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transscript reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa transscript reverse transcriptase
    Transscript Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transscript+kit/PrimeScript+FAST+RT+reagent+Kit+with+gDNA+Eraser/pm41892025-54-22-26
    Average 99 stars, based on 486 article reviews
    transscript reverse transcriptase - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Polyomavirus Drives Cerebellar Lesions in AIDS Monkeys With Ataxia
    Article Snippet: Genomic DNA was purified from tissues, SH‐SY5Y cells, and culture supernatants using a TIANamp Genomic DNA Kit (DP304, TIANGEN, China). .. RNA was reverse transcribed into cDNA using a PrimeScript FAST RT Reagent Kit with gDNA Eraser (RR092A, Takara, Japan). .. Nucleic acid concentrations were quantified with a NanoDrop ND2000 spectrophotometer (Thermo, USA).

    Article Title: An m 7 G tRNA-derived fragment mediated thermogenic activation of brown adipose tissue through post-transcriptional repression of Uqcr10.
    Article Snippet: Small RNAs were reverse-transcribed 595 using stem-loop RT primers according to the Mir-X miRNA First-Strand Synthesis Kit 596 protocol (Takara Bio, Beijing, China). .. mRNAs were reverse-transcribed with the PrimeScript 597 FAST RT reagent Kit including gDNA Eraser (Takara Bio) following the manufacturer’s 598 instructions. .. qPCR was performed with SYBR Green Master Mix (Takara Bio) on a real-time 599 instrument.

    Synthesized:

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP). .. The experiment was conducted in the ABI 7900 system (Applied Biosystems, Foster City, CA) employing the SYBR Green I Master Mix kit (Invitrogen, Waltham, MA).

    Expressing:

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP). .. The experiment was conducted in the ABI 7900 system (Applied Biosystems, Foster City, CA) employing the SYBR Green I Master Mix kit (Invitrogen, Waltham, MA).

    Differentiation Assay:

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP). .. The experiment was conducted in the ABI 7900 system (Applied Biosystems, Foster City, CA) employing the SYBR Green I Master Mix kit (Invitrogen, Waltham, MA).

    Marker:

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP). .. The experiment was conducted in the ABI 7900 system (Applied Biosystems, Foster City, CA) employing the SYBR Green I Master Mix kit (Invitrogen, Waltham, MA).

    ALP Assay:

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP). .. The experiment was conducted in the ABI 7900 system (Applied Biosystems, Foster City, CA) employing the SYBR Green I Master Mix kit (Invitrogen, Waltham, MA).



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