tom20 (Proteintech)
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Proteintech
tom20
Tom20, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1346 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zebrafish+transcriptomic+data+set/TOM20+Antibody/pmc08096277-85-45-28
Average 96 stars, based on 1346 article reviews
Tom20, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1346 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zebrafish+transcriptomic+data+set/TOM20+Antibody/pmc08096277-85-45-28
Average 96 stars, based on 1346 article reviews
tom20 - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency"
Article Title: Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency
Journal: Nucleic Acids Research
doi: 10.1093/nar/gkab228
Figure Legend Snippet: Western blotting analysis of mitochondrial proteins. ( A , C ) Twenty micrograms of total proteins from mutant and WT zebrafish were electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with antibodies for 5 subunits of OXPHOS (mitochondrion-encoding Nd1, Co2 and Atp8 and nucleus-encoding Sdha and Atp5a), Tufm, Tfam and Tom20 as a loading control. Quantification of levels of OXPHOS subunits ( B ) and other mitochondrial proteins ( D ). Average contents of Nd1, Co2, Atp8, Atp5a, Sdha, Tufm and Tfam were normalized to the average content of Tom20 in mutant and WT zebrafish. The values for the mutant zebrafish are expressed as percentages of the average values for the WT zebrafish. The calculations were based on three independent determinations. Graph details and symbols are explained in the legend to Figure .
Techniques Used: Western Blot, Mutagenesis
Figure Legend Snippet: The synergic effects between Mto1 and Mtpap on the polyadenylation of mRNAs. ( A, B ) Immunoprecipitation analysis of MTO1 with MTPAP. HEK 293T cells transiently expressing with or without MTO1-FLAG were solubilized with a lysis buffer and lysate proteins were immuno-precipitated with immunocapture buffer (left) (input) and FLAG-antibody (right) (IP), respectively. Immunoprecipitates were analyzed by SDS-PAGE and Western blotting using anti-FLAG, anti-MTPAP and TOM20 antibodies, respectively. ( C ) Western blot analysis. Twenty micrograms of total proteins from mto1 −/− and WT zebrafish were electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with antibodies for Mtpap and Gapdh as a loading control. ( D ) Polyadenylation profiles of mitochondrial mRNAs upon mto1 −/− and WT zebrafish hearts. The 3′ termini of nd1, cytb , cox1 and cox3 mRNAs were assessed by RT-PCR amplifications from polyadenylated and oligoadenylated RNAs of mto1 −/− and WT zebrafish hearts and 3% agarose gel electrophoresis. Arrows indicated the positions of PCR products from the polyadenylated and oligoadenylated RNAs, respectively. ( E ) Quantification of poly(A) proportions of nd1, cytb , cox1 and cox3 transcripts in the WT and mto1 −/− zebrafish. ( F ) Poly(A) tail lengths from individually sequenced clones after 3’ end RACE analysis of nd1 transcripts in the mto1 −/− and WT zebrafish hearts. Graph details and symbols are explained in the legend to Figure .
Techniques Used: Immunoprecipitation, Expressing, Lysis, SDS Page, Western Blot, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Clone Assay
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