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vecta shield hardset mounting medium with dapi  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vecta shield hardset mounting medium with dapi
    Vecta Shield Hardset Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 3107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+shield+dapi/VECTASHIELD+Hardset+Antifade+Mounting+Medium+with+DAPI/pm37002196-646-22-28
    Average 96 stars, based on 3107 article reviews
    vecta shield hardset mounting medium with dapi - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Inhibition of pancreatic cancer growth by an siRNA targeting SNORA22 and a folic acid-conjugated cationic oligopeptide
    Article Snippet: .. This solution was added to each well, followed by a 48-h incubation with the addition of 1 mL of fresh DMEM at 24 h. Cells were then fixed with 4% paraformaldehyde, followed by immunostaining using an anti-folate receptor 1 (FOLR1) antibody (MAB5646, R&D Systems, Minneapolis, MN, USA) and DAPI staining (HardSet Mounting Medium with DAPI, Vector Laboratories, Burlingame, CA, USA). .. Each specimen was visualized using a BZ-X800 microscope (Keyence, Osaka, Japan).

    Immunostaining:

    Article Title: Inhibition of pancreatic cancer growth by an siRNA targeting SNORA22 and a folic acid-conjugated cationic oligopeptide
    Article Snippet: .. This solution was added to each well, followed by a 48-h incubation with the addition of 1 mL of fresh DMEM at 24 h. Cells were then fixed with 4% paraformaldehyde, followed by immunostaining using an anti-folate receptor 1 (FOLR1) antibody (MAB5646, R&D Systems, Minneapolis, MN, USA) and DAPI staining (HardSet Mounting Medium with DAPI, Vector Laboratories, Burlingame, CA, USA). .. Each specimen was visualized using a BZ-X800 microscope (Keyence, Osaka, Japan).

    Staining:

    Article Title: Inhibition of pancreatic cancer growth by an siRNA targeting SNORA22 and a folic acid-conjugated cationic oligopeptide
    Article Snippet: .. This solution was added to each well, followed by a 48-h incubation with the addition of 1 mL of fresh DMEM at 24 h. Cells were then fixed with 4% paraformaldehyde, followed by immunostaining using an anti-folate receptor 1 (FOLR1) antibody (MAB5646, R&D Systems, Minneapolis, MN, USA) and DAPI staining (HardSet Mounting Medium with DAPI, Vector Laboratories, Burlingame, CA, USA). .. Each specimen was visualized using a BZ-X800 microscope (Keyence, Osaka, Japan).

    Recombinant:

    Article Title: Disease-causing helix-8 mutations reprogram parathyroid hormone receptor-1 effector selectivity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody (Horseradish peroxidase (HRP)-conjugated) Santa Cruz Biotechnology, Inc Cat# sc-7392 RRID: AB_627809 Chemicals, peptides, and recombinant proteins PTH(1–34) MGH peptide core facility N/A Trp1-M-PTH(1–28) MGH peptide core facility N/A LA-PTH MGH peptide core facility N/A PTH(1–34)TMR MGH peptide core facility N/A PTH(1–34)d7 Gellman Lab N/A DMEM Gibco Cat# 11965-092 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipofectamine 2000 ThermoFisher Cat# 11668019 Opti-MEM Gibco Cat# 31985070 CO2-independent media ThermoFisher Cat# 18045088 Hanks buffer Gibco Cat# 14065-056 Bovine serum albumin Sigma Cat# A8412 D-luciferin Biotium Cat# 10101 HEPES Gibco Cat# 15630-080 Furimazine Targetmol Chem Cat# 1374040-24 Paraformaldehyde buffer Boston BioProducts Cat# BM-158 Chemiluminescent HRP substrate SuperSignal Pico via ThermoFisher Cat# 37070 Vectashield media Vector Laboratories Cat# H1500 Critical commercial assays HTRF IP-One Gq Detection Kit Revvity Cat# 62IPAPEB Deposited data PTH1R crystal structure Ehrenmann et al.69 PDB: 6FJ3 LA-PTH/PTH1R/Gαs cryo-EM structure Zhao et al.70 PDB: 6NBF PTH/PTH1R/Gαq cryo-EM structure Sano et al.71 PDB: 9JR2 PTH/PTH1R/βarrestin1 model Aydin et al.17 PDB: 9A3I NTS1/GRK2 cryo-EM structure Duan et al.33 PDB: 8JPB WT-PTH1R/Gαs complex simulations (3 × 1 μs) Redfern-Nichols et al.56 https://www.gpcrmd.org/dynadb/ dynamics/id/1296/ E465K-PTH1R/Gαs complex simulations (3 × 1μs) This study https://gpcrmd.org/dynadb/publications/ 2603/ E465K-PTH1R/Gαq complex simulations (3 × 1μs) This study https://gpcrmd.org/dynadb/publications/ 2603/ PTH/PTH1R/GRK2 complex simulations (3 × 1μs) This study https://gpcrmd.org/dynadb/publications/ 2603/ PTH/E469K-PTH1R/GRK2 complex simulations (3 × 1 μs) This study https://gpcrmd.org/dynadb/publications/ 2603/ Experimental models: Cell lines HEK293 cells ATCC Cat# CRL-1573 RRID:CVCL_0045 Gs22a cells Maeda et al.72 N/A GBR-24 cells Cheloha et al.73 N/A (Continued on next page) ll OPEN ACCESS Article e1 Cell Chemical Biology 34, 1–17.e1–e5, January 21, 2027 .. REAGENT or RESOURCE SOURCE IDENTIFIER HGO24 cells Höppner et al.28 N/A GSG5 cells Biebermann et al.74 N/A βarrestinDKO cells O’Hayre et al.29 N/A GRKKO cells Drube et al.75 N/A Gq KO cells Roszko et al.76 N/A GloResponse CRE-luc2P HEK293 Cell Line Promega Cat# E8500 Experimental models: Organisms/strains humanized PTH1R mice (hPTH1R) Daley et al.77 N/A E469K-hPTH1R mice Monahan et al.6 N/A Recombinant DNA pcDNA3.1 vector Genscript Inc. N/A WT-HA-PTH1R (pcDNA3.1) Portales-Castillo78 N/A E465K-HA-PTH1R (pcDNA3.1) Monahan et al.6 N/A E469K-HA-PTH1R (pcDNA3.1) Monahan et al.6 N/A PD-HA-PTH1R (pcDNA3.1) Höppner et al.28 N/A E469K-PD-HA-PTH1R (pcDNA3.1) This study N/A R214E-HA-PTH1R (pcDNA3.1) This study N/A R219E-HA-PTH1R (pcDNA3.1) This study N/A K472A-HA-PTH1R (pcDNA3.1) This study N/A pGlosensor-22F (pcDNA3.1) Promega Cat# E1290 Gs-OneGO (pLVX-CMV) Janicot et al.19 N/A Gq-OneGO (pLVX-CMV) Janicot et al.19 N/A smBiT-mini-Gαs (pCAGGS) This study N/A smBiT-mini-Gαq (pCAGGS) This study N/A smBiT-Gβ1 (pCAGGS) Inoue et al.79 N/A smBiT-βarrestin2 (pCAGGS) Xu et al.80 N/A smBiT-Asp67Lys-βarrestin2 (pCAGGS) This study N/A smBiT-dFLR-βarrestin2 (pCAGGS) This study N/A smBiT-GRK2 (pCAGGS) Kawakami et al.81 N/A LgBiT-CAAX (pCAGGS) Xu et al.80 N/A WT-PTH1R-LgBiT (pCAGGS) This study N/A βarrestin2 (pcDNA3.1) Höppner et al.28 N/A Asp67Lys-βarrestin2 (pcDNA3.1) This study N/A dFLR-βarrestin2 (pcDNA3.1) Höppner et al.28 N/A βarrestin2YFP (pcDNA3.1) Feinstein et al.82 N/A Software and algorithms Word for Mac Microsoft https://www.microsoft.com/en-us/ microsoft-365/mac/microsoft-365-for-mac Excel for Mac Microsoft https://www.microsoft.com/en-us/ microsoft-365/mac/microsoft-365-for-mac Affinity Publisher 2 Pantone https://www.affinity.studio/page-layout- software GraphPad Prism version 10 for Mac GraphPad Software https://www.graphpad.com Molecular Operating Environment (MOE) software Chemical Computing Group https://www.chemcomp.com/en/Products.



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