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rna-seq library preparation kit whole transcriptome discovery  (Gnomegen Inc)

 
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    Gnomegen Inc rna-seq library preparation kit whole transcriptome discovery
    Rna Seq Library Preparation Kit Whole Transcriptome Discovery, supplied by Gnomegen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptomic+libraries/rna+seq+ngs+library+preparation+kit+for+whole+transcriptome+discovery/pmc05013307-333-19-22
    Average 90 stars, based on 1 article reviews
    rna-seq library preparation kit whole transcriptome discovery - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: The genomic basis for colonizing the freezing Southern Ocean revealed by Antarctic toothfish and Patagonian robalo genomes
    Article Snippet: 587 An integrated protocol from the Mate-Pair Library v2 Sample Preparation Guide (Illumina) 588 and the Paired-End Library Preparation Method Manual (Roche) was used to prepare 589 mate-pair libraries with insert sizes of 3, 6, 10, 15 and 20 kb (Additional file 1: Tables S1b, 590 S1c).

    Article Title: The genomic basis for colonizing the freezing Southern Ocean revealed by Antarctic toothfish and Patagonian robalo genomes
    Article Snippet: Paired-end cDNA libraries were constructed 566 using the RNA-Seq NGS Library Preparation Kit for Whole-Transcriptome Discovery 567 (Gnomegen).

    Article Title: A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat.
    Article Snippet: Paired-end cDNA libraries were constructed using the RNA-Seq NGS Library Preparation Kit for Whole-Transcriptome Discovery (Gnomegen) with 10 cycles of PCR amplification.

    cDNA Library Assay:

    Article Title: Promoter engineering for enhanced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production in Haloferax mediterranei.
    Article Snippet: .. The dRNA-seq cDNA library was then constructed using an RNA-seq Library Preparation Kit for Transcriptome Discovery (Gnomegen, CA, USA). .. In addition, the NEBNext Ultra TM Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) was used to construct standard strand-specific whole-transcript sequencing cDNA libraries. cDNA libraries for TSS mapping were sequenced on an Illumina HiSeq 2000 platform.

    Construct:

    Article Title: Promoter engineering for enhanced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production in Haloferax mediterranei.
    Article Snippet: .. The dRNA-seq cDNA library was then constructed using an RNA-seq Library Preparation Kit for Transcriptome Discovery (Gnomegen, CA, USA). .. In addition, the NEBNext Ultra TM Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) was used to construct standard strand-specific whole-transcript sequencing cDNA libraries. cDNA libraries for TSS mapping were sequenced on an Illumina HiSeq 2000 platform.

    Article Title: Nitric oxide synthase is required for the pea aphid's defence against bacterial infection.
    Article Snippet: Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Taigu, China Department of Entomology, College of Plant Protection, Northwest A & F University, Yangling, China Department of Plant Protection, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess, Ministry of Agriculture, Northwest A & F University, Yangling, China

    Article Title: The genomic basis for colonizing the freezing Southern Ocean revealed by Antarctic toothfish and Patagonian robalo genomes
    Article Snippet: For the transcriptome sequencing, Poly(A)+ messenger RNA (mRNA) was purified using the DynaBeads mRNA Purification kit (Life Technologies, Carlsbad, CA). .. Paired-end complementary DNA libraries were constructed using the RNA sequencing (RNA-Seq) Next-Generation Sequencing Library Preparation Kit for Whole-Transcriptome Discovery (Gnomegen, San Diego, CA). .. All of the libraries are sequenced on an Illumina HiSeq 1500 sequencer.

    RNA Sequencing:

    Article Title: Promoter engineering for enhanced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production in Haloferax mediterranei.
    Article Snippet: .. The dRNA-seq cDNA library was then constructed using an RNA-seq Library Preparation Kit for Transcriptome Discovery (Gnomegen, CA, USA). .. In addition, the NEBNext Ultra TM Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) was used to construct standard strand-specific whole-transcript sequencing cDNA libraries. cDNA libraries for TSS mapping were sequenced on an Illumina HiSeq 2000 platform.

    Article Title: Epithelial-mesenchymal transition markers screened in a cell-based model and validated in lung adenocarcinoma
    Article Snippet: .. Libraries were prepared using RNA-seq Library Preparation Kit for Whole Transcriptome Discovery (Gnomegen), and Balancer NGS Library Preparation Kit for small/microRNA (GnomeGen) following manufacture’s instruction. .. The libraries were applied to illumina NextSeq 500 system for 151 nt pair-end sequencing by ABlife Inc. (Wuhan, China).

    Article Title: Nitric oxide synthase is required for the pea aphid's defence against bacterial infection.
    Article Snippet: Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Taigu, China Department of Entomology, College of Plant Protection, Northwest A & F University, Yangling, China Department of Plant Protection, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess, Ministry of Agriculture, Northwest A & F University, Yangling, China

    Article Title: Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
    Article Snippet: The concentration and the integrity of the RNA samples were measured by Nanodrop ND-2000 spectrophotometer (NanoDrop, United States) and Agilent 2100 BioAnalyzer (Agilent, Palo Alto, United States), respectively. .. Total RNA (10 μg) in each group was used for constructing RNA-seq libraries following the manufacturer’s instructions of RNA-Seq Library Preparation Kit for Whole Transcriptome Discovery Kit (Gnomegen, San Diego, United States). .. RNA-seq libraries were sequenced on Illumina HiSeq TM 2000 platform (Illumina, San Diego, United States).

    Article Title: The genomic basis for colonizing the freezing Southern Ocean revealed by Antarctic toothfish and Patagonian robalo genomes
    Article Snippet: For the transcriptome sequencing, Poly(A)+ messenger RNA (mRNA) was purified using the DynaBeads mRNA Purification kit (Life Technologies, Carlsbad, CA). .. Paired-end complementary DNA libraries were constructed using the RNA sequencing (RNA-Seq) Next-Generation Sequencing Library Preparation Kit for Whole-Transcriptome Discovery (Gnomegen, San Diego, CA). .. All of the libraries are sequenced on an Illumina HiSeq 1500 sequencer.

    Next-Generation Sequencing:

    Article Title: Epithelial-mesenchymal transition markers screened in a cell-based model and validated in lung adenocarcinoma
    Article Snippet: .. Libraries were prepared using RNA-seq Library Preparation Kit for Whole Transcriptome Discovery (Gnomegen), and Balancer NGS Library Preparation Kit for small/microRNA (GnomeGen) following manufacture’s instruction. .. The libraries were applied to illumina NextSeq 500 system for 151 nt pair-end sequencing by ABlife Inc. (Wuhan, China).

    Article Title: The genomic basis for colonizing the freezing Southern Ocean revealed by Antarctic toothfish and Patagonian robalo genomes
    Article Snippet: For the transcriptome sequencing, Poly(A)+ messenger RNA (mRNA) was purified using the DynaBeads mRNA Purification kit (Life Technologies, Carlsbad, CA). .. Paired-end complementary DNA libraries were constructed using the RNA sequencing (RNA-Seq) Next-Generation Sequencing Library Preparation Kit for Whole-Transcriptome Discovery (Gnomegen, San Diego, CA). .. All of the libraries are sequenced on an Illumina HiSeq 1500 sequencer.

    RNA sequencing:

    Article Title: Nitric oxide synthase is required for the pea aphid's defence against bacterial infection.
    Article Snippet: Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Taigu, China Department of Entomology, College of Plant Protection, Northwest A & F University, Yangling, China Department of Plant Protection, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess, Ministry of Agriculture, Northwest A & F University, Yangling, China

    Article Title: Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
    Article Snippet: The concentration and the integrity of the RNA samples were measured by Nanodrop ND-2000 spectrophotometer (NanoDrop, United States) and Agilent 2100 BioAnalyzer (Agilent, Palo Alto, United States), respectively. .. Total RNA (10 μg) in each group was used for constructing RNA-seq libraries following the manufacturer’s instructions of RNA-Seq Library Preparation Kit for Whole Transcriptome Discovery Kit (Gnomegen, San Diego, United States). .. RNA-seq libraries were sequenced on Illumina HiSeq TM 2000 platform (Illumina, San Diego, United States).



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    Image Search Results


    Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques: Clinical Proteomics

    Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques:

    a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation

    doi: 10.1038/s41467-026-68879-2

    Figure Lengend Snippet: a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Article Snippet: The RNA sequencing transcriptome library was generated with a TruSeq TM RNA sample preparation kit from Illumina (San Diego, CA). cDNA was synthesized via a SuperScript double-stranded cDNA synthesis kit (Invitrogen) with random hexamer primers (Illumina).

    Techniques: RNA Sequencing, Western Blot, Comparison