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high stringency wash buffer  (Zymo Research)


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    Structured Review

    Zymo Research high stringency wash buffer
    High Stringency Wash Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stringency+wash+buffer/M-Wash+Buffer/pm40185099-433-38-74
    Average 94 stars, based on 59 article reviews
    high stringency wash buffer - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Purification:

    Article Title: An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation.
    Article Snippet: 50 mL streptavidin C1 magnetic beads (Thermo Fisher Scientific) were added and reactions were incubated at 37 C for 45 minutes to capture the complexes. .. The beads were washed three times with 250 mL of Low Stringency Wash Buffer (13 SSPE, 0.1% SDS, 1% Molecular Cell 85, 1–18.e1–e10, April 17, 2025 e9 NP-40, 4 M urea) and three times with 250 mL of High Stringency Wash Buffer (0.13 SSPE, 0.1% SDS, 1% NP-40, 4 M urea), each wash for 5 minutes at 60 C. RNA was eluted using RNase H, purified using TRIzol and RNA Clean & Concentrator-5 columns (Zymo Research), and then subjected to qRT-PCR as described above. ..

    Quantitative RT-PCR:

    Article Title: An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation.
    Article Snippet: 50 mL streptavidin C1 magnetic beads (Thermo Fisher Scientific) were added and reactions were incubated at 37 C for 45 minutes to capture the complexes. .. The beads were washed three times with 250 mL of Low Stringency Wash Buffer (13 SSPE, 0.1% SDS, 1% Molecular Cell 85, 1–18.e1–e10, April 17, 2025 e9 NP-40, 4 M urea) and three times with 250 mL of High Stringency Wash Buffer (0.13 SSPE, 0.1% SDS, 1% NP-40, 4 M urea), each wash for 5 minutes at 60 C. RNA was eluted using RNase H, purified using TRIzol and RNA Clean & Concentrator-5 columns (Zymo Research), and then subjected to qRT-PCR as described above. ..

    Centrifugation:

    Article Title: Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA
    Article Snippet: The DNA bound column was washed once with 100 μL Zymo M-Washing buffer (Zymo Research), then 200 μL of M-Desulphonation buffer (Zymo Research) was added and allowed to sit on the column for 15 minutes at room temperature. .. After centrifugation, a total of four washing steps were completed using 200 μL of Zymo M-Wash buffer each time. ..

    Article Title: Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA
    Article Snippet: The samples were then pooled together and purified with Cytiva magnetic beads with a 1.0x beads ratio following the recommended protocol. .. After centrifugation, a total of four washing steps were completed using 200 μL of Zymo M-Wash buffer each time. ..

    other:

    Article Title: Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA
    Article Snippet: The samples were then pooled together and purified with Cytiva magnetic beads with a 1.0x beads ratio following the recommended protocol.



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    Reagents and tools table
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    Reagents and tools table

    Journal: The EMBO Journal

    Article Title: A double-agent microRNA regulates viral cross-kingdom infection in animals and plants

    doi: 10.1038/s44318-025-00405-4

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: North2South TM Hybridization Stringency Wash Buffer (2×) , Thermo Scientific , Cat# 37555.

    Techniques: Virus, Recombinant, FLAG-tag, Sequencing, Labeling, Protease Inhibitor, Reverse Transcription, Membrane, Transfection, Hybridization, Blocking Assay, Caspase-3 Activity Assay, Activity Assay, TUNEL Assay, Apoptosis Assay, Fluorescence, Luciferase, SYBR Green Assay, Expressing, Cloning, Extraction, Transformation Assay, Immunoprecipitation, Software