hepes (Thermo Fisher)

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Thermo Fisher hepes
Hepes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tyrode+buffer+solution/Tyrode's+Solution%2C+HEPES-buffered/pmc12995707-85-2-4
Average 94 stars, based on 1 article reviews

hepes - by Bioz Stars, 2026-09

94/100 stars

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Dispersion:

Article Title: Controlling PEDOT:PSS electropolymerization for label-free optical recording of bioelectric potentials.
Article Snippet: For spin-coated PEDOT:PSS films, a dispersion consisting of 16 mL PEDOT:PSS (Clevios PH1000, Heraeus), 4 mL ethylene glycol (Sigma-Aldrich), 24 mL sodium dodecylbenzenesulfonate (Tokyo Chemical Industry Co., Ltd), and 200 mL (3-glycidyloxypropyl)trimethoxysilane (Sigma-Aldrich) was prepared by vortex mixing followed by sonication for 5 min and filtration through a 1.2 mm cellulose acetate syringe filter (add vendor here). .. The dispersion was spin-coated onto ITO substrates at 5500 rpm using a static dispense method and annealed at 140 1C for at least 1 h. Prior to measurements, all spin-coated films were electrochemically pre-conditioned by cycling the potential between 0.3 V and 0.3 V at a scan rate of 100 mV s 1 for 5 cycles in HEPES (4-(2-hydroxyethyl) 1-piperazineethanesulfonic acid)-buffered Tyrode’s solution (1 mL 1 M CaCl2, 500 mL 1 M MgCl2, 5 mL HEPES stock (1 M) (Gibco, Thermo Fisher), 4.237 g NaCl (145 mM), 149 mg KCl (4 mM), 500 mL Milli-Q water. ..

Dissection:

Article Title: c-Abl controls early embryonic morphogenesis through YAP/p73-dependent regulation of trophectoderm and inner cell mass lineages
Article Snippet: Zygotes were collected from the oviducts 20 h after mating and cultured in KSOM + AA medium (Sigma, #MR-106-D) under 5% CO2 and 5% O2 at 37 °C [ ]. .. Oviduct dissection and embryo retrieval were performed in HEPES-buffered medium (Thermo, #12,340,030) pre-warmed to 37 °C. .. Embryo culture medium was changed every two days, and embryos were monitored from the zygote to the blastocyst stage using a Zeiss Axio Observer microscope for morphological evaluation.

Cell Culture:

Article Title: Theaflavin-3,3'-digallate improves porcine oocyte maturation and independently enhances embryo development in vitro via modulating redox homeostasis and lipid-associated metabolism.
Article Snippet: Oxidative stress and metabolic imbalance are major limiting factors for oocyte maturation and early embryonic development under in vitro conditions.. Theaflavin-3,3′-digallate (TFDG), a natural polyphenolic compound derived from black tea, exhibits potent antioxidant and lipid-regulatory activities.. However, its potential role in mammalian embryo development remains unclear.

Staining:

Article Title: Induction of Moderate DNA Damage Enhances Megakaryopoiesis and Platelet Production.
Article Snippet: .. Flow cytometric analysis of murine platelets in whole blood After terminal exsanguination into EDTA-coated tubes, whole blood (5 μL) was diluted in 500 μL of Tyrode’s buffer (J67607, Alfa Aesar) and stained with thiazole orange (TO; 200 ng/mL) for 15 min. Flow cytometric analysis (Accuri C6 plus, BD Biosciences) was performed to identify the frequency of TO(+) platelets. ..

Recombinant:

Article Title: Zinc eluted from glassware is a risk factor for embryo development in human and animal assisted reproduction†.
Article Snippet: .. COCs were aspirated from small follicles (2–6 mm in diameter) and placed in 25 mM HEPES-buffered TCM199 (Gibco M199, 12340-030; ThermoFisher Scientific, Waltham, MA, USA) with 5% new born calf serum (S0750; Biowest, Nuaillé, France) and 0.1 IU/ml recombinant human folliclestimulating hormone (Follistim; MSD, Tokyo, Japan). .. Subsequently, the COCs in tubes (20–40 COCs / 500 μl medium under 300 μl liquid paraffin in 1.5 ml plastic tubes) were transferred to a portable incubator (01213900; Fujihira Industry Co., Ltd., Tokyo, Japan) and cultured at 38.5◦C during transport.

Incubation:

Article Title: Paclitaxel activates SOCE/I CRAC in dorsal root ganglion neurons: implications for paclitaxel-induced peripheral neuropathy.
Article Snippet: Measurement of intracellular calcium concentration [Ca2+]i The intracellular calcium concentration [Ca2+]i was measured in single cells by live-cell ratiometric imaging experiments using a Leica DMI6000 epifluorescence microscope equipped with S Fluor x40/1.3 objective. .. DRG cells, previously plated on 24 mm cover-slips, were incubated with 5 μM Fura 2-AM (Life Technologies, Italy) fluorescent probe, 10 μM sulfinpyrazone (Sigma Adrich Inc., Italy) and 0.02 % of Pluronic-127 (Life Technologies, Italy) in Tyrode standard solution (TS) for 20 min at room temperature. ..

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