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3 3 5 5 tetramethylbenzidine substrate  (Elabscience Biotechnology)


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    Elabscience Biotechnology 3 3 5 5 tetramethylbenzidine substrate
    3 3 5 5 Tetramethylbenzidine Substrate, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tetramethylbenzidine+substrate/Excellent+Chemiluminescent+Substrate+(ECL)Detection+Kit/pmc13107097-112-0-4
    Average 96 stars, based on 125 article reviews
    3 3 5 5 tetramethylbenzidine substrate - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Morpho-Functional Effects of Nonylphenol-Steroid Hormone Co-Exposure on Human Prostate PNT1A Cells.
    Article Snippet: Protein molecular weights were estimated using the Opti-Protein Ultra Marker (cat. no. G623; Applied Biological Materials Inc., Richmond, BC, Canada), a pre-stained protein ladder covering a range from 6.5 to 270 kDa. .. After incubation, protein bands were detected using a chemiluminescent HRP substrate (Cat. No. E-IR-R307, Elabscience Bionovation Inc., Houston, TX, USA) and visualized with a chemiluminescence scanner (ChemiDoc Imaging System, Bio-Rad Laboratories, Hercules, CA, USA). .. Band intensities were quantified using ImageJ 1.54f software (NIH, Bethesda, MD, USA).

    Article Title: MIIP Inhibits Colorectal Cancer Progression by Modulating Neutrophils Infiltration and Neutrophil Extracellular Trap Formation
    Article Snippet: The primary antibodies used were as follows: anti‐β‐Actin (1:1000, 4967, CST, USA; RRID: AB_330288), anti‐LaminB1 (1:1000, 12586, CST, USA; RRID: AB_2650517), anti‐MIIP (1:1000, HPA044948, Sigma, USA; RRID: AB_10795887), anti‐MIIP (1:1000, orb537083, Biobyrt, USA; RRID: AB_3665851), anti‐PKR (1:200, SC‐6282, Santa Cruz; RRID: AB_628150), anti‐NF‐κB p65 (1:1000, 8242, CST, USA; RRID: AB_10859369), and anti‐phospho‐NF‐κB p65 (Ser276) (1:1000, AF3387, Affinity, China). .. Afterward, the membranes were incubated with anti‐rabbit/mouse IgG secondary antibodies (1:5000, 7074 or 7076; CST, USA; RRID: AB_2099233 or RRID: AB_330924) at room temperature for 1 h. Enhanced chemiluminescence reagent (E‐IR‐R307; Elabscience, China) was used for signal detection after secondary antibody incubation. .. Total RNA was isolated from the cells using TRIzol (15596026; Invitrogen, USA) and chloroform (CX1060; Merck, Germany).

    Article Title: MIIP Inhibits Colorectal Cancer Progression by Modulating Neutrophils Infiltration and Neutrophil Extracellular Trap Formation.
    Article Snippet: The primary antibodies used were as follows: anti- β-Actin (1:1000, 4967, CST, USA; RRID: AB_330288), anti-LaminB1 (1:1000, 12586, CST, USA; RRID: AB_2650517), antiMIIP (1:1000, HPA044948, Sigma, USA; RRID: AB_10795887), anti-MIIP (1:1000, orb537083, Biobyrt, USA; RRID: AB_3665851), anti-PKR (1:200, SC-6282, Santa Cruz; RRID: AB_628150), antiNF- κB p65 (1:1000, 8242, CST, USA; RRID: AB_10859369), and anti-phospho-NF- κB p65 (Ser276) (1:1000, AF3387, Affinity, China). .. Afterward, the membranes were incubated with antirabbit/mouse IgG secondary antibodies (1:5000, 7074 or 7076; CST, USA; RRID: AB_2099233 or RRID: AB_330924) at room temperature for 1 h. Enhanced chemiluminescence reagent (EIR-R307; Elabscience, China) was used for signal detection after secondary antibody incubation. .. Total RNA was isolated from the cells using TRIzol (15596026; Invitrogen, USA) and chloroform (CX1060; Merck, Germany).

    Imaging:

    Article Title: Morpho-Functional Effects of Nonylphenol-Steroid Hormone Co-Exposure on Human Prostate PNT1A Cells.
    Article Snippet: Protein molecular weights were estimated using the Opti-Protein Ultra Marker (cat. no. G623; Applied Biological Materials Inc., Richmond, BC, Canada), a pre-stained protein ladder covering a range from 6.5 to 270 kDa. .. After incubation, protein bands were detected using a chemiluminescent HRP substrate (Cat. No. E-IR-R307, Elabscience Bionovation Inc., Houston, TX, USA) and visualized with a chemiluminescence scanner (ChemiDoc Imaging System, Bio-Rad Laboratories, Hercules, CA, USA). .. Band intensities were quantified using ImageJ 1.54f software (NIH, Bethesda, MD, USA).

    CCK-8 Assay:

    Article Title: Developing a Better Hepatocellular Carcinoma Treatment: AlEu MOF Nanoparticles Enhance Microwave Thermal Ablation and Synergistically Activate the cGAS-STING Pathway
    Article Snippet: Terephthalic acid (1,4-BDC) was supplied by Beijing Bailingwei Technology Co., Ltd. N,N-Dimethylformamide (DMF) and absolute ethanol were provided by Beijing Xiandai Dongfang Technology Development Co., Ltd. High-glucose DMEM was purchased from Thermo Fisher Scientific Inc. Human liver epithelial cells (THLE-2), mouse hepatoma cells (Hepa1-6), trypsin, penicillin–streptomycin, and non-programmable cell freezing medium were obtained from Wuhan Procell Life Science and Technology Co., Ltd. Fetal bovine serum (FBS) was purchased from Shanghai ExCell Biology, Inc. .. The Cell Counting Kit-8 (CCK-8), calcein-acetoxymethyl ester (Calcein-AM)/propidium iodide (PI) double stain kit, Annexin V-FITC/PI apoptosis detection kit, JC-1 mitochondrial membrane potential assay kit, RIPA lysis buffer, phenylmethylsulfonyl fluoride (PMSF) and phosphatase inhibitors, SDS-PAGE precast gels, and ECL chemiluminescent substrate were all obtained from Wuhan Elabscience Biotechnology Co., Ltd. .. The dichloro-dihydro-fluorescein diacetate (DCFH-DA) fluorescent probe, protein prestained marker, and polyvinylidene fluoride (PVDF) membrane were purchased from Shanghai Beyotime Biotechnology Co., Ltd. LabPAGE precast protein gels, protein prestained marker, and skimmed milk powder were supplied by Beijing Lablead Biotechnology Co., Ltd.

    Staining:

    Article Title: Developing a Better Hepatocellular Carcinoma Treatment: AlEu MOF Nanoparticles Enhance Microwave Thermal Ablation and Synergistically Activate the cGAS-STING Pathway
    Article Snippet: Terephthalic acid (1,4-BDC) was supplied by Beijing Bailingwei Technology Co., Ltd. N,N-Dimethylformamide (DMF) and absolute ethanol were provided by Beijing Xiandai Dongfang Technology Development Co., Ltd. High-glucose DMEM was purchased from Thermo Fisher Scientific Inc. Human liver epithelial cells (THLE-2), mouse hepatoma cells (Hepa1-6), trypsin, penicillin–streptomycin, and non-programmable cell freezing medium were obtained from Wuhan Procell Life Science and Technology Co., Ltd. Fetal bovine serum (FBS) was purchased from Shanghai ExCell Biology, Inc. .. The Cell Counting Kit-8 (CCK-8), calcein-acetoxymethyl ester (Calcein-AM)/propidium iodide (PI) double stain kit, Annexin V-FITC/PI apoptosis detection kit, JC-1 mitochondrial membrane potential assay kit, RIPA lysis buffer, phenylmethylsulfonyl fluoride (PMSF) and phosphatase inhibitors, SDS-PAGE precast gels, and ECL chemiluminescent substrate were all obtained from Wuhan Elabscience Biotechnology Co., Ltd. .. The dichloro-dihydro-fluorescein diacetate (DCFH-DA) fluorescent probe, protein prestained marker, and polyvinylidene fluoride (PVDF) membrane were purchased from Shanghai Beyotime Biotechnology Co., Ltd. LabPAGE precast protein gels, protein prestained marker, and skimmed milk powder were supplied by Beijing Lablead Biotechnology Co., Ltd.

    Membrane:

    Article Title: Developing a Better Hepatocellular Carcinoma Treatment: AlEu MOF Nanoparticles Enhance Microwave Thermal Ablation and Synergistically Activate the cGAS-STING Pathway
    Article Snippet: Terephthalic acid (1,4-BDC) was supplied by Beijing Bailingwei Technology Co., Ltd. N,N-Dimethylformamide (DMF) and absolute ethanol were provided by Beijing Xiandai Dongfang Technology Development Co., Ltd. High-glucose DMEM was purchased from Thermo Fisher Scientific Inc. Human liver epithelial cells (THLE-2), mouse hepatoma cells (Hepa1-6), trypsin, penicillin–streptomycin, and non-programmable cell freezing medium were obtained from Wuhan Procell Life Science and Technology Co., Ltd. Fetal bovine serum (FBS) was purchased from Shanghai ExCell Biology, Inc. .. The Cell Counting Kit-8 (CCK-8), calcein-acetoxymethyl ester (Calcein-AM)/propidium iodide (PI) double stain kit, Annexin V-FITC/PI apoptosis detection kit, JC-1 mitochondrial membrane potential assay kit, RIPA lysis buffer, phenylmethylsulfonyl fluoride (PMSF) and phosphatase inhibitors, SDS-PAGE precast gels, and ECL chemiluminescent substrate were all obtained from Wuhan Elabscience Biotechnology Co., Ltd. .. The dichloro-dihydro-fluorescein diacetate (DCFH-DA) fluorescent probe, protein prestained marker, and polyvinylidene fluoride (PVDF) membrane were purchased from Shanghai Beyotime Biotechnology Co., Ltd. LabPAGE precast protein gels, protein prestained marker, and skimmed milk powder were supplied by Beijing Lablead Biotechnology Co., Ltd.

    Lysis:

    Article Title: Developing a Better Hepatocellular Carcinoma Treatment: AlEu MOF Nanoparticles Enhance Microwave Thermal Ablation and Synergistically Activate the cGAS-STING Pathway
    Article Snippet: Terephthalic acid (1,4-BDC) was supplied by Beijing Bailingwei Technology Co., Ltd. N,N-Dimethylformamide (DMF) and absolute ethanol were provided by Beijing Xiandai Dongfang Technology Development Co., Ltd. High-glucose DMEM was purchased from Thermo Fisher Scientific Inc. Human liver epithelial cells (THLE-2), mouse hepatoma cells (Hepa1-6), trypsin, penicillin–streptomycin, and non-programmable cell freezing medium were obtained from Wuhan Procell Life Science and Technology Co., Ltd. Fetal bovine serum (FBS) was purchased from Shanghai ExCell Biology, Inc. .. The Cell Counting Kit-8 (CCK-8), calcein-acetoxymethyl ester (Calcein-AM)/propidium iodide (PI) double stain kit, Annexin V-FITC/PI apoptosis detection kit, JC-1 mitochondrial membrane potential assay kit, RIPA lysis buffer, phenylmethylsulfonyl fluoride (PMSF) and phosphatase inhibitors, SDS-PAGE precast gels, and ECL chemiluminescent substrate were all obtained from Wuhan Elabscience Biotechnology Co., Ltd. .. The dichloro-dihydro-fluorescein diacetate (DCFH-DA) fluorescent probe, protein prestained marker, and polyvinylidene fluoride (PVDF) membrane were purchased from Shanghai Beyotime Biotechnology Co., Ltd. LabPAGE precast protein gels, protein prestained marker, and skimmed milk powder were supplied by Beijing Lablead Biotechnology Co., Ltd.

    SDS Page:

    Article Title: Developing a Better Hepatocellular Carcinoma Treatment: AlEu MOF Nanoparticles Enhance Microwave Thermal Ablation and Synergistically Activate the cGAS-STING Pathway
    Article Snippet: Terephthalic acid (1,4-BDC) was supplied by Beijing Bailingwei Technology Co., Ltd. N,N-Dimethylformamide (DMF) and absolute ethanol were provided by Beijing Xiandai Dongfang Technology Development Co., Ltd. High-glucose DMEM was purchased from Thermo Fisher Scientific Inc. Human liver epithelial cells (THLE-2), mouse hepatoma cells (Hepa1-6), trypsin, penicillin–streptomycin, and non-programmable cell freezing medium were obtained from Wuhan Procell Life Science and Technology Co., Ltd. Fetal bovine serum (FBS) was purchased from Shanghai ExCell Biology, Inc. .. The Cell Counting Kit-8 (CCK-8), calcein-acetoxymethyl ester (Calcein-AM)/propidium iodide (PI) double stain kit, Annexin V-FITC/PI apoptosis detection kit, JC-1 mitochondrial membrane potential assay kit, RIPA lysis buffer, phenylmethylsulfonyl fluoride (PMSF) and phosphatase inhibitors, SDS-PAGE precast gels, and ECL chemiluminescent substrate were all obtained from Wuhan Elabscience Biotechnology Co., Ltd. .. The dichloro-dihydro-fluorescein diacetate (DCFH-DA) fluorescent probe, protein prestained marker, and polyvinylidene fluoride (PVDF) membrane were purchased from Shanghai Beyotime Biotechnology Co., Ltd. LabPAGE precast protein gels, protein prestained marker, and skimmed milk powder were supplied by Beijing Lablead Biotechnology Co., Ltd.



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