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Promega reverse transcriptase buffer
Reverse Transcriptase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+buffer/reverse+transcriptase+buffer/pmc02775543-327-16-19
Average 90 stars, based on 1 article reviews
reverse transcriptase buffer - by Bioz Stars, 2026-09
90/100 stars

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Reverse Transcription:

Article Title: Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells.
Article Snippet: .. The reaction mixture contained deoxynucleotide triphosphates, 10 × reverse transcription buffer, oligodeoxythymidylic acid, recombinant ribonuclease inhibitor, and Moloney murine leukemia virus reverse transcriptase (cat. no. U1515, A3561, C1101, N2515, and M1705, respectively, Promega, USA). ..

Article Title: Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis
Article Snippet: .. The reverse transcription reaction was conducted in a final volume of 10 μl, containing 4 μl of extracted RNA, 2 μM oligo(dT) 12-18 (Amersham Pharmacia, Orsay, France), 1X reverse transcriptase buffer (Promega, Charbonnière, France), 2.5 mM MgCl 2 (Promega), 1.25 mM of each dNTP (Promega), 20 IU of recombinant ribonuclease inhibitor (RNasin, Promega), and 100 IU of Moloney murine leukemia virus reverse transcriptase (M-MLV, Promega). ..

Article Title: Fast Maturation of Splenic Dendritic Cells Upon TBI Is Associated With FLT3/FLT3L Signaling
Article Snippet: .. The samples were placed on ice and a master mix of 0.5μl reverse transcriptase (Promega, Germany), 0.5 μl RNase Inhibitor (RiboLock, Thermo Scientific, Germany), 2 μl dNTPs (Genaxxon, Germany), and 12 μl reverse transcriptase buffer (Promega, Germany). ..

Article Title: CD300LF + microglia impede the neuroinflammation following traumatic brain injury by inhibiting STING pathway
Article Snippet: .. The samples were then cooled on ice, and a master mixture containing 0.5 μL of reverse transcriptase (Promega, Germany), 0.5 μL of RNase Inhibitor (RiboLock, Thermo Fisher Scientific, Germany), 2 μL of dNTPs (Genaxxon, Germany), and 12 μL of reverse transcriptase buffer (Promega, Germany) was added. ..

Article Title: RGC-32 Acts as a Hub to Regulate the Transcriptomic Changes Associated With Astrocyte Development and Reactive Astrocytosis.
Article Snippet: .. July 2021 | Volume 12 | Article 705308 RNA Isolation and cDNA Synthesis Total RNA from mouse astrocytes was purified using an RNeasy Mini Kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions: 0.5 mg of RNA per sample was reverse-transcribed in a mixture containing reverse transcriptase buffer (Promega, Madison, WI), dNTPs (Promega), and random primers (Invitrogen, Carlsbad, CA). .. The RNA was denatured by incubation at 65°C for 5 min. M-MLV reverse transcriptase (Promega) was then added, and the reaction mixture was incubated at 37°C for 1 h. The reaction was terminated by incubation of the mixture at 95°C for 5 min.

Article Title: Beta2 adrenergic receptor–mediated abnormal myelopoiesis drives neuroinflammation in aged patients with traumatic brain injury
Article Snippet: .. The samples were then cooled on ice, and a master mixture containing 0.5 μl of reverse transcriptase (Promega, Germany), 0.5 μl of RNase inhibitor (RiboLock, Thermo Fisher Scientific, Germany), 2 μl of deoxynucleotide triphosphates (Genaxxon, Germany), and 12 μl of reverse transcriptase buffer (Promega, Germany) was added. ..

Article Title: Fast Maturation of Splenic Dendritic Cells Upon TBI Is Associated With FLT3/FLT3L Signaling.
Article Snippet: .. The samples were placed on ice and a master mix of 0.5μl reverse transcriptase (Promega, Germany), 0.5 μl RNase Inhibitor (RiboLock, Thermo Scientific, Germany), 2 μl dNTPs (Genaxxon, Germany), and 12 μl reverse transcriptase buffer (Promega, Germany). ..

Recombinant:

Article Title: Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells.
Article Snippet: .. The reaction mixture contained deoxynucleotide triphosphates, 10 × reverse transcription buffer, oligodeoxythymidylic acid, recombinant ribonuclease inhibitor, and Moloney murine leukemia virus reverse transcriptase (cat. no. U1515, A3561, C1101, N2515, and M1705, respectively, Promega, USA). ..

Article Title: Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis
Article Snippet: .. The reverse transcription reaction was conducted in a final volume of 10 μl, containing 4 μl of extracted RNA, 2 μM oligo(dT) 12-18 (Amersham Pharmacia, Orsay, France), 1X reverse transcriptase buffer (Promega, Charbonnière, France), 2.5 mM MgCl 2 (Promega), 1.25 mM of each dNTP (Promega), 20 IU of recombinant ribonuclease inhibitor (RNasin, Promega), and 100 IU of Moloney murine leukemia virus reverse transcriptase (M-MLV, Promega). ..

Virus:

Article Title: Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells.
Article Snippet: .. The reaction mixture contained deoxynucleotide triphosphates, 10 × reverse transcription buffer, oligodeoxythymidylic acid, recombinant ribonuclease inhibitor, and Moloney murine leukemia virus reverse transcriptase (cat. no. U1515, A3561, C1101, N2515, and M1705, respectively, Promega, USA). ..

Article Title: Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis
Article Snippet: .. The reverse transcription reaction was conducted in a final volume of 10 μl, containing 4 μl of extracted RNA, 2 μM oligo(dT) 12-18 (Amersham Pharmacia, Orsay, France), 1X reverse transcriptase buffer (Promega, Charbonnière, France), 2.5 mM MgCl 2 (Promega), 1.25 mM of each dNTP (Promega), 20 IU of recombinant ribonuclease inhibitor (RNasin, Promega), and 100 IU of Moloney murine leukemia virus reverse transcriptase (M-MLV, Promega). ..

Isolation:

Article Title: RGC-32 Acts as a Hub to Regulate the Transcriptomic Changes Associated With Astrocyte Development and Reactive Astrocytosis.
Article Snippet: .. July 2021 | Volume 12 | Article 705308 RNA Isolation and cDNA Synthesis Total RNA from mouse astrocytes was purified using an RNeasy Mini Kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions: 0.5 mg of RNA per sample was reverse-transcribed in a mixture containing reverse transcriptase buffer (Promega, Madison, WI), dNTPs (Promega), and random primers (Invitrogen, Carlsbad, CA). .. The RNA was denatured by incubation at 65°C for 5 min. M-MLV reverse transcriptase (Promega) was then added, and the reaction mixture was incubated at 37°C for 1 h. The reaction was terminated by incubation of the mixture at 95°C for 5 min.

cDNA Synthesis:

Article Title: RGC-32 Acts as a Hub to Regulate the Transcriptomic Changes Associated With Astrocyte Development and Reactive Astrocytosis.
Article Snippet: .. July 2021 | Volume 12 | Article 705308 RNA Isolation and cDNA Synthesis Total RNA from mouse astrocytes was purified using an RNeasy Mini Kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions: 0.5 mg of RNA per sample was reverse-transcribed in a mixture containing reverse transcriptase buffer (Promega, Madison, WI), dNTPs (Promega), and random primers (Invitrogen, Carlsbad, CA). .. The RNA was denatured by incubation at 65°C for 5 min. M-MLV reverse transcriptase (Promega) was then added, and the reaction mixture was incubated at 37°C for 1 h. The reaction was terminated by incubation of the mixture at 95°C for 5 min.

Purification:

Article Title: RGC-32 Acts as a Hub to Regulate the Transcriptomic Changes Associated With Astrocyte Development and Reactive Astrocytosis.
Article Snippet: .. July 2021 | Volume 12 | Article 705308 RNA Isolation and cDNA Synthesis Total RNA from mouse astrocytes was purified using an RNeasy Mini Kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions: 0.5 mg of RNA per sample was reverse-transcribed in a mixture containing reverse transcriptase buffer (Promega, Madison, WI), dNTPs (Promega), and random primers (Invitrogen, Carlsbad, CA). .. The RNA was denatured by incubation at 65°C for 5 min. M-MLV reverse transcriptase (Promega) was then added, and the reaction mixture was incubated at 37°C for 1 h. The reaction was terminated by incubation of the mixture at 95°C for 5 min.

other:

Article Title: Cytokines and Neurodegeneration in Epileptogenesis
Article Snippet: The reaction mix also contained RNaze-free water, buffer for reverse transcriptase (Promega, Madison, WI, USA), dNTPs (10 mM; Applied Biosystems, Waltham, MA, USA), 9-nucleotide random primers (nonamers; Sigma, Poznan, Poland) and RNaze inhibitor (Rnasin; Promega, Madison, WI, USA) 12.5 U/1 μg RNA.



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