whole transcriptome sequencing (Illumina Inc)

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Illumina Inc whole transcriptome sequencing
Whole Transcriptome Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 12785 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+reverse+transcripton+kit/TruSeq+RNA+Library+Prep/pmc10165247-115-12-20
Average 99 stars, based on 12785 article reviews

whole transcriptome sequencing - by Bioz Stars, 2026-10

99/100 stars

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Sample Prep:

Article Title: Targeting fibroblast activation protein-α to treat renal fibrosis.
Article Snippet: RNA quality was checked using an Agilent 2100 bioanalyzer based on RNA integrity. .. The TruSeq RNA librarie were built using the Illumina TruSeq RNA sample preparation kit v2 (Illumina) according to standard protocols. .. And the sequencing was performed using the Illumina NovaSeq 6000 platform.

Article Title: Host microRNA-31-5p regulates KHDRBS3 expression and modulates viral gene expression during KSHV lytic reactivation.
Article Snippet: All RNA samples were analyzed for RNA integrity using the Agilent 2100 Bioanalyzer. .. For miRNA-seq, small RNA library preparation was performed using Illumina TruSeq small RNA sample preparation protocol and sequenced as 50-nucleotide (nt) single-end runs on the HiSeq 2500 platform. .. The remaining pellet (nuclear fraction) was washed with ice-cold HLB buffer and centrifuged at 300 x g at 4◦C for 2 min; this step was repeated three times and then total RNA was isolated from the cytoplasmic and nuclear fractions using mirVana miRNA Isolation Kit (Thermo Fisher Scientific).

Article Title: Genetic Regulation of Monocyte MicroRNAs and Their Implication in Musculoskeletal Diseases: A Cross-Ancestry Expression Quantitative Trait Loci and Imputation Study.
Article Snippet: .. Small RNA sequencing libraries were prepared using TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). .. The constructed libraries were sequenced using Illumina Hiseq2000/2500 following the manufacturer’s protocol.

Article Title: Engineering of plant-derived P450scc for de novo biosynthesis of pregnenolone
Article Snippet: .. Sequencing libraries were generated using the Illumina TruSeq RNA sample preparation kit (Illumina, USA) following the standard protocol. ..

Generated:

Article Title: Regulation of high-density cell growth by Rgg transcription factors in Streptococcus thermophilus S-3.
Article Snippet: BACKGROUND: Streptococcus thermophilus S-3 is an industrial dairy fermentation species, which can modulate growth and metabolism through quorum sensing (QS).. However, the regulatorymechanisms governing high-density bacterial proliferation remain elusive in S. thermophilus S-3.. This study focuses on Rgg, a quorum sensing-associated transcription factor, examining its regulatory role in high-density growth dynamics.

RNA Library Preparation:

RNA Sequencing:

Sequencing:

Synthesized:

Article Title: Transglutaminase 2 modulates inflammatory angiogenesis via vascular endothelial growth factor receptor 2 pathway in inflammatory bowel disease
Article Snippet: .. RNA samples were extracted by TRIzol reagent (Invitrogen, USA). cDNA libraries were synthesized from RNA samples utilizing the TruSeq RNA Library Prep Kit (Illumina, USA) and then sequenced on an Illumina HiSeq. ..

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