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tbe 1x buffer  (Biotium)


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    Structured Review

    Biotium tbe 1x buffer
    Tbe 1x Buffer, supplied by Biotium, used in various techniques. Bioz Stars score: 96/100, based on 598 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tbe+buffer/TBE+Buffer/pm41820566-252-10-22
    Average 96 stars, based on 598 article reviews
    tbe 1x buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: DNA Nanoarray for Multiplexed RNA Detection With Single-Molecule Readout.
    Article Snippet: .. Electrophoresis was performed in 0.5 × TBE buffer (44.5 m m Tris, 44.5 m m boric acid, 1.0 m m EDTA, pH 8.0) at 110 V for 1.5 h. Gels were stained with GelRed (Biotium, Inc.) for 20 min and visualized using a UV transilluminator. ..

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Article Title: Strong Cross-Reactivity of Anti-Plasmodium falciparum pLDH Monoclonal Antibodies With Plasmodium malariae pLDH in the Abbott Bioline Malaria Ag P.f/P.f/P.v Rapid Diagnostic Test.
    Article Snippet: .. The fragments obtained were separated by electrophoresis in 1.5% agarose gel in TBE buffer and visualised with GelRed (Biotium, Fremont, USA) through UV light with a molecular weight marker of 100 bp [13]. .. The Abbott Bioline Malaria Ag P.f/P.f/P.v RDTs (05FK120- 25 T/ Kit and 05FK123- POCT) were applied to samples, according to the manufacturer instructions.

    Staining:

    Article Title: DNA Nanoarray for Multiplexed RNA Detection With Single-Molecule Readout.
    Article Snippet: .. Electrophoresis was performed in 0.5 × TBE buffer (44.5 m m Tris, 44.5 m m boric acid, 1.0 m m EDTA, pH 8.0) at 110 V for 1.5 h. Gels were stained with GelRed (Biotium, Inc.) for 20 min and visualized using a UV transilluminator. ..

    Article Title: Aging-associated endolysosomal decline drives inflammaging and neurodegeneration through the STING-IFN-I axis
    Article Snippet: After phenol/chloroform extraction, the aqueous supernatant was incubated with 500 μg/ml DNase-free RNase A (QIAGEN) for 30 min at 37°C, again followed by phenol/chloroform extraction. .. The DNA-containing aqueous phase was precipitated, resuspended in TE buffer, DNA concentrations were adjusted to the protein concentration of cytoplasmic fractions and analyzed by a 2% (w/v) agarose gel in 1 × TBE buffer with 1 × GelRed stain (Biotium, Madison, WI) incorporated in gel. .. Total RNA was isolated from tissues and cells using either EZNA HP RNA isolation kit (Omega, #R6812-02) or RNeasy Mini kit (Qiagen, #74104) or Trizol (Invitrogen, #15596026) according to manufacturer instructions.

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Article Title: Evaluation of marine yeasts Beta-glucans in the growth performance, defense and antioxidant response systems in L. vannamei in comparison to model yeast S. cerevisiae Beta-glucans.
    Article Snippet: One of the most promising methods to increase shrimp resistance to pathogens is by stimulating shrimp defense cells using immune-stimulating molecules such as beta-glucans (BGs).. Although yeast BGs have offered excellent results, most studies are based on the model yeast, S. cerevisiae.. However, marine yeasts could offer an advantage over terrestrial yeasts due to the extreme conditions in which they live.

    Article Title: Electrochemical sensor for miRNA-34a detection: Synergistic signal amplification for robust and sensitive analysis
    Article Snippet: This study developed an electrochemical biosensor for the detection of miRNA-34a by integrating a synergistic signal amplification strategy that combined the electrocatalytic properties of reduced graphene oxide (rGO) with the enzymatic activity of double-stranded specific nuclease (DSN), while employing magnetic separation technology to ensure robust interference resistance.. The biosensor was constructed by electrodepositing rGO onto a glassy carbon electrode via cyclic voltammetry (CV), utilizing its electrocatalytic activity to enhance the electrochemical response.. Simultaneously, the presence of target miRNA-34a initiated a DSN-mediated enzymatic cyclic reaction, resulting in secondary signal amplification.

    Article Title: Honey environmental DNA reveals entomological fingerprints through dual mitochondrial cytochrome c oxidase subunit 1 (COI) and cytochrome b (CYTB) metabarcoding.
    Article Snippet: .. PCR amplification consisted of an initial denaturation at 95 °C for 5 min, followed by 35 cycles of 95 °C for 30 s, annealing for 30 s at the temperatures specified below, and 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR products were visualized after 2.5% agarose gel electrophoresis in TBE 1X buffer and 1X GelRed Nucleic Acid Gel Stain (Biotium Inc., Hayward, CA, USA). ..

    Protein Concentration:

    Article Title: Aging-associated endolysosomal decline drives inflammaging and neurodegeneration through the STING-IFN-I axis
    Article Snippet: After phenol/chloroform extraction, the aqueous supernatant was incubated with 500 μg/ml DNase-free RNase A (QIAGEN) for 30 min at 37°C, again followed by phenol/chloroform extraction. .. The DNA-containing aqueous phase was precipitated, resuspended in TE buffer, DNA concentrations were adjusted to the protein concentration of cytoplasmic fractions and analyzed by a 2% (w/v) agarose gel in 1 × TBE buffer with 1 × GelRed stain (Biotium, Madison, WI) incorporated in gel. .. Total RNA was isolated from tissues and cells using either EZNA HP RNA isolation kit (Omega, #R6812-02) or RNeasy Mini kit (Qiagen, #74104) or Trizol (Invitrogen, #15596026) according to manufacturer instructions.

    Agarose Gel Electrophoresis:

    Article Title: Aging-associated endolysosomal decline drives inflammaging and neurodegeneration through the STING-IFN-I axis
    Article Snippet: After phenol/chloroform extraction, the aqueous supernatant was incubated with 500 μg/ml DNase-free RNase A (QIAGEN) for 30 min at 37°C, again followed by phenol/chloroform extraction. .. The DNA-containing aqueous phase was precipitated, resuspended in TE buffer, DNA concentrations were adjusted to the protein concentration of cytoplasmic fractions and analyzed by a 2% (w/v) agarose gel in 1 × TBE buffer with 1 × GelRed stain (Biotium, Madison, WI) incorporated in gel. .. Total RNA was isolated from tissues and cells using either EZNA HP RNA isolation kit (Omega, #R6812-02) or RNeasy Mini kit (Qiagen, #74104) or Trizol (Invitrogen, #15596026) according to manufacturer instructions.

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Article Title: Evaluation of marine yeasts Beta-glucans in the growth performance, defense and antioxidant response systems in L. vannamei in comparison to model yeast S. cerevisiae Beta-glucans.
    Article Snippet: One of the most promising methods to increase shrimp resistance to pathogens is by stimulating shrimp defense cells using immune-stimulating molecules such as beta-glucans (BGs).. Although yeast BGs have offered excellent results, most studies are based on the model yeast, S. cerevisiae.. However, marine yeasts could offer an advantage over terrestrial yeasts due to the extreme conditions in which they live.

    Article Title: Electrochemical sensor for miRNA-34a detection: Synergistic signal amplification for robust and sensitive analysis
    Article Snippet: This study developed an electrochemical biosensor for the detection of miRNA-34a by integrating a synergistic signal amplification strategy that combined the electrocatalytic properties of reduced graphene oxide (rGO) with the enzymatic activity of double-stranded specific nuclease (DSN), while employing magnetic separation technology to ensure robust interference resistance.. The biosensor was constructed by electrodepositing rGO onto a glassy carbon electrode via cyclic voltammetry (CV), utilizing its electrocatalytic activity to enhance the electrochemical response.. Simultaneously, the presence of target miRNA-34a initiated a DSN-mediated enzymatic cyclic reaction, resulting in secondary signal amplification.

    Article Title: Strong Cross-Reactivity of Anti-Plasmodium falciparum pLDH Monoclonal Antibodies With Plasmodium malariae pLDH in the Abbott Bioline Malaria Ag P.f/P.f/P.v Rapid Diagnostic Test.
    Article Snippet: .. The fragments obtained were separated by electrophoresis in 1.5% agarose gel in TBE buffer and visualised with GelRed (Biotium, Fremont, USA) through UV light with a molecular weight marker of 100 bp [13]. .. The Abbott Bioline Malaria Ag P.f/P.f/P.v RDTs (05FK120- 25 T/ Kit and 05FK123- POCT) were applied to samples, according to the manufacturer instructions.

    Article Title: Honey environmental DNA reveals entomological fingerprints through dual mitochondrial cytochrome c oxidase subunit 1 (COI) and cytochrome b (CYTB) metabarcoding.
    Article Snippet: .. PCR amplification consisted of an initial denaturation at 95 °C for 5 min, followed by 35 cycles of 95 °C for 30 s, annealing for 30 s at the temperatures specified below, and 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR products were visualized after 2.5% agarose gel electrophoresis in TBE 1X buffer and 1X GelRed Nucleic Acid Gel Stain (Biotium Inc., Hayward, CA, USA). ..

    Marker:

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Article Title: Strong Cross-Reactivity of Anti-Plasmodium falciparum pLDH Monoclonal Antibodies With Plasmodium malariae pLDH in the Abbott Bioline Malaria Ag P.f/P.f/P.v Rapid Diagnostic Test.
    Article Snippet: .. The fragments obtained were separated by electrophoresis in 1.5% agarose gel in TBE buffer and visualised with GelRed (Biotium, Fremont, USA) through UV light with a molecular weight marker of 100 bp [13]. .. The Abbott Bioline Malaria Ag P.f/P.f/P.v RDTs (05FK120- 25 T/ Kit and 05FK123- POCT) were applied to samples, according to the manufacturer instructions.

    Molecular Weight:

    Article Title: Strong Cross-Reactivity of Anti-Plasmodium falciparum pLDH Monoclonal Antibodies With Plasmodium malariae pLDH in the Abbott Bioline Malaria Ag P.f/P.f/P.v Rapid Diagnostic Test.
    Article Snippet: .. The fragments obtained were separated by electrophoresis in 1.5% agarose gel in TBE buffer and visualised with GelRed (Biotium, Fremont, USA) through UV light with a molecular weight marker of 100 bp [13]. .. The Abbott Bioline Malaria Ag P.f/P.f/P.v RDTs (05FK120- 25 T/ Kit and 05FK123- POCT) were applied to samples, according to the manufacturer instructions.

    Polymerase Chain Reaction:

    Article Title: Honey environmental DNA reveals entomological fingerprints through dual mitochondrial cytochrome c oxidase subunit 1 (COI) and cytochrome b (CYTB) metabarcoding.
    Article Snippet: .. PCR amplification consisted of an initial denaturation at 95 °C for 5 min, followed by 35 cycles of 95 °C for 30 s, annealing for 30 s at the temperatures specified below, and 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR products were visualized after 2.5% agarose gel electrophoresis in TBE 1X buffer and 1X GelRed Nucleic Acid Gel Stain (Biotium Inc., Hayward, CA, USA). ..

    Amplification:

    Article Title: Honey environmental DNA reveals entomological fingerprints through dual mitochondrial cytochrome c oxidase subunit 1 (COI) and cytochrome b (CYTB) metabarcoding.
    Article Snippet: .. PCR amplification consisted of an initial denaturation at 95 °C for 5 min, followed by 35 cycles of 95 °C for 30 s, annealing for 30 s at the temperatures specified below, and 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR products were visualized after 2.5% agarose gel electrophoresis in TBE 1X buffer and 1X GelRed Nucleic Acid Gel Stain (Biotium Inc., Hayward, CA, USA). ..



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