trypleselect solution (Lonza)

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Lonza trypleselect solution
Trypleselect Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypleselect+solution/tryple+select+solution/pm24585677-72-16-18
Average 90 stars, based on 1 article reviews

trypleselect solution - by Bioz Stars, 2026-11

90/100 stars

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Article Title: Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells
Article Snippet: After the co-culture, the supernatant was removed, the cell layer washed with DPBS (Lonza, Belgium), and the adherent cells were harvested after detachment with TrypLE Select solution (Lonza, Belgum) for 10 min at 37 °C and then washed in DPBS by being centrifuged at 1200× g for 10 min.

Article Title: Age-related changes in human bone marrow mesenchymal stromal cells: morphology, gene expression profile, immunomodulatory activity and miRNA expression.
Article Snippet: Thereafter, cells were detached with TrypleSelect solution (Lonza) and subcultured at 103 cells/cm2 for their expansion by passage.

Article Title: Age-related changes in human bone marrow mesenchymal stromal cells: morphology, gene expression profile, immunomodulatory activity and miRNA expression
Article Snippet: Thereafter, cells were detached with TrypleSelect solution (Lonza) and subcultured at 10 3 cells/cm 2 for their expansion by passage.

Article Title: Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells
Article Snippet: When sub-confluence was reached, adherent cells were harvested after detachment with TrypLE Select solution (Lonza, Belgium) for 10 min at 37 °C and then washed in DPBS by being centrifuged at 1200× g for 10 min.

Article Title: Advanced Glycation End-Products-, C-Type Lectin- and Cysteinyl/ Leukotriene-Receptors in Distinct Mesenchymal Stromal Cell Populations: Differential Transcriptional Profiles in Response to Inflammation
Article Snippet: When sub-confluency (80-90%) was achieved, adherent cells were harvested by TrypLE Select solution (Lonza Belgium) and then expanded at a lower density (1,000 cells/c).

Control:

Article Title: Extracellular vesicles from chronic lymphocytic leukemia cells promote leukemia aggressiveness by inducing the differentiation of monocytes into nurse‐like cells via an RNA‐dependent mechanism
Article Snippet: The phagocytosis assay was performed with pHrodoTM Red Zymosan BioParticlesTM Conjugate (Thermo Fisher Scientific) according to the manufacturer’s recommendations. .. Briefly, 105 untreated monocytes and monocytes treated with control CLL-EVs or BCR EVs for 5 days were detached with TrypleSelect solution (Lonza), washed and resuspended in PBS supplemented with 10 mM glucose. ..

Centrifugation:

Article Title: Umbilical Cord Mesenchymal Stromal/Stem Cells and Their Interplay with Th-17 Cell Response Pathway.
Article Snippet: .. Adherent cells were harvested with TrypLE Select (Lonza) solution, washed by centrifugation in PBS (Lonza), and finally resuspended in Miltenyi Biotec buffer. ..

Incubation:

Article Title: Mammalian muscle-derived stem cells
Article Snippet: .. At subconfluence, the cells were harvested after detachment by 10 min incubation with TrypLE Select solution (Lonza). .. For passages, 5×104 cells were replated in 75 cm2 flask (Falcon) in the same culture conditions until subconfluence.

Review