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Becton Dickinson superscript ii kit
Superscript Ii Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+kit/superscript+ii+kit++powerscript++reverse/pmc04337460-45-12-17
Average 90 stars, based on 1 article reviews
superscript ii kit - by Bioz Stars, 2026-11
90/100 stars

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Article Title: TCRζchain expression in T cells from patients with CML
Article Snippet: Chronic myeloid leukemia (CML) is a clonal disorder of the stem cells which has impaired cellmediated immune response.. Such immune dysfunction may be due to reduced expression of the T-cell receptor (TCR) f chain, which is an important component in TCR-mediated signal transduction.. In this study, the TCR f expression level was detected using a real-time polymerase chain reaction with SYBR Green I technique and relative quantification method.

Reverse Transcription:

Article Title: The biophysical property of A549 cells transferred by VEGF-D.
Article Snippet: Total RNAwas extracted from the normal lung tissues and cell lines with Trizol reagent according to the manufacturer’s recommendations (Trizol reagent; Invitrogen). .. The RNA was reverse‐transcribed into first single‐strand cDNA using random hexamer primers, reverse transcriptase, and the Superscript II kit (PowerScriptTM Reverse, BD, San Jose, CA, USA) according to the manufacturer’s instructions. ..

Article Title: Generation of diffuse large B cell lymphoma-associated antigen-specific Vα6/Vβ13+T cells by TCR gene transfer
Article Snippet: Total RNA was extracted from the TCR CD3+T cells that were either gene-transduced or not gene-transduced, according to the manufacturer's recommendations (TRIzol reagent; Invitrogen, USA). .. The RNA (2 μg) was reverse-transcribed into first single-strand cDNA using random hexamer primers, reverse transcriptase, and the Superscript II kit (PowerScriptTM Reverse, BD, USA) according to the manufacturer's instructions. .. The RNA (2 μg) was reverse-transcribed into first single-strand cDNA using random hexamer primers, reverse transcriptase, and the Superscript II kit (PowerScript Reverse, BD, USA) according to the manufacturer's instructions.

Article Title: Characteristics of TCRζ , ZAP-70 , and FcɛRIγ Gene Expression in Patients with T- and NK/T-Cell Lymphoma
Article Snippet: All procedures were conducted according to the guidelines of the Medical Ethics Committee of the Health Bureau of the Guangdong Province in China. table ft1 table-wrap mode=article t1 Table 1. caption a4 Characteristics of the Lymphoma and Healthy Control Samples table ft1 table-wrap mode=article t1 Table 2. caption a4 Details of Subtype Information Real time polymerase chain reaction for TCRζ 3′UTR amplification Total RNA was isolated from the PBMC samples using the TRIzol reagent according to the manufacturer's protocol (Invitrogen, Carlsbad, CA). .. First-strand cDNA was synthesized with random hexamers and reverse transcriptase using the Superscript II Kit (PowerScript Reverse; BD, San Jose, CA) according to the manufacturer's instructions. ..

Random Hexamer:

Article Title: The biophysical property of A549 cells transferred by VEGF-D.
Article Snippet: Total RNAwas extracted from the normal lung tissues and cell lines with Trizol reagent according to the manufacturer’s recommendations (Trizol reagent; Invitrogen). .. The RNA was reverse‐transcribed into first single‐strand cDNA using random hexamer primers, reverse transcriptase, and the Superscript II kit (PowerScriptTM Reverse, BD, San Jose, CA, USA) according to the manufacturer’s instructions. ..

Article Title: Generation of diffuse large B cell lymphoma-associated antigen-specific Vα6/Vβ13+T cells by TCR gene transfer
Article Snippet: Total RNA was extracted from the TCR CD3+T cells that were either gene-transduced or not gene-transduced, according to the manufacturer's recommendations (TRIzol reagent; Invitrogen, USA). .. The RNA (2 μg) was reverse-transcribed into first single-strand cDNA using random hexamer primers, reverse transcriptase, and the Superscript II kit (PowerScriptTM Reverse, BD, USA) according to the manufacturer's instructions. .. The RNA (2 μg) was reverse-transcribed into first single-strand cDNA using random hexamer primers, reverse transcriptase, and the Superscript II kit (PowerScript Reverse, BD, USA) according to the manufacturer's instructions.

Synthesized:

Article Title: Characteristics of TCRζ , ZAP-70 , and FcɛRIγ Gene Expression in Patients with T- and NK/T-Cell Lymphoma
Article Snippet: All procedures were conducted according to the guidelines of the Medical Ethics Committee of the Health Bureau of the Guangdong Province in China. table ft1 table-wrap mode=article t1 Table 1. caption a4 Characteristics of the Lymphoma and Healthy Control Samples table ft1 table-wrap mode=article t1 Table 2. caption a4 Details of Subtype Information Real time polymerase chain reaction for TCRζ 3′UTR amplification Total RNA was isolated from the PBMC samples using the TRIzol reagent according to the manufacturer's protocol (Invitrogen, Carlsbad, CA). .. First-strand cDNA was synthesized with random hexamers and reverse transcriptase using the Superscript II Kit (PowerScript Reverse; BD, San Jose, CA) according to the manufacturer's instructions. ..



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