sequences for yap1 (OriGene)
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sequences for yap1
Sequences For Yap1, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap1+coding+sequence/pmc12722425-320-5-8?v=OriGene
Average 93 stars, based on 1 article reviews
Sequences For Yap1, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap1+coding+sequence/pmc12722425-320-5-8?v=OriGene
Average 93 stars, based on 1 article reviews
sequences for yap1 - by Bioz Stars,
2026-08
93/100 stars
Images
1) Product Images from "Exosomal miR-212-5p promotes tendon repair via targeting FOXO1 to activate PP1A/YAP1 signaling"
Article Title: Exosomal miR-212-5p promotes tendon repair via targeting FOXO1 to activate PP1A/YAP1 signaling
Journal: Communications Biology
doi: 10.1038/s42003-025-09210-5
Figure Legend Snippet: A qPCR was performed to assess FOXO1 expression in TDSCs after treatment with exosomes derived from miR-212-5p -depleted ADSCs. B Western blot was conducted to evaluate FOXO1 and p-YAP1 levels after treatment with exosomes derived from miR-212-5p-depleted ADSCs. C Bioinformatics analysis using the Starbase database was performed to predict the target interaction between miR-212-5p and FOXO1 . D qPCR was used to measure miR-212-5p levels in TDSCs after transfection with miR-212-5p mimics. E Dual-luciferase reporter assay was used to assess luciferase activity after treatment with exosomes derived from miR-212-5p -depleted or miR-212-5p -overexpressing ADSCs. F RNA pull-down assay was conducted using biotin-labeled miR-212-5p to evaluate its binding interaction with FOXO 1. G qPCR was performed to assess FOXO 1 expression after transfection with shFOXO1 in ADSCs. H Western blot analysis was used to evaluate FOXO1 and p-YAP1 levels after knockdown of miR-212-5p in ADSC-Exos and simultaneous knockdown of FOXO1. Data are presented as mean ± SD, and statistical analysis was conducted using a student t-test (for D , F – G ) of one-way ANOVA followed by Tukey’s post hoc test (for A , B , E , H ). n = 3.*p < 0.05, **p < 0.01, ***p < 0.001.
Techniques Used: Expressing, Derivative Assay, Western Blot, Transfection, Luciferase, Reporter Assay, Activity Assay, Pull Down Assay, Labeling, Binding Assay, Knockdown
Figure Legend Snippet: A , B qPCR and Western blot were performed to analyze PP1A levels in TDSCs transfected with shNC or shFOXO1 after treatment with exosomes derived from miR-212-5p-depleted ADSCs. C The JASPAR database was used to predict FOXO1 binding sites on the PP1A promoter. D ChIP assay was conducted using a FOXO1 antibody to assess its binding to the PP1A promoter. E , F qPCR and Western blot were performed to evaluate FOXO1 expression in TDSCs after transfection with FOXO1 overexpression vectors. G Dual-luciferase reporter assay was used to investigate the impact of FOXO1 knockdown and overexpression on PP1A transcriptional activity. H qPCR was performed to assess PP1A expression following FOXO1 knockdown and overexpression in TDSCs. I qPCR was conducted to evaluate PP1A expression after transfection with shPP1A in TDSCs. J Western blot was performed to study the effects of FOXO1 and PP1A knockdown on PP1A and p-YAP1 levels. K Immunofluorescence was used to examine nuclear YAP1 levels (green) in TDSCs after FOXO1 and PP1A knockdown. Scale bar: 100 μm. Data are presented as mean ± SD, and statistical analysis was conducted using a student t-test (for D – F , I ) of one-way ANOVA followed by Tukey’s post hoc test (for A , B , G , H , J ). n = 3 **p < 0.01, ***p < 0.001.
Techniques Used: Western Blot, Transfection, Derivative Assay, Binding Assay, Expressing, Over Expression, Luciferase, Reporter Assay, Knockdown, Activity Assay, Immunofluorescence
Figure Legend Snippet: A Western blot was performed to analyze the effects of transfection with wild-type YAP1 (YAP1-WT) expression vectors or S127A mutant YAP1 expression vectors (YAP1-S127A) on YAP1 and p-YAP1 levels. B – F MTT, EdU staining, scratch assay, Transwell assay, and Western blot were used to evaluate TDSC proliferation, migration, and the expression of tenogenic differentiation markers, including TNC, TNMD, Scx, COL1A1, and GAPDH, after FOXO1 overexpression with simultaneous overexpression of the S127A mutant YAP1. Scale bar: 100 or 500 μm. G Alcian Blue staining was performed to evaluate cartilage-like matrix deposition in TDSCs. Scale bar: 100 μm. Data are presented as mean ± SD, and statistical analysis was conducted using one-way ANOVA followed by Tukey’s post hoc test. n = 3. **p < 0.01, ***p < 0.001.
Techniques Used: Western Blot, Transfection, Expressing, Mutagenesis, Staining, Wound Healing Assay, Transwell Assay, Migration, Over Expression
Figure Legend Snippet: A Hematoxylin and eosin (HE) staining was performed to evaluate the arrangement of fibrous tissue in tendons from mice treated with exosomes derived from normal or miR-212-5p -depleted ADSCs. Scale bar: 50 or 20 μm. B Safranin O/Fast Green staining of tendon sections to evaluate ectopic cartilage-like matrix formation after injury in different groups of mice. Scale bar: 50 or 20 μm. C Immunohistochemistry (IHC) was used to assess the levels of TNMD, Scx, COL1A1, and SOX9 in tendon tissues from different groups of mice. Scale bar: 20 μm D , E Western blot analysis was conducted to examine the expression of tenogenic differentiation markers, including TNC, TNMD, Scx, and COL1A1, as well as PP1A, FOXO1, and p-YAP1 levels in tendon tissues from different groups of mice. F , G Biomechanical testing was performed to measure the failure load and stiffness of tendon tissues from different groups of mice. Data are presented as mean ± SD, and statistical analysis was conducted using one-way ANOVA followed by Tukey’s post hoc test. n = 6. *p < 0.05, **p < 0.01, ***p < 0.001.
Techniques Used: Staining, Derivative Assay, Immunohistochemistry, Western Blot, Expressing