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high capacity cdna transcription kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher high capacity cdna transcription kit
    High Capacity Cdna Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 3550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+reverse+transcripton+kit/SYBR+Green+Fast+Advanced+Cells-to-CT+Kit/pmc04965082-44-16-21
    Average 97 stars, based on 3550 article reviews
    high capacity cdna transcription kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Staining:

    Article Title: Identification of key virus–prokaryote infection pairs that contribute to viral shunt in a freshwater lake
    Article Snippet: .. Slide glasses were prepared using DNA staining methods, [ ] except that the SYBR Gold kit (Thermo Fisher Scientific, Oregon, USA; stock solution diluted 1:400) was used instead of the SYBR Green I kits [ ]. .. Prokaryotic cells and virus-like particles were counted under an epifluorescence microscope (BZ-9000, KEYENCE) at 1 000 × magnification.

    SYBR Green Assay:

    Article Title: Identification of key virus–prokaryote infection pairs that contribute to viral shunt in a freshwater lake
    Article Snippet: .. Slide glasses were prepared using DNA staining methods, [ ] except that the SYBR Gold kit (Thermo Fisher Scientific, Oregon, USA; stock solution diluted 1:400) was used instead of the SYBR Green I kits [ ]. .. Prokaryotic cells and virus-like particles were counted under an epifluorescence microscope (BZ-9000, KEYENCE) at 1 000 × magnification.

    Article Title: Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism
    Article Snippet: Total cell RNA was extracted with miRNeasy Mini Kit according to manufacturer’s instructions (Qiagen). .. Protein-coding gene RT-qPCR ( OCT4 , SOX17 , FOXA2 , AFP , ALB ) was performed to DE, HLC, four randomly chosen iPSC samples (10,100.EURCAs, 11,304.EURCCs, 11,104.EURCAs, 13,701.EURCSs), HepG2 cells, PHHs and Human Liver Total RNA (TLR, Ambion). cDNA was generated with High Capacity cDNA Reverse Transcription kit (Applied Biosystems) and RT-qPCR was performed with Power SYBR Green PCR Master Mix (Life Technologies, Cat. No. 1408470, Austin, TX) and gene‐specific primers as published before . .. 7900HT Quantitative Real time qPCR system (Applied Biosystems) with 384-well block was used for signal detection.

    Article Title: A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes
    Article Snippet: RNA extraction was performed on bacteria pellets after aTC induction using the Trizol Max Bacterial RNA Isolation Kit (Invitrogen 16096040). .. Contaminating DNA was removed using the Turbo DNA-free Kit (Invitrogen AM1907) and RNA was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems 4368814). qPCR was performed using the SYBR Green Master Mix (Applied Biosystems 4367659) on a StepOne Plus Real-Time PCR System (Applied Biosystems) using comparative CT. qPCR primers are listed in Table S1. mRNA levels from different samples were normalized to a house-keeping gene ( rpsL ) and mRNA levels in CRISPRi strains were compared to that of a vector-bearing Lp02(dcas9 ) control strain using the ΔΔCT method to determine fold repression. .. U937 monocytes (ATCC CRL-1593.2) were maintained in DMEM (Mediatech 15-013-CV) + 10% FBS + glutamine at 37 °C.

    Real-time Polymerase Chain Reaction:

    Article Title: Network analysis reveals strain-dependent response to misfolded tau aggregates
    Article Snippet: RNA concentration and quality were determined via Nanodrop 200 Spectrophotometer. .. For real-time quantitative Polymerase Chain Reaction (qPCR), cDNA was prepared using a High Capacity cDNA Reverse Transcription kit (Applied Biosystems). qPCR was performed in QuantStudio 3 using the recommended protocol for FAST SYBR (Applied Biosystems) with the following primers: human specific MAPT forward TTGCTCAGGTCAACTGGTTT, human specific MAPT reverse ACTGAGAACCTGAAGCACCA, mouse Gapdh forward AAGGTGAAGGTCGGAGTCAAC, mouse Gapdh reverse GGGGTCATTGATGGCAACAATA. ..

    Article Title: A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes
    Article Snippet: RNA extraction was performed on bacteria pellets after aTC induction using the Trizol Max Bacterial RNA Isolation Kit (Invitrogen 16096040). .. Contaminating DNA was removed using the Turbo DNA-free Kit (Invitrogen AM1907) and RNA was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems 4368814). qPCR was performed using the SYBR Green Master Mix (Applied Biosystems 4367659) on a StepOne Plus Real-Time PCR System (Applied Biosystems) using comparative CT. qPCR primers are listed in Table S1. mRNA levels from different samples were normalized to a house-keeping gene ( rpsL ) and mRNA levels in CRISPRi strains were compared to that of a vector-bearing Lp02(dcas9 ) control strain using the ΔΔCT method to determine fold repression. .. U937 monocytes (ATCC CRL-1593.2) were maintained in DMEM (Mediatech 15-013-CV) + 10% FBS + glutamine at 37 °C.

    Reverse Transcription:

    Article Title: Network analysis reveals strain-dependent response to misfolded tau aggregates
    Article Snippet: RNA concentration and quality were determined via Nanodrop 200 Spectrophotometer. .. For real-time quantitative Polymerase Chain Reaction (qPCR), cDNA was prepared using a High Capacity cDNA Reverse Transcription kit (Applied Biosystems). qPCR was performed in QuantStudio 3 using the recommended protocol for FAST SYBR (Applied Biosystems) with the following primers: human specific MAPT forward TTGCTCAGGTCAACTGGTTT, human specific MAPT reverse ACTGAGAACCTGAAGCACCA, mouse Gapdh forward AAGGTGAAGGTCGGAGTCAAC, mouse Gapdh reverse GGGGTCATTGATGGCAACAATA. ..

    Article Title: Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism
    Article Snippet: Total cell RNA was extracted with miRNeasy Mini Kit according to manufacturer’s instructions (Qiagen). .. Protein-coding gene RT-qPCR ( OCT4 , SOX17 , FOXA2 , AFP , ALB ) was performed to DE, HLC, four randomly chosen iPSC samples (10,100.EURCAs, 11,304.EURCCs, 11,104.EURCAs, 13,701.EURCSs), HepG2 cells, PHHs and Human Liver Total RNA (TLR, Ambion). cDNA was generated with High Capacity cDNA Reverse Transcription kit (Applied Biosystems) and RT-qPCR was performed with Power SYBR Green PCR Master Mix (Life Technologies, Cat. No. 1408470, Austin, TX) and gene‐specific primers as published before . .. 7900HT Quantitative Real time qPCR system (Applied Biosystems) with 384-well block was used for signal detection.

    Article Title: A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes
    Article Snippet: RNA extraction was performed on bacteria pellets after aTC induction using the Trizol Max Bacterial RNA Isolation Kit (Invitrogen 16096040). .. Contaminating DNA was removed using the Turbo DNA-free Kit (Invitrogen AM1907) and RNA was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems 4368814). qPCR was performed using the SYBR Green Master Mix (Applied Biosystems 4367659) on a StepOne Plus Real-Time PCR System (Applied Biosystems) using comparative CT. qPCR primers are listed in Table S1. mRNA levels from different samples were normalized to a house-keeping gene ( rpsL ) and mRNA levels in CRISPRi strains were compared to that of a vector-bearing Lp02(dcas9 ) control strain using the ΔΔCT method to determine fold repression. .. U937 monocytes (ATCC CRL-1593.2) were maintained in DMEM (Mediatech 15-013-CV) + 10% FBS + glutamine at 37 °C.

    Quantitative RT-PCR:

    Article Title: Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism
    Article Snippet: Total cell RNA was extracted with miRNeasy Mini Kit according to manufacturer’s instructions (Qiagen). .. Protein-coding gene RT-qPCR ( OCT4 , SOX17 , FOXA2 , AFP , ALB ) was performed to DE, HLC, four randomly chosen iPSC samples (10,100.EURCAs, 11,304.EURCCs, 11,104.EURCAs, 13,701.EURCSs), HepG2 cells, PHHs and Human Liver Total RNA (TLR, Ambion). cDNA was generated with High Capacity cDNA Reverse Transcription kit (Applied Biosystems) and RT-qPCR was performed with Power SYBR Green PCR Master Mix (Life Technologies, Cat. No. 1408470, Austin, TX) and gene‐specific primers as published before . .. 7900HT Quantitative Real time qPCR system (Applied Biosystems) with 384-well block was used for signal detection.

    Generated:

    Article Title: Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism
    Article Snippet: Total cell RNA was extracted with miRNeasy Mini Kit according to manufacturer’s instructions (Qiagen). .. Protein-coding gene RT-qPCR ( OCT4 , SOX17 , FOXA2 , AFP , ALB ) was performed to DE, HLC, four randomly chosen iPSC samples (10,100.EURCAs, 11,304.EURCCs, 11,104.EURCAs, 13,701.EURCSs), HepG2 cells, PHHs and Human Liver Total RNA (TLR, Ambion). cDNA was generated with High Capacity cDNA Reverse Transcription kit (Applied Biosystems) and RT-qPCR was performed with Power SYBR Green PCR Master Mix (Life Technologies, Cat. No. 1408470, Austin, TX) and gene‐specific primers as published before . .. 7900HT Quantitative Real time qPCR system (Applied Biosystems) with 384-well block was used for signal detection.

    Polymerase Chain Reaction:

    Article Title: Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism
    Article Snippet: Total cell RNA was extracted with miRNeasy Mini Kit according to manufacturer’s instructions (Qiagen). .. Protein-coding gene RT-qPCR ( OCT4 , SOX17 , FOXA2 , AFP , ALB ) was performed to DE, HLC, four randomly chosen iPSC samples (10,100.EURCAs, 11,304.EURCCs, 11,104.EURCAs, 13,701.EURCSs), HepG2 cells, PHHs and Human Liver Total RNA (TLR, Ambion). cDNA was generated with High Capacity cDNA Reverse Transcription kit (Applied Biosystems) and RT-qPCR was performed with Power SYBR Green PCR Master Mix (Life Technologies, Cat. No. 1408470, Austin, TX) and gene‐specific primers as published before . .. 7900HT Quantitative Real time qPCR system (Applied Biosystems) with 384-well block was used for signal detection.

    other:

    Article Title: Circulating miR-340-5p and miR-506-3p as Two Osteo-miRNAs for Predicting Osteoporosis in a Cohort of Postmenopausal Women
    Article Snippet: The PCR amplification of candidate miRNAs was carried out by the ABI 7300 machine (Applied Biosystems, USA) with the aid of the SYBR Green I Master Mix kit (Invitrogen, USA), with results presented by the 2 −ΔΔC method.

    Article Title: Targeting chemoresistant senescent pancreatic cancer cells improves conventional treatment efficacy
    Article Snippet: Synthesis of cDNA was performed using a Maxima First cDNA Synthesis Kit (ThermoFisher Scientific). cDNA was used as a template for qPCR run, and mixed with primers (200 nM) for the gene of interest and SYBRTM Green PCR Master Mix (ThermoFisher Scientific). qPCR analyses were carried out with the FX96 Thermocycler (Biorad, Hercules, USA).

    Article Title: 6α-hydroxylated bile acids mediate TGR5 signalling to improve glucose metabolism upon dietary fiber supplementation in mice
    Article Snippet: Liver, ileum and proximal colon tissues were ground into powders and 30–60 mg of tissues were used for RNA extraction using RNeasy Kit from Qiagen. cDNA synthesis was performed on 1 μg of RNA using High-Capacity cDNA Reverse Transcriptase kit (Applied biosystems). qPCR analyses were performed using the SYBR Green technology (Bio-Rad) and primer sequences are listed in .

    Article Title: Allele-specific silencing as therapy for familial amyotrophic lateral sclerosis caused by the p.G376D TARDBP mutation
    Article Snippet: The polymerase chain reaction (PCR) reaction was prepared with SYBRTM Green PCR Master Mix (Applied Biosystem, Watham, MA, USA) and run in C1000 Touch Thermal Cycler (Bio-Rad), using the following thermal protocol: 94°C 5 min, 40 cycles of 94°C 30 s, 60°C 30 s, 72°C.

    Control:

    Article Title: A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes
    Article Snippet: RNA extraction was performed on bacteria pellets after aTC induction using the Trizol Max Bacterial RNA Isolation Kit (Invitrogen 16096040). .. Contaminating DNA was removed using the Turbo DNA-free Kit (Invitrogen AM1907) and RNA was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems 4368814). qPCR was performed using the SYBR Green Master Mix (Applied Biosystems 4367659) on a StepOne Plus Real-Time PCR System (Applied Biosystems) using comparative CT. qPCR primers are listed in Table S1. mRNA levels from different samples were normalized to a house-keeping gene ( rpsL ) and mRNA levels in CRISPRi strains were compared to that of a vector-bearing Lp02(dcas9 ) control strain using the ΔΔCT method to determine fold repression. .. U937 monocytes (ATCC CRL-1593.2) were maintained in DMEM (Mediatech 15-013-CV) + 10% FBS + glutamine at 37 °C.



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