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tm4sf4 cdnas  (OriGene)


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    OriGene tm4sf4 cdnas
    Figure 1: The C-termini of transmembrane 4 L six family members 1, 4, and 5 had similar effects on cell growth in 2D culture condition. A. Alignment of the C-terminal sequences of TM4SF1, <t>TM4SF4,</t> and TM4SF5 showed differences in the sequences around their cytosolic tails. B. Schemes of the wild-type (WT) and deletion or chimeric mutants used in the study. C. mRNA levels of tm4sf1, tm4sf4, and tm4sf5 in different colon cancer cells. D. Expression levels of the constructs (protein in left panel and mRNA in right panel) in stable HCT-116 cells. Within the TM4SF1 backbone (e.g., WT TM4SF1 [1WT], C-terminus-deleted TM4SF1 [1ΔC], and a chimera with the TM4SF5 C-terminus linked to the other parts of TM4SF1 [1C5]), the expression levels were similar each other. As for the TM4SF4 and TM4SF5 backbone, their expression levels were also comparable within each backbone. E. Growth of stable cells was monitored (from day 1 to day 2 after seeding) using an MTT assay, as described in the Materials and Methods. Values shown are the mean ± standard deviation (SD). *, **, and *** denote statistically significant differences at p <0.05, 0.005, or 0001, respectively, and ‘ns’ indicates a nonsignificant difference at p ≥ 0.05, by the ANOVA with Tukey’s post-tests. The data shown represent three independent experiments.
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    Images

    1) Product Images from "Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment."

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.

    Journal: Oncotarget

    doi: 10.18632/oncotarget.14809

    Figure 1: The C-termini of transmembrane 4 L six family members 1, 4, and 5 had similar effects on cell growth in 2D culture condition. A. Alignment of the C-terminal sequences of TM4SF1, TM4SF4, and TM4SF5 showed differences in the sequences around their cytosolic tails. B. Schemes of the wild-type (WT) and deletion or chimeric mutants used in the study. C. mRNA levels of tm4sf1, tm4sf4, and tm4sf5 in different colon cancer cells. D. Expression levels of the constructs (protein in left panel and mRNA in right panel) in stable HCT-116 cells. Within the TM4SF1 backbone (e.g., WT TM4SF1 [1WT], C-terminus-deleted TM4SF1 [1ΔC], and a chimera with the TM4SF5 C-terminus linked to the other parts of TM4SF1 [1C5]), the expression levels were similar each other. As for the TM4SF4 and TM4SF5 backbone, their expression levels were also comparable within each backbone. E. Growth of stable cells was monitored (from day 1 to day 2 after seeding) using an MTT assay, as described in the Materials and Methods. Values shown are the mean ± standard deviation (SD). *, **, and *** denote statistically significant differences at p <0.05, 0.005, or 0001, respectively, and ‘ns’ indicates a nonsignificant difference at p ≥ 0.05, by the ANOVA with Tukey’s post-tests. The data shown represent three independent experiments.
    Figure Legend Snippet: Figure 1: The C-termini of transmembrane 4 L six family members 1, 4, and 5 had similar effects on cell growth in 2D culture condition. A. Alignment of the C-terminal sequences of TM4SF1, TM4SF4, and TM4SF5 showed differences in the sequences around their cytosolic tails. B. Schemes of the wild-type (WT) and deletion or chimeric mutants used in the study. C. mRNA levels of tm4sf1, tm4sf4, and tm4sf5 in different colon cancer cells. D. Expression levels of the constructs (protein in left panel and mRNA in right panel) in stable HCT-116 cells. Within the TM4SF1 backbone (e.g., WT TM4SF1 [1WT], C-terminus-deleted TM4SF1 [1ΔC], and a chimera with the TM4SF5 C-terminus linked to the other parts of TM4SF1 [1C5]), the expression levels were similar each other. As for the TM4SF4 and TM4SF5 backbone, their expression levels were also comparable within each backbone. E. Growth of stable cells was monitored (from day 1 to day 2 after seeding) using an MTT assay, as described in the Materials and Methods. Values shown are the mean ± standard deviation (SD). *, **, and *** denote statistically significant differences at p <0.05, 0.005, or 0001, respectively, and ‘ns’ indicates a nonsignificant difference at p ≥ 0.05, by the ANOVA with Tukey’s post-tests. The data shown represent three independent experiments.

    Techniques Used: Expressing, Construct, MTT Assay, Standard Deviation

    Figure 3: TM4SF1 and TM4SF5 but not TM4SF4 promoted enhanced transwell migration, and the TM4SF5 C-terminus could replace the pro-migratory effect of the TM4SF1 C-terminus. A. Cells incubated with 1% BSA-DMEM for 2 h were loaded (105 cells/condition) to the transwell chambers, the bottom sides of which were pre-coated with collagen I (10 μg/ml). After 10 h, cells that had migrated through the transwell chambers were stained with 5% crystal violet, and images were then acquired randomly; representative images are shown. B. The number of migrated cells from 10 random images per experimental condition were determined using an Image J software based on the intensity of staining. Values shown are the mean ± SD. Immunoblots for phospho-Tyr416 in c-Src and c-Src were performed using whole-cell lysates of the stable cells. *, **, and *** denote statistically significant differences at p <0.05, 0.005, or 0001, respectively, and ‘ns’ indicates a nonsignificant difference at p ≥ 0.05 by the ANOVA with Tukey’s post-tests. The data shown represent three independent experiments.
    Figure Legend Snippet: Figure 3: TM4SF1 and TM4SF5 but not TM4SF4 promoted enhanced transwell migration, and the TM4SF5 C-terminus could replace the pro-migratory effect of the TM4SF1 C-terminus. A. Cells incubated with 1% BSA-DMEM for 2 h were loaded (105 cells/condition) to the transwell chambers, the bottom sides of which were pre-coated with collagen I (10 μg/ml). After 10 h, cells that had migrated through the transwell chambers were stained with 5% crystal violet, and images were then acquired randomly; representative images are shown. B. The number of migrated cells from 10 random images per experimental condition were determined using an Image J software based on the intensity of staining. Values shown are the mean ± SD. Immunoblots for phospho-Tyr416 in c-Src and c-Src were performed using whole-cell lysates of the stable cells. *, **, and *** denote statistically significant differences at p <0.05, 0.005, or 0001, respectively, and ‘ns’ indicates a nonsignificant difference at p ≥ 0.05 by the ANOVA with Tukey’s post-tests. The data shown represent three independent experiments.

    Techniques Used: Migration, Incubation, Staining, Software, Western Blot

    Figure 5: TM4SF5 and its C-terminus could positively replace the effects of TM4SF1 and TM4SF4 to promote invasive dissemination from spheroids embedded in 3D collagen I gels. A. Scheme of the ‘on-top’ 3D ECM-surrounded culture environment, in which the cells (red) were mixed with a lower concentration (yellow-pink) of collagen I gel (0.2 mg/ml) and overlaid on the bottom dense layer with collagen I gel at a higher concentration (blue, 2 mg/ml) that was previously solidified. B to E. Representative images of the starting (each left) and ending (each right) points of the live-imaged spheroids in each condition were shown (B and E). The two-tailed unpaired Student’s t-test was used for the analysis in (B). * denotes statistically a significant difference at p <0.05, but ‘ns’ insignificantly at p ≥0.05. The dissemination initiation times (C) or numbers of cells disseminating (D) from the spheroids were measured during the live- imaging for 36 (C) or 24 (D) h and are presented in the graph as mean ± SD values. White arrow heads depict the disseminating cells (B). Immunoblotting for phospho-Tyr397 in FAK and FAK was performed by using the whole cell lysates prepared from the spheroids in 3D collagen I gels. PP2 or PP3 (10 μM) was added to the gels during the embedding processes, before 24 h live-imaging (E).
    Figure Legend Snippet: Figure 5: TM4SF5 and its C-terminus could positively replace the effects of TM4SF1 and TM4SF4 to promote invasive dissemination from spheroids embedded in 3D collagen I gels. A. Scheme of the ‘on-top’ 3D ECM-surrounded culture environment, in which the cells (red) were mixed with a lower concentration (yellow-pink) of collagen I gel (0.2 mg/ml) and overlaid on the bottom dense layer with collagen I gel at a higher concentration (blue, 2 mg/ml) that was previously solidified. B to E. Representative images of the starting (each left) and ending (each right) points of the live-imaged spheroids in each condition were shown (B and E). The two-tailed unpaired Student’s t-test was used for the analysis in (B). * denotes statistically a significant difference at p <0.05, but ‘ns’ insignificantly at p ≥0.05. The dissemination initiation times (C) or numbers of cells disseminating (D) from the spheroids were measured during the live- imaging for 36 (C) or 24 (D) h and are presented in the graph as mean ± SD values. White arrow heads depict the disseminating cells (B). Immunoblotting for phospho-Tyr397 in FAK and FAK was performed by using the whole cell lysates prepared from the spheroids in 3D collagen I gels. PP2 or PP3 (10 μM) was added to the gels during the embedding processes, before 24 h live-imaging (E).

    Techniques Used: Concentration Assay, Two Tailed Test, Imaging, Western Blot

    Related Articles

    Expressing:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Construct:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    MTT Assay:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Standard Deviation:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Migration:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Incubation:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Staining:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Software:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Western Blot:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Concentration Assay:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Two Tailed Test:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.

    Imaging:

    Article Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
    Article Snippet: cDNAs Preparation of TM4SF5 cDNA was described previously [24].cDNAs Preparation of TM4SF5 cDNA was described previously [24].. TM4SF1 and TM4SF4 cDNAs were purchased from Origene (Rockville, MD, USA).. Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.Mutant DNA constructs, in which the C-terminus of TM4SF1, TM4SF4, and TM4SF5 was deleted or exchanged for one of the others, were cloned from the WT cDNAs using PCR.



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    86
    Fisher Scientific trimethoprim tmp
    ( A ) 4 dpci <t>DMSO-treated</t> <t>and</t> <t>TGF-β</t> inhibitor-treated ventricular sections stained for MyHC (CMs, green), mCherry (macrophages, magenta), and DAPI (DNA, blue). Arrowheads point to mpeg1.1 :mCherryF + cells. ( B ) Number of mpeg1.1 :mCherryF + cells per mm 2 injured area in DMSO-treated ( n = 6) and TGFβ inhibitor-treated ( n = 5) ventricles at 4 dpci. ( C ) RT-qPCR analysis of tnfa, il6 and il1b mRNA levels in control (Ctrl) ( n = 4) and TGF-β inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( D ) RT-qPCR analysis of ccl20a.3 and cdaa mRNA levels in control (Ctrl) ( n = 5) and TGFβ inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( E ) 4 dpci sulf1 +/+ , sulf1 +/+ dexamethasone-treated (DEX), sulf1 −/− and sulf1 −/− dexamethasone-treated ventricular sections stained for pSmad3 (magenta) and DAPI (DNA, blue). Arrowheads point to pSmad3 + cells. ( F ) Number of pSmad3 + cells per mm 2 injured area in sulf1 +/+ ( n = 4), sulf1 +/+ dexamethasone-treated ( n = 4), sulf1 −/− ( n = 5) and sulf1 −/− dexamethasone-treated ( n = 6) ventricles at 4 dpci. ( G ) 30 dpci sulf1 −/− DMSO-treated and sulf1 −/− dexamethasone-treated ventricular sections stained with AFOG for muscle (yellow), collagen (blue), and fibrin (red). ( H ) Percentage representation of groups of different fibrotic area sizes at 30 dpci for cryoinjured sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles. Black dotted lines delineate the area with collagen and fibrin deposits. ( I ) Percentage of collagen/fibrin in fibrotic area in sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles at 30 dpci. Scale bars: 100 µm. White dashed lines delineate the injured area. Statistical tests: Nonparametric Mann–Whitney test ( C , D ), Student’s t test ( B , F , I ). For the boxplots, the center line indicates the median; the box bounds represent the 25th and 75th percentiles; the whiskers extend to the most extreme data points; and each point represents an independent measurement. .
    Trimethoprim Tmp, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmp/trimethoprim/10__1002_slash_cmtd__202500165-136-0-5
    Average 86 stars, based on 1 article reviews
    trimethoprim tmp - by Bioz Stars, 2026-10
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    transmembrane pressure tmp  (Sterlitech corporation)
    91
    Sterlitech corporation transmembrane pressure tmp
    ( A ) 4 dpci <t>DMSO-treated</t> <t>and</t> <t>TGF-β</t> inhibitor-treated ventricular sections stained for MyHC (CMs, green), mCherry (macrophages, magenta), and DAPI (DNA, blue). Arrowheads point to mpeg1.1 :mCherryF + cells. ( B ) Number of mpeg1.1 :mCherryF + cells per mm 2 injured area in DMSO-treated ( n = 6) and TGFβ inhibitor-treated ( n = 5) ventricles at 4 dpci. ( C ) RT-qPCR analysis of tnfa, il6 and il1b mRNA levels in control (Ctrl) ( n = 4) and TGF-β inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( D ) RT-qPCR analysis of ccl20a.3 and cdaa mRNA levels in control (Ctrl) ( n = 5) and TGFβ inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( E ) 4 dpci sulf1 +/+ , sulf1 +/+ dexamethasone-treated (DEX), sulf1 −/− and sulf1 −/− dexamethasone-treated ventricular sections stained for pSmad3 (magenta) and DAPI (DNA, blue). Arrowheads point to pSmad3 + cells. ( F ) Number of pSmad3 + cells per mm 2 injured area in sulf1 +/+ ( n = 4), sulf1 +/+ dexamethasone-treated ( n = 4), sulf1 −/− ( n = 5) and sulf1 −/− dexamethasone-treated ( n = 6) ventricles at 4 dpci. ( G ) 30 dpci sulf1 −/− DMSO-treated and sulf1 −/− dexamethasone-treated ventricular sections stained with AFOG for muscle (yellow), collagen (blue), and fibrin (red). ( H ) Percentage representation of groups of different fibrotic area sizes at 30 dpci for cryoinjured sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles. Black dotted lines delineate the area with collagen and fibrin deposits. ( I ) Percentage of collagen/fibrin in fibrotic area in sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles at 30 dpci. Scale bars: 100 µm. White dashed lines delineate the injured area. Statistical tests: Nonparametric Mann–Whitney test ( C , D ), Student’s t test ( B , F , I ). For the boxplots, the center line indicates the median; the box bounds represent the 25th and 75th percentiles; the whiskers extend to the most extreme data points; and each point represents an independent measurement. .
    Transmembrane Pressure Tmp, supplied by Sterlitech corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmp/TAMI+Ceramic+Membrane+Complete+Disc-Holder/10__1016_slash_j__ceja__2026__101171-109-16-12
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    trimethylphosphate tmp  (MedChemExpress)
    94
    MedChemExpress trimethylphosphate tmp
    ( A ) 4 dpci <t>DMSO-treated</t> <t>and</t> <t>TGF-β</t> inhibitor-treated ventricular sections stained for MyHC (CMs, green), mCherry (macrophages, magenta), and DAPI (DNA, blue). Arrowheads point to mpeg1.1 :mCherryF + cells. ( B ) Number of mpeg1.1 :mCherryF + cells per mm 2 injured area in DMSO-treated ( n = 6) and TGFβ inhibitor-treated ( n = 5) ventricles at 4 dpci. ( C ) RT-qPCR analysis of tnfa, il6 and il1b mRNA levels in control (Ctrl) ( n = 4) and TGF-β inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( D ) RT-qPCR analysis of ccl20a.3 and cdaa mRNA levels in control (Ctrl) ( n = 5) and TGFβ inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( E ) 4 dpci sulf1 +/+ , sulf1 +/+ dexamethasone-treated (DEX), sulf1 −/− and sulf1 −/− dexamethasone-treated ventricular sections stained for pSmad3 (magenta) and DAPI (DNA, blue). Arrowheads point to pSmad3 + cells. ( F ) Number of pSmad3 + cells per mm 2 injured area in sulf1 +/+ ( n = 4), sulf1 +/+ dexamethasone-treated ( n = 4), sulf1 −/− ( n = 5) and sulf1 −/− dexamethasone-treated ( n = 6) ventricles at 4 dpci. ( G ) 30 dpci sulf1 −/− DMSO-treated and sulf1 −/− dexamethasone-treated ventricular sections stained with AFOG for muscle (yellow), collagen (blue), and fibrin (red). ( H ) Percentage representation of groups of different fibrotic area sizes at 30 dpci for cryoinjured sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles. Black dotted lines delineate the area with collagen and fibrin deposits. ( I ) Percentage of collagen/fibrin in fibrotic area in sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles at 30 dpci. Scale bars: 100 µm. White dashed lines delineate the injured area. Statistical tests: Nonparametric Mann–Whitney test ( C , D ), Student’s t test ( B , F , I ). For the boxplots, the center line indicates the median; the box bounds represent the 25th and 75th percentiles; the whiskers extend to the most extreme data points; and each point represents an independent measurement. .
    Trimethylphosphate Tmp, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmp/Trimethyl+phosphate/10__1016_slash_j__colsurfa__2026__140609-192-22-43
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    tmp  (Tokyo Chemical Industry)
    86
    Tokyo Chemical Industry tmp
    ( A ) 4 dpci <t>DMSO-treated</t> <t>and</t> <t>TGF-β</t> inhibitor-treated ventricular sections stained for MyHC (CMs, green), mCherry (macrophages, magenta), and DAPI (DNA, blue). Arrowheads point to mpeg1.1 :mCherryF + cells. ( B ) Number of mpeg1.1 :mCherryF + cells per mm 2 injured area in DMSO-treated ( n = 6) and TGFβ inhibitor-treated ( n = 5) ventricles at 4 dpci. ( C ) RT-qPCR analysis of tnfa, il6 and il1b mRNA levels in control (Ctrl) ( n = 4) and TGF-β inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( D ) RT-qPCR analysis of ccl20a.3 and cdaa mRNA levels in control (Ctrl) ( n = 5) and TGFβ inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( E ) 4 dpci sulf1 +/+ , sulf1 +/+ dexamethasone-treated (DEX), sulf1 −/− and sulf1 −/− dexamethasone-treated ventricular sections stained for pSmad3 (magenta) and DAPI (DNA, blue). Arrowheads point to pSmad3 + cells. ( F ) Number of pSmad3 + cells per mm 2 injured area in sulf1 +/+ ( n = 4), sulf1 +/+ dexamethasone-treated ( n = 4), sulf1 −/− ( n = 5) and sulf1 −/− dexamethasone-treated ( n = 6) ventricles at 4 dpci. ( G ) 30 dpci sulf1 −/− DMSO-treated and sulf1 −/− dexamethasone-treated ventricular sections stained with AFOG for muscle (yellow), collagen (blue), and fibrin (red). ( H ) Percentage representation of groups of different fibrotic area sizes at 30 dpci for cryoinjured sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles. Black dotted lines delineate the area with collagen and fibrin deposits. ( I ) Percentage of collagen/fibrin in fibrotic area in sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles at 30 dpci. Scale bars: 100 µm. White dashed lines delineate the injured area. Statistical tests: Nonparametric Mann–Whitney test ( C , D ), Student’s t test ( B , F , I ). For the boxplots, the center line indicates the median; the box bounds represent the 25th and 75th percentiles; the whiskers extend to the most extreme data points; and each point represents an independent measurement. .
    Tmp, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmp/tmp/pm41892445-178-0-7
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    o procedimento indicado foi tmp com o sistema clottriever  (Inari Medical Inc)
    86
    Inari Medical Inc o procedimento indicado foi tmp com o sistema clottriever
    Radioscopia demonstra o <t>procedimento</t> de remoção de trombo da veia femoral profunda esquerda. (A) Inserido pela veia femoral comum direita, o introdutor longo 6Fr x 55cm foi posicionado na origem da veia femoral profunda esquerda. Pelo introdutor longo, o cateter Fogarty n° 4 foi inserido, posicionado no segmento distal da veia femoral comum esquerda e insuflado com contraste diluído em solução fisiológica; (B) O <t>sistema</t> <t>ClotTriever®</t> foi posicionado aberto no segmento ilíaco esquerdo e o conjunto introdutor-Fogarty foi tracionado até a veia femoral comum.
    O Procedimento Indicado Foi Tmp Com O Sistema Clottriever, supplied by Inari Medical Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) 4 dpci DMSO-treated and TGF-β inhibitor-treated ventricular sections stained for MyHC (CMs, green), mCherry (macrophages, magenta), and DAPI (DNA, blue). Arrowheads point to mpeg1.1 :mCherryF + cells. ( B ) Number of mpeg1.1 :mCherryF + cells per mm 2 injured area in DMSO-treated ( n = 6) and TGFβ inhibitor-treated ( n = 5) ventricles at 4 dpci. ( C ) RT-qPCR analysis of tnfa, il6 and il1b mRNA levels in control (Ctrl) ( n = 4) and TGF-β inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( D ) RT-qPCR analysis of ccl20a.3 and cdaa mRNA levels in control (Ctrl) ( n = 5) and TGFβ inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( E ) 4 dpci sulf1 +/+ , sulf1 +/+ dexamethasone-treated (DEX), sulf1 −/− and sulf1 −/− dexamethasone-treated ventricular sections stained for pSmad3 (magenta) and DAPI (DNA, blue). Arrowheads point to pSmad3 + cells. ( F ) Number of pSmad3 + cells per mm 2 injured area in sulf1 +/+ ( n = 4), sulf1 +/+ dexamethasone-treated ( n = 4), sulf1 −/− ( n = 5) and sulf1 −/− dexamethasone-treated ( n = 6) ventricles at 4 dpci. ( G ) 30 dpci sulf1 −/− DMSO-treated and sulf1 −/− dexamethasone-treated ventricular sections stained with AFOG for muscle (yellow), collagen (blue), and fibrin (red). ( H ) Percentage representation of groups of different fibrotic area sizes at 30 dpci for cryoinjured sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles. Black dotted lines delineate the area with collagen and fibrin deposits. ( I ) Percentage of collagen/fibrin in fibrotic area in sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles at 30 dpci. Scale bars: 100 µm. White dashed lines delineate the injured area. Statistical tests: Nonparametric Mann–Whitney test ( C , D ), Student’s t test ( B , F , I ). For the boxplots, the center line indicates the median; the box bounds represent the 25th and 75th percentiles; the whiskers extend to the most extreme data points; and each point represents an independent measurement. .

    Journal: EMBO Reports

    Article Title: Sulf1 regulates inflammation via modulation of TGF-β dependent epicardial activation

    doi: 10.1038/s44319-026-00910-5

    Figure Lengend Snippet: ( A ) 4 dpci DMSO-treated and TGF-β inhibitor-treated ventricular sections stained for MyHC (CMs, green), mCherry (macrophages, magenta), and DAPI (DNA, blue). Arrowheads point to mpeg1.1 :mCherryF + cells. ( B ) Number of mpeg1.1 :mCherryF + cells per mm 2 injured area in DMSO-treated ( n = 6) and TGFβ inhibitor-treated ( n = 5) ventricles at 4 dpci. ( C ) RT-qPCR analysis of tnfa, il6 and il1b mRNA levels in control (Ctrl) ( n = 4) and TGF-β inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( D ) RT-qPCR analysis of ccl20a.3 and cdaa mRNA levels in control (Ctrl) ( n = 5) and TGFβ inhibitor-treated ( n = 5) cryoinjured ventricles at 4 dpci. ( E ) 4 dpci sulf1 +/+ , sulf1 +/+ dexamethasone-treated (DEX), sulf1 −/− and sulf1 −/− dexamethasone-treated ventricular sections stained for pSmad3 (magenta) and DAPI (DNA, blue). Arrowheads point to pSmad3 + cells. ( F ) Number of pSmad3 + cells per mm 2 injured area in sulf1 +/+ ( n = 4), sulf1 +/+ dexamethasone-treated ( n = 4), sulf1 −/− ( n = 5) and sulf1 −/− dexamethasone-treated ( n = 6) ventricles at 4 dpci. ( G ) 30 dpci sulf1 −/− DMSO-treated and sulf1 −/− dexamethasone-treated ventricular sections stained with AFOG for muscle (yellow), collagen (blue), and fibrin (red). ( H ) Percentage representation of groups of different fibrotic area sizes at 30 dpci for cryoinjured sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles. Black dotted lines delineate the area with collagen and fibrin deposits. ( I ) Percentage of collagen/fibrin in fibrotic area in sulf1 −/− DMSO-treated ( n = 6) and sulf1 −/− dexamethasone-treated ( n = 5) ventricles at 30 dpci. Scale bars: 100 µm. White dashed lines delineate the injured area. Statistical tests: Nonparametric Mann–Whitney test ( C , D ), Student’s t test ( B , F , I ). For the boxplots, the center line indicates the median; the box bounds represent the 25th and 75th percentiles; the whiskers extend to the most extreme data points; and each point represents an independent measurement. .

    Article Snippet: For the TGF-β inhibitor experiment, fish were incubated in system water supplemented with TGF-β inhibitor (Cat. no. SB431542, 50 mg BOC Sciences) or DMSO at a concentration of 10 μM per fish starting 1 day prior to cryoinjuries until 4 dpci when the hearts were extracted.

    Techniques: Staining, Quantitative RT-PCR, Analysis, Control, MANN-WHITNEY

    Radioscopia demonstra o procedimento de remoção de trombo da veia femoral profunda esquerda. (A) Inserido pela veia femoral comum direita, o introdutor longo 6Fr x 55cm foi posicionado na origem da veia femoral profunda esquerda. Pelo introdutor longo, o cateter Fogarty n° 4 foi inserido, posicionado no segmento distal da veia femoral comum esquerda e insuflado com contraste diluído em solução fisiológica; (B) O sistema ClotTriever® foi posicionado aberto no segmento ilíaco esquerdo e o conjunto introdutor-Fogarty foi tracionado até a veia femoral comum.

    Journal: Jornal Vascular Brasileiro

    Article Title: Percutaneous mechanical thrombectomy in a patient with extensive deep vein thrombosis: pioneering surgery in the northern region

    doi: 10.1590/1677-5449.202501162

    Figure Lengend Snippet: Radioscopia demonstra o procedimento de remoção de trombo da veia femoral profunda esquerda. (A) Inserido pela veia femoral comum direita, o introdutor longo 6Fr x 55cm foi posicionado na origem da veia femoral profunda esquerda. Pelo introdutor longo, o cateter Fogarty n° 4 foi inserido, posicionado no segmento distal da veia femoral comum esquerda e insuflado com contraste diluído em solução fisiológica; (B) O sistema ClotTriever® foi posicionado aberto no segmento ilíaco esquerdo e o conjunto introdutor-Fogarty foi tracionado até a veia femoral comum.

    Article Snippet: O procedimento indicado foi TMP com o sistema ClotTriever® (Inari Medical, Inc., EUA), abrangendo os segmentos cavo-ilíaco e fêmoro-poplíteo do MIE.

    Techniques: