Review



moclo crispr cas toolkit  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc moclo crispr cas toolkit
    Synthetic biology tools for plants and algae.
    Moclo Crispr Cas Toolkit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/MoClo+CRISPR%2FCas+Toolkit+for+Plants+(Kit+%231000000159)/pmc09545776-3-2-8
    Average 93 stars, based on 2 article reviews
    moclo crispr cas toolkit - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Biocircuits in plants and eukaryotic algae"

    Article Title: Biocircuits in plants and eukaryotic algae

    Journal: Frontiers in Plant Science

    doi: 10.3389/fpls.2022.982959

    Synthetic biology tools for plants and algae.
    Figure Legend Snippet: Synthetic biology tools for plants and algae.

    Techniques Used: Algae, Transformation Assay, Construct, Translocation Assay, CRISPR, Expressing, Functional Assay, Sequencing, In Silico, Software, Bacteria, Gene Expression

    Related Articles

    CRISPR:

    Article Title: Biocircuits in plants and eukaryotic algae
    Article Snippet: , , Nicola Patron lab plasmids/Addgene ( https://www.addgene.org/Nicola_Patron/ ) , It holds more than 200 plasmids, deposited by the Nicola Patron lab, to be distributed to the scientific community, in addition to the MoClo plant parts kits. , Multiple references mentioned in Addgene table.. .. , , MoClo CRISPR/Cas toolkit for Plants ( https://www.addgene.org/kits/nekrasov-moclo-plant-crispr/ ) , Kit collection with 95 plasmids for transformation and expression of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters in plants. , . .. , , pENFRUIT gateway collection ( https://www.addgene.org/browse/article/2397/ ) , The collection comprises several functional DNA parts (fruit promoters, GOIs and terminators) ready to be assembled in a combinatorial way using Gateway system. , .

    Article Title: A User’s Guide to Golden Gate Cloning Methods and Standards
    Article Snippet: Parts for geminiviral expression vectors , Addgene plasmids 106207 – 106218 , ( ) , Parts for geminiviral expression vectors. Also an additional set of α backbones for GoldenBraid assemblies with more than 2 parts per cycle. , AmpR † , BsaI. .. MoClo CRISPR/Cas Toolkit for Plants , Addgene toolkit 1000000159 , ( ) , CRISPR/Cas nucleases and gRNA backbones. , SpeR or KanR , BsaI. .. uLoop: Universal Loop Assembly , From the corresponding author (Addgene submission pending) , ( ) , An expansion of the loop assembly toolkit including binary destination vectors for diatoms, yeast, plants, and bacteria. , SpeR , BsaI.

    Transformation Assay:

    Article Title: Biocircuits in plants and eukaryotic algae
    Article Snippet: , , Nicola Patron lab plasmids/Addgene ( https://www.addgene.org/Nicola_Patron/ ) , It holds more than 200 plasmids, deposited by the Nicola Patron lab, to be distributed to the scientific community, in addition to the MoClo plant parts kits. , Multiple references mentioned in Addgene table.. .. , , MoClo CRISPR/Cas toolkit for Plants ( https://www.addgene.org/kits/nekrasov-moclo-plant-crispr/ ) , Kit collection with 95 plasmids for transformation and expression of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters in plants. , . .. , , pENFRUIT gateway collection ( https://www.addgene.org/browse/article/2397/ ) , The collection comprises several functional DNA parts (fruit promoters, GOIs and terminators) ready to be assembled in a combinatorial way using Gateway system. , .

    Expressing:

    Article Title: Biocircuits in plants and eukaryotic algae
    Article Snippet: , , Nicola Patron lab plasmids/Addgene ( https://www.addgene.org/Nicola_Patron/ ) , It holds more than 200 plasmids, deposited by the Nicola Patron lab, to be distributed to the scientific community, in addition to the MoClo plant parts kits. , Multiple references mentioned in Addgene table.. .. , , MoClo CRISPR/Cas toolkit for Plants ( https://www.addgene.org/kits/nekrasov-moclo-plant-crispr/ ) , Kit collection with 95 plasmids for transformation and expression of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters in plants. , . .. , , pENFRUIT gateway collection ( https://www.addgene.org/browse/article/2397/ ) , The collection comprises several functional DNA parts (fruit promoters, GOIs and terminators) ready to be assembled in a combinatorial way using Gateway system. , .

    Plasmid Preparation:

    Article Title: Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes.
    Article Snippet: .. Plasmid vectors for Cas9-mediated targeted mutagenesis were constructed according to Lawrenson et al. (2015) using the Plant Modular Cloning Golden Gate toolkit, a gift from Sylvestre Marillonnet (Addgene #1000000044; Engler et al. 2014). .. Briefly, targets were introduced into single guide RNA (sgRNA) scaffolds by PCR (for primers see Table S2) and assembled with a U6 small RNA promoter from either Arabidopsis (plCSL90002; Addgene #68261) or potato (Wang et al., 2015) into a Level 1 acceptor plasmid (plCH47751, plCH47761, plCH47772 or plCH47781).

    Mutagenesis:

    Article Title: Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes.
    Article Snippet: .. Plasmid vectors for Cas9-mediated targeted mutagenesis were constructed according to Lawrenson et al. (2015) using the Plant Modular Cloning Golden Gate toolkit, a gift from Sylvestre Marillonnet (Addgene #1000000044; Engler et al. 2014). .. Briefly, targets were introduced into single guide RNA (sgRNA) scaffolds by PCR (for primers see Table S2) and assembled with a U6 small RNA promoter from either Arabidopsis (plCSL90002; Addgene #68261) or potato (Wang et al., 2015) into a Level 1 acceptor plasmid (plCH47751, plCH47761, plCH47772 or plCH47781).

    Construct:

    Article Title: Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes.
    Article Snippet: .. Plasmid vectors for Cas9-mediated targeted mutagenesis were constructed according to Lawrenson et al. (2015) using the Plant Modular Cloning Golden Gate toolkit, a gift from Sylvestre Marillonnet (Addgene #1000000044; Engler et al. 2014). .. Briefly, targets were introduced into single guide RNA (sgRNA) scaffolds by PCR (for primers see Table S2) and assembled with a U6 small RNA promoter from either Arabidopsis (plCSL90002; Addgene #68261) or potato (Wang et al., 2015) into a Level 1 acceptor plasmid (plCH47751, plCH47761, plCH47772 or plCH47781).

    Clone Assay:

    Article Title: Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes.
    Article Snippet: .. Plasmid vectors for Cas9-mediated targeted mutagenesis were constructed according to Lawrenson et al. (2015) using the Plant Modular Cloning Golden Gate toolkit, a gift from Sylvestre Marillonnet (Addgene #1000000044; Engler et al. 2014). .. Briefly, targets were introduced into single guide RNA (sgRNA) scaffolds by PCR (for primers see Table S2) and assembled with a U6 small RNA promoter from either Arabidopsis (plCSL90002; Addgene #68261) or potato (Wang et al., 2015) into a Level 1 acceptor plasmid (plCH47751, plCH47761, plCH47772 or plCH47781).



    Similar Products

    94
    Cytiva Europe open source reconstruction toolkit
    Open Source Reconstruction Toolkit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/Tool+Kit/pmc13595413-67-10-20
    Average 94 stars, based on 1 article reviews
    open source reconstruction toolkit - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    99
    ATCC adipocyte differentiation tool kit
    Adipocyte Differentiation Tool Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/Adipocyte+Differentiation+Toolkit+for+Adipose-Derived+MSCs+and+Preadipocytes/pm41764532-52-15-19
    Average 99 stars, based on 1 article reviews
    adipocyte differentiation tool kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    94
    Cytiva Europe dosimetry toolkit
    Dosimetry Toolkit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/Tool+Kit/pmc13246971-318-9-11
    Average 94 stars, based on 1 article reviews
    dosimetry toolkit - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    99
    Qiagen dneasy powersoil tool kit
    Dneasy Powersoil Tool Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/DNeasy+PowerSoil+Pro+Kit/pm41775257-514-11-15
    Average 99 stars, based on 1 article reviews
    dneasy powersoil tool kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs nebuilder assembly tool
    Nebuilder Assembly Tool, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/NEBuilder+HiFi+DNA+Assembly+Cloning+Kit/pmc12886786-362-11-11
    Average 99 stars, based on 1 article reviews
    nebuilder assembly tool - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    86
    Ecogenomics Inc genome taxonomy database tool kit gtdb tk v2 4 1
    Genome Taxonomy Database Tool Kit Gtdb Tk V2 4 1, supplied by Ecogenomics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/database+genome+taxonomy/pm41634308-232-18-25
    Average 86 stars, based on 1 article reviews
    genome taxonomy database tool kit gtdb tk v2 4 1 - by Bioz Stars, 2026-11
    86/100 stars
      Buy from Supplier

    95
    Addgene inc moclo tool kit
    Moclo Tool Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/MoClo+Toolkit+(Kit+%231000000044)/pmc12868398-157-10-15
    Average 95 stars, based on 1 article reviews
    moclo tool kit - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    Image Search Results