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tianscript rt kit  (tiangen biotech co)


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  • 99

    Structured Review

    tiangen biotech co tianscript rt kit
    Tianscript Rt Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tianscript+rt+kit/TIANScript+RT+Kit/pm41844782-89-14-17
    Average 99 stars, based on 189 article reviews
    tianscript rt kit - by Bioz Stars, 2026-10
    99/100 stars

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    Reverse Transcription:

    Article Title: CircFoxo3 knockdown inhibit gastric cancer progression by regulating Foxo3.
    Article Snippet: Improving the diagnosis and treatment of gastric cancer is a significant challenge worldwide.. Circular RNAs (circRNAs), a recently identified class of endogenous non-coding RNAs with covalently closed-loop structures, have emerged as key regulators in tumorigenesis.. CircFoxo3 has been studied in various cancer types, while its functional role in GC remains poorly understood.

    Article Title: High COMP expression promotes tumor cell proliferation and migration of rectal cancer, contributing to unfavorable prognosis and suppressive immune microenvironment.
    Article Snippet: 2.9 RNA extraction and quantitative-PCR (qPCR) Total RNA was isolated using Trizol reagent (Cat# 15596-026, Invitrogen, Grand Island, CA, USA) and quantified by Nanodrop lite (Thermo, USA). .. Reverse transcription was conducted to obtain complementary DNA (cDNA) employing TIANScript RT kit (Cat# KR104-02, TIANGEN, Beijing, China). .. The quantitative PCR experiment was carried out and repeated three times by Light-Cycler480 (Roche, USA) for 40 cycles.

    Article Title: Benzalkonium chloride adaptation induces filamentation in Listeria monocytogenes HL06 and enhances its growth in pasteurized milk and pork sausage at 4°C
    Article Snippet: Benzalkonium chloride (BC) is a commonly used disinfectant in the food industry.. However, inappropriate use of BC leads to BC adaptation in Listeria monocytogenes.. BC adaptation can induce L. monocytogenes filamentation; however, the underlying mechanism remains unclear.

    Quantitative RT-PCR:

    Article Title: CircFoxo3 knockdown inhibit gastric cancer progression by regulating Foxo3.
    Article Snippet: Improving the diagnosis and treatment of gastric cancer is a significant challenge worldwide.. Circular RNAs (circRNAs), a recently identified class of endogenous non-coding RNAs with covalently closed-loop structures, have emerged as key regulators in tumorigenesis.. CircFoxo3 has been studied in various cancer types, while its functional role in GC remains poorly understood.

    Synthesized:

    Article Title: Proteomic analysis reveals selenium-induced metabolic alterations in forest-grown ginseng.
    Article Snippet: .. The first strand of cDNA for real-time q-PCR analysis was synthesized by using a TIANScript RT kit (Tiangen). .. Q-PCR analysis was performed to validate the gene expression of DEPs by using an iQ5 real-time PCR system (Bio-Rad, USA).

    Article Title: Loss of the plastidial hexokinase CsHXK3 triggers metabolic starvation and ROS imbalance leading to pollen abortion in cucumber.
    Article Snippet: Lijun Lv, Lidong Zhang, Hujian Li , Zhen Yang, Yicong Guo, Yuzi Shi, Qian Zhang, Hongyu Huang, Yuhe Li, Xuehui Yao, Xiaoyue Zhu, Lingjun Xu, Jing Nie and Xiaolei Sui Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, China, State Key Laboratory of Vegetable Biobreeding, Tianjin Academy of Agricultural Sciences, Tianjin 300192, China, and Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Longping Agricultural College, Hunan University, Changsha 410082, China

    Isolation:

    Article Title: Polyphenol-rich Chinese olive extracts attenuate lipid accumulation in HepG2 cells, accompanied by AMPK phosphorylation and miRNA alterations
    Article Snippet: Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM) containing 4,500 mg/L glucose, penicillin G (100 units/mL) and streptomycin (100 μg/mL) were purchased from Thermo Fisher Scientific Inc. (Massachusetts, United States). .. TRNzol Universal Reagent, the miRNAcute miRNA Isolation Kit, FastKing gDNA Dispelling RT SuperMix, the miRNA 1st Strand cDNA Synthesis Kit, TIANScript II RT Kit and RNase-free ddH 2 O were purchased from TIANGEN Biotech Co., Ltd. (Beijing, China). .. SYBR Green Master Mix was procured from Nanjing Vazyme Biotech Co., Ltd. (Nanjing, China).

    cDNA Synthesis:

    Article Title: Polyphenol-rich Chinese olive extracts attenuate lipid accumulation in HepG2 cells, accompanied by AMPK phosphorylation and miRNA alterations
    Article Snippet: Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM) containing 4,500 mg/L glucose, penicillin G (100 units/mL) and streptomycin (100 μg/mL) were purchased from Thermo Fisher Scientific Inc. (Massachusetts, United States). .. TRNzol Universal Reagent, the miRNAcute miRNA Isolation Kit, FastKing gDNA Dispelling RT SuperMix, the miRNA 1st Strand cDNA Synthesis Kit, TIANScript II RT Kit and RNase-free ddH 2 O were purchased from TIANGEN Biotech Co., Ltd. (Beijing, China). .. SYBR Green Master Mix was procured from Nanjing Vazyme Biotech Co., Ltd. (Nanjing, China).



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