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Promega superscript reverse transcriptase
Superscript Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+reverse+transcriptase/superscript+reverse+transcriptase/us09833496-887-11-14
Average 90 stars, based on 1 article reviews
superscript reverse transcriptase - by Bioz Stars, 2026-09
90/100 stars

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Article Title: From insect endosymbiont to phloem colonizer: comparative genomics unveils the lifestyle transition of phytopathogenic Arsenophonus strains.
Article Snippet: Complementary DNA (cDNA) was synthetized for 1 μg of each RNA sample using random hexamers (Invitrogen) in combination with the Superscript Month XXXX Volume 0 Issue 0 10.1128/msystems.01496-2415 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m sy st em s on 1 0 A pr il 20 25 b y 2a 09 :b ac 1: 34 a0 :1 8: :3 c1 :4 8. reverse transcriptase (Promega). qPCR assays were conducted as previously described (14), using primers and probes listed in Table S1.

Reverse Transcription:

Article Title: Hyperoside Suppresses Renal Inflammation by Regulating Macrophage Polarization in Mice With Type 2 Diabetes Mellitus.
Article Snippet: Total RNA was isolated from kidney tissues or HG-induced macrophages in vitro using Trizol reagent (Qiagen, San Diego, CA, USA) following the manufacturer’s instructions. .. 1ug RNA was reversely transcribed to cDNA using Oligo(dT)15 primer and superscript reverse transcriptase (Promega Corporation, Madison, WI). .. Target gene expression was quantified by realtime PCR using SYBR Green Supermix and the ABI Real-Time PCR Reaction System (Bio-Rad Laboratories).

Article Title: The roots of Atractylodes japonica Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells
Article Snippet: Total RNA from the cells was isolated with TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. Total RNA was reverse transcribed for 1 h at 42°C in a reaction mixture containing RNA, 1× reverse transcriptase buffer (Promega, Madison, WI), 0.5 mM of dNTP (deoxynucleotide triphosphate), 3 mM MgCl 2 , 5 U RNase inhibitor (Amersham, Piscataway, NJ), 0.5 μM oligo-dT primer, and 5 U of Superscript Reverse Transcriptase (Promega, Madison, WI) in a total volume of 20 μL. ..

Article Title: Identification of microRNAs related with neural germ layer lineage-specific progenitors during reprogramming.
Article Snippet: Differentiated cells can be reprogrammed to embryonic stem cell-like cells called induced pluripotent stem cells (iPSCs), in which the natural developmental differentiation process is reversed.. It is unclear whether the multi-lineage cells can be isolated and identified during reprogramming.. In the current study, we detected the expression of lineage markers, isolated neural lineages, and identified the related microRNAs during iPSC formation.

Article Title: Sargahydroquinoic Acid, a Cyclooxygenase-2 Inhibitor, Attenuates Inflammatory Responses by Regulating NF-κB Inactivation and Nrf2 Activation in Lipopolysaccharide-Stimulated Cells.
Article Snippet: Sargahydroquinoic acid (SHQA) is a major plastoquinone in Sargassum macrocarpum and has shown the capacity to prevent inflammation and oxidative stress.. However, the protective mechanisms were unclear.. The molecular mechanisms of SHQA on ameliorating inflammation and oxidative stress have been investigated, using lipopolysaccharide (LPS)-stimulated macrophages.

Article Title: Hyperoside Suppresses Renal Inflammation by Regulating Macrophage Polarization in Mice With Type 2 Diabetes Mellitus
Article Snippet: Total RNA was isolated from kidney tissues or HG-induced macrophages in vitro using Trizol reagent (Qiagen, San Diego, CA, USA) following the manufacturer’s instructions. .. 1ug RNA was reversely transcribed to cDNA using Oligo(dT) 15 primer and superscript reverse transcriptase (Promega Corporation, Madison, WI). .. Target gene expression was quantified by real-time PCR using SYBR Green Supermix and the ABI Real-Time PCR Reaction System (Bio-Rad Laboratories).

Article Title: From insect endosymbiont to phloem colonizer: comparative genomics unveils the lifestyle transition of phytopathogenic Arsenophonus strains
Article Snippet: .. Complementary DNA (cDNA) was synthetized for 1 μg of each RNA sample using random hexamers (Invitrogen) in combination with the Superscript reverse transcriptase (Promega). qPCR assays were conducted as previously described , using primers and probes listed in . ..

Article Title: From insect endosymbiont to phloem colonizer: comparative genomics unveils the lifestyle transition of phytopathogenic Arsenophonus strains
Article Snippet: .. Total RNA was extracted using a 3% CTAB protocol , treated with DNase and quantified using a Qubit fluorometer. cDNA was synthetized for 1 μg of each RNA sample using random hexamers (Invitrogen) in combination with the Superscript reverse transcriptase (Promega). qPCR assays were conducted as previously described , using primers and probes listed in Table S1. ..

Isolation:

Article Title: Identification of microRNAs related with neural germ layer lineage-specific progenitors during reprogramming.
Article Snippet: Differentiated cells can be reprogrammed to embryonic stem cell-like cells called induced pluripotent stem cells (iPSCs), in which the natural developmental differentiation process is reversed.. It is unclear whether the multi-lineage cells can be isolated and identified during reprogramming.. In the current study, we detected the expression of lineage markers, isolated neural lineages, and identified the related microRNAs during iPSC formation.

cDNA Synthesis:

Article Title: Identification of microRNAs related with neural germ layer lineage-specific progenitors during reprogramming.
Article Snippet: Differentiated cells can be reprogrammed to embryonic stem cell-like cells called induced pluripotent stem cells (iPSCs), in which the natural developmental differentiation process is reversed.. It is unclear whether the multi-lineage cells can be isolated and identified during reprogramming.. In the current study, we detected the expression of lineage markers, isolated neural lineages, and identified the related microRNAs during iPSC formation.

Proliferation Assay:

Article Title: Sargahydroquinoic Acid, a Cyclooxygenase-2 Inhibitor, Attenuates Inflammatory Responses by Regulating NF-κB Inactivation and Nrf2 Activation in Lipopolysaccharide-Stimulated Cells.
Article Snippet: Sargahydroquinoic acid (SHQA) is a major plastoquinone in Sargassum macrocarpum and has shown the capacity to prevent inflammation and oxidative stress.. However, the protective mechanisms were unclear.. The molecular mechanisms of SHQA on ameliorating inflammation and oxidative stress have been investigated, using lipopolysaccharide (LPS)-stimulated macrophages.

Luciferase:

Article Title: Sargahydroquinoic Acid, a Cyclooxygenase-2 Inhibitor, Attenuates Inflammatory Responses by Regulating NF-κB Inactivation and Nrf2 Activation in Lipopolysaccharide-Stimulated Cells.
Article Snippet: Sargahydroquinoic acid (SHQA) is a major plastoquinone in Sargassum macrocarpum and has shown the capacity to prevent inflammation and oxidative stress.. However, the protective mechanisms were unclear.. The molecular mechanisms of SHQA on ameliorating inflammation and oxidative stress have been investigated, using lipopolysaccharide (LPS)-stimulated macrophages.

Real-time Polymerase Chain Reaction:

Article Title: From insect endosymbiont to phloem colonizer: comparative genomics unveils the lifestyle transition of phytopathogenic Arsenophonus strains
Article Snippet: .. Complementary DNA (cDNA) was synthetized for 1 μg of each RNA sample using random hexamers (Invitrogen) in combination with the Superscript reverse transcriptase (Promega). qPCR assays were conducted as previously described , using primers and probes listed in . ..

Article Title: From insect endosymbiont to phloem colonizer: comparative genomics unveils the lifestyle transition of phytopathogenic Arsenophonus strains
Article Snippet: .. Total RNA was extracted using a 3% CTAB protocol , treated with DNase and quantified using a Qubit fluorometer. cDNA was synthetized for 1 μg of each RNA sample using random hexamers (Invitrogen) in combination with the Superscript reverse transcriptase (Promega). qPCR assays were conducted as previously described , using primers and probes listed in Table S1. ..



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