD147 (BSG) in tumor tissues were inversely correlated with the promoter DNA methylation rates. Expression levels of ( A ) CD133 and ( D ) CD147 in primary OSCC tumors ( n = 328) compared to normal oral mucosa ( n = 32), extracted from the TCGA HNSCC database. ( B , E ) The methylation levels of the CpG sites of ( B ) CD133 and ( E ) CD147 in primary OSCC tumors ( n = 338) compared to normal oral mucosa ( n = 34), extracted from the HNSCC TCGA database. ( C , F ) Negative correlation between the gene expression and promoter methylation of ( C , F ). Spearman’s correlation analysis was performed between the methylation (vertical axis) and gene expression (horizontal axis) of the CD133 ( R = −0.20, p = 0.002686) and CD147 genes ( R = −0.17, p = 0.0002601). The Spearman’s correlation coefficients and p -values are shown in each plot.

    Techniques Used: Expressing, Methylation, DNA Methylation Assay

    Protein expression levels of CD133 and CD147 in primary OSCC tumors and normal oral mucosal tissue samples. The representative immunohistochemical analysis results show ( A ) CD133 and ( B ) CD147 expression in primary OSCC tissues and normal oral mucosal tissues (left images: × 40, scale bar, 50 µm; right images: × 20, scale bar, 100 µm).
    Figure Legend Snippet: Protein expression levels of CD133 and CD147 in primary OSCC tumors and normal oral mucosal tissue samples. The representative immunohistochemical analysis results show ( A ) CD133 and ( B ) CD147 expression in primary OSCC tissues and normal oral mucosal tissues (left images: × 40, scale bar, 50 µm; right images: × 20, scale bar, 100 µm).

    Techniques Used: Expressing, Immunohistochemical staining

    Kaplan-Meier curves showing the effect of CD133 and CD147 DNA methylation on overall survival among patients with OSCC ( n = 338) from the HNSCC TCGA dataset. The overall survival for ( A ) CD133 ( p = 0.018) and ( B ) CD147 ( p = 0.0054), respectively, is shown. A probability of <0.05 was considered to represent a statistically significant difference. ( C ) Combination of CD133 and CD147 gene methylation ( p = 0.025). The clinical information of patients with OSCC ( n = 338) was divided into two groups according to their methylation status (β-values) on CD133 (methylation high (red line), n = 209 and methylation low (blue line), n = 129), CD147 (methylation high (red line), n = 113 and methylation low (blue line), n = 55), and a combination of CD133 and CD147 (methylation high (red line), n = 143 and methylation low (blue line), n = 195).
    Figure Legend Snippet: Kaplan-Meier curves showing the effect of CD133 and CD147 DNA methylation on overall survival among patients with OSCC ( n = 338) from the HNSCC TCGA dataset. The overall survival for ( A ) CD133 ( p = 0.018) and ( B ) CD147 ( p = 0.0054), respectively, is shown. A probability of <0.05 was considered to represent a statistically significant difference. ( C ) Combination of CD133 and CD147 gene methylation ( p = 0.025). The clinical information of patients with OSCC ( n = 338) was divided into two groups according to their methylation status (β-values) on CD133 (methylation high (red line), n = 209 and methylation low (blue line), n = 129), CD147 (methylation high (red line), n = 113 and methylation low (blue line), n = 55), and a combination of CD133 and CD147 (methylation high (red line), n = 143 and methylation low (blue line), n = 195).

    Techniques Used: DNA Methylation Assay, Methylation

    antibodies against emmprin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies against emmprin
    Decrease in invasion activities of SaOS-2 cells transfected by <t>EMMPRIN</t> siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, <t>the</t> <t>membranes</t> were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm
    Antibodies Against Emmprin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "EMMPRIN expression is associated with metastatic progression in osteosarcoma"

    Article Title: EMMPRIN expression is associated with metastatic progression in osteosarcoma

    Journal: BMC Cancer

    doi: 10.1186/s12885-021-08774-9

    Decrease in invasion activities of SaOS-2 cells transfected by EMMPRIN siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, the membranes were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm
    Figure Legend Snippet: Decrease in invasion activities of SaOS-2 cells transfected by EMMPRIN siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, the membranes were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm

    Techniques Used: Transfection, Incubation, Light Microscopy, Matrigel Assay, Invasion Assay

    EMMPRIN silencing inhibits the metastatic nodes in the lungs. a Metastasis assay by tail vein injection of shRNA transfected 143B cells in BALB/c mice. Lungs were excised for examination at 8 weeks post-injection. The number of lung metastatic nodes was assessed by Bouin’s stain. Arrows indicate visible lung surface metastases. b Tumor volume and c Number of nodules at the study end point. d 143B cells were transfected with mock shRNA and EMMPRIN shRNA for 48 h, and cell lysates were analyzed by western blot using anti-EMMPRIN antibody. β-actin was used as a loading control. e Microscope images by H&E staining show that EMMPRIN silencing significantly inhibits the lung metastatic nodes after inoculation with 143B, 2 × 10 5 cells/ 200 ul. *, p < 0.05 compared with cells transfected with control siRNA. Scale bar, 100 μm. Original magnification, × 10 and × 40
    Figure Legend Snippet: EMMPRIN silencing inhibits the metastatic nodes in the lungs. a Metastasis assay by tail vein injection of shRNA transfected 143B cells in BALB/c mice. Lungs were excised for examination at 8 weeks post-injection. The number of lung metastatic nodes was assessed by Bouin’s stain. Arrows indicate visible lung surface metastases. b Tumor volume and c Number of nodules at the study end point. d 143B cells were transfected with mock shRNA and EMMPRIN shRNA for 48 h, and cell lysates were analyzed by western blot using anti-EMMPRIN antibody. β-actin was used as a loading control. e Microscope images by H&E staining show that EMMPRIN silencing significantly inhibits the lung metastatic nodes after inoculation with 143B, 2 × 10 5 cells/ 200 ul. *, p < 0.05 compared with cells transfected with control siRNA. Scale bar, 100 μm. Original magnification, × 10 and × 40

    Techniques Used: Injection, shRNA, Transfection, Staining, Western Blot, Microscopy

    rabbit monoclonal antibodies against cd147  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal antibodies against cd147
    mRNA and protein expression levels of <t>CD147</t> in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Rabbit Monoclonal Antibodies Against Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway"

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2020.9058

    mRNA and protein expression levels of CD147 in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Figure Legend Snippet: mRNA and protein expression levels of CD147 in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation, shRNA

    CD147 promotes LNCaP cell viability. LNCaP/shCD147 and LNCaP/Scramble cells were seeded in 96-well plates and incubated for 2, 4, 6 or 8 days. Cell viability was determined using a Cell Counting Kit-8 assay. Values are normalized to LNCaP/Scramble and presented as the mean ± standard deviation of three experiments. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Figure Legend Snippet: CD147 promotes LNCaP cell viability. LNCaP/shCD147 and LNCaP/Scramble cells were seeded in 96-well plates and incubated for 2, 4, 6 or 8 days. Cell viability was determined using a Cell Counting Kit-8 assay. Values are normalized to LNCaP/Scramble and presented as the mean ± standard deviation of three experiments. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Techniques Used: Incubation, Cell Counting, Standard Deviation, shRNA

    CD147 promotes the migration and invasion of LNCaP cells. Effect of CD147 knockdown on (A) migration and (B) invasion of LNCaP cells. Scale bars, 20 µm. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Figure Legend Snippet: CD147 promotes the migration and invasion of LNCaP cells. Effect of CD147 knockdown on (A) migration and (B) invasion of LNCaP cells. Scale bars, 20 µm. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Techniques Used: Migration, Standard Deviation, shRNA

    CD147 promotes epithelial-mesenchymal transition of LNCaP cells. The expression of the epithelial marker E-cadherin and the mesenchymal markers N-cadherin and vimentin was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Figure Legend Snippet: CD147 promotes epithelial-mesenchymal transition of LNCaP cells. The expression of the epithelial marker E-cadherin and the mesenchymal markers N-cadherin and vimentin was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Techniques Used: Expressing, Marker, Western Blot, Standard Deviation, shRNA

    CD147 regulates epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (A and B) Expression of β-catenin, Snail and p-GSK-3β (Ser9) was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. (C and D) Expression of β-catenin, Snail, p-β-catenin (Ser33/37/Thr41), vimentin and E-cadherin following treatment with or without LiCl in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values are presented as the mean ± standard deviation of three experiments. Lanes 1 and 3, LNCaP/Scramble group; Lanes 2 and 4, LNCaP/shCD147 group. * P<0.05, ** P<0.01 vs. LNCaP/Scramble; # P<0.05, ## P<0.01 vs. LNCaP/shCD147. LNCaP, lymph node carcinoma of the prostate; LiCl, lithium chloride; sh, short hairpin RNA; p-, phosphorylated; GSK-3β, glycogen synthase kinase-3β.
    Figure Legend Snippet: CD147 regulates epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (A and B) Expression of β-catenin, Snail and p-GSK-3β (Ser9) was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. (C and D) Expression of β-catenin, Snail, p-β-catenin (Ser33/37/Thr41), vimentin and E-cadherin following treatment with or without LiCl in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values are presented as the mean ± standard deviation of three experiments. Lanes 1 and 3, LNCaP/Scramble group; Lanes 2 and 4, LNCaP/shCD147 group. * P<0.05, ** P<0.01 vs. LNCaP/Scramble; # P<0.05, ## P<0.01 vs. LNCaP/shCD147. LNCaP, lymph node carcinoma of the prostate; LiCl, lithium chloride; sh, short hairpin RNA; p-, phosphorylated; GSK-3β, glycogen synthase kinase-3β.

    Techniques Used: Expressing, Western Blot, Standard Deviation, shRNA

    primary antibodies against cd147  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc primary antibodies against cd147
    <t>CD147-shRNA3</t> resulted in maximum inhibition of CD147 mRNA (A) and protein (B) expression levels. *, P<0.05 compared to the control group; @ , P<0.05 compared to CD147-shRNA1; $ , P<0.05 compared to CD147-shRNA2.
    Primary Antibodies Against Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against cd147/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    primary antibodies against cd147 - by Bioz Stars, 2024-06
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    Images

    1) Product Images from "CD147 promotes glucose metabolism, invasion and metastasis via PI3K/AKT pathway in oral squamous cell carcinomas"

    Article Title: CD147 promotes glucose metabolism, invasion and metastasis via PI3K/AKT pathway in oral squamous cell carcinomas

    Journal: Translational Cancer Research

    doi: 10.21037/tcr.2019.07.50

    CD147-shRNA3 resulted in maximum inhibition of CD147 mRNA (A) and protein (B) expression levels. *, P<0.05 compared to the control group; @ , P<0.05 compared to CD147-shRNA1; $ , P<0.05 compared to CD147-shRNA2.
    Figure Legend Snippet: CD147-shRNA3 resulted in maximum inhibition of CD147 mRNA (A) and protein (B) expression levels. *, P<0.05 compared to the control group; @ , P<0.05 compared to CD147-shRNA1; $ , P<0.05 compared to CD147-shRNA2.

    Techniques Used: Inhibition, Expressing

    CD147 silencing increased glucose uptake (left panel) and lactate secretion (right panel) in SCC-25 (A) and CAL-27 (B) cells in an shRNA dose-dependent manner. *, P<0.05 compared to the control group; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA.
    Figure Legend Snippet: CD147 silencing increased glucose uptake (left panel) and lactate secretion (right panel) in SCC-25 (A) and CAL-27 (B) cells in an shRNA dose-dependent manner. *, P<0.05 compared to the control group; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA.

    Techniques Used: shRNA

    CD147 regulated the expression of key proteins of the PI3K/AKT pathway in SCC-25 and CAL-27 cells. Western blot analyses of PI3K, p-PI3K, PDK1, p-PDK1, AKT and p-AKT levels in SCC-25 (A) and CAL-27 cells (B). *, P<0.05 compared to control; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA.
    Figure Legend Snippet: CD147 regulated the expression of key proteins of the PI3K/AKT pathway in SCC-25 and CAL-27 cells. Western blot analyses of PI3K, p-PI3K, PDK1, p-PDK1, AKT and p-AKT levels in SCC-25 (A) and CAL-27 cells (B). *, P<0.05 compared to control; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA.

    Techniques Used: Expressing, Western Blot, shRNA

    CD147 regulated glucose metabolism via the PI3K/AKT pathway in SCC-25 and CAL-27 cells. SCC-25 and CAL-27 cells were transfected with PI3K-overexpressing plasmid and CD147 shRNAs. The glucose content and lactate production (A,C), and GLUT-1 expression (B,D) in the co-transfected SCC-25 (A,B) and CAL-27 (C,D) cells. *, P<0.05 compared to control; @ , P<0.05 compared to PI3K OE group; $ , P<0.05 compared to CD147 shRNA vector group.
    Figure Legend Snippet: CD147 regulated glucose metabolism via the PI3K/AKT pathway in SCC-25 and CAL-27 cells. SCC-25 and CAL-27 cells were transfected with PI3K-overexpressing plasmid and CD147 shRNAs. The glucose content and lactate production (A,C), and GLUT-1 expression (B,D) in the co-transfected SCC-25 (A,B) and CAL-27 (C,D) cells. *, P<0.05 compared to control; @ , P<0.05 compared to PI3K OE group; $ , P<0.05 compared to CD147 shRNA vector group.

    Techniques Used: Transfection, Plasmid Preparation, Expressing, shRNA

    The effect of CD147 silencing on the invasion and metastasis of SCC-25 cells. *, P<0.05 compared to control; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA. Magnification: ×200, scale bar =50 µm; Crystal Violet Staining (0.1%).
    Figure Legend Snippet: The effect of CD147 silencing on the invasion and metastasis of SCC-25 cells. *, P<0.05 compared to control; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA. Magnification: ×200, scale bar =50 µm; Crystal Violet Staining (0.1%).

    Techniques Used: shRNA, Staining

    CD147 facilitated invasion and metastasis of SCC-25 (A) and CAL-27 (B) cells via the PI3K/AKT pathway. *, P<0.05 compared to control; @ , P<0.05 compared to PI3K OE group; $ , P<0.05 compared to the CD147 shRNA vector group. Magnification: ×100, scale bar =100 µm; Crystal Violet Staining (0.1%).
    Figure Legend Snippet: CD147 facilitated invasion and metastasis of SCC-25 (A) and CAL-27 (B) cells via the PI3K/AKT pathway. *, P<0.05 compared to control; @ , P<0.05 compared to PI3K OE group; $ , P<0.05 compared to the CD147 shRNA vector group. Magnification: ×100, scale bar =100 µm; Crystal Violet Staining (0.1%).

    Techniques Used: shRNA, Plasmid Preparation, Staining

    rabbit anti human basigin e1s1v  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti human basigin e1s1v
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    Rabbit Anti Human Basigin E1s1v, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism"

    Article Title: Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism

    Journal: Cell reports

    doi: 10.1016/j.celrep.2018.10.100

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Protease Inhibitor, Mutagenesis, Lysis, Transfection, Enzyme-linked Immunosorbent Assay, Lactate Assay, Immunoprecipitation, Bicinchoninic Acid Protein Assay, RNA Sequencing Assay, Binding Assay, Software

    13287s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 13287s
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    13287s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/13287s/product/Cell Signaling Technology Inc
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    13287s - by Bioz Stars, 2024-06
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    1) Product Images from "Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism"

    Article Title: Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism

    Journal: Cell reports

    doi: 10.1016/j.celrep.2018.10.100

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Protease Inhibitor, Mutagenesis, Lysis, Transfection, Enzyme-linked Immunosorbent Assay, Lactate Assay, Immunoprecipitation, Bicinchoninic Acid Protein Assay, RNA Sequencing Assay, Binding Assay, Software

    rabbit anti human cd147  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti human cd147
    Rabbit Anti Human Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human cd147/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc cd147
    Expression levels of key gastric cancer stem cell (GCSC) markers, CypA, and <t>CD147</t> in MKN45 adherent and tumorsphere cells. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for adherent cells was normalized to onefold. * p < 0.05 vs. the adherent cells.
    Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc cd147 antibody
    Expressions of CyPA and <t>CD147</t> are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
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    Decrease in invasion activities of SaOS-2 cells transfected by <t>EMMPRIN</t> siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, <t>the</t> <t>membranes</t> were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm
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    Cell Signaling Technology Inc rabbit monoclonal antibodies against cd147
    mRNA and protein expression levels of <t>CD147</t> in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Rabbit Monoclonal Antibodies Against Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc primary antibodies against cd147
    <t>CD147-shRNA3</t> resulted in maximum inhibition of CD147 mRNA (A) and protein (B) expression levels. *, P<0.05 compared to the control group; @ , P<0.05 compared to CD147-shRNA1; $ , P<0.05 compared to CD147-shRNA2.
    Primary Antibodies Against Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit anti human basigin e1s1v
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    Image Search Results


    Expression levels of key gastric cancer stem cell (GCSC) markers, CypA, and CD147 in MKN45 adherent and tumorsphere cells. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for adherent cells was normalized to onefold. * p < 0.05 vs. the adherent cells.

    Journal: International Journal of Molecular Sciences

    Article Title: Cyclophilin A Inhibitors Suppress Proliferation and Induce Apoptosis of MKN45 Gastric Cancer Stem-like Cells by Regulating CypA/CD147-Mediated Signaling Pathway

    doi: 10.3390/ijms24054734

    Figure Lengend Snippet: Expression levels of key gastric cancer stem cell (GCSC) markers, CypA, and CD147 in MKN45 adherent and tumorsphere cells. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for adherent cells was normalized to onefold. * p < 0.05 vs. the adherent cells.

    Article Snippet: Antibodies against CypA (#2175), CD147 (#13287), CD44 (#37259), CD133 (#64326), integrin α6 (#3750), Sox2 (#3579), Nanog (#3580), Oct4 (#2750), ALDH1A1 (#12035), p53 (#2524), p21 Waf1/Cip1 (#2947), cleaved caspase-9 (#9501), cleaved caspase-3 (#9661), cleaved PARP (#9542), phospho-AKT (#4060), AKT (#9272), phospho-ERK1/2 (#9101), ERK1/2 (#9102), phospho-p38 (#4511), p38 (#8690), rabbit IgG (#7074), and mouse IgG (#7076) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Expressing, Western Blot

    Effects of C9 and CsA on the CypA/CD147-mediated signaling pathways in MKN45 GCSCs. ( A , B ) MKN45-derived GCSCs were treated with the indicated concentrations of each compound for 72 h. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for untreated control was normalized to onefold. * p < 0.05 vs. the control.

    Journal: International Journal of Molecular Sciences

    Article Title: Cyclophilin A Inhibitors Suppress Proliferation and Induce Apoptosis of MKN45 Gastric Cancer Stem-like Cells by Regulating CypA/CD147-Mediated Signaling Pathway

    doi: 10.3390/ijms24054734

    Figure Lengend Snippet: Effects of C9 and CsA on the CypA/CD147-mediated signaling pathways in MKN45 GCSCs. ( A , B ) MKN45-derived GCSCs were treated with the indicated concentrations of each compound for 72 h. Protein levels were detected via Western blot analysis using specific antibodies and were further quantified using densitometry. β-actin levels were used as an internal control. The ratio of each target protein to β-actin for untreated control was normalized to onefold. * p < 0.05 vs. the control.

    Article Snippet: Antibodies against CypA (#2175), CD147 (#13287), CD44 (#37259), CD133 (#64326), integrin α6 (#3750), Sox2 (#3579), Nanog (#3580), Oct4 (#2750), ALDH1A1 (#12035), p53 (#2524), p21 Waf1/Cip1 (#2947), cleaved caspase-9 (#9501), cleaved caspase-3 (#9661), cleaved PARP (#9542), phospho-AKT (#4060), AKT (#9272), phospho-ERK1/2 (#9101), ERK1/2 (#9102), phospho-p38 (#4511), p38 (#8690), rabbit IgG (#7074), and mouse IgG (#7076) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Derivative Assay, Western Blot

    Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Article Snippet: After the blockade of nonspecific binding, tissue sections were incubated with the Cyclophilin A antibody (cat. no. 5360s; 1 : 150 dilution; Santa Cruz Biotechnology, Inc.) and CD147 antibody (cat. no. 13287; 1 : 400 dilution; Cell Signaling Technology, Inc.) for overnight at 4 centigrade.

    Techniques: Expressing

    Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Article Snippet: After the blockade of nonspecific binding, tissue sections were incubated with the Cyclophilin A antibody (cat. no. 5360s; 1 : 150 dilution; Santa Cruz Biotechnology, Inc.) and CD147 antibody (cat. no. 13287; 1 : 400 dilution; Cell Signaling Technology, Inc.) for overnight at 4 centigrade.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Article Snippet: After the blockade of nonspecific binding, tissue sections were incubated with the Cyclophilin A antibody (cat. no. 5360s; 1 : 150 dilution; Santa Cruz Biotechnology, Inc.) and CD147 antibody (cat. no. 13287; 1 : 400 dilution; Cell Signaling Technology, Inc.) for overnight at 4 centigrade.

    Techniques: EdU Assay, Inhibition, Flow Cytometry

    Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Article Snippet: After the blockade of nonspecific binding, tissue sections were incubated with the Cyclophilin A antibody (cat. no. 5360s; 1 : 150 dilution; Santa Cruz Biotechnology, Inc.) and CD147 antibody (cat. no. 13287; 1 : 400 dilution; Cell Signaling Technology, Inc.) for overnight at 4 centigrade.

    Techniques: Expressing

    Decrease in invasion activities of SaOS-2 cells transfected by EMMPRIN siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, the membranes were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm

    Journal: BMC Cancer

    Article Title: EMMPRIN expression is associated with metastatic progression in osteosarcoma

    doi: 10.1186/s12885-021-08774-9

    Figure Lengend Snippet: Decrease in invasion activities of SaOS-2 cells transfected by EMMPRIN siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, the membranes were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm

    Article Snippet: The membranes were probed with primary antibodies against EMMPRIN (Cell Signaling, #13287S, Lot1) after incubating in a blocking buffer.

    Techniques: Transfection, Incubation, Light Microscopy, Matrigel Assay, Invasion Assay

    EMMPRIN silencing inhibits the metastatic nodes in the lungs. a Metastasis assay by tail vein injection of shRNA transfected 143B cells in BALB/c mice. Lungs were excised for examination at 8 weeks post-injection. The number of lung metastatic nodes was assessed by Bouin’s stain. Arrows indicate visible lung surface metastases. b Tumor volume and c Number of nodules at the study end point. d 143B cells were transfected with mock shRNA and EMMPRIN shRNA for 48 h, and cell lysates were analyzed by western blot using anti-EMMPRIN antibody. β-actin was used as a loading control. e Microscope images by H&E staining show that EMMPRIN silencing significantly inhibits the lung metastatic nodes after inoculation with 143B, 2 × 10 5 cells/ 200 ul. *, p < 0.05 compared with cells transfected with control siRNA. Scale bar, 100 μm. Original magnification, × 10 and × 40

    Journal: BMC Cancer

    Article Title: EMMPRIN expression is associated with metastatic progression in osteosarcoma

    doi: 10.1186/s12885-021-08774-9

    Figure Lengend Snippet: EMMPRIN silencing inhibits the metastatic nodes in the lungs. a Metastasis assay by tail vein injection of shRNA transfected 143B cells in BALB/c mice. Lungs were excised for examination at 8 weeks post-injection. The number of lung metastatic nodes was assessed by Bouin’s stain. Arrows indicate visible lung surface metastases. b Tumor volume and c Number of nodules at the study end point. d 143B cells were transfected with mock shRNA and EMMPRIN shRNA for 48 h, and cell lysates were analyzed by western blot using anti-EMMPRIN antibody. β-actin was used as a loading control. e Microscope images by H&E staining show that EMMPRIN silencing significantly inhibits the lung metastatic nodes after inoculation with 143B, 2 × 10 5 cells/ 200 ul. *, p < 0.05 compared with cells transfected with control siRNA. Scale bar, 100 μm. Original magnification, × 10 and × 40

    Article Snippet: The membranes were probed with primary antibodies against EMMPRIN (Cell Signaling, #13287S, Lot1) after incubating in a blocking buffer.

    Techniques: Injection, shRNA, Transfection, Staining, Western Blot, Microscopy

    mRNA and protein expression levels of CD147 in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: mRNA and protein expression levels of CD147 in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation, shRNA

    CD147 promotes LNCaP cell viability. LNCaP/shCD147 and LNCaP/Scramble cells were seeded in 96-well plates and incubated for 2, 4, 6 or 8 days. Cell viability was determined using a Cell Counting Kit-8 assay. Values are normalized to LNCaP/Scramble and presented as the mean ± standard deviation of three experiments. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: CD147 promotes LNCaP cell viability. LNCaP/shCD147 and LNCaP/Scramble cells were seeded in 96-well plates and incubated for 2, 4, 6 or 8 days. Cell viability was determined using a Cell Counting Kit-8 assay. Values are normalized to LNCaP/Scramble and presented as the mean ± standard deviation of three experiments. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Incubation, Cell Counting, Standard Deviation, shRNA

    CD147 promotes the migration and invasion of LNCaP cells. Effect of CD147 knockdown on (A) migration and (B) invasion of LNCaP cells. Scale bars, 20 µm. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: CD147 promotes the migration and invasion of LNCaP cells. Effect of CD147 knockdown on (A) migration and (B) invasion of LNCaP cells. Scale bars, 20 µm. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Migration, Standard Deviation, shRNA

    CD147 promotes epithelial-mesenchymal transition of LNCaP cells. The expression of the epithelial marker E-cadherin and the mesenchymal markers N-cadherin and vimentin was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: CD147 promotes epithelial-mesenchymal transition of LNCaP cells. The expression of the epithelial marker E-cadherin and the mesenchymal markers N-cadherin and vimentin was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Expressing, Marker, Western Blot, Standard Deviation, shRNA

    CD147 regulates epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (A and B) Expression of β-catenin, Snail and p-GSK-3β (Ser9) was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. (C and D) Expression of β-catenin, Snail, p-β-catenin (Ser33/37/Thr41), vimentin and E-cadherin following treatment with or without LiCl in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values are presented as the mean ± standard deviation of three experiments. Lanes 1 and 3, LNCaP/Scramble group; Lanes 2 and 4, LNCaP/shCD147 group. * P<0.05, ** P<0.01 vs. LNCaP/Scramble; # P<0.05, ## P<0.01 vs. LNCaP/shCD147. LNCaP, lymph node carcinoma of the prostate; LiCl, lithium chloride; sh, short hairpin RNA; p-, phosphorylated; GSK-3β, glycogen synthase kinase-3β.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: CD147 regulates epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (A and B) Expression of β-catenin, Snail and p-GSK-3β (Ser9) was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. (C and D) Expression of β-catenin, Snail, p-β-catenin (Ser33/37/Thr41), vimentin and E-cadherin following treatment with or without LiCl in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values are presented as the mean ± standard deviation of three experiments. Lanes 1 and 3, LNCaP/Scramble group; Lanes 2 and 4, LNCaP/shCD147 group. * P<0.05, ** P<0.01 vs. LNCaP/Scramble; # P<0.05, ## P<0.01 vs. LNCaP/shCD147. LNCaP, lymph node carcinoma of the prostate; LiCl, lithium chloride; sh, short hairpin RNA; p-, phosphorylated; GSK-3β, glycogen synthase kinase-3β.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Expressing, Western Blot, Standard Deviation, shRNA

    CD147-shRNA3 resulted in maximum inhibition of CD147 mRNA (A) and protein (B) expression levels. *, P<0.05 compared to the control group; @ , P<0.05 compared to CD147-shRNA1; $ , P<0.05 compared to CD147-shRNA2.

    Journal: Translational Cancer Research

    Article Title: CD147 promotes glucose metabolism, invasion and metastasis via PI3K/AKT pathway in oral squamous cell carcinomas

    doi: 10.21037/tcr.2019.07.50

    Figure Lengend Snippet: CD147-shRNA3 resulted in maximum inhibition of CD147 mRNA (A) and protein (B) expression levels. *, P<0.05 compared to the control group; @ , P<0.05 compared to CD147-shRNA1; $ , P<0.05 compared to CD147-shRNA2.

    Article Snippet: The proteins were electro-transferred to PVDF membranes, and blocked with 5% albumin bovine V (10735086001, Roche, USA) in Tris-buffered saline containing 0.5% Tween 20 (TBST buffer) at room temperature for 2 h. The membranes were then incubated overnight with the specific primary antibodies against CD147 (#13287), PI3K (#4249), p-PI3K (#4228), PDK1 (#5662), p-PDK1 (#3438), AKT (#9272), p-AKT (#4058), GLUT-1 (#12939) and GAPDH (#8884; all from Cell Signal Technology, USA).

    Techniques: Inhibition, Expressing

    CD147 silencing increased glucose uptake (left panel) and lactate secretion (right panel) in SCC-25 (A) and CAL-27 (B) cells in an shRNA dose-dependent manner. *, P<0.05 compared to the control group; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA.

    Journal: Translational Cancer Research

    Article Title: CD147 promotes glucose metabolism, invasion and metastasis via PI3K/AKT pathway in oral squamous cell carcinomas

    doi: 10.21037/tcr.2019.07.50

    Figure Lengend Snippet: CD147 silencing increased glucose uptake (left panel) and lactate secretion (right panel) in SCC-25 (A) and CAL-27 (B) cells in an shRNA dose-dependent manner. *, P<0.05 compared to the control group; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA.

    Article Snippet: The proteins were electro-transferred to PVDF membranes, and blocked with 5% albumin bovine V (10735086001, Roche, USA) in Tris-buffered saline containing 0.5% Tween 20 (TBST buffer) at room temperature for 2 h. The membranes were then incubated overnight with the specific primary antibodies against CD147 (#13287), PI3K (#4249), p-PI3K (#4228), PDK1 (#5662), p-PDK1 (#3438), AKT (#9272), p-AKT (#4058), GLUT-1 (#12939) and GAPDH (#8884; all from Cell Signal Technology, USA).

    Techniques: shRNA

    CD147 regulated the expression of key proteins of the PI3K/AKT pathway in SCC-25 and CAL-27 cells. Western blot analyses of PI3K, p-PI3K, PDK1, p-PDK1, AKT and p-AKT levels in SCC-25 (A) and CAL-27 cells (B). *, P<0.05 compared to control; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA.

    Journal: Translational Cancer Research

    Article Title: CD147 promotes glucose metabolism, invasion and metastasis via PI3K/AKT pathway in oral squamous cell carcinomas

    doi: 10.21037/tcr.2019.07.50

    Figure Lengend Snippet: CD147 regulated the expression of key proteins of the PI3K/AKT pathway in SCC-25 and CAL-27 cells. Western blot analyses of PI3K, p-PI3K, PDK1, p-PDK1, AKT and p-AKT levels in SCC-25 (A) and CAL-27 cells (B). *, P<0.05 compared to control; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA.

    Article Snippet: The proteins were electro-transferred to PVDF membranes, and blocked with 5% albumin bovine V (10735086001, Roche, USA) in Tris-buffered saline containing 0.5% Tween 20 (TBST buffer) at room temperature for 2 h. The membranes were then incubated overnight with the specific primary antibodies against CD147 (#13287), PI3K (#4249), p-PI3K (#4228), PDK1 (#5662), p-PDK1 (#3438), AKT (#9272), p-AKT (#4058), GLUT-1 (#12939) and GAPDH (#8884; all from Cell Signal Technology, USA).

    Techniques: Expressing, Western Blot, shRNA

    CD147 regulated glucose metabolism via the PI3K/AKT pathway in SCC-25 and CAL-27 cells. SCC-25 and CAL-27 cells were transfected with PI3K-overexpressing plasmid and CD147 shRNAs. The glucose content and lactate production (A,C), and GLUT-1 expression (B,D) in the co-transfected SCC-25 (A,B) and CAL-27 (C,D) cells. *, P<0.05 compared to control; @ , P<0.05 compared to PI3K OE group; $ , P<0.05 compared to CD147 shRNA vector group.

    Journal: Translational Cancer Research

    Article Title: CD147 promotes glucose metabolism, invasion and metastasis via PI3K/AKT pathway in oral squamous cell carcinomas

    doi: 10.21037/tcr.2019.07.50

    Figure Lengend Snippet: CD147 regulated glucose metabolism via the PI3K/AKT pathway in SCC-25 and CAL-27 cells. SCC-25 and CAL-27 cells were transfected with PI3K-overexpressing plasmid and CD147 shRNAs. The glucose content and lactate production (A,C), and GLUT-1 expression (B,D) in the co-transfected SCC-25 (A,B) and CAL-27 (C,D) cells. *, P<0.05 compared to control; @ , P<0.05 compared to PI3K OE group; $ , P<0.05 compared to CD147 shRNA vector group.

    Article Snippet: The proteins were electro-transferred to PVDF membranes, and blocked with 5% albumin bovine V (10735086001, Roche, USA) in Tris-buffered saline containing 0.5% Tween 20 (TBST buffer) at room temperature for 2 h. The membranes were then incubated overnight with the specific primary antibodies against CD147 (#13287), PI3K (#4249), p-PI3K (#4228), PDK1 (#5662), p-PDK1 (#3438), AKT (#9272), p-AKT (#4058), GLUT-1 (#12939) and GAPDH (#8884; all from Cell Signal Technology, USA).

    Techniques: Transfection, Plasmid Preparation, Expressing, shRNA

    The effect of CD147 silencing on the invasion and metastasis of SCC-25 cells. *, P<0.05 compared to control; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA. Magnification: ×200, scale bar =50 µm; Crystal Violet Staining (0.1%).

    Journal: Translational Cancer Research

    Article Title: CD147 promotes glucose metabolism, invasion and metastasis via PI3K/AKT pathway in oral squamous cell carcinomas

    doi: 10.21037/tcr.2019.07.50

    Figure Lengend Snippet: The effect of CD147 silencing on the invasion and metastasis of SCC-25 cells. *, P<0.05 compared to control; @ , P<0.05 compared to 50 ng/mL CD147 shRNA; $ , P<0.05 compared to 100 ng/mL CD147 shRNA; & , P<0.05 compared to 150 ng/mL CD147 shRNA. Magnification: ×200, scale bar =50 µm; Crystal Violet Staining (0.1%).

    Article Snippet: The proteins were electro-transferred to PVDF membranes, and blocked with 5% albumin bovine V (10735086001, Roche, USA) in Tris-buffered saline containing 0.5% Tween 20 (TBST buffer) at room temperature for 2 h. The membranes were then incubated overnight with the specific primary antibodies against CD147 (#13287), PI3K (#4249), p-PI3K (#4228), PDK1 (#5662), p-PDK1 (#3438), AKT (#9272), p-AKT (#4058), GLUT-1 (#12939) and GAPDH (#8884; all from Cell Signal Technology, USA).

    Techniques: shRNA, Staining

    CD147 facilitated invasion and metastasis of SCC-25 (A) and CAL-27 (B) cells via the PI3K/AKT pathway. *, P<0.05 compared to control; @ , P<0.05 compared to PI3K OE group; $ , P<0.05 compared to the CD147 shRNA vector group. Magnification: ×100, scale bar =100 µm; Crystal Violet Staining (0.1%).

    Journal: Translational Cancer Research

    Article Title: CD147 promotes glucose metabolism, invasion and metastasis via PI3K/AKT pathway in oral squamous cell carcinomas

    doi: 10.21037/tcr.2019.07.50

    Figure Lengend Snippet: CD147 facilitated invasion and metastasis of SCC-25 (A) and CAL-27 (B) cells via the PI3K/AKT pathway. *, P<0.05 compared to control; @ , P<0.05 compared to PI3K OE group; $ , P<0.05 compared to the CD147 shRNA vector group. Magnification: ×100, scale bar =100 µm; Crystal Violet Staining (0.1%).

    Article Snippet: The proteins were electro-transferred to PVDF membranes, and blocked with 5% albumin bovine V (10735086001, Roche, USA) in Tris-buffered saline containing 0.5% Tween 20 (TBST buffer) at room temperature for 2 h. The membranes were then incubated overnight with the specific primary antibodies against CD147 (#13287), PI3K (#4249), p-PI3K (#4228), PDK1 (#5662), p-PDK1 (#3438), AKT (#9272), p-AKT (#4058), GLUT-1 (#12939) and GAPDH (#8884; all from Cell Signal Technology, USA).

    Techniques: shRNA, Plasmid Preparation, Staining

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism

    doi: 10.1016/j.celrep.2018.10.100

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit anti-human Basigin (E1S1V) , Cell Signaling Technology , Cat. # 13287S.

    Techniques: Recombinant, Protease Inhibitor, Mutagenesis, Lysis, Transfection, Enzyme-linked Immunosorbent Assay, Lactate Assay, Immunoprecipitation, Bicinchoninic Acid Protein Assay, RNA Sequencing Assay, Binding Assay, Software

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism

    doi: 10.1016/j.celrep.2018.10.100

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit anti-human Basigin (E1S1V) , Cell Signaling Technology , Cat. # 13287S.

    Techniques: Recombinant, Protease Inhibitor, Mutagenesis, Lysis, Transfection, Enzyme-linked Immunosorbent Assay, Lactate Assay, Immunoprecipitation, Bicinchoninic Acid Protein Assay, RNA Sequencing Assay, Binding Assay, Software