strand specific transcriptome library preparation (Novogene)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Novogene strand specific transcriptome library preparation
Strand Specific Transcriptome Library Preparation, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptomic+libraries/library+preparation+specific+strand+transcriptome/pmc12787643-361-0-7
Average 86 stars, based on 1 article reviews

strand specific transcriptome library preparation - by Bioz Stars, 2026-10

86/100 stars

Images

Construct:

Article Title: The microbial tryptophan metabolite indole acts on the gastrointestinal tract to improve glucose homeostasis in a mouse model of diabetes by enhancing GLP-1 secretion and L cell differentiation.
Article Snippet: RNA sequencing Purified total RNA samples were analysed using Nanodrop (A260/A280 >1.8, suggesting good RNA purity), and RNA integrity was checked using an Agilent Bioanalyzer RNA 6000 Nano assay (RNA integrity number [RIN] >7). .. Strand-specific mRNA (polyA) libraries were constructed at Novogene UK, and sequenced using an Illumina NovaSeq 6000 sequencing system with 5.8–7.9 billion bases per sample. .. Adapters were trimmed using Novogene’s bioinformatics pipeline, and alignments were performed using HISAT2 [18] and mouse genome GRCm38 Ensembl version 102.

Sequencing:

Article Title: Genotype‐Dependent Transcriptome Divergence Associated With Variation at vgll3 in Juvenile Gilthead Seabream ( Sparus aurata )
Article Snippet: RNA quality and integrity were confirmed by electrophoresis in 1.5% agarose gels and spectrophotometric quantification (Nanodrop 2000, Thermo Fisher Scientific, Waltham, USA). .. The RNA samples were outsourced (Novogene, Europe, UK) for strand‐specific mRNA library preparation using poly‐A selection, followed by paired‐end sequencing (150‐bp reads) on the Illumina NovaSeq platforms. ..

Article Title: Two LEAFY homologues regulate floral patterning and development without affecting flowering time in kiwifruit.
Article Snippet: 14 For RNA-seq, the quantity and quality of RNA samples were checked using a Bioanalyzer 2100 15 (Agilent Technologies, USA) with the RIN score above 7. .. RNA was sent to Novogene (Hong 16 Kong) for directional strand-specific mRNA library preparation and NovaSeq 6000 PE150 17 sequencing. ..

Article Title: Genotype-Dependent Transcriptome Divergence Associated With Variation at vgll3 in Juvenile Gilthead Seabream (Sparus aurata).
Article Snippet: RNA quality and integrity were confirmed by electrophoresis in 1.5% agarose gels and spectrophotometric quantification (Nanodrop 2000, Thermo Fisher Scientific, Waltham, USA). .. The RNA samples were outsourced (Novogene, Europe, UK) for strand- specific mRNA library preparation using poly- A selection, followed by paired- end sequencing (150- bp reads) on the Illumina NovaSeq platforms. ..

cDNA Synthesis:

Article Title: Comparative Analysis of Insect Resistance in Transgenic Populus × euramericana cv. Neva Expressing Dual Bt Genes from Different Sources
Article Snippet: RNA quality was verified via NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), with an RNA integrity number (RIN) ≥ 7.0 required for library construction [ ]. .. Strand-specific transcriptome library preparation was performed by Novogene (Beijing, China) following their standard protocol for insect samples [ , ], which included mRNA enrichment, fragmentation, cDNA synthesis, end repair, adapter ligation, size selection, and PCR amplification. .. The qualified library was sequenced on an Illumina NovaSeq 6000 platform to generate paired-end reads [ ].

Adapter Ligation:

Size Selection:

Polymerase Chain Reaction:

Amplification:

Selection:

RNA Sequencing:

Article Title: Hyperuricemia aggravates acute pancreatitis through CNR1-mediated inflammatory signaling and gut-pancreas axis dysregulation: a multi-omics and clinical study.
Article Snippet: RNA quality was assessed using the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA) to ensure integrity and purity. .. Strand-specific RNA-seq libraries were prepared by Novogene Co., Ltd. (Beijing, China) following standard protocols. .. The clustering of indexcoded samples was performed on the cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina, CA, USA) according to the manufacturer's guidelines.

Article Title: FACT depletion results in a temporal cascade of chromatin disruption preceding transcriptional collapse in stem cells
Article Snippet: .. Purified steady-state RNA samples were used as an input for strand specific RNA-seq library builds using Novogene. .. Libraries were pooled and paired end sequencing was performed using Illumina Novaseq platform to a depth of ∼30 million reads.

RNA sequencing:

Article Title: Metabolic stress reveals widespread accumulation of cap-unmethylated RNAs
Article Snippet: 300 ng poly(A)+ RNA was added to the beads in 200 μL RIP buffer with 0.1U/μL SUPERase·In (ThermoFisher, AM2696), and incubated at 4°C overnight with rotation. .. The RNA pellet was resuspended in 20 μL water and two biological replicates were subjected to strand-specific RNA-sequencing with Novogene. ..

Review