Journal: Developmental cell

Article Title: Niche-specific factors dynamically regulate sebaceous gland stem cells in the skin

doi: 10.1016/j.devcel.2019.08.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: All mice were maintained in specific pathogen free housing and were used in accordance with regulations established by the University of Michigan Unit for Laboratory Animal Medicine. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-androgen receptor Santa Cruz Cat # sc-816 Rabbit anti-cleaved caspase-3 Cell Signaling Cat # 9661 Mouse anti-FASN BD Cat # 610963 Rabbit anti-Gata6 Cell Signaling Cat # D61E4 Chicken anti-GFP Aves Labs Cat # GFP-1010 Rabbit anti-Hes1 Cell Signaling Cat # 11988S Rabbit anti-Ki67 Cell Signaling Cat # 12202S Guinea Pig anti-K5 American Research Products Cat # 03-GP-CK5 Goat anti-K14 Santa Cruz (SC) Cat # sc-17104 Rabbit anti-K14 Covance (COV) Cat # PRB-155P Rabbit anti-K17 Coulombe Lab N/A Goat anti-K79 Santa Cruz Cat # sc-243156 Rabbit anti-K79 (NOTE: see Veniaminova et al., 2013 ) Abcam Cat # Ab7195 Goat anti-Lrig1 R&D Systems Cat # AF3688-SP Rabbit anti-NICD Cell Signaling Cat # 4147P Rabbit anti-Notch1 Cell Signaling Cat # 3608S Rabbit anti-PPARy Santa Cruz Cat # sc-7196 Rabbit anti-Rbpj Cell Signaling Cat # 5313P Goat anti-Tslp R&D Systems Cat # AF555-SP Biological Samples Mouse skin samples, obtained in accordance with guidelines established by the University of Michigan Unit for Laboratory Animal Medicine This manuscript Study protocol # PRO00008196 Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat # T5648 TSA Fluorescein Plus kit Perkin Elmer Cat # NEL741E001KT Nile Red Sigma Cat # N3013 RNeasy Mini Kit Qiagen Cat # 74104 Deposited Data RNA-seq data This manuscript GEO: {"type":"entrez-geo","attrs":{"text":"GSE125737","term_id":"125737"}} GSE125737 Experimental Models: Organisms/Strains Mouse: Lrig1 tm1.1(cre/ERT2)Rjc (Lrig1-Cre ERT2 ) Powell et al., 2012 ; The Jackson Laboratory Cat # 018418 Mouse: Tg(KRT14-cre/ERT)20Efu/J (K14-Cre ERT ) Vasioukhin et al., 1999 ; The Jackson Laboratory Cat # 005107 Mouse: Rbpj tm1Hon (Rbp-j flox ) Yamamoto et al., 2003 ; Laboratory of Dr. Tasuku Honjo MGI:3583755 Mouse: Notch1 tm2Rko /GridJ (Notch1 flox ) Yang et al., 2004 ; The Jackson Laboratory Cat # 007181 Mouse: Gt(ROSA)26Sor tm1(Smo/EYFP)Amc /J (SmoM2) Mao et al., 2006 ; The Jackson Laboratory Cat # 005130 Mouse: Krt79 tm2b(KOMP)Wtsi (K79-KO) Mesler et al., 2017 ; Laboratory of Dr.

Techniques: Recombinant, Software