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tprofessional standard thermal cycler with temperature gradient function  (Biometra)

 
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    Structured Review

    Biometra tprofessional standard thermal cycler with temperature gradient function
    Tprofessional Standard Thermal Cycler With Temperature Gradient Function, supplied by Biometra, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/temperature+gradient+function/thermal+cycler+biometra/pm24024645-99-11-10
    Average 90 stars, based on 1 article reviews
    tprofessional standard thermal cycler with temperature gradient function - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Conservation of Verbascum sinaiticum Benth using innovative tissue culture technique and DNA barcoding
    Article Snippet: The standard plant barcodes, matK and rbcL , which are specific regions of two plastids, were amplified separately using a Biometra Tone 96G thermal cycler (Analytik Jena GmbH, Germany), For the 25 μL total volume PCR reactions, the following ingredients were utilized: Flexi DNA polymerase GoTaq ® G2 (Promega, USA).

    Article Title: Morpho-cultural and molecular characterization of trichoderma species from the northwestern himalayan apple rhizosphere of India
    Article Snippet: Polymerase chain reaction (PCR) using ITS region, and TEF 1-α , and RPB2 gene-specific markers was performed in 0.2 ml PCR tubes in a T-Gradient thermal cycler (Whatman Biometra, T-Gradient, Goettingen, Germany) using 40–50 ng of genomic DNA of each isolate in a final volume of 50μl reaction mixture comprised of 31.6μl distilled sterilized PCR-water, 5.0μl (1.0X) PCR buffer, 3μl (1.5 mM) MgCl 2) , 4μl (0.2 mM) DNTPs, 2.0μl of each forward and reverse primers (0.4 pmol) and 0.4μl Taq polymerase (1.0 unit) provided by Thermo Fisher Scientific Pvt.

    Polymerase Chain Reaction:

    Article Title: Selected Pathway Analyses to Gain Mechanistic Insights into the Pathogenesis of Feline Hypertrophic Cardiomyopathy.
    Article Snippet: The master mix consisted of 10 μL TaqManTM Fast Universal PCR Master Mix (2×), no AmpEraseTM UNG (Thermo Fisher Scientific, USA), 0.9 μL per primer (10 μM), and 6.2 μL water of molecular biology grade, resulting in a final volume of 20 μL after addition of 2 μL sample cDNA (30 ng of DNA). .. Samples were run on a Biometra TRIO Combi Thermocycler under the following thermal profile: (1) initial denaturation at 95 ◦C for 20 s; (2) denaturation at 95 ◦C for 3 s; and (3) annealing at either 60 ◦C, 61.8 ◦C, 63 ◦C or 64 ◦C, for 30 s based on the optimal PCR annealing temperatures determined during the primer design. ..

    Article Title: A novel C-type lectin gene with QPS and WND motifs in clam Meretrix meretrix.
    Article Snippet: .. The gene-specific primers set, MmCTL5-F and MmCTL5-R (Table 1), were used to amplify the open reading frame (ORF) of MmCTL5 by a PCR Thermal Cycler Biometra TAdvanced 96 SG (Analytik Jena AG, Germany). .. The PCR products were gel-purified and attached to the cloning pMD18-T vector (6011, TaKaRa, Japan).

    Reverse Transcription:

    Article Title: Evaluation of Commercially Available Kits for Parallel DNA and microRNA Isolation Suitable for Epigenetic Analyses from Cell-Free Saliva and Salivary Extracellular Vesicles.
    Article Snippet: .. The standard row RNA—and saliva RNA samples were reverse transcribed to cDNA using the TaqMan MicroRNA Reverse Transcription Kit (Applied BiosystemsTM, Thermo Fisher Scientific, Waltham, MA, USA) on a T3000 Thermocycler (Biometra GmbH, Göttingen, Germany) before being applied to qPCR. .. All qPCRs were performed on 384-well plates using a LightCycler® 480 (Roche, Basel, Switzerland).

    Real-time Polymerase Chain Reaction:

    Article Title: Evaluation of Commercially Available Kits for Parallel DNA and microRNA Isolation Suitable for Epigenetic Analyses from Cell-Free Saliva and Salivary Extracellular Vesicles.
    Article Snippet: .. The standard row RNA—and saliva RNA samples were reverse transcribed to cDNA using the TaqMan MicroRNA Reverse Transcription Kit (Applied BiosystemsTM, Thermo Fisher Scientific, Waltham, MA, USA) on a T3000 Thermocycler (Biometra GmbH, Göttingen, Germany) before being applied to qPCR. .. All qPCRs were performed on 384-well plates using a LightCycler® 480 (Roche, Basel, Switzerland).



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