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3 3 5 5 tetramethylbenzidine substrate tmb  (tiangen biotech co)


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    tiangen biotech co 3 3 5 5 tetramethylbenzidine substrate tmb
    3 3 5 5 Tetramethylbenzidine Substrate Tmb, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tetramethylbenzidine+substrate+solution/Soluble+TMB+Substrate+Solution/pm41843376-172-9-12
    Average 99 stars, based on 15 article reviews
    3 3 5 5 tetramethylbenzidine substrate tmb - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Anti-PD1 antibodies and their use as therapeutics and diagnostics
    Article Snippet: .. The HRP-linked anti-mouse IgG antibody (Cat. No. 7076S, Cell Signaling Technology, USA and Shanghai, China) and chemiluminescent reagent (Cat. No. PA107-01, TIANGEN, China) were used to detect and develop the ELISA signal, which were read out by a plate reader (PHREAstar FS, BMG LABTECH, Germany) at wavelength of 450 nm. ..

    Article Title: Proteinaceous heterodimer and use thereof
    Article Snippet: .. Briefly, ELISA plates were coated with anti-human IL10 (BioLegend, Lot.NO:B179948) at 5 μg/ml dissolved in buffer (50 mM Na2CO3/NaHCO3; pH9.6), overnight at 4° C. The plates were washed for three times with PBST (PH7.4) containing 0.05% (V/V) Tween-20 and blocked with 3% BSA in PBS for 1 h, then, Erb-(huIL10)2-6-9 that was 2-fold serial diluted from 2000 ng/ml was added and incubated for 2 h at 37° C. Then 2 μg/ml EGFR-Fc-biotin was added and incubated for 1 h at 37° C. After that, 1:1000 diluted SA-HRP was added and incubated for 40 min at 37° C. The binding was examined with the (TMB, TIANGEN Cat #PA107-01; Lot #1614) substrate, stopped with 2M H2SO4. .. The absorbance at 450 nm-650 nm was detected in a Molecular Devices SpectraMax Plus-384 microplate reader.

    Article Title: Proteinaceous heterodimer and use thereof
    Article Snippet: .. Briefly, ELISA plates were coated with EGFR-huFc at 2 μg/ml dissolved in buffer (50 mM Na2CO3/NaHCO3; pH9.6), overnight at 4° C. The plates were washed for three times with PBST (PH7.4) containing 0.05% (V/V) Tween-20 and blocked with 3% BSA in PBS for 1 h, then, serial diluted Erb-huIFNa2-6-9 was added and incubated for 2 h at 37° C. Then 1 μg/ml anti-huIFNa-biotin was added and incubated for 1 h at 37° C. Subsequently, 1:1000 diluted SA-HRP was added and incubated for 40 min at 37° C. The binding was examined with the (TMB, TIANGEN Cat #PA107-01; Lot #1614) substrate, stopped with 2M H2SO4. .. The absorbance at 450 nm-650 nm was examined in a Molecular Devices SpectraMax Plus-384 microplate reader.

    Incubation:

    Article Title: Proteinaceous heterodimer and use thereof
    Article Snippet: .. Briefly, ELISA plates were coated with anti-human IL10 (BioLegend, Lot.NO:B179948) at 5 μg/ml dissolved in buffer (50 mM Na2CO3/NaHCO3; pH9.6), overnight at 4° C. The plates were washed for three times with PBST (PH7.4) containing 0.05% (V/V) Tween-20 and blocked with 3% BSA in PBS for 1 h, then, Erb-(huIL10)2-6-9 that was 2-fold serial diluted from 2000 ng/ml was added and incubated for 2 h at 37° C. Then 2 μg/ml EGFR-Fc-biotin was added and incubated for 1 h at 37° C. After that, 1:1000 diluted SA-HRP was added and incubated for 40 min at 37° C. The binding was examined with the (TMB, TIANGEN Cat #PA107-01; Lot #1614) substrate, stopped with 2M H2SO4. .. The absorbance at 450 nm-650 nm was detected in a Molecular Devices SpectraMax Plus-384 microplate reader.

    Article Title: Development of a core streptavidin-bridging amplified ELISA based on p54 epitope for high-sensitivity detection of african swine fever virus antibodies.
    Article Snippet: Infection with the African swine fever virus (ASFV) causes a highly acute and lethal disease in pigs, emphasizing the urgent need for early detection to manage outbreaks effectively.. While indirect enzyme-linked immunosorbent assays (ELISAs) based on antigenic epitopes provide high sensitivity, conventional adsorption of epitopes onto ELISA plates can hinder antibody recognition.. In this study, we developed an indirect ELISA for ASFV antibody detection by integrating the identified p54 antigenic epitope into a core-streptavidin (cSA)-based immobilization system.

    Article Title: A potent human neutralizing antibody targeting the receptor-binding site in the glycoprotein gp350 of Epstein-Barr virus.
    Article Snippet: 1Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing 100084, China 2Yangshengtang Co., Ltd., Hangzhou 310007, China 3Department of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat‐sen University, Shenzhen 518033, China 4Cryo-electron Microscopy Center, Southern University of Science and Technology, Shenzhen 518055, China 5Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China 6State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Department of Experimental Research, Sun Yat-sen University Cancer Center, Sun Yat-sen University, Guangzhou 510060, China 7Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China 8Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 518132, China

    Article Title: Proteinaceous heterodimer and use thereof
    Article Snippet: .. Briefly, ELISA plates were coated with EGFR-huFc at 2 μg/ml dissolved in buffer (50 mM Na2CO3/NaHCO3; pH9.6), overnight at 4° C. The plates were washed for three times with PBST (PH7.4) containing 0.05% (V/V) Tween-20 and blocked with 3% BSA in PBS for 1 h, then, serial diluted Erb-huIFNa2-6-9 was added and incubated for 2 h at 37° C. Then 1 μg/ml anti-huIFNa-biotin was added and incubated for 1 h at 37° C. Subsequently, 1:1000 diluted SA-HRP was added and incubated for 40 min at 37° C. The binding was examined with the (TMB, TIANGEN Cat #PA107-01; Lot #1614) substrate, stopped with 2M H2SO4. .. The absorbance at 450 nm-650 nm was examined in a Molecular Devices SpectraMax Plus-384 microplate reader.

    Binding Assay:

    Article Title: Proteinaceous heterodimer and use thereof
    Article Snippet: .. Briefly, ELISA plates were coated with anti-human IL10 (BioLegend, Lot.NO:B179948) at 5 μg/ml dissolved in buffer (50 mM Na2CO3/NaHCO3; pH9.6), overnight at 4° C. The plates were washed for three times with PBST (PH7.4) containing 0.05% (V/V) Tween-20 and blocked with 3% BSA in PBS for 1 h, then, Erb-(huIL10)2-6-9 that was 2-fold serial diluted from 2000 ng/ml was added and incubated for 2 h at 37° C. Then 2 μg/ml EGFR-Fc-biotin was added and incubated for 1 h at 37° C. After that, 1:1000 diluted SA-HRP was added and incubated for 40 min at 37° C. The binding was examined with the (TMB, TIANGEN Cat #PA107-01; Lot #1614) substrate, stopped with 2M H2SO4. .. The absorbance at 450 nm-650 nm was detected in a Molecular Devices SpectraMax Plus-384 microplate reader.

    Article Title: Anti-PD-L1 antibodies and their use as therapeutics and diagnostics
    Article Snippet: .. After blocking with 3% bovine serum albumin in TBST (20 mM Tris, 150 mM NaCl, 0.05% Tween20, pH7.5) and incubating with culture supernatants of hybridoma clones, the HRP-conjugated horse anti-mouse IgG antibody (Cat. No. 7076S, Cell Signaling Technology) and tetramethylbenzidine (TMB) (Cat. No. PA107-01, TianGen, Beijing, China) were used to detect binding signals by a plate reader (PHREAstar, BMG Labtech, Germany) as light absorbance at 450 nm. .. The ELISA-positive clones were further verified by fluorescence-activated cell sorting (FACS).

    Article Title: Proteinaceous heterodimer and use thereof
    Article Snippet: .. Briefly, ELISA plates were coated with EGFR-huFc at 2 μg/ml dissolved in buffer (50 mM Na2CO3/NaHCO3; pH9.6), overnight at 4° C. The plates were washed for three times with PBST (PH7.4) containing 0.05% (V/V) Tween-20 and blocked with 3% BSA in PBS for 1 h, then, serial diluted Erb-huIFNa2-6-9 was added and incubated for 2 h at 37° C. Then 1 μg/ml anti-huIFNa-biotin was added and incubated for 1 h at 37° C. Subsequently, 1:1000 diluted SA-HRP was added and incubated for 40 min at 37° C. The binding was examined with the (TMB, TIANGEN Cat #PA107-01; Lot #1614) substrate, stopped with 2M H2SO4. .. The absorbance at 450 nm-650 nm was examined in a Molecular Devices SpectraMax Plus-384 microplate reader.

    Blocking Assay:

    Article Title: Anti-PD-L1 antibodies and their use as therapeutics and diagnostics
    Article Snippet: .. After blocking with 3% bovine serum albumin in TBST (20 mM Tris, 150 mM NaCl, 0.05% Tween20, pH7.5) and incubating with culture supernatants of hybridoma clones, the HRP-conjugated horse anti-mouse IgG antibody (Cat. No. 7076S, Cell Signaling Technology) and tetramethylbenzidine (TMB) (Cat. No. PA107-01, TianGen, Beijing, China) were used to detect binding signals by a plate reader (PHREAstar, BMG Labtech, Germany) as light absorbance at 450 nm. .. The ELISA-positive clones were further verified by fluorescence-activated cell sorting (FACS).

    Clone Assay:

    Article Title: Anti-PD-L1 antibodies and their use as therapeutics and diagnostics
    Article Snippet: .. After blocking with 3% bovine serum albumin in TBST (20 mM Tris, 150 mM NaCl, 0.05% Tween20, pH7.5) and incubating with culture supernatants of hybridoma clones, the HRP-conjugated horse anti-mouse IgG antibody (Cat. No. 7076S, Cell Signaling Technology) and tetramethylbenzidine (TMB) (Cat. No. PA107-01, TianGen, Beijing, China) were used to detect binding signals by a plate reader (PHREAstar, BMG Labtech, Germany) as light absorbance at 450 nm. .. The ELISA-positive clones were further verified by fluorescence-activated cell sorting (FACS).



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