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Beijing TransGen Biotech transscript reverse transcriptase kit at-101
Transscript Reverse Transcriptase Kit At 101, supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transscript%C2%AE+reverse+transcriptase+kit/transscript+reverse+transcriptase+kit+at+101/pm30816441-152-6-11
Average 90 stars, based on 1 article reviews
transscript reverse transcriptase kit at-101 - by Bioz Stars, 2026-09
90/100 stars

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Reverse Transcription:

Article Title: MicroRNA let‑7d‑5p rescues ovarian cancer cell apoptosis and restores chemosensitivity by regulating the p53 signaling pathway via HMGA1.
Article Snippet: Nano-Drop (ND-1000) spectrophotometry and 1% agarose gel electrophoresis were used to measure RNA concentration, integrity and purity. .. According to the protocols of the TransScript Reverse Transcriptase kit (AT-101; Beijing TransGen Biotech Co., Ltd., Beijing, China), reverse transcription was conducted in a PCR amplification instrument to synthesize cDNA templates. .. The designed let-7d-5p, HMGA1, p53 wild-type (p53wt), U6 and GAPDH were all synthesized by Beijing Genomics Institute Co., Ltd. (Shenzhen, Guangdong, China; Table I).

Article Title: Knockout of SIRT4 decreases chemosensitivity to 5-FU in colorectal cancer cells.
Article Snippet: Total RNA was isolated from tissues and cells with TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol. .. RNA was reverse-transcribed into cDNA using TransScript Reverse Transcriptase (AT101-02; Beijing Transgen Biotech Co., Ltd., Beijing, China). qPCR was performed using the SYBR Green PCR Mix (AQ141-01; Beijing Transgen Biotech, Beijing, China) and the Bio-Rad CFX96TM Real-Time PCR system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..

Polymerase Chain Reaction:

Article Title: MicroRNA let‑7d‑5p rescues ovarian cancer cell apoptosis and restores chemosensitivity by regulating the p53 signaling pathway via HMGA1.
Article Snippet: Nano-Drop (ND-1000) spectrophotometry and 1% agarose gel electrophoresis were used to measure RNA concentration, integrity and purity. .. According to the protocols of the TransScript Reverse Transcriptase kit (AT-101; Beijing TransGen Biotech Co., Ltd., Beijing, China), reverse transcription was conducted in a PCR amplification instrument to synthesize cDNA templates. .. The designed let-7d-5p, HMGA1, p53 wild-type (p53wt), U6 and GAPDH were all synthesized by Beijing Genomics Institute Co., Ltd. (Shenzhen, Guangdong, China; Table I).

Article Title: Knockout of SIRT4 decreases chemosensitivity to 5-FU in colorectal cancer cells.
Article Snippet: Total RNA was isolated from tissues and cells with TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol. .. RNA was reverse-transcribed into cDNA using TransScript Reverse Transcriptase (AT101-02; Beijing Transgen Biotech Co., Ltd., Beijing, China). qPCR was performed using the SYBR Green PCR Mix (AQ141-01; Beijing Transgen Biotech, Beijing, China) and the Bio-Rad CFX96TM Real-Time PCR system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..

Amplification:

Article Title: MicroRNA let‑7d‑5p rescues ovarian cancer cell apoptosis and restores chemosensitivity by regulating the p53 signaling pathway via HMGA1.
Article Snippet: Nano-Drop (ND-1000) spectrophotometry and 1% agarose gel electrophoresis were used to measure RNA concentration, integrity and purity. .. According to the protocols of the TransScript Reverse Transcriptase kit (AT-101; Beijing TransGen Biotech Co., Ltd., Beijing, China), reverse transcription was conducted in a PCR amplification instrument to synthesize cDNA templates. .. The designed let-7d-5p, HMGA1, p53 wild-type (p53wt), U6 and GAPDH were all synthesized by Beijing Genomics Institute Co., Ltd. (Shenzhen, Guangdong, China; Table I).

Real-time Polymerase Chain Reaction:

Article Title: Knockout of SIRT4 decreases chemosensitivity to 5-FU in colorectal cancer cells.
Article Snippet: Total RNA was isolated from tissues and cells with TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol. .. RNA was reverse-transcribed into cDNA using TransScript Reverse Transcriptase (AT101-02; Beijing Transgen Biotech Co., Ltd., Beijing, China). qPCR was performed using the SYBR Green PCR Mix (AQ141-01; Beijing Transgen Biotech, Beijing, China) and the Bio-Rad CFX96TM Real-Time PCR system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..

SYBR Green Assay:

Article Title: Knockout of SIRT4 decreases chemosensitivity to 5-FU in colorectal cancer cells.
Article Snippet: Total RNA was isolated from tissues and cells with TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol. .. RNA was reverse-transcribed into cDNA using TransScript Reverse Transcriptase (AT101-02; Beijing Transgen Biotech Co., Ltd., Beijing, China). qPCR was performed using the SYBR Green PCR Mix (AQ141-01; Beijing Transgen Biotech, Beijing, China) and the Bio-Rad CFX96TM Real-Time PCR system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..



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