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superscript ii kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript ii kit
    Superscript Ii Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscripttm+iv+reverse+transcriptase+kit/Colorimetric+ReadiLAMP+Kit%2C+SARS-CoV-2/pmc03496065-323-6-9
    Average 94 stars, based on 15 article reviews
    superscript ii kit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: A Switch in the Control of Growth of the Wing Imaginal Disks of Manduca sexta
    Article Snippet: First stranded cDNA was generated using oligo-dT priming with the Superscript® II kit (Invitrogen).

    Article Title: Convergent Sets of Data from In Vivo and In Vitro Methods Point to an Active Role of Hsp60 in Chronic Obstructive Pulmonary Disease Pathogenesis
    Article Snippet: Intracellular proteins were quantified with the Quant-iTTM protein assay kit (Invitrogen Molecular Probes, Italy), using the Qubit fluorometer according to the manufacturer's instructions (the kit is accurate for protein concentrations ranging from 12.5 μg/ml to 5 mg/ml).

    Reverse Transcription:

    Article Title: Oxidized High-Density Lipoprotein Induces Endothelial Fibrosis Promoting Hyperpermeability, Hypotension, and Increased Mortality
    Article Snippet: Total RNA was extracted with Trizol according to the manufacturer’s protocol (Invitrogen, Carlsbad, CA, USA). .. DNAse I-treated RNA was used for reverse transcription using the Super Script II Kit (Invitrogen, USA). ..

    Article Title: Browning of White Adipose Tissue Uncouples Glucose Uptake from Insulin Signaling
    Article Snippet: RNA was isolated using the RNeasy Micro- and -Mini Kit (QIAGEN) for cells and tissues, respectively, performing on-column DNAse digest. .. 100 to 1000 ng RNA were reverse transcribed with the SuperScript II Kit (Invitrogen) and Oligo(dT) primers (Fermentas), then cDNA was diluted to a final concentration of 1 ng input RNA per μl cDNA and 5 μl were used for RT-PCR. .. Detection of gene expression was performed using the Taqman system with Taqman Gene Expression Master Mix and specific Gene Expression Assays and an ABI StepOnePlus sequence detector (Applied Biosystems). mRNA levels relative to TBP expression were calculated by the delta Ct method.

    Article Title: Evolution of T-cell clonality in a patient with Ph-negative acute lymphocytic leukemia occurring after interferon and imatinib therapy for Ph-positive chronic myeloid leukemia
    Article Snippet: RNA was extracted from the PBMC samples according to the manufacturer's recommendations (Trizol, Gibco, USA): The quality of RNA was analyzed in 0.8% agarose gel stained with ethidium bromide. .. Two μg RNA was reversely transcribed into the first single-strand cDNA with random hexamer primers, using reverse transcriptase, Superscript II Kit (Gibco, USA). ..

    Colorimetric Assay:

    Article Title: Cisplatin-induced oxidative stress stimulates renal Fas ligand shedding
    Article Snippet: .. Cisplatin-induced cytotoxicity was quantified using the LDH colorimetric assay kit (Life Technologies). ..

    Concentration Assay:

    Article Title: Browning of White Adipose Tissue Uncouples Glucose Uptake from Insulin Signaling
    Article Snippet: RNA was isolated using the RNeasy Micro- and -Mini Kit (QIAGEN) for cells and tissues, respectively, performing on-column DNAse digest. .. 100 to 1000 ng RNA were reverse transcribed with the SuperScript II Kit (Invitrogen) and Oligo(dT) primers (Fermentas), then cDNA was diluted to a final concentration of 1 ng input RNA per μl cDNA and 5 μl were used for RT-PCR. .. Detection of gene expression was performed using the Taqman system with Taqman Gene Expression Master Mix and specific Gene Expression Assays and an ABI StepOnePlus sequence detector (Applied Biosystems). mRNA levels relative to TBP expression were calculated by the delta Ct method.

    Random Hexamer:

    Article Title: Evolution of T-cell clonality in a patient with Ph-negative acute lymphocytic leukemia occurring after interferon and imatinib therapy for Ph-positive chronic myeloid leukemia
    Article Snippet: RNA was extracted from the PBMC samples according to the manufacturer's recommendations (Trizol, Gibco, USA): The quality of RNA was analyzed in 0.8% agarose gel stained with ethidium bromide. .. Two μg RNA was reversely transcribed into the first single-strand cDNA with random hexamer primers, using reverse transcriptase, Superscript II Kit (Gibco, USA). ..



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