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basophil stimulation buffer il-3  (BUHLMANN Laboratories)

 
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    BUHLMANN Laboratories basophil stimulation buffer il-3
    Basophil Stimulation Buffer Il 3, supplied by BUHLMANN Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stimulation+buffer/basophil+stimulation+buffer+il+3/pm27907922-53-12-17
    Average 90 stars, based on 1 article reviews
    basophil stimulation buffer il-3 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Sustained effect of grass pollen subcutaneous immunotherapy on suppression of allergen-specific basophil response; a real-life, nonrandomized controlled study.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/all.12581 This article is protected by copyright.. All rights reserved.

    Article Title: Distinct Contributory Factors Determine Basophil-Allergen Sensitivity in Grass Pollen Rhinitis and in Anaphylactic Wasp Venom Allergy.
    Article Snippet: .. Briefly, whole blood (with heparin anticoagulant) or sensitised PBMCs was preincubated in basophil stimulation buffer with IL-3 (Buhlmann Laboratories) containing a final concentration of 0.1 and 1 or 0.01–1 μg/mL of wasp venom [1–3] (Hal Allergie) or 0.01–10 or 100 μg/mL of grass pollen allergen (Aquagen ® SQ, freeze-dried allergen extract of grass pollen mix L299; ALK Abello, Madrid, Spain) or 1.665–333.3 ng/mL of D. pteronyssinus extract (Buhlmann Laboratories) at 37 ° C for 15 min. We also included anti-FcεRI mAb (0.55 μg/mL; Buhlmann Laboratories) and fMLP controls (2 μ M ; Sigma-Aldrich). .. Degranulation was stopped by chilling on ice, after which FITCconjugated anti-CD63 mAb, PE-conjugated anti-CD123 mAb, and PerCP-conjugated anti-HLA-DR mAb (BD Biosciences, San Jose, CA, USA) were added and incubated for 20 min on ice.



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    (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

    Journal: PLOS Biology

    Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode

    doi: 10.1371/journal.pbio.3002838

    Figure Lengend Snippet: (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

    Article Snippet: The cells were resuspended in the stimulation buffer (Cisbio) and incubated with different concentrations of C9 peptide diluted in the stimulation buffer for 30 min at 37°C.

    Techniques: Inhibition, Transfection, Control, Expressing