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Epicentre Biotechnologies superscript iii reverse transcriptase
Superscript Iii Reverse Transcriptase, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+transcriptase/superscript+iii+reverse+transcriptase/pmc07047781-82-38-42
Average 90 stars, based on 1 article reviews
superscript iii reverse transcriptase - by Bioz Stars, 2026-11
90/100 stars

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Reverse Transcription:

Article Title: MicroRNA-181c provides neuroprotection in an intracerebral hemorrhage model
Article Snippet: .. Total RNA was detected by adding 750 μL of TRIzol reagent (Molecular Research Center, Cincinnati, OH, USA) and 20 μL of glacial acetic acid to 200 μL of blood sample; 800 ng of RNA was reverse transcribed using SuperScript III Reverse Transcriptase (Epicentre Biotechnologies, Madison, WI, USA) with miRNA RT primer (Bioligo, Shanghai, China). .. Total RNA was detected by adding 750 μL of TRIzol reagent (Molecular Research Center, Cincinnati, OH, USA) and 20 μL of glacial acetic acid to 200 μL of blood sample; 800 ng of RNA was reverse transcribed using SuperScript III Reverse Transcriptase (Epicentre Biotechnologies, Madison, WI, USA) with miRNA RT primer (Bioligo, Shanghai, China).

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: Total RNA samples were isolated from whole epididymal adipose tissue using TRIzol Reagent from Invitrogen (Carlsbad, CA, USA) and quantified by spectrometry. .. First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers. .. Messenger RNA levels of ATP-binding cassette A1 (ABCA1), ABCG1, ApoE, low-density lipoprotein receptor, perilipin-1 (PLIN1), PLIN2, adipose tissue lipase, HSL, PPARalpha, PGC-1alpha, CPT1, UCP2 and L32 were measured by real-time reverse transcriptase-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA, USA) and SYBR Green Master Mix (Bioline USA Inc., Tauton, MA, USA).

Article Title: ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction
Article Snippet: Molecular band intensity was determined by densitometry using NIH ImageJ software. .. Real-time quantitative RT-PCR: Total RNA was extracted from adipose tissue or cells using TRIzol Reagent (Invitrogen, Carlsbad, CA). cDNA was synthesized using Superscript III reverse transcriptase (Epicentre Biotechnologies, Madison, WI) and random hexamer primers. .. Messenger RNA levels of mouse Nos2 (nitric oxide synthase 2) (50), Chil3 (chitinase-like 3), Il6 (interleukin 6), Arg1 (arginase), Abca1 (ABC binding cassette A1), Abcg1, Acat (acetyl-coenzyme A acetyltransferase 1), MCP1 (monocyte chemoattractant protein 1) and L32 were measured by real-time RT-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA) and SybrGreen Master Mix (Bioline USA Inc., by guest, on O ctober 27, 2015 w w w .jlr.org D ow nloaded from method as previously described (51).

miRNA RT:

Article Title: MicroRNA-181c provides neuroprotection in an intracerebral hemorrhage model
Article Snippet: .. Total RNA was detected by adding 750 μL of TRIzol reagent (Molecular Research Center, Cincinnati, OH, USA) and 20 μL of glacial acetic acid to 200 μL of blood sample; 800 ng of RNA was reverse transcribed using SuperScript III Reverse Transcriptase (Epicentre Biotechnologies, Madison, WI, USA) with miRNA RT primer (Bioligo, Shanghai, China). .. Total RNA was detected by adding 750 μL of TRIzol reagent (Molecular Research Center, Cincinnati, OH, USA) and 20 μL of glacial acetic acid to 200 μL of blood sample; 800 ng of RNA was reverse transcribed using SuperScript III Reverse Transcriptase (Epicentre Biotechnologies, Madison, WI, USA) with miRNA RT primer (Bioligo, Shanghai, China).

Synthesized:

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: Total RNA samples were isolated from whole epididymal adipose tissue using TRIzol Reagent from Invitrogen (Carlsbad, CA, USA) and quantified by spectrometry. .. First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers. .. Messenger RNA levels of ATP-binding cassette A1 (ABCA1), ABCG1, ApoE, low-density lipoprotein receptor, perilipin-1 (PLIN1), PLIN2, adipose tissue lipase, HSL, PPARalpha, PGC-1alpha, CPT1, UCP2 and L32 were measured by real-time reverse transcriptase-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA, USA) and SYBR Green Master Mix (Bioline USA Inc., Tauton, MA, USA).

Article Title: ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction
Article Snippet: Molecular band intensity was determined by densitometry using NIH ImageJ software. .. Real-time quantitative RT-PCR: Total RNA was extracted from adipose tissue or cells using TRIzol Reagent (Invitrogen, Carlsbad, CA). cDNA was synthesized using Superscript III reverse transcriptase (Epicentre Biotechnologies, Madison, WI) and random hexamer primers. .. Messenger RNA levels of mouse Nos2 (nitric oxide synthase 2) (50), Chil3 (chitinase-like 3), Il6 (interleukin 6), Arg1 (arginase), Abca1 (ABC binding cassette A1), Abcg1, Acat (acetyl-coenzyme A acetyltransferase 1), MCP1 (monocyte chemoattractant protein 1) and L32 were measured by real-time RT-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA) and SybrGreen Master Mix (Bioline USA Inc., by guest, on O ctober 27, 2015 w w w .jlr.org D ow nloaded from method as previously described (51).

Random Hexamer:

Article Title: Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice
Article Snippet: Total RNA samples were isolated from whole epididymal adipose tissue using TRIzol Reagent from Invitrogen (Carlsbad, CA, USA) and quantified by spectrometry. .. First-strand complementary DNAs were synthesized using Superscript III reverse transcriptase (EPICENTRE Biotechnologies, Madison, WI, USA) and random hexamer primers. .. Messenger RNA levels of ATP-binding cassette A1 (ABCA1), ABCG1, ApoE, low-density lipoprotein receptor, perilipin-1 (PLIN1), PLIN2, adipose tissue lipase, HSL, PPARalpha, PGC-1alpha, CPT1, UCP2 and L32 were measured by real-time reverse transcriptase-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA, USA) and SYBR Green Master Mix (Bioline USA Inc., Tauton, MA, USA).

Article Title: ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction
Article Snippet: Molecular band intensity was determined by densitometry using NIH ImageJ software. .. Real-time quantitative RT-PCR: Total RNA was extracted from adipose tissue or cells using TRIzol Reagent (Invitrogen, Carlsbad, CA). cDNA was synthesized using Superscript III reverse transcriptase (Epicentre Biotechnologies, Madison, WI) and random hexamer primers. .. Messenger RNA levels of mouse Nos2 (nitric oxide synthase 2) (50), Chil3 (chitinase-like 3), Il6 (interleukin 6), Arg1 (arginase), Abca1 (ABC binding cassette A1), Abcg1, Acat (acetyl-coenzyme A acetyltransferase 1), MCP1 (monocyte chemoattractant protein 1) and L32 were measured by real-time RT-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA) and SybrGreen Master Mix (Bioline USA Inc., by guest, on O ctober 27, 2015 w w w .jlr.org D ow nloaded from method as previously described (51).

Quantitative RT-PCR:

Article Title: ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction
Article Snippet: Molecular band intensity was determined by densitometry using NIH ImageJ software. .. Real-time quantitative RT-PCR: Total RNA was extracted from adipose tissue or cells using TRIzol Reagent (Invitrogen, Carlsbad, CA). cDNA was synthesized using Superscript III reverse transcriptase (Epicentre Biotechnologies, Madison, WI) and random hexamer primers. .. Messenger RNA levels of mouse Nos2 (nitric oxide synthase 2) (50), Chil3 (chitinase-like 3), Il6 (interleukin 6), Arg1 (arginase), Abca1 (ABC binding cassette A1), Abcg1, Acat (acetyl-coenzyme A acetyltransferase 1), MCP1 (monocyte chemoattractant protein 1) and L32 were measured by real-time RT-PCR using 7500 real-time PCR System (Applied Biosystems, Foster City, CA) and SybrGreen Master Mix (Bioline USA Inc., by guest, on O ctober 27, 2015 w w w .jlr.org D ow nloaded from method as previously described (51).



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