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dab immpact substrate chromogen solution  (Vector Laboratories)


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    Vector Laboratories dab immpact substrate chromogen solution
    Dab Immpact Substrate Chromogen Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2988 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+red+chromogen+solution/ImmPACT+DAB+Peroxidase+(HRP)+Substrate/pmc03537726-78-16-21
    Average 96 stars, based on 2988 article reviews
    dab immpact substrate chromogen solution - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Binding Assay:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Nonspecific Binding:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Blocking Assay:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Staining:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Polymer:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Plasmid Preparation:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Activity Assay:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Incubation:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Control:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Negative Control:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Microscopy:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Fluorescence Microscopy:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Fluorescence:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used

    Passaging:

    Article Title: Non-Clinical Evaluation of the Anti-PD-1 Antibody UDIZ-007
    Article Snippet: body (Bio-Rad, rabbit, Cat. No. 4510-7804, Kidlington, UK) at 2.0 μg/mL, followed by an anti-rabbit HRP-conjugated polymer (Abcam, goat, code ab214880, Cambridge, UK) and developed with DAB chromogen (Vector Labs, ImmPACT DAB, Cat. No. SK-4105, Newark, CA, USA). Normal goat serum (Vector Labs, code S-1000-20, Newark, CA, USA) and 10% casein (Vector Labs, Cat. No. SP-5020, Newark, CA, USA) were used



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    Image Search Results


    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Journal: Theranostics

    Article Title: Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors

    doi: 10.7150/thno.11711

    Figure Lengend Snippet: Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Article Snippet: The chromogenic alkaline phosphatase solution (Vector Labs, Cat #SK-5100) was prepared according to the manufacturer's instruction and added to the slides following 1 h staining of secondary anti-biotin antibody (Vector Labs, Cat #SP-3020) (1: 250 dilution).

    Techniques: Double Staining, Staining, Injection