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yap1 coding sequence  (Addgene inc)


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    Structured Review

    Addgene inc yap1 coding sequence
    Yap1 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yap1+coding+sequence/pcDNA+Flag+Yap1+(Plasmid+%2318881)/pm38701775-298-1-6
    Average 94 stars, based on 40 article reviews
    yap1 coding sequence - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Crk mediates Csk-Hippo signaling independently of Yap tyrosine phosphorylation to induce cell extrusion
    Article Snippet: Antibodies against Crk (BD #610036), Csk (Proteintech #17720-AP), ERK1/2 (CST #4695), pCRK (CST #3491), pERK1/2 (Santa Cruz Biotechnology, sc-7383), pSRC416 (CST #6943), pSRC527 (CST #2105), YAP (CST #14074), and phospho-tyrosine (P-Tyr-100) #9411 were used in western blot (1:1000 dilution). .. The plasmid encoding wild-type Yap was obtained from Addgene (pcDNA Flag Yap1, Addgene #18881). .. Plasmids encoding Yap 2YF and Yap 3YF were constructed from pcDNA Flag-Yap1 Y357F (Addgene #18882) through site-directed mutagenesis using Neb Q5 high-fidelity polymerase (New England Biolab #M0491).

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Article Title: AMPK stimulation inhibits YAP/TAZ signaling to ameliorate hepatic fibrosis
    Article Snippet: Briefly, 5.0 × 10^5 HSCs (per reaction) were resuspended in 100 μl of nucleofection buffer with 1 μg plasmid in a Nucleocuvette Vessel (Lonza). .. The constructs used for nucleofection: pcDNA-Flag-Yap1 (gift from Ophir Klein, Addgene plasmid #18,881), pCMV-Flag-YAP-S381A (gift from Ophir Klein, Addgene plasmid #27,377), WT AMPKα1, Kinase inactive DN mutant (gift from Kun-Liang Guan, University of California, San Diego, La Jolla, CA). .. Cells were nucleofected using the FF-113 program for P3 primary cells on the 4D-Nucleofector Core and X Unit system (Lonza) according to the manufacturer’s instructions.

    Article Title: AMPK Stimulation Inhibits YAP/TAZ Signaling to Ameliorate Hepatic Fibrosis
    Article Snippet: Brie y, 5.0 x 10^5 HSCs (per reaction) were resuspended in 100 μl of nucleofection buffer42 with 1 μg plasmid in a Nucleocuvette Vessel (Lonza). .. The constructs used for nucleofection: pcDNA-Flag-Yap1 (gift from Ophir Klein, Addgene plasmid #18881), pCMV-Flag-YAP-S381A (gift from Ophir Klein, Addgene plasmid #27377), WT AMPKα1, Kinase inactive DN mutant (gift from Kun-Liang Guan, University of California, San Diego, La Jolla, CA). .. Cells were nucleofected using the FF-113 program for P3 primary cells on the 4D-Nucleofector Core and X Unit system (Lonza) according to the manufacturer’s instructions.

    Over Expression:

    Article Title: Wild-type and mutated ß-catenin differently repress RND3/RHOE expression in hepatocellular carcinoma
    Article Snippet: .. For overexpression of YAP, plasmids were obtained from Addgene: pcDNA-Flag-Yap1 (addgene#18881; ( )) and pCMV-flag-S127A-YAP (addgene#27370; ( )) encodes respectively, WT or constitutively activated human Yap1. .. DNA transfections were performed in HepG2 cells using Lipofectamine 3000 transfection reagent (ThermoFischer) according to manufacturer’s protocol.

    Sequencing:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Construct:

    Article Title: AMPK stimulation inhibits YAP/TAZ signaling to ameliorate hepatic fibrosis
    Article Snippet: Briefly, 5.0 × 10^5 HSCs (per reaction) were resuspended in 100 μl of nucleofection buffer with 1 μg plasmid in a Nucleocuvette Vessel (Lonza). .. The constructs used for nucleofection: pcDNA-Flag-Yap1 (gift from Ophir Klein, Addgene plasmid #18,881), pCMV-Flag-YAP-S381A (gift from Ophir Klein, Addgene plasmid #27,377), WT AMPKα1, Kinase inactive DN mutant (gift from Kun-Liang Guan, University of California, San Diego, La Jolla, CA). .. Cells were nucleofected using the FF-113 program for P3 primary cells on the 4D-Nucleofector Core and X Unit system (Lonza) according to the manufacturer’s instructions.

    Article Title: AMPK Stimulation Inhibits YAP/TAZ Signaling to Ameliorate Hepatic Fibrosis
    Article Snippet: Brie y, 5.0 x 10^5 HSCs (per reaction) were resuspended in 100 μl of nucleofection buffer42 with 1 μg plasmid in a Nucleocuvette Vessel (Lonza). .. The constructs used for nucleofection: pcDNA-Flag-Yap1 (gift from Ophir Klein, Addgene plasmid #18881), pCMV-Flag-YAP-S381A (gift from Ophir Klein, Addgene plasmid #27377), WT AMPKα1, Kinase inactive DN mutant (gift from Kun-Liang Guan, University of California, San Diego, La Jolla, CA). .. Cells were nucleofected using the FF-113 program for P3 primary cells on the 4D-Nucleofector Core and X Unit system (Lonza) according to the manufacturer’s instructions.

    Mutagenesis:

    Article Title: AMPK stimulation inhibits YAP/TAZ signaling to ameliorate hepatic fibrosis
    Article Snippet: Briefly, 5.0 × 10^5 HSCs (per reaction) were resuspended in 100 μl of nucleofection buffer with 1 μg plasmid in a Nucleocuvette Vessel (Lonza). .. The constructs used for nucleofection: pcDNA-Flag-Yap1 (gift from Ophir Klein, Addgene plasmid #18,881), pCMV-Flag-YAP-S381A (gift from Ophir Klein, Addgene plasmid #27,377), WT AMPKα1, Kinase inactive DN mutant (gift from Kun-Liang Guan, University of California, San Diego, La Jolla, CA). .. Cells were nucleofected using the FF-113 program for P3 primary cells on the 4D-Nucleofector Core and X Unit system (Lonza) according to the manufacturer’s instructions.

    Article Title: AMPK Stimulation Inhibits YAP/TAZ Signaling to Ameliorate Hepatic Fibrosis
    Article Snippet: Brie y, 5.0 x 10^5 HSCs (per reaction) were resuspended in 100 μl of nucleofection buffer42 with 1 μg plasmid in a Nucleocuvette Vessel (Lonza). .. The constructs used for nucleofection: pcDNA-Flag-Yap1 (gift from Ophir Klein, Addgene plasmid #18881), pCMV-Flag-YAP-S381A (gift from Ophir Klein, Addgene plasmid #27377), WT AMPKα1, Kinase inactive DN mutant (gift from Kun-Liang Guan, University of California, San Diego, La Jolla, CA). .. Cells were nucleofected using the FF-113 program for P3 primary cells on the 4D-Nucleofector Core and X Unit system (Lonza) according to the manufacturer’s instructions.

    Generated:

    Article Title: A pipeline to identify TF combinatorial binding uncovers TEAD1 as an antagonist of tissue-specific transcription factors in human organogenesis
    Article Snippet: .. Enhancers were co-transfected with pcDNA3.1 (V790-20, Thermo Fisher), pcDNA3.1(+)-TEAD1 (generated by Genscript), pcDNA-Flag-YAP (18881, Addgene) ( ) and pcDNA3-bCrx (bovine Crx , a kind gift from Cheryl M. Craft) or pCMV-Gata6 ( ). .. Cells were transfected with GeneJuice Transfection Reagent (Novagen) 24 hours after seeding, using 250ng of enhancer plasmids and a total of 300ng of expression plasmids per well.

    CRAfT Assay:

    Article Title: A pipeline to identify TF combinatorial binding uncovers TEAD1 as an antagonist of tissue-specific transcription factors in human organogenesis
    Article Snippet: .. Enhancers were co-transfected with pcDNA3.1 (V790-20, Thermo Fisher), pcDNA3.1(+)-TEAD1 (generated by Genscript), pcDNA-Flag-YAP (18881, Addgene) ( ) and pcDNA3-bCrx (bovine Crx , a kind gift from Cheryl M. Craft) or pCMV-Gata6 ( ). .. Cells were transfected with GeneJuice Transfection Reagent (Novagen) 24 hours after seeding, using 250ng of enhancer plasmids and a total of 300ng of expression plasmids per well.



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