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rna  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co rna
    Rna, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1501 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase/HiScript+II+Reverse+Transcriptase/pm42809636-151-17-28
    Average 97 stars, based on 1501 article reviews
    rna - by Bioz Stars, 2026-10
    97/100 stars

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    Reverse Transcription:

    Article Title: Isolation, identification, and pathogenicity of Novel Duck Reovirus strain from ducklings in China.
    Article Snippet: .. Viral RNA was then reverse transcribed into complementary DNA (cDNA) with the HiScript II Reverse Transcriptase Kit (Vazyme, Nanjing, China). ..

    Article Title: Transcriptional architecture of African swine fever virus
    Article Snippet: .. Total RNA (1 μg) was purified and reverse-transcribed into cDNA via HiScript II Reverse Transcriptase (Vazyme, China) with a specific mix of primers following the manufacturer’s instructions. .. Using 0.2-2 ug of total RNA from ASFV-infected PAMs cells, reverse transcription (HiScript II Reverse Transcriptase [Vazyme, China]) was performed with strand-specific primers—forward primers for negative-strand and reverse primers for positive-strand-specific reverse transcription.

    Article Title: Revitalizing bioinert PCL: A biomimetic 3D-Printed gradient-pore scaffold with CSMA hydrogel-mediated BMP-2/QK delivery for vascularized bone regeneration.
    Article Snippet: Critical-sized bone defects exceed bone’s regenerative capacity and require space-maintaining implants that support vascular and bone-tissue ingrowth.. Three-dimensional (3D)-printed polycaprolactone (PCL) scaffolds are widely investigated for bone repair owing to controllable geometry, interconnected macropores, and structural stability.. However, uniform-pore designs cannot recapitulate heterogeneous bone porosity, while hydrophobic PCL has limited bioactivity.

    Article Title: Environmentally relevant phthalic acid exposure promotes pulmonary fibrogenesis through IL-1α/NF-κB pathway: Implications for microplastic-associated respiratory health risks.
    Article Snippet: Total RNA was isolated from cells using Trizol reagent (Invitrogen). .. Subsequently, 1 μg of RNA was reverse-transcribed into cDNA with HiScript Reverse Transcriptase (Vazyme) following the manufacturer's protocol. .. Quantitative real-time PCR (RT-qPCR) was performed in triplicate using a LightCycler 480 II system (Roche) with ChamQ Universal SYBR qPCR Master Mix (Vazyme) (Shan et al., 2021).

    Article Title: Integrating in vivo metabolism, pharmacokinetics, and dual-indicator scoring for PK-marker selection of Celosiae Semen saponins.
    Article Snippet: The total saponins from Celosiae Semen (TSCS) show hepatoprotective and lipid-lowering effects against metabolic dysfunction-associated steatotic liver disease (MASLD).. However, their in vivo metabolic fate and holistic pharmacokinetic (PK) features remain largely uncharacterized.. In this study, an integrated PK-marker screening strategy was established by combining in vivo metabolic profiling, area under the curve (AUC)weighted composite PK analysis, and a dual-indicator scoring model that weights both systemic exposure and in vitro lipid-lowering activity.

    Article Title: Precision base-editing of the cryptic 3' acceptor site to correct the RNA splicing defect of β 654 -thalassemia.
    Article Snippet: .. Total RNA was reverse transcribed using the HiScript II Reverse Transcriptase Kit (Vazyme). ..

    Article Title: Comparative evaluation of three protein nanoscaffolds for IHNV G ectodomain display in rainbow trout (Oncorhynchusmykiss).
    Article Snippet: Infectious hematopoietic necrosis virus (IHNV) causes severe losses in rainbow trout (Oncorhynchus mykiss) aquaculture.. Vaccination is a critical control strategy, and the surface glycoprotein (G) is the primary target for protective immunity.. However, recombinant G protein alone is poorly immunogenic, prompting the use of selfassembling nanoparticle scaffolds to display its ectodomain and enhance immunogenicity.

    Article Title: Anticancer evaluation of novel chiral tetrahydropyrazolo[1,5-a]pyrimidine derivatives: Inhibitory effects on proliferation and migration of colon cancer cells with high HER2 expression.
    Article Snippet: In colorectal cancer (CRC), oncogenic activation of the human epidermal growth factor receptor 2 (HER2) is strongly associated with tumor metastasis, poor prognosis, and resistance to anti-EGFR targeted therapies.. Currently, there is a lack of tyrosine kinase inhibitors (TKIs) targeting HER2-high-expressing CRC.. Current therapeutic approaches face challenges including intertumoral heterogeneity in treatment response and the rapid emergence of drug resistance.

    Purification:

    Article Title: Transcriptional architecture of African swine fever virus
    Article Snippet: .. Total RNA (1 μg) was purified and reverse-transcribed into cDNA via HiScript II Reverse Transcriptase (Vazyme, China) with a specific mix of primers following the manufacturer’s instructions. .. Using 0.2-2 ug of total RNA from ASFV-infected PAMs cells, reverse transcription (HiScript II Reverse Transcriptase [Vazyme, China]) was performed with strand-specific primers—forward primers for negative-strand and reverse primers for positive-strand-specific reverse transcription.

    RNA Extraction:

    Article Title: Integrating in vivo metabolism, pharmacokinetics, and dual-indicator scoring for PK-marker selection of Celosiae Semen saponins.
    Article Snippet: The total saponins from Celosiae Semen (TSCS) show hepatoprotective and lipid-lowering effects against metabolic dysfunction-associated steatotic liver disease (MASLD).. However, their in vivo metabolic fate and holistic pharmacokinetic (PK) features remain largely uncharacterized.. In this study, an integrated PK-marker screening strategy was established by combining in vivo metabolic profiling, area under the curve (AUC)weighted composite PK analysis, and a dual-indicator scoring model that weights both systemic exposure and in vitro lipid-lowering activity.



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    Major antiviral‐resistant viruses and key drugs used in clinical practice.

    Journal: BioMed Research International

    Article Title: Trends, Gaps, and Challenges in Antiviral Resistance: A Bibliometric Analysis and Narrative Synthesis of Key Viruses, Resistance Mechanisms, and Drivers

    doi: 10.1155/bmri/3771603

    Figure Lengend Snippet: Major antiviral‐resistant viruses and key drugs used in clinical practice.

    Article Snippet: Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) (Coronaviridae) , RNA‐dependent RNA polymerase (RdRp) inhibitors: remdesivir; oral RdRp‐targeting agent: molnupiravir; 3CL protease inhibitor: nirmatrelvir (in Paxlovid); monoclonal antibodies (e.g., earlier agents now largely inactive) , RdRp targeting: E802D, C799F, several others under selection; spike protein targeting (mAbs): many S domain mutations (e.g., E484K, N501Y related lineages that evade certain antibodies) , Potential for reduced drug susceptibility and escape from neutralizing antibodies; particular concern in immunocompromised hosts receiving prolonged therapy. , [ , ] .

    Techniques: Virus, Reverse Transcription, Transmission Assay, Protease Inhibitor, Bioprocessing, Selection