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script reverse transcriptase kit  (Jena Bioscience)


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    Structured Review

    Jena Bioscience script reverse transcriptase kit
    Script Reverse Transcriptase Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/super+script+iii+reverse+transcriptase+kit/SCRIPT+Reverse+Transcriptase+Kit/pmc05748818-61-1-5
    Average 94 stars, based on 25 article reviews
    script reverse transcriptase kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Synthesized:

    Article Title: Interplay of Interleukin-1 β and Curcumin on VEGF Expression in Breast Cancer Cells
    Article Snippet: RNA was extracted from cell cultures using the NucleoSpin RNA kit (#704933, Macherey-Nagel, Düren, Germany) according to the manufacturer’s recommendations. .. cDNA was synthesized using Script Reverse Transcriptase and oligo dT primers from 1 μg of total RNA (# PCR505 and # PM305, Jenabioscience, Jena, Germany). .. Real-time PCR was performed in a 20 μL volume using a Roche LightCycler 1.0 and the LightCycler FastStart DNA Master SYBR Green I reagents (#03003230001, Roche-Life Science, Mannheim, Germany).

    Article Title: Immunohistochemical analysis of YB-1 expression in the developing mouse eye
    Article Snippet: Briefly, tissue was homogenized in lysis buffer (Macherey and Nagel Nucleospin RNA/Protein kit), and RNA and protein was prepared according to the manufacturer’s recommendations. .. RNA was quantified by UV spectroscopy, and cDNA was synthesized from 500 ng using anchored oligo-dT-primer and Script reverse transcriptase, following the manufacturer’s (Jena Bioscience, Jena, Germany) manual. .. PCR was performed using YourTaq Direct-Load PCR Mix (biotechrabbit, Berlin, Germany) in 30 cycles (96°C for 20 s; 60°C for 20 s; 72°C for 1 min, with a final 10 min extension at 72°C) using the following primers (Eurofins MWG Operon, Ebersberg, Germany): fw1: GGAGAAGTGATG-GAGGGTGC; rev1: GGTTTAGGGTTCTCTGGGCG; fw2: AGAACGAGGGATCGGAAAGC; rev2: TGCCATCCTCTC-TAGGCTGT.

    Reverse Transcription:

    Article Title: Interplay of Interleukin-1 β and Curcumin on VEGF Expression in Breast Cancer Cells
    Article Snippet: RNA was extracted from cell cultures using the NucleoSpin RNA kit (#704933, Macherey-Nagel, Düren, Germany) according to the manufacturer’s recommendations. .. cDNA was synthesized using Script Reverse Transcriptase and oligo dT primers from 1 μg of total RNA (# PCR505 and # PM305, Jenabioscience, Jena, Germany). .. Real-time PCR was performed in a 20 μL volume using a Roche LightCycler 1.0 and the LightCycler FastStart DNA Master SYBR Green I reagents (#03003230001, Roche-Life Science, Mannheim, Germany).

    Article Title: Coordinated IFN-γ/TNF Axis Drives Selective Loss of Activated Enteric Glia in Inflammatory Bowel Diseases.
    Article Snippet: 653 Jo urn al Pr -pr oo f 22 AntiTUBB3 Biotinylated 1:100 Mouse 801212 BioLegend, Inc. AntiGFAP Alexa Flour 488 1:400 Mouse 644704 BioLegend, Inc. Anti-CD4 Unconjugated 1:100 Rat BD (553043) BD Biosciences AntipMLKL (S345) Unconjugated 1:400 Rabbit 37333S Cell Signaling Technologies Secondary antibody AntiRabbit Biotinylated 1:500 Goat 111-065-144 Jackson Anti-Rat Biotinylated 1:500 Goat 554014 BD Biosciences AntiRabbit Alexa Flour 647 1:400 Donkey 406414 BioLegend, Inc. Anti-Rat Dylight 550 1:400 Donkey SA5-10027 ThermoFisher Scientific GmbH FACS antibodies AntiGFAP Brilliant Violet 421 1:200 Mouse 644710 BioLegend, Inc. MHC II I-A/E-A FITC & APCCy7 1:2000 Rat 107606 & 107628 BioLegend, Inc. Western blot antibodies PLP1 Unconjugated 1:500 Mouse MCA839G BIO-RAD Jo urn al Pr e-p roo f 23 655 RNA isolation, reverse transcription, and qPCR 656 RNA was obtained from homogenized tissue or FACS sorted EGC with the column-based RNeasy 657 kit (Macherey-Nagel GmbH). .. After quality control via Nanodrop, 200 ng of the RNA was processed 658 into first strand cDNA using the reverse transcriptase kit (Jena Bioscience GmbH) according to 659 manufactures instructions. .. For qPCR, 2 μL of cDNA was used in a 20 μL SYBR Green based PCR 660 reactions in 96-well format, reaction performed in a BioRad PCR CFX Connect OM.

    Article Title: Immunohistochemical analysis of YB-1 expression in the developing mouse eye
    Article Snippet: Briefly, tissue was homogenized in lysis buffer (Macherey and Nagel Nucleospin RNA/Protein kit), and RNA and protein was prepared according to the manufacturer’s recommendations. .. RNA was quantified by UV spectroscopy, and cDNA was synthesized from 500 ng using anchored oligo-dT-primer and Script reverse transcriptase, following the manufacturer’s (Jena Bioscience, Jena, Germany) manual. .. PCR was performed using YourTaq Direct-Load PCR Mix (biotechrabbit, Berlin, Germany) in 30 cycles (96°C for 20 s; 60°C for 20 s; 72°C for 1 min, with a final 10 min extension at 72°C) using the following primers (Eurofins MWG Operon, Ebersberg, Germany): fw1: GGAGAAGTGATG-GAGGGTGC; rev1: GGTTTAGGGTTCTCTGGGCG; fw2: AGAACGAGGGATCGGAAAGC; rev2: TGCCATCCTCTC-TAGGCTGT.

    other:

    Article Title: Disrupting Pathogenicity in Foodborne Staphylococcus aureus : Biofilm Inhibition and Attenuation of Resistance and Virulence by Tunisian Aromatic Plant Essential Oils.
    Article Snippet: RNA quality and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Article Title: Coordinated IFN-γ/TNF Axis Drives Selective Loss of Activated Enteric Glia in Inflammatory Bowel Diseases.
    Article Snippet: After final washing 650 steps in PBS, tissue was stored in PBS containing 0.02% sodium azide (Carl Roth GmbH + Co. 651 KG).

    Article Title: Coordinated Interferon-γ/Tumor Necrosis Factor Axis Drives Selective Loss of Activated Enteric Glia in Inflammatory Bowel Diseases
    Article Snippet: After washing the tissue twice with PBS, 0.025% Triton X (Merck KGaA) and 0.02% Tween20 (Merck KGaA) in PBS was used to permeabilize the tissue.

    QC/EQA/Proficiency:

    Article Title: Coordinated IFN-γ/TNF Axis Drives Selective Loss of Activated Enteric Glia in Inflammatory Bowel Diseases.
    Article Snippet: 653 Jo urn al Pr -pr oo f 22 AntiTUBB3 Biotinylated 1:100 Mouse 801212 BioLegend, Inc. AntiGFAP Alexa Flour 488 1:400 Mouse 644704 BioLegend, Inc. Anti-CD4 Unconjugated 1:100 Rat BD (553043) BD Biosciences AntipMLKL (S345) Unconjugated 1:400 Rabbit 37333S Cell Signaling Technologies Secondary antibody AntiRabbit Biotinylated 1:500 Goat 111-065-144 Jackson Anti-Rat Biotinylated 1:500 Goat 554014 BD Biosciences AntiRabbit Alexa Flour 647 1:400 Donkey 406414 BioLegend, Inc. Anti-Rat Dylight 550 1:400 Donkey SA5-10027 ThermoFisher Scientific GmbH FACS antibodies AntiGFAP Brilliant Violet 421 1:200 Mouse 644710 BioLegend, Inc. MHC II I-A/E-A FITC & APCCy7 1:2000 Rat 107606 & 107628 BioLegend, Inc. Western blot antibodies PLP1 Unconjugated 1:500 Mouse MCA839G BIO-RAD Jo urn al Pr e-p roo f 23 655 RNA isolation, reverse transcription, and qPCR 656 RNA was obtained from homogenized tissue or FACS sorted EGC with the column-based RNeasy 657 kit (Macherey-Nagel GmbH). .. After quality control via Nanodrop, 200 ng of the RNA was processed 658 into first strand cDNA using the reverse transcriptase kit (Jena Bioscience GmbH) according to 659 manufactures instructions. .. For qPCR, 2 μL of cDNA was used in a 20 μL SYBR Green based PCR 660 reactions in 96-well format, reaction performed in a BioRad PCR CFX Connect OM.

    Spectroscopy:

    Article Title: Immunohistochemical analysis of YB-1 expression in the developing mouse eye
    Article Snippet: Briefly, tissue was homogenized in lysis buffer (Macherey and Nagel Nucleospin RNA/Protein kit), and RNA and protein was prepared according to the manufacturer’s recommendations. .. RNA was quantified by UV spectroscopy, and cDNA was synthesized from 500 ng using anchored oligo-dT-primer and Script reverse transcriptase, following the manufacturer’s (Jena Bioscience, Jena, Germany) manual. .. PCR was performed using YourTaq Direct-Load PCR Mix (biotechrabbit, Berlin, Germany) in 30 cycles (96°C for 20 s; 60°C for 20 s; 72°C for 1 min, with a final 10 min extension at 72°C) using the following primers (Eurofins MWG Operon, Ebersberg, Germany): fw1: GGAGAAGTGATG-GAGGGTGC; rev1: GGTTTAGGGTTCTCTGGGCG; fw2: AGAACGAGGGATCGGAAAGC; rev2: TGCCATCCTCTC-TAGGCTGT.



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