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transcription factor staining kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology transcription factor staining kit
    A GPR4 regulates JAK2 and STAT3 phosphorylation via western blotting. n = 3. Scale bar: 100 µm. B Representative IF images of STAT3-Y705 phosphorylation regulated by GPR4. C Quantitative analysis of STAT3-Y705 phosphorylation in GPR4 - OE SW480 cell line and ( D ) GPR4 - KD HCT116 cell lline. Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. E Static treatment reduces both pSTAT3-Y705 and the expression of LOXL2, COL1A1, and TGF-β in GPR4 - OE SW480 cells. F RT-PCR analysis for LOXL2 in SW480 cell lines after receiving Stattic treatment. Two-tailed t test, n = 3 biologically independent samples. G RT-PCR analysis for TGF-β in SW480 cell lines after receiving Stattic treatment. Data are presented as mean ± SD.Two-tailed t test, n = 3 biologically independent samples. H STAT3 <t>transcription</t> factor binding site. I In SW480 cells, STAT3 binds to the LOXL2 promoter region confirmed via ChIP assays. J STAT3 binds to the TGF-β promoter region confirmed via ChIP assays. K RT-PCR assesses the transcriptional activity of LOXL2 promoter regions and TGF-β promoter region L . Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. ns > 0.05.
    Transcription Factor Staining Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+reverse+transcripton+kit/Foxp3%2FTranscription+Factor+Staining+Kit/pmc12859139-605-1-5
    Average 94 stars, based on 23 article reviews
    transcription factor staining kit - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "GPR4 promotes immune exclusion in colon cancer through LOXL2-mediated extracellular matrix remodeling"

    Article Title: GPR4 promotes immune exclusion in colon cancer through LOXL2-mediated extracellular matrix remodeling

    Journal: Nature Communications

    doi: 10.1038/s41467-025-67967-z

    A GPR4 regulates JAK2 and STAT3 phosphorylation via western blotting. n = 3. Scale bar: 100 µm. B Representative IF images of STAT3-Y705 phosphorylation regulated by GPR4. C Quantitative analysis of STAT3-Y705 phosphorylation in GPR4 - OE SW480 cell line and ( D ) GPR4 - KD HCT116 cell lline. Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. E Static treatment reduces both pSTAT3-Y705 and the expression of LOXL2, COL1A1, and TGF-β in GPR4 - OE SW480 cells. F RT-PCR analysis for LOXL2 in SW480 cell lines after receiving Stattic treatment. Two-tailed t test, n = 3 biologically independent samples. G RT-PCR analysis for TGF-β in SW480 cell lines after receiving Stattic treatment. Data are presented as mean ± SD.Two-tailed t test, n = 3 biologically independent samples. H STAT3 transcription factor binding site. I In SW480 cells, STAT3 binds to the LOXL2 promoter region confirmed via ChIP assays. J STAT3 binds to the TGF-β promoter region confirmed via ChIP assays. K RT-PCR assesses the transcriptional activity of LOXL2 promoter regions and TGF-β promoter region L . Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. ns > 0.05.
    Figure Legend Snippet: A GPR4 regulates JAK2 and STAT3 phosphorylation via western blotting. n = 3. Scale bar: 100 µm. B Representative IF images of STAT3-Y705 phosphorylation regulated by GPR4. C Quantitative analysis of STAT3-Y705 phosphorylation in GPR4 - OE SW480 cell line and ( D ) GPR4 - KD HCT116 cell lline. Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. E Static treatment reduces both pSTAT3-Y705 and the expression of LOXL2, COL1A1, and TGF-β in GPR4 - OE SW480 cells. F RT-PCR analysis for LOXL2 in SW480 cell lines after receiving Stattic treatment. Two-tailed t test, n = 3 biologically independent samples. G RT-PCR analysis for TGF-β in SW480 cell lines after receiving Stattic treatment. Data are presented as mean ± SD.Two-tailed t test, n = 3 biologically independent samples. H STAT3 transcription factor binding site. I In SW480 cells, STAT3 binds to the LOXL2 promoter region confirmed via ChIP assays. J STAT3 binds to the TGF-β promoter region confirmed via ChIP assays. K RT-PCR assesses the transcriptional activity of LOXL2 promoter regions and TGF-β promoter region L . Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. ns > 0.05.

    Techniques Used: Phospho-proteomics, Western Blot, Two Tailed Test, Expressing, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Activity Assay

    Related Articles

    Staining:

    Article Title: Electroacupuncture Ameliorates Cyclophosphamide-Induced Ovarian Impairment in Rats With Diminished Ovarian Reserve and is Associated With Th17/Treg-Related Immune Modulation.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Intracellular Fixation/Permeabilization Buffer Kit (E-CK-A109, Elabscience, China). .. Treg/Th17 populations were detected using the Foxp3/Transcription Factor Staining Kit (E-CK-A108, Elabscience, China) in combination with FOXP3 (12-5773-80, eBioscience, USA), IL-17A (25-7177-80, eBioscience, USA), and CD4 (11-0040-81, eBioscience, USA) monoclonal antibodies. .. Cells were incubated with the antibodies for 30min at 4°C in the dark, washed, and resuspended in PBS for analysis on a NovoCyte flow cytometer (Agilent, USA).

    Article Title: Inverse Vaccine Nano-Mimics: In Situ Antigen-Specific Immune Tolerance Induction for Durable Cardiac Repair Post Myocardial Infarction
    Article Snippet: Cells were then surface-stained with fluorophore-conjugated antirat CD4 (E-AB-F1105C, Elabscience, China) and CD25 (PE-30314, Finetest, China) antibodies. .. After surface staining, cells were fixed and permeabilized with Fixation/Permeabilization Concentrate and Permeabilization Buffer (E-CK-A108, Elabscience, China) according to the manufacturer's instructions and intracellularly stained with an anti-rat FOXP3 antibody (E-ABF1351J, Elabscience, China). .. Data were acquired using a NovoCyte flow cytometer.

    Article Title: GPR4 promotes immune exclusion in colon cancer through LOXL2-mediated extracellular matrix remodeling.
    Article Snippet: Single-cell samples were stained for viability assess by using Zombie Yellow Fixable Viability Kit (Biolegend, 423103) at 4°C in the dark for 15 min. Then samples were stained by commercial antibodies for 20 min in dark, including anti-CD45-violent 450 (Elabscience, E-AB-F1136E), anti-CD3-APC (Elabscience, E-AB-F1013Q), anti-CD4-FITC (Elabscience, E-AB-F1097C), anti-CD8-PerCP (Elabscience, E-AB-F1104J). .. Subsequently, Transcription Factor Staining Kit (Elabscience, E-CK-A108) was applied for cells fixation and Permeabilization as recommended method. .. And samples were stained by anti-KI67-PE (Biolegend, 151209) for 20 min in dark.

    Article Title: GPR4 promotes immune exclusion in colon cancer through LOXL2-mediated extracellular matrix remodeling
    Article Snippet: Single-cell samples were stained for viability assess by using Zombie Yellow Fixable Viability Kit (Biolegend, 423103) at 4 °C in the dark for 15 min. Then samples were stained by commercial antibodies for 20 min in dark, including anti-CD45-violent 450 (Elabscience, E-AB-F1136E), anti-CD3-APC (Elabscience, E-AB-F1013Q), anti-CD4-FITC (Elabscience, E-AB-F1097C), anti-CD8-PerCP (Elabscience, E-AB-F1104J). .. Subsequently, Transcription Factor Staining Kit (Elabscience, E-CK-A108) was applied for cells fixation and Permeabilization as recommended method. .. And samples were stained by anti-KI67-PE (Biolegend, 151209) for 20 min in dark.

    Bioprocessing:

    Article Title: Electroacupuncture Ameliorates Cyclophosphamide-Induced Ovarian Impairment in Rats With Diminished Ovarian Reserve and is Associated With Th17/Treg-Related Immune Modulation.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Intracellular Fixation/Permeabilization Buffer Kit (E-CK-A109, Elabscience, China). .. Treg/Th17 populations were detected using the Foxp3/Transcription Factor Staining Kit (E-CK-A108, Elabscience, China) in combination with FOXP3 (12-5773-80, eBioscience, USA), IL-17A (25-7177-80, eBioscience, USA), and CD4 (11-0040-81, eBioscience, USA) monoclonal antibodies. .. Cells were incubated with the antibodies for 30min at 4°C in the dark, washed, and resuspended in PBS for analysis on a NovoCyte flow cytometer (Agilent, USA).



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