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superscripttm iv reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscripttm iv reverse transcriptase kit
    Superscripttm Iv Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscripttm+iv+reverse+transcriptase+kit/high+capacity+cdna+reverse+transcription+kit/pm40265457-87-12-17
    Average 90 stars, based on 1 article reviews
    superscripttm iv reverse transcriptase kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Integrative machine learning reveals the biological function and prognostic significance of α-ketoglutarate in gastric cancer
    Article Snippet: RT-qPCR of each group was then performed using the PikoReal system (Thermo Fisher Scientific, Inc.) with a commercial kit (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. For transcriptomic analysis, polyadenylated mRNA was enriched using oligo(dT) magnetic beads, fragmented into short sequences and reverse transcribed into cDNA using the SuperScript III First-Strand Synthesis System (cat. no. 18080051; Invitrogen; Thermo Fisher Scientific, Inc.) with dNTPs (cat. no. R0192; Thermo Fisher Scientific, Inc.) and random hexamer primers. .. The reverse transcription temperature protocol was as follows: 2°C for 10 min, 50°C for 50 min and 70°C for 15 min. Double-stranded cDNA was synthesized, end-repaired, A-tailed and ligated to sequencing adapters using the NEBNext Ultra II DNA Library Prep Kit for Illumina (cat. no. E7645S; New England Biolabs, Inc.).

    Reverse Transcription:

    Article Title: Integrative machine learning reveals the biological function and prognostic significance of α-ketoglutarate in gastric cancer
    Article Snippet: RT-qPCR of each group was then performed using the PikoReal system (Thermo Fisher Scientific, Inc.) with a commercial kit (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. For transcriptomic analysis, polyadenylated mRNA was enriched using oligo(dT) magnetic beads, fragmented into short sequences and reverse transcribed into cDNA using the SuperScript III First-Strand Synthesis System (cat. no. 18080051; Invitrogen; Thermo Fisher Scientific, Inc.) with dNTPs (cat. no. R0192; Thermo Fisher Scientific, Inc.) and random hexamer primers. .. The reverse transcription temperature protocol was as follows: 2°C for 10 min, 50°C for 50 min and 70°C for 15 min. Double-stranded cDNA was synthesized, end-repaired, A-tailed and ligated to sequencing adapters using the NEBNext Ultra II DNA Library Prep Kit for Illumina (cat. no. E7645S; New England Biolabs, Inc.).

    Article Title: LncRNA levels in the central nervous system as novel potential players and biomarkers in amyotrophic lateral sclerosis
    Article Snippet: The quality and concentration of each sample were measured with Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific). .. RNA was retrotranscribed with the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific; Waltham, MA, USA). .. Quantitative PCR was performed from diluted cDNA in triplicates using the Quant Studio 3 Real Time PCR Instrument (Thermo Fisher Scientific).

    Random Hexamer:

    Article Title: Integrative machine learning reveals the biological function and prognostic significance of α-ketoglutarate in gastric cancer
    Article Snippet: RT-qPCR of each group was then performed using the PikoReal system (Thermo Fisher Scientific, Inc.) with a commercial kit (cat. no. RR820A; Takara Biotechnology Co., Ltd.). .. For transcriptomic analysis, polyadenylated mRNA was enriched using oligo(dT) magnetic beads, fragmented into short sequences and reverse transcribed into cDNA using the SuperScript III First-Strand Synthesis System (cat. no. 18080051; Invitrogen; Thermo Fisher Scientific, Inc.) with dNTPs (cat. no. R0192; Thermo Fisher Scientific, Inc.) and random hexamer primers. .. The reverse transcription temperature protocol was as follows: 2°C for 10 min, 50°C for 50 min and 70°C for 15 min. Double-stranded cDNA was synthesized, end-repaired, A-tailed and ligated to sequencing adapters using the NEBNext Ultra II DNA Library Prep Kit for Illumina (cat. no. E7645S; New England Biolabs, Inc.).

    other:

    Article Title: Lobetyolin alleviates IMQ-induced psoriasis-like skin inflammation by maintaining the homeostasis of the skin and inhibiting the inflammatory cytokines in dendritic cells
    Article Snippet: mRNA was purified from 2 μ g total RNA using mRNA Capture Beads 2.0 (cat. no. 12629ES; Shanghai Yeasen Biotechnology Co., Ltd.) through two rounds of poly(A) selection.

    Article Title: Reduced growth and biofilm formation at high temperatures contribute to Cryptococcus deneoformans dermatotropism
    Article Snippet: Microscopic examinations and measurements (μm) of cryptococcal biofilm-derived cell capsule and cell wall seeded on glass-bottom plates were performed by precisely tracing each structure using a Nikon Eclipse Ti2 inverted confocal laser scanning microscope and the NIS-Elements software.

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo.

    Article Title: Neonatal microbiota colonization primes maturation of goblet cell–mediated protection in the pre-weaning colon
    Article Snippet: Expression of Nox1 and Duox2 genes was analyzed by qRT-PCR of cDNA prepared from 600 ng RNA extractions using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific).

    Article Title: Antisense oligonucleotides targeting the SARS-CoV-2 nucleocapsid gene decrease viral titers in hamsters
    Article Snippet: HEK293T/ACE2 cells were co-transfected with the minigenome reporter plasmid and ASO candidates using Lipofectamine 2000 (Thermo Fisher Scientific) following manufacturer’s recommendations.

    Article Title: Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development
    Article Snippet: Briefly, 600 μL of TRIzol LS (Thermo Fischer Scientific) were added to 200 μL of culture medium; after 5 min of incubation at RT, 160 μL of chloroform were added.



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