superscript vilo cdna synthesis kit (Thermo Fisher)

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Thermo Fisher superscript vilo cdna synthesis kit
Superscript Vilo Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscripttm+iv+reverse+transcriptase+kit/Stabilized+Blood-to-CT+Nucleic+Acid+Preparation+Kit+for+qPCR%2C+compatible+with+PAXgene+Blood+RNA+Tubes/pmc03991476-70-10-9
Average 95 stars, based on 287 article reviews

superscript vilo cdna synthesis kit - by Bioz Stars, 2026-09

95/100 stars

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Gene Expression:

Article Title: Cellular iron governs the host response to malaria
Article Snippet: Gene expression analysis by quantitative real-time PCR, was performed on liver samples preserved in RNAlater Stabilization Solution and stored at −80° C (AM7020, Thermo Fisher Scientific). .. The tissue was homogenised with a TissueRuptor II (9002725, QIAGEN) before total RNA was extracted using the RNeasy Plus Mini Kit (74136, QIAGEN), according to the manufacturer’s protocols. cDNA was synthesised using the High-Capacity RNA-to-cDNA Kit (4387406, Thermo Fisher Scientific) and subsequent gene expression analysis was performed on 1-5 ng/mL cDNA, using TaqMan Gene Expression Master Mix (4369016, Thermo Fisher Scientific) and the TaqMan Gene Expression Assays (Thermo Fisher Scientific) listed in , all according to the manufacturers’ instructions. .. An Applied Biosystems 6500 Fast Real-Time PCR System (Thermo Fisher Scientific) instrument was used to run the samples, and the relative gene expression was calculated through the 2 -ΔCT method ( ).

Reverse Transcription:

Article Title: The biological activity of bacterial rhamnolipids is linked to their molecular structure
Article Snippet: The root system was sampled 1 hour and 48 hours after transplanting and transferred into a 1.5 ml Eppendorf tube for immediate freezing in liquid nitrogen and subsequent storage at −80°C until further use. .. Root total RNA was extracted with the RNeasy Plant Mini Kit (QIAGEN, Germany), genomic DNA contamination was removed with TURBO DNA-freeTM Kit (Invitrogen, Germany) und reverse transcription was performed with the High-Capacity cDNA Reverse Transcription Kit (Thermofisher, Germany) and 100 ng RNA per reaction (Thermofisher) according to the respective manufacturer’s instructions. .. RT-PCR implied 20 μl per reaction with 10 μl 2X SYBRTM Green PCR Master Mix (Thermofisher), 0.5 μl of forward and reverse primer each (10 μM) and 1 μl cDNA (diluted 1:5) filled up with molecular grade water (supplementary table 1).

Article Title: Murine and Human Gastric Tissue Establishes Organoids after 48 Hours of Cold Ischemia Time during Shipment
Article Snippet: .. Isolated RNA was stored at minus 80 °C with 0.2 units/μL SUPERASE-In RNase inhibitor (Invitrogen, AM2694). mRNA was reverse-transcribed into cDNA using 100 ng mRNA with a High-Capacity RNA-to-cDNA Kit (Applied Biosystems, 4387406). .. Quantitative real-time PCR was performed in a 96-well CFX Connect Bio-Rad RT-PCR Detection System using 10ng input cDNA; TaqMan Fast Advanced Master Mix (Applied Biosystems, 4444964); and stock TaqMan PCR primers from ThermoFisher Scientific (Tnfrsf19/TROY (Mm00443506_m1), LGR5 (Mm00438890_m1), and beta-actin/ACTB (Mm01205647_g1)).

Isolation:

Article Title: Multi-omics on truncating ASXL1 mutations in Bohring Opitz syndrome identify dysregulation of canonical and non-canonical Wnt signaling
Article Snippet: Peripheral blood samples were collected into EDTA tubes for DNA extraction and PBMC extraction, CPT tubes for PBMC extraction and PAXgeneTM Blood RNA Tube (BDBiosciences, 762165) for RNA extraction. .. Whole blood DNA was extracted using the Qiagen Blood and Cell Culture DNA Midi Kit (Qiagen #13343), and whole blood RNA was extracted using the MagMAXTM for Stabilized Blood Tubes RNA Isolation kit (Thermo Fisher Scientific, #4451893). .. All samples were quantified on the nanodrop, Qubit 4.0 and Tapestation Bioanalyzer for sample quality control.

Article Title: MiR-3201 as a Prognostic Blood Biomarker for Curative Treatments in Hepatocellular Carcinoma
Article Snippet: Peripheral blood (3 mL per patient) was collected in TempusTM Blood RNA Tubes containing 6 mL of Stabilizing Reagent (Thermo Fischer Scientific) and subsequently frozen at − 80 °C for long-term storage. .. Total RNA was isolated from TempusTM Blood RNA Tubes using the MagMAXTM for Stabilized Blood tubes RNA Isolation Kit (Thermo Fischer Scientific) following the manufacturer protocol. .. The quality of the total RNA extracted was assessed with the Agilent RNA 6000 Nano Kit (Agilent Technologies) using the 2100 Bioanalyzer Instrument (Agilent Technologies).

Article Title: Associations of four biological age markers with child development: A multi-omic analysis in the European HELIX cohort
Article Snippet: .. RNA was extracted from whole blood samples collected in Tempus tubes (Thermo Fisher Scientific, USA) using MagMAX for Stabilized Blood Tubes RNA Isolation Kit. .. The quality of RNA was evaluated with a 2100 Bioanalyzer (Agilent Technologies, USA) and the concentration with a NanoDrop 1000 UV-Vis Spectrophotometer.

Cell Culture:

Real-time Polymerase Chain Reaction:

Article Title: Critical Role of Toll-Like Receptor 9 in Morphine and Mycobacterium tuberculosis –Induced Apoptosis in Mice
Article Snippet: In situ apoptosis detection kit was purchased from Roche Diagnostic (Indianapolis, IN). .. The Quantitative PCR kit was purchased from Invitrogen (Carlsbad, CA). .. M. tuberculosis strain H37Ra was grown in 7H9 liquid medium (Difco) supplemented with 0.05% Tween 80 and 10% BSA-dextrose-catalase (ADC) enrichment (Difco) at 37°C for approximately 2 weeks with occasional agitation.

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