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Enzynomics co Ltd superscript iii reverse transcriptase
Superscript Iii Reverse Transcriptase, supplied by Enzynomics co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+reverse+transcriptase/superscript+iii+reverse+transcriptase/pm39478550-117-17-21
Average 90 stars, based on 1 article reviews
superscript iii reverse transcriptase - by Bioz Stars, 2026-09
90/100 stars

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Synthesized:

Article Title: Tunicamycins from Marine-Derived Streptomyces bacillaris Inhibit MurNAc-Pentapeptide Translocase in Staphylococcus aureus
Article Snippet: Lysostaphin was employed to enzymatically degrade the cell wall of S. aureus , followed by total RNA extraction using the easy-BLUETM reagent (Intron Biotechnology, Seoul, Republic of Korea). .. Subsequently, cDNA was synthesized using the SuperScript III cDNA synthesis kit (Enzynomics, Seoul, Republic of Korea), and semi-quantitative RT–PCR was conducted with mraY -specific primers ( mraY For and mraY Rev) ( ) under standard conditions. ..

Article Title: Transcriptome profiling reveals novel insights into the regulation of calcium ion and detoxification genes driving chlorantraniliprole resistance in Spodoptera exigua
Article Snippet: .. To validate the expression of differentially expressed candidate genes, complementary DNA (cDNA) was synthesized from the total RNA extracted from susceptible, resistant, and field populations using a RNeasy Plus Mini Kit (Qiagen) and Superscript III cDNA Synthesis Kit (Enzynomics, Daejeon, Korea). .. Quantitative real-time PCR (qPCR) was performed using specific primers for each target candidate gene and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as the reference gene ( ). qPCR was conducted using the ToprealTM SYBR Green qPCR PreMIX (Enzynomics) on the ABI QuantStudio 1 (Thermo Fisher Scientific Inc., Waltham, MA, USA) platform. qPCR was performed in triplicate in a total volume of 20 μL, following the protocol provided by the manufacturer.

Article Title: A bacterial site 2 protease contributes to RNA phage resistance by targeting phage lysis protein
Article Snippet: .. Phages were isolated from the plaques and RNA was extracted using TRIzol (Invitrogen). cDNA was synthesized from 10 ng of RNA using SuperScript III reverse transcriptase (Enzynomics) with PP7-R primer. ..

Article Title: Tunicamycins from Marine-Derived Streptomyces bacillaris Inhibit MurNAc-Pentapeptide Translocase in Staphylococcus aureus .
Article Snippet: Lysostaphin was employed to enzymatically degrade the cell wall of S. aureus, followed by total RNA extraction using the easy-BLUETM reagent (Intron Biotechnology, Seoul, Republic of Korea). .. Subsequently, cDNA was synthesized using the SuperScript III cDNA synthesis kit (Enzynomics, Seoul, Republic of Korea), and semiquantitative RT–PCR was conducted with mraY-specific primers (mraY For and mraY Rev) (Table S1) under standard conditions. ..

cDNA Synthesis:

Article Title: Tunicamycins from Marine-Derived Streptomyces bacillaris Inhibit MurNAc-Pentapeptide Translocase in Staphylococcus aureus
Article Snippet: Lysostaphin was employed to enzymatically degrade the cell wall of S. aureus , followed by total RNA extraction using the easy-BLUETM reagent (Intron Biotechnology, Seoul, Republic of Korea). .. Subsequently, cDNA was synthesized using the SuperScript III cDNA synthesis kit (Enzynomics, Seoul, Republic of Korea), and semi-quantitative RT–PCR was conducted with mraY -specific primers ( mraY For and mraY Rev) ( ) under standard conditions. ..

Article Title: Transcriptome profiling reveals novel insights into the regulation of calcium ion and detoxification genes driving chlorantraniliprole resistance in Spodoptera exigua
Article Snippet: .. To validate the expression of differentially expressed candidate genes, complementary DNA (cDNA) was synthesized from the total RNA extracted from susceptible, resistant, and field populations using a RNeasy Plus Mini Kit (Qiagen) and Superscript III cDNA Synthesis Kit (Enzynomics, Daejeon, Korea). .. Quantitative real-time PCR (qPCR) was performed using specific primers for each target candidate gene and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as the reference gene ( ). qPCR was conducted using the ToprealTM SYBR Green qPCR PreMIX (Enzynomics) on the ABI QuantStudio 1 (Thermo Fisher Scientific Inc., Waltham, MA, USA) platform. qPCR was performed in triplicate in a total volume of 20 μL, following the protocol provided by the manufacturer.

Article Title: Identification of a novel locus qGW12/OsPUB23 regulating grain shape and weight in rice (Oryza sativa L.).
Article Snippet: Key message Key message A major quantitative trait locus (qGW12) for grain shape and weight has been isolated in rice, corresponding to LOC_Os12g17900/OsPUB23, and its encoded protein interacts with OsMADS1.. Abstract Grain shape in rice is an important trait that influences both yield and quality.. The primary determinants of grain shape are quantitative trait loci (QTLs) inherited from natural variation in crops.

Article Title: Tunicamycins from Marine-Derived Streptomyces bacillaris Inhibit MurNAc-Pentapeptide Translocase in Staphylococcus aureus .
Article Snippet: Lysostaphin was employed to enzymatically degrade the cell wall of S. aureus, followed by total RNA extraction using the easy-BLUETM reagent (Intron Biotechnology, Seoul, Republic of Korea). .. Subsequently, cDNA was synthesized using the SuperScript III cDNA synthesis kit (Enzynomics, Seoul, Republic of Korea), and semiquantitative RT–PCR was conducted with mraY-specific primers (mraY For and mraY Rev) (Table S1) under standard conditions. ..

Expressing:

Article Title: Transcriptome profiling reveals novel insights into the regulation of calcium ion and detoxification genes driving chlorantraniliprole resistance in Spodoptera exigua
Article Snippet: .. To validate the expression of differentially expressed candidate genes, complementary DNA (cDNA) was synthesized from the total RNA extracted from susceptible, resistant, and field populations using a RNeasy Plus Mini Kit (Qiagen) and Superscript III cDNA Synthesis Kit (Enzynomics, Daejeon, Korea). .. Quantitative real-time PCR (qPCR) was performed using specific primers for each target candidate gene and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as the reference gene ( ). qPCR was conducted using the ToprealTM SYBR Green qPCR PreMIX (Enzynomics) on the ABI QuantStudio 1 (Thermo Fisher Scientific Inc., Waltham, MA, USA) platform. qPCR was performed in triplicate in a total volume of 20 μL, following the protocol provided by the manufacturer.

Isolation:

Article Title: A bacterial site 2 protease contributes to RNA phage resistance by targeting phage lysis protein
Article Snippet: .. Phages were isolated from the plaques and RNA was extracted using TRIzol (Invitrogen). cDNA was synthesized from 10 ng of RNA using SuperScript III reverse transcriptase (Enzynomics) with PP7-R primer. ..

Reverse Transcription:

Article Title: A bacterial site 2 protease contributes to RNA phage resistance by targeting phage lysis protein
Article Snippet: .. Phages were isolated from the plaques and RNA was extracted using TRIzol (Invitrogen). cDNA was synthesized from 10 ng of RNA using SuperScript III reverse transcriptase (Enzynomics) with PP7-R primer. ..

Article Title: ELF18-INDUCED LONG NONCODING RNA 11 negatively regulates the innate immune response by suppressing Pathogenesis-related Lipase 5.
Article Snippet: Long noncoding RNAs (lncRNAs) are essential components of diverse biological processes.. However, lncRNAs involved in the innate immune response remain poorly unknown in plants.. Here, we isolated ten lncRNAs named ELF18-INDUCED LONG NONCODING RNA (ELENA2 to 11) from custom lncRNA array datasets generated from Arabidopsis seedlings treated with pathogen-associated molecular patterns (PAMPs; elf18 and flg22).

Article Title: Identification of a novel locus qGW12/OsPUB23 regulating grain shape and weight in rice (Oryza sativa L.).
Article Snippet: Key message Key message A major quantitative trait locus (qGW12) for grain shape and weight has been isolated in rice, corresponding to LOC_Os12g17900/OsPUB23, and its encoded protein interacts with OsMADS1.. Abstract Grain shape in rice is an important trait that influences both yield and quality.. The primary determinants of grain shape are quantitative trait loci (QTLs) inherited from natural variation in crops.

Article Title: A non-catalytic role of IPMK is required for PLCγ1 activation in T cell receptor signaling by stabilizing the PLCγ1-Sam68 complex.
Article Snippet: .. A total of 1 μg of total RNA was used for the synthesis of firststrand cDNA by Superscript III reverse transcriptase (enzynomics). .. Quantitative real-time PCR analysis was performed using the SYBR Green master mix (Toyobo) and the Step One Plus Real-Time PCR system (Applied Biosystems).

Article Title: A non-catalytic role of IPMK is required for PLCγ1 activation in T cell receptor signaling by stabilizing the PLCγ1-Sam68 complex
Article Snippet: .. A total of 1 μg of total RNA was used for the synthesis of first-strand cDNA by Superscript III reverse transcriptase (enzynomics). .. Quantitative real-time PCR analysis was performed using the SYBR Green master mix (Toyobo) and the Step One Plus Real-Time PCR system (Applied Biosystems).



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