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TransGen biotech co superscript ii reverse transcriptase
Superscript Ii Reverse Transcriptase, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iv+reverse+transcriptase/superscript+ii+reverse+transcriptase/pm36693541-298-16-20
Average 90 stars, based on 1 article reviews
superscript ii reverse transcriptase - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Incubation:

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling.
Article Snippet: Afterwards, si-control and siRNAs were transfected into SMMC-7721 cells; pc-SCML2 and vector were transfected into Hep3B cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). .. After incubation for 48 h, the transfection efficiency was confirmed by qRT-PCR. qRT-PCR Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Transfection:

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling.
Article Snippet: Afterwards, si-control and siRNAs were transfected into SMMC-7721 cells; pc-SCML2 and vector were transfected into Hep3B cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). .. After incubation for 48 h, the transfection efficiency was confirmed by qRT-PCR. qRT-PCR Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Quantitative RT-PCR:

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling.
Article Snippet: Afterwards, si-control and siRNAs were transfected into SMMC-7721 cells; pc-SCML2 and vector were transfected into Hep3B cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). .. After incubation for 48 h, the transfection efficiency was confirmed by qRT-PCR. qRT-PCR Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Synthesized:

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling.
Article Snippet: Afterwards, si-control and siRNAs were transfected into SMMC-7721 cells; pc-SCML2 and vector were transfected into Hep3B cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). .. After incubation for 48 h, the transfection efficiency was confirmed by qRT-PCR. qRT-PCR Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Article Title: Natural variation and CRISPR/Cas9‐mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean
Article Snippet: .. For reverse transcription and quantitative real‐time PCR, total RNA was isolated from different tissues using the Trizol Up Plus RNA Kit (Tiangen Biotech). cDNAs were synthesized with Superscript II reverse transcriptase (TransGen Biotech). .. The qRT‐PCR was performed using the ABI7900 system (Applied Biosystems).

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling
Article Snippet: .. Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Article Title: Cotyledons facilitate the adaptation of early‐maturing soybean varieties to high‐latitude long‐day environments
Article Snippet: .. mRNA was extracted using a TransZol Up kit (TransGen Biotech, Beijing, China) and cDNA was synthesized with Superscript II reverse transcriptase (TransGen Biotech). .. We performed qPCR using an ABI7500 Thermocycler (Applied Biosystems, Foster City, CA) with the KAPA SYBR DNA Polymerase (KAPA Biosystems, Cape Town, South Africa).

Reverse Transcription:

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling.
Article Snippet: Afterwards, si-control and siRNAs were transfected into SMMC-7721 cells; pc-SCML2 and vector were transfected into Hep3B cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). .. After incubation for 48 h, the transfection efficiency was confirmed by qRT-PCR. qRT-PCR Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Article Title: Evolution of gene regulatory network of C 4 photosynthesis in the genus Flaveria reveals the evolutionary status of C 3 -C 4 intermediate species.
Article Snippet: .. Between 0.2 and 0.5 mg RNA was used for reverse transcription to synthesize first-strand cDNA with Superscript II Reverse Transcriptase (TransGen Biotech, Beijing). .. The qRT–PCR mixture was prepared following the instructions of Unicon qPCR SYBR Green Master Mix (Yeasen, China).

Article Title: HuR confers IL-17a-induced migration and invasion of gastric cancer cells via upregulation of Snail translation.
Article Snippet: Human gastric cancer is a leading cause of cancer mortality in the world wide.. We found that the expression of IL-17a was significantly increased in gastric cancer cells.. Treatment with recombinant IL-17a (rIL-17a) can increase migration, invasion and epithelial to mesenchymal transition (EMT) of gastric cancer cells.

Article Title: Natural variation and CRISPR/Cas9‐mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean
Article Snippet: .. For reverse transcription and quantitative real‐time PCR, total RNA was isolated from different tissues using the Trizol Up Plus RNA Kit (Tiangen Biotech). cDNAs were synthesized with Superscript II reverse transcriptase (TransGen Biotech). .. The qRT‐PCR was performed using the ABI7900 system (Applied Biosystems).

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling
Article Snippet: .. Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Article Title: Cotyledons facilitate the adaptation of early‐maturing soybean varieties to high‐latitude long‐day environments
Article Snippet: .. mRNA was extracted using a TransZol Up kit (TransGen Biotech, Beijing, China) and cDNA was synthesized with Superscript II reverse transcriptase (TransGen Biotech). .. We performed qPCR using an ABI7500 Thermocycler (Applied Biosystems, Foster City, CA) with the KAPA SYBR DNA Polymerase (KAPA Biosystems, Cape Town, South Africa).

Article Title: Evolution of gene regulatory network of C 4 photosynthesis in the genus Flaveria reveals the evolutionary status of C 3 -C 4 intermediate species
Article Snippet: .. Between 0.2 and 0.5 μg RNA was used for reverse transcription to synthesize first-strand cDNA with Superscript II Reverse Transcriptase (TransGen Biotech, Beijing). .. The qRT–PCR mixture was prepared following the instructions of Unicon qPCR SYBR Green Master Mix (Yeasen, China).

Real-time Polymerase Chain Reaction:

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling.
Article Snippet: Afterwards, si-control and siRNAs were transfected into SMMC-7721 cells; pc-SCML2 and vector were transfected into Hep3B cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). .. After incubation for 48 h, the transfection efficiency was confirmed by qRT-PCR. qRT-PCR Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Article Title: Natural variation and CRISPR/Cas9‐mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean
Article Snippet: .. For reverse transcription and quantitative real‐time PCR, total RNA was isolated from different tissues using the Trizol Up Plus RNA Kit (Tiangen Biotech). cDNAs were synthesized with Superscript II reverse transcriptase (TransGen Biotech). .. The qRT‐PCR was performed using the ABI7900 system (Applied Biosystems).

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling
Article Snippet: .. Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

SYBR Green Assay:

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling.
Article Snippet: Afterwards, si-control and siRNAs were transfected into SMMC-7721 cells; pc-SCML2 and vector were transfected into Hep3B cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). .. After incubation for 48 h, the transfection efficiency was confirmed by qRT-PCR. qRT-PCR Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Article Title: Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling
Article Snippet: .. Total RNA in tumor tissues and cells was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized by a SuperScript III reverse transcriptase (TransGen Biotech, Beijing, China). qPCR was performed using the ABI Prism 7500 system (Applied Biosystems, Carlsbad, CA, USA) with SYBR Green qPCR Master Mix (Toyobo, Osaka, Japan). ..

Isolation:

Article Title: Natural variation and CRISPR/Cas9‐mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean
Article Snippet: .. For reverse transcription and quantitative real‐time PCR, total RNA was isolated from different tissues using the Trizol Up Plus RNA Kit (Tiangen Biotech). cDNAs were synthesized with Superscript II reverse transcriptase (TransGen Biotech). .. The qRT‐PCR was performed using the ABI7900 system (Applied Biosystems).



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