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macs ms columns miltenyi biotec 130 042 201 superscriptii reverse transcriptase invitrogen
Macs Ms Columns Miltenyi Biotec 130 042 201 Superscriptii Reverse Transcriptase Invitrogen, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscriptiii+reverse+transcriptase/MS+Columns/pm29232557-203-38-41
Average 97 stars, based on 2460 article reviews
Macs Ms Columns Miltenyi Biotec 130 042 201 Superscriptii Reverse Transcriptase Invitrogen, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscriptiii+reverse+transcriptase/MS+Columns/pm29232557-203-38-41
Average 97 stars, based on 2460 article reviews
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2026-11
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Centrifugation:Article Title: Fibro-Adipogenic Progenitors From Skeletal Muscle Enhance Microvascular Angiogenesis in a 3D Model. Article Snippet: Briefly, the adherent ells were resuspended in 200 μL of DMEM + 2% FBS and ncubated with antibodies (1 μL per 2.0 × 106 cells) against DGFR α (ab203491, Abcam), SCA-1 (AB4336, Millipore Sigma), D31-PE (130-116-538, Miltenyi Biotec), and CD45-PE (130-126- 12, Miltenyi Biotec) on ice for 30 min. For CD31/CD45 depletion, ells were incubated with 10 μL of Anti-PE MicroBeads (130- 48-801, Miltenyi Biotec) on ice for 30 min, washed twice in mL of MACS buffer + 0.5% bovine serum albumin (BSA) via entrifugation (2000 rpm × 3 min at 4◦C), and subjected to negtive selection with LD Columns (130-042-901, Miltenyi Biotec) ccording to manufacturer protocols. .. For enrichment of PDGFR α nd SCA-1 cells, the flow-through from the previous step was ncubated with 10 μL of Anti-Rabbit IgG MicroBeads (130-048- 02, Miltenyi Biotec) on ice for 30 min, washed twice in 1 mL ACS buffer + 0.5% BSA via centrifugation (2000 rpm x 3 min at ◦C), and subjected to positive selection with MS Selection:Article Title: Fibro-Adipogenic Progenitors From Skeletal Muscle Enhance Microvascular Angiogenesis in a 3D Model. Article Snippet: Briefly, the adherent ells were resuspended in 200 μL of DMEM + 2% FBS and ncubated with antibodies (1 μL per 2.0 × 106 cells) against DGFR α (ab203491, Abcam), SCA-1 (AB4336, Millipore Sigma), D31-PE (130-116-538, Miltenyi Biotec), and CD45-PE (130-126- 12, Miltenyi Biotec) on ice for 30 min. For CD31/CD45 depletion, ells were incubated with 10 μL of Anti-PE MicroBeads (130- 48-801, Miltenyi Biotec) on ice for 30 min, washed twice in mL of MACS buffer + 0.5% bovine serum albumin (BSA) via entrifugation (2000 rpm × 3 min at 4◦C), and subjected to negtive selection with LD Columns (130-042-901, Miltenyi Biotec) ccording to manufacturer protocols. .. For enrichment of PDGFR α nd SCA-1 cells, the flow-through from the previous step was ncubated with 10 μL of Anti-Rabbit IgG MicroBeads (130-048- 02, Miltenyi Biotec) on ice for 30 min, washed twice in 1 mL ACS buffer + 0.5% BSA via centrifugation (2000 rpm x 3 min at ◦C), and subjected to positive selection with MS Mass Spectrometry:Article Title: Fibro-Adipogenic Progenitors From Skeletal Muscle Enhance Microvascular Angiogenesis in a 3D Model. Article Snippet: Briefly, the adherent ells were resuspended in 200 μL of DMEM + 2% FBS and ncubated with antibodies (1 μL per 2.0 × 106 cells) against DGFR α (ab203491, Abcam), SCA-1 (AB4336, Millipore Sigma), D31-PE (130-116-538, Miltenyi Biotec), and CD45-PE (130-126- 12, Miltenyi Biotec) on ice for 30 min. For CD31/CD45 depletion, ells were incubated with 10 μL of Anti-PE MicroBeads (130- 48-801, Miltenyi Biotec) on ice for 30 min, washed twice in mL of MACS buffer + 0.5% bovine serum albumin (BSA) via entrifugation (2000 rpm × 3 min at 4◦C), and subjected to negtive selection with LD Columns (130-042-901, Miltenyi Biotec) ccording to manufacturer protocols. .. For enrichment of PDGFR α nd SCA-1 cells, the flow-through from the previous step was ncubated with 10 μL of Anti-Rabbit IgG MicroBeads (130-048- 02, Miltenyi Biotec) on ice for 30 min, washed twice in 1 mL ACS buffer + 0.5% BSA via centrifugation (2000 rpm x 3 min at ◦C), and subjected to positive selection with MS Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells. Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 Article Title: Astrocyte maturation creates a stage-specific niche that instructs hippocampal neurogenesis Article Snippet: .. The pellet was resuspended in 500 μL buffer and applied to an Article Title: A PRMT5-ANGPTL4 axis in brain endothelial cells regulates cerebellar inhibitory synaptogenesis and motor coordination. Article Snippet: Brain vasculature is essential for central nervous system function.. Here, we found a cerebrovascular signaling axis in which the arginine methyltransferase PRMT5 in endothelial cells promoted proper inhibitory synaptogenesis and motor coordination in male mice.. Cerebrovascular endothelial cell–specific deletion of Prmt5 in mice led to excessive inhibitory synaptic input onto Purkinje cells and progressive motor deficits. Article Title: Postprandial hypertriglyceridemia activates microglia to promote satiety via an ASC dependent signaling Article Snippet: .. To obtain a higher purity, the eluate containing microglia was applied onto a MS column (#130-042-201, Miltenyi Biotec) placed into the OctoMACS TM Separator (#130-042-108, Miltenyi Biotec). .. Article Title: Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and cells. Article Snippet: Bacterial and virus strains Toscana Virus; strain 1812 Isolated from patient (Cusi et al4) N/A Toscana Virus; strain 1500590; lineage A Alexander et al.3 N/A Biological samples Ifnar1− /− mice skin, spleen, lymph node, brain and foot tissues, and blood serum (Keskek Turk et al.1) N/A Chemicals, peptides, and recombinant proteins Streptomycin/penicillin Gibco Cat#15140122 GlutaMAX Gibco Cat#35050061 Tryptose Phosphate Broth Gibco Cat#18050039 Hanks balanced saline solution Sigma-Aldrich Cat#H9394 Collagenase P Roche Cat#11 213 857 001 Dispase II Roche Cat#04942078001 DNase I Roche Cat#10104159001 PerfeCTa SYBR Green FastMix Quantabio Cat#95072-250 Fetal bovine serum Sigma Aldrich Cat#F9665-500ML RNAlater solution Sigma Aldrich Cat#R0901-500ML TRIzol Invitrogen Cat#15596026 2X MEM (Temin’s modification) Gibco Cat#11935046 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#21969-035 Critical commercial assays Tumor-Associated Fibroblast Isolation Kit, mouse Miltenyi Biotec Cat#130-116-474 QIAquick PCR Purification Kit Qiagen Cat#28106 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 PureLink RNA Mini Extraction Kit Invitrogen Cat#12183018A PureLink RNA Micro Scale Kit Invitrogen Cat#12183016 Cytofix/Cytoperm Fixation/Permeabilization Kit BD Cat#554714 Zymo Quick-RNA Microprep Kit Zymo Research Cat#R1050 Experimental models: Cell lines Baby Hamster Kidney-21 Derived from Lefteri et al.5 RRID: CVCL_1914 Vero cells Derived from Lefteri et al.5 RRID: CVCL_0059 Experimental models: Organisms/strains B6(Cg)-Ifnar1tm1.2Ees/J mice Strain #:028288 (Mus musculus; Ifnar1− /− , homozygous; male and female; 6–10 weeks of age) Jackson Laboratory RRID: IMSR_JAX:028288 Phlebotomus perniciosus (Murcia, Spain; laboratory colony) P. Volf, Charles University, Czech Republic N/A Lutzomyia longipalpis (laboratory colony) M.E. .. Rogers, LSHTM, UK N/A Oligonucleotides See Table 1 for all sequences N/A N/A Software and algorithms CytExpert software; CytoFLEX Platform Beckman Coulter N/A GraphPad Prism GraphPad Software RRID: SCR_002798 Other Article Title: Tissue Dissociation Workflows Influence Reactive Astrocyte Recovery in Single-Cell Transcriptomics Article Snippet: Labeled cells were washed with 1 mL of AstroMACS Separation Buffer (Miltenyi Biotec, Cat#130-117-336) with a wide-bore P1000 tip and centrifuged (300 × g , 5 min, 4 °C, break 3, acceleration 3). .. The pellet was resuspended in 500 μL of AstroMACS Separation Buffer with a standard P1000 tip and loaded onto a Isolation:Article Title: Sex-Stratified Characterization of Behavioral, Hippocampal, and Microglial Androgen Responses to Adolescent High-Fat Diet Consumption in Mice. Article Snippet: After the removal of cell debris using a High Efficiency Debris Removal Kit (DHDR-5006, RWD), the cells were resuspended in magnetic activated cell sorting (MACS) buffer (PBS with 2 mM EDTA and 0.5% BSA) and then incubated with anti-CD11b microbeads (130-126-725, Miltenyi Biotec, Bergisch Gladbach, Germany). .. Magnetic Cell Separation:Article Title: Sex-Stratified Characterization of Behavioral, Hippocampal, and Microglial Androgen Responses to Adolescent High-Fat Diet Consumption in Mice. Article Snippet: After the removal of cell debris using a High Efficiency Debris Removal Kit (DHDR-5006, RWD), the cells were resuspended in magnetic activated cell sorting (MACS) buffer (PBS with 2 mM EDTA and 0.5% BSA) and then incubated with anti-CD11b microbeads (130-126-725, Miltenyi Biotec, Bergisch Gladbach, Germany). .. Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells. Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 Recombinant:Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells. Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 Cell Isolation:Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells. Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 Software:Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells. Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 Article Title: Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and cells. Article Snippet: Bacterial and virus strains Toscana Virus; strain 1812 Isolated from patient (Cusi et al4) N/A Toscana Virus; strain 1500590; lineage A Alexander et al.3 N/A Biological samples Ifnar1− /− mice skin, spleen, lymph node, brain and foot tissues, and blood serum (Keskek Turk et al.1) N/A Chemicals, peptides, and recombinant proteins Streptomycin/penicillin Gibco Cat#15140122 GlutaMAX Gibco Cat#35050061 Tryptose Phosphate Broth Gibco Cat#18050039 Hanks balanced saline solution Sigma-Aldrich Cat#H9394 Collagenase P Roche Cat#11 213 857 001 Dispase II Roche Cat#04942078001 DNase I Roche Cat#10104159001 PerfeCTa SYBR Green FastMix Quantabio Cat#95072-250 Fetal bovine serum Sigma Aldrich Cat#F9665-500ML RNAlater solution Sigma Aldrich Cat#R0901-500ML TRIzol Invitrogen Cat#15596026 2X MEM (Temin’s modification) Gibco Cat#11935046 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#21969-035 Critical commercial assays Tumor-Associated Fibroblast Isolation Kit, mouse Miltenyi Biotec Cat#130-116-474 QIAquick PCR Purification Kit Qiagen Cat#28106 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 PureLink RNA Mini Extraction Kit Invitrogen Cat#12183018A PureLink RNA Micro Scale Kit Invitrogen Cat#12183016 Cytofix/Cytoperm Fixation/Permeabilization Kit BD Cat#554714 Zymo Quick-RNA Microprep Kit Zymo Research Cat#R1050 Experimental models: Cell lines Baby Hamster Kidney-21 Derived from Lefteri et al.5 RRID: CVCL_1914 Vero cells Derived from Lefteri et al.5 RRID: CVCL_0059 Experimental models: Organisms/strains B6(Cg)-Ifnar1tm1.2Ees/J mice Strain #:028288 (Mus musculus; Ifnar1− /− , homozygous; male and female; 6–10 weeks of age) Jackson Laboratory RRID: IMSR_JAX:028288 Phlebotomus perniciosus (Murcia, Spain; laboratory colony) P. Volf, Charles University, Czech Republic N/A Lutzomyia longipalpis (laboratory colony) M.E. .. Rogers, LSHTM, UK N/A Oligonucleotides See Table 1 for all sequences N/A N/A Software and algorithms CytExpert software; CytoFLEX Platform Beckman Coulter N/A GraphPad Prism GraphPad Software RRID: SCR_002798 Other Cell Culture:Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells. Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 Labeling:Article Title: A PRMT5-ANGPTL4 axis in brain endothelial cells regulates cerebellar inhibitory synaptogenesis and motor coordination. Article Snippet: Brain vasculature is essential for central nervous system function.. Here, we found a cerebrovascular signaling axis in which the arginine methyltransferase PRMT5 in endothelial cells promoted proper inhibitory synaptogenesis and motor coordination in male mice.. Cerebrovascular endothelial cell–specific deletion of Prmt5 in mice led to excessive inhibitory synaptic input onto Purkinje cells and progressive motor deficits. Control:Article Title: Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and cells. Article Snippet: Bacterial and virus strains Toscana Virus; strain 1812 Isolated from patient (Cusi et al4) N/A Toscana Virus; strain 1500590; lineage A Alexander et al.3 N/A Biological samples Ifnar1− /− mice skin, spleen, lymph node, brain and foot tissues, and blood serum (Keskek Turk et al.1) N/A Chemicals, peptides, and recombinant proteins Streptomycin/penicillin Gibco Cat#15140122 GlutaMAX Gibco Cat#35050061 Tryptose Phosphate Broth Gibco Cat#18050039 Hanks balanced saline solution Sigma-Aldrich Cat#H9394 Collagenase P Roche Cat#11 213 857 001 Dispase II Roche Cat#04942078001 DNase I Roche Cat#10104159001 PerfeCTa SYBR Green FastMix Quantabio Cat#95072-250 Fetal bovine serum Sigma Aldrich Cat#F9665-500ML RNAlater solution Sigma Aldrich Cat#R0901-500ML TRIzol Invitrogen Cat#15596026 2X MEM (Temin’s modification) Gibco Cat#11935046 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#21969-035 Critical commercial assays Tumor-Associated Fibroblast Isolation Kit, mouse Miltenyi Biotec Cat#130-116-474 QIAquick PCR Purification Kit Qiagen Cat#28106 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 PureLink RNA Mini Extraction Kit Invitrogen Cat#12183018A PureLink RNA Micro Scale Kit Invitrogen Cat#12183016 Cytofix/Cytoperm Fixation/Permeabilization Kit BD Cat#554714 Zymo Quick-RNA Microprep Kit Zymo Research Cat#R1050 Experimental models: Cell lines Baby Hamster Kidney-21 Derived from Lefteri et al.5 RRID: CVCL_1914 Vero cells Derived from Lefteri et al.5 RRID: CVCL_0059 Experimental models: Organisms/strains B6(Cg)-Ifnar1tm1.2Ees/J mice Strain #:028288 (Mus musculus; Ifnar1− /− , homozygous; male and female; 6–10 weeks of age) Jackson Laboratory RRID: IMSR_JAX:028288 Phlebotomus perniciosus (Murcia, Spain; laboratory colony) P. Volf, Charles University, Czech Republic N/A Lutzomyia longipalpis (laboratory colony) M.E. .. Rogers, LSHTM, UK N/A Oligonucleotides See Table 1 for all sequences N/A N/A Software and algorithms CytExpert software; CytoFLEX Platform Beckman Coulter N/A GraphPad Prism GraphPad Software RRID: SCR_002798 Other Blocking Assay:Article Title: Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and cells. Article Snippet: Bacterial and virus strains Toscana Virus; strain 1812 Isolated from patient (Cusi et al4) N/A Toscana Virus; strain 1500590; lineage A Alexander et al.3 N/A Biological samples Ifnar1− /− mice skin, spleen, lymph node, brain and foot tissues, and blood serum (Keskek Turk et al.1) N/A Chemicals, peptides, and recombinant proteins Streptomycin/penicillin Gibco Cat#15140122 GlutaMAX Gibco Cat#35050061 Tryptose Phosphate Broth Gibco Cat#18050039 Hanks balanced saline solution Sigma-Aldrich Cat#H9394 Collagenase P Roche Cat#11 213 857 001 Dispase II Roche Cat#04942078001 DNase I Roche Cat#10104159001 PerfeCTa SYBR Green FastMix Quantabio Cat#95072-250 Fetal bovine serum Sigma Aldrich Cat#F9665-500ML RNAlater solution Sigma Aldrich Cat#R0901-500ML TRIzol Invitrogen Cat#15596026 2X MEM (Temin’s modification) Gibco Cat#11935046 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#21969-035 Critical commercial assays Tumor-Associated Fibroblast Isolation Kit, mouse Miltenyi Biotec Cat#130-116-474 QIAquick PCR Purification Kit Qiagen Cat#28106 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 PureLink RNA Mini Extraction Kit Invitrogen Cat#12183018A PureLink RNA Micro Scale Kit Invitrogen Cat#12183016 Cytofix/Cytoperm Fixation/Permeabilization Kit BD Cat#554714 Zymo Quick-RNA Microprep Kit Zymo Research Cat#R1050 Experimental models: Cell lines Baby Hamster Kidney-21 Derived from Lefteri et al.5 RRID: CVCL_1914 Vero cells Derived from Lefteri et al.5 RRID: CVCL_0059 Experimental models: Organisms/strains B6(Cg)-Ifnar1tm1.2Ees/J mice Strain #:028288 (Mus musculus; Ifnar1− /− , homozygous; male and female; 6–10 weeks of age) Jackson Laboratory RRID: IMSR_JAX:028288 Phlebotomus perniciosus (Murcia, Spain; laboratory colony) P. Volf, Charles University, Czech Republic N/A Lutzomyia longipalpis (laboratory colony) M.E. .. Rogers, LSHTM, UK N/A Oligonucleotides See Table 1 for all sequences N/A N/A Software and algorithms CytExpert software; CytoFLEX Platform Beckman Coulter N/A GraphPad Prism GraphPad Software RRID: SCR_002798 Other |