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Promega superscript kit
Superscript Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscripttm+iv+reverse+transcriptase+kit/superscript+kit/pmc02677865-75-22-24
Average 90 stars, based on 1 article reviews
superscript kit - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Method of treating skin with ingenol mebutate
Article Snippet: To denature the RNA, reaction tubes were placed in a GeneAmp PCR System 9700 (Applied Biosystems, Cheshire, U.K.) and heated for 5 min/95° C., prior to cooling to 4° C. Superscript (1 μl) and RNAsin® Ribonuclease Inhibitor (1 μl, Promega, Hampshire, U.K.) were added to each tube, and the tubes subjected to 30 cycles at 20° C. for 10 min, 42° C. for 1 h and 95° C. for 5 min, prior to storage at 4° C., until required.

Article Title: The Nrf2/PGC1 α Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer.
Article Snippet: The reverse transcription reaction was performed using a SuperScript RT-PCR kit (Promega), and RT-qPCR was performed using the MX3000P instrument (Agilent, USA). mRNA levels were normalized to those of β-actin.

Article Title: A lncRNA from the FTO locus acts as a suppressor of the m 6 A writer complex and p53 tumor suppression signaling.
Article Snippet: Quantitative PCR was performed using SYBR Green Master mix Kit (Bio-Rad) in Bio-Rad CFXmanager 3.1.

Article Title: The Nrf2/PGC1 α Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer
Article Snippet: The reverse transcription reaction was performed using a SuperScript RT-PCR kit (Promega), and RT-qPCR was performed using the MX3000P instrument (Agilent, USA). mRNA levels were normalized to those of β -actin.

Reverse Transcription:

Article Title: Tussilagone Reduces Tumorigenesis by Diminishing Inflammation in Experimental Colitis-Associated Colon Cancer
Article Snippet: Total RNA from the colon homogenates was isolated with an RNAeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. After RNA preparation, cDNA was transcribed using a single reverse transcriptase synthetic step with Superscript reagents (Promega, Madison, WI, USA). .. The cDNA was used for quantitative PCR analyses using GoTaq ® qPCR Master Mix for Dye-Based Detection (Promega, Madison, WI) and LightCycler ® Nano (Roche, Basel, Switzerland).

Isolation:

Article Title: Par-4 regulates autophagic cell death in human cancer cells via upregulating p53 and BNIP3.
Article Snippet: .. RNA was isolated from cells using RNeasy Mini kits (Qiagen, Valencia, CA, USA). cDNA was synthesized using SuperScript kit (Promega). .. The following primer pairs (Integrated DNA Technology, USA) were used for real-time PCR amplification: Human Par-4: 5ʹ-GATCCCGAGATTTCCGGCAG-3ʹ (forward) 5ʹ-GTTGGGCCTCCTCCAGATAC-3ʹ (reverse) Human BNIP3: 5ʹ-CAATAATGGGAACGGGGGCA-3ʹ (Forward) 5ʹ-AGCTCTTGGAGCTACTCCGT-3’ (Reverse) Human GAPDH 5ʹ-ATGGGGAAGGTGAAGGTCG-3ʹ (forward) 5ʹ-GGGGTCATTGATGGCAACAATA-3ʹ (reverse) Journal Pre-proof Jo ur na l P re -p ro of 11 Quantitative real-time PCR was carried out using PowerUpTM SYBR® Green Master Mix (Applied Biosystems) on QuantStudioTM 7 flux real time PCR system (Applied Biosystems).

Synthesized:

Article Title: Par-4 regulates autophagic cell death in human cancer cells via upregulating p53 and BNIP3.
Article Snippet: .. RNA was isolated from cells using RNeasy Mini kits (Qiagen, Valencia, CA, USA). cDNA was synthesized using SuperScript kit (Promega). .. The following primer pairs (Integrated DNA Technology, USA) were used for real-time PCR amplification: Human Par-4: 5ʹ-GATCCCGAGATTTCCGGCAG-3ʹ (forward) 5ʹ-GTTGGGCCTCCTCCAGATAC-3ʹ (reverse) Human BNIP3: 5ʹ-CAATAATGGGAACGGGGGCA-3ʹ (Forward) 5ʹ-AGCTCTTGGAGCTACTCCGT-3’ (Reverse) Human GAPDH 5ʹ-ATGGGGAAGGTGAAGGTCG-3ʹ (forward) 5ʹ-GGGGTCATTGATGGCAACAATA-3ʹ (reverse) Journal Pre-proof Jo ur na l P re -p ro of 11 Quantitative real-time PCR was carried out using PowerUpTM SYBR® Green Master Mix (Applied Biosystems) on QuantStudioTM 7 flux real time PCR system (Applied Biosystems).



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