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tfr coding sequence  (Addgene inc)


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    Structured Review

    Addgene inc tfr coding sequence
    Tfr Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tfr+coding+sequence/TfR-sfGFP-myc+tag-SpyCatcher003+(Plasmid+%23133451)/pm37791900-260-1-7
    Average 91 stars, based on 7 article reviews
    tfr coding sequence - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Sequencing:

    Article Title: Zero-Mode Waveguide Nanowells for Single-Molecule Detection in Living Cells.
    Article Snippet: .. The TfR coding sequence was amplified from Addgene plasmid #133451. ..

    Article Title: Zero-Mode Waveguide Nanowells for Single-Molecule Detection in Living Cells
    Article Snippet: .. The TfR coding sequence was amplified from Addgene plasmid #133451. ..

    Amplification:

    Article Title: Zero-Mode Waveguide Nanowells for Single-Molecule Detection in Living Cells.
    Article Snippet: .. The TfR coding sequence was amplified from Addgene plasmid #133451. ..

    Article Title: Zero-Mode Waveguide Nanowells for Single-Molecule Detection in Living Cells
    Article Snippet: .. The TfR coding sequence was amplified from Addgene plasmid #133451. ..

    Plasmid Preparation:

    Article Title: Zero-Mode Waveguide Nanowells for Single-Molecule Detection in Living Cells.
    Article Snippet: .. The TfR coding sequence was amplified from Addgene plasmid #133451. ..

    Article Title: Zero-Mode Waveguide Nanowells for Single-Molecule Detection in Living Cells
    Article Snippet: .. The TfR coding sequence was amplified from Addgene plasmid #133451. ..

    Isolation:

    Article Title: A modular approach to enhancing cell membrane-coated nanoparticle functionality using genetic engineering.
    Article Snippet: Since their initial development, cell membrane-coated nanoparticles (CNPs) have become increasingly popular in the biomedical field.. Despite their inherent versatility and ability to enable complex biological applications, there is considerable interest in augmenting the performance of CNPs through the introduction of additional functionalities.. Here we demonstrate a genetic-engineering-based modular approach to CNP functionalization that can encompass a wide range of ligands onto the nanoparticle surface.

    Polymerase Chain Reaction:

    Article Title: A modular approach to enhancing cell membrane-coated nanoparticle functionality using genetic engineering.
    Article Snippet: Since their initial development, cell membrane-coated nanoparticles (CNPs) have become increasingly popular in the biomedical field.. Despite their inherent versatility and ability to enable complex biological applications, there is considerable interest in augmenting the performance of CNPs through the introduction of additional functionalities.. Here we demonstrate a genetic-engineering-based modular approach to CNP functionalization that can encompass a wide range of ligands onto the nanoparticle surface.

    Derivative Assay:

    Article Title: Visible Light-Induced Specific Protein Reaction Delineates Early Stages of Cell Adhesion
    Article Snippet: .. The amber stop codon TAG instead of the lysine at position 31 of SpyCatcher003 allows for the incorporation of 7-hydroxycoumarin-caged lysine (HCK) when co-expressed with HCK tRNA synthetase (HCK RS) and PylT. pcDNA3-TfR-SpyCatcher003-superfolder green fluorescent protein (sfGFP) and pcDNA3-TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag (GenBank OR711470, Addgene ID 210021) with 1× U6-PylT and 1× H1-PylT were derived from pcDNA3-mCherryTAG-EGFP and pENTR4-TfR-sfGFP-SpyCatcher003 (GenBank MN433890, Addgene ID 133451),47 which have been described. .. TfR comprises the Transferrin receptor transmembrane domain and cytosolic region with Y20C and F23A mutations to block internalization and allows for mammalian cell surface expression of SpyCatcher003.47 In this context, sfGFP should only be expressed when HCK has successfully been incorporated in the growing polypeptide chain. pcDNA3-LifeAct-mNeonGreen-IRES-Talin head-SpyTag003 (GenBank OR711471, Addgene ID 210024) with 1× U6-PylT and 1× H1-PylT was derived from pcDNA3-EGFPTalin head-SpyCatcher003 and encodes mouse codon-optimized LifeAct-mNeonGreen, followed by encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) and Talin head-SpyTag003. pcDNA3-Talin head-SpyTag003 was derived from pcDNA3-LifeActmNeonGreen-IRES-Talin head-SpyTag003 by removing the cassette encoding LifeActmNeonGreen and IRES. pEGFP-C1 SpyCatcher003(K31TAG)-Talin rod (434-2541)- mScarletI (GenBank OR711472, Addgene ID 210022), pEGFP-C1 SpyTag003(V114T, V116T)-Talin rod (434-2541)-mCherry (Addgene ID 210027), pEGFP-C1 SpyTag003(M115A)-Talin rod (434-2541)-mCherry (Addgene ID 210025) and pEGFP-C1 SpyTag003(M115G)-Talin rod (434-2541)-mCherry (Addgene ID 210026) were derived from pEGFP-C1 SpyTag003-Talin rod (434-2541)-mCherry (GenBank MN527524 and Addgene ID 133567) previously described.47 In the context of pEGFP-C1 SpyCatcher003(K31TAG)-Talin rod (434-2541)-mScarletI, mScarletI should only be expressed when HCK has successfully been incorporated in the growing polypeptide chain.



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    91
    Addgene inc tfr coding sequence
    Tfr Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tfr+coding+sequence/TfR-sfGFP-myc+tag-SpyCatcher003+(Plasmid+%23133451)/pm37791900-260-1-7
    Average 91 stars, based on 1 article reviews
    tfr coding sequence - by Bioz Stars, 2026-09
    91/100 stars
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