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STEMCELL Technologies Inc stemdiff neural induction protocol
ALS patient-derived cells are unable to regulate FAO during long-term starvation (A) Schematic of human iPSC differentiation. Refer to E and S3F. (B) Western blot of stem cell and neuronal lineage markers. Quantification shows fold change relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Refer to <xref ref-type=Figure S3 F. (C) Human iPSCs differentiated to neuronal progenitor cells with or without TDP43 mutation were starved for 2, 4, and 6 h. Line graph represents FAO dependency mean ± SEM, ∗ p < 0.05. " width="250" height="auto" />
Stemdiff Neural Induction Protocol, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemdiff+neural+induction+protocol/stemdiff+neural+system/pmc08220302-334-3-12
Average 90 stars, based on 1 article reviews
stemdiff neural induction protocol - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "Stress granules inhibit fatty acid oxidation by modulating mitochondrial permeability"

Article Title: Stress granules inhibit fatty acid oxidation by modulating mitochondrial permeability

Journal: Cell Reports

doi: 10.1016/j.celrep.2021.109237

ALS patient-derived cells are unable to regulate FAO during long-term starvation (A) Schematic of human iPSC differentiation. Refer to E and S3F. (B) Western blot of stem cell and neuronal lineage markers. Quantification shows fold change relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Refer to <xref ref-type=Figure S3 F. (C) Human iPSCs differentiated to neuronal progenitor cells with or without TDP43 mutation were starved for 2, 4, and 6 h. Line graph represents FAO dependency mean ± SEM, ∗ p < 0.05. " title="... S3 F. (C) Human iPSCs differentiated to neuronal progenitor cells with or without TDP43 mutation were starved ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: ALS patient-derived cells are unable to regulate FAO during long-term starvation (A) Schematic of human iPSC differentiation. Refer to E and S3F. (B) Western blot of stem cell and neuronal lineage markers. Quantification shows fold change relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Refer to Figure S3 F. (C) Human iPSCs differentiated to neuronal progenitor cells with or without TDP43 mutation were starved for 2, 4, and 6 h. Line graph represents FAO dependency mean ± SEM, ∗ p < 0.05.

Techniques Used: Derivative Assay, Western Blot, Mutagenesis

Related Articles

Cell Culture:

Article Title: BRG1 establishes the neuroectodermal chromatin landscape to restrict dorsal cell fates
Article Snippet: .. Cells were then cultured according to the STEMCELL Technologies’ instructions for the STEMDiff Dual Neural Induction System for the generation of NPCs using dual SMAD inhibition. ..

Article Title: Morphological profiling in human neural progenitor cells classifies hits in a pilot drug screen for Alzheimer’s disease
Article Snippet: Clones with predicted loss-of-function mutations were tested by immunoblotting with primary antibodies against SORLA (1:4000; 611680, BD Transduction Labs) and GAPDH (1:10 000; MAB374, Merck). .. Colonies of hiPSCs from SORL1 ex31 sub-clones and parental wild-type clones were cultured until 70–80% confluent with no visible differentiation before dissociation and retrieval from suspension by centrifugation at 200 g for 3 min. Neural induction was achieved using the StemDiff Neural Induction Kit (IM, 08581, Stem Cell Technologies) and following the embryoid-body protocol as per manufacturer’s instructions. ..

Inhibition:

Article Title: BRG1 establishes the neuroectodermal chromatin landscape to restrict dorsal cell fates
Article Snippet: .. Cells were then cultured according to the STEMCELL Technologies’ instructions for the STEMDiff Dual Neural Induction System for the generation of NPCs using dual SMAD inhibition. ..

Article Title: CACNA1A loss-of-function affects neurogenesis in human iPSC-derived neural models.
Article Snippet: .. iPSC-derived NPCs were generated by dual inhibition of SMAD signaling using the STEMdiff Neural System (STEMCELL Technologies) according to manufacturer’s instructions. .. Briefly, iPSC colonies were detached by incubation with StemProTM AccutaseTM Cell Dissociation Reagent (A1110501, Thermo Fisher Scientific) for 5 min and cells were collected by pipetting with DMEM/F12 medium (11,330,057, Thermo Fisher Scientific) to make a single cell suspension.

Clone Assay:

Article Title: Morphological profiling in human neural progenitor cells classifies hits in a pilot drug screen for Alzheimer’s disease
Article Snippet: Clones with predicted loss-of-function mutations were tested by immunoblotting with primary antibodies against SORLA (1:4000; 611680, BD Transduction Labs) and GAPDH (1:10 000; MAB374, Merck). .. Colonies of hiPSCs from SORL1 ex31 sub-clones and parental wild-type clones were cultured until 70–80% confluent with no visible differentiation before dissociation and retrieval from suspension by centrifugation at 200 g for 3 min. Neural induction was achieved using the StemDiff Neural Induction Kit (IM, 08581, Stem Cell Technologies) and following the embryoid-body protocol as per manufacturer’s instructions. ..

Suspension:

Article Title: Morphological profiling in human neural progenitor cells classifies hits in a pilot drug screen for Alzheimer’s disease
Article Snippet: Clones with predicted loss-of-function mutations were tested by immunoblotting with primary antibodies against SORLA (1:4000; 611680, BD Transduction Labs) and GAPDH (1:10 000; MAB374, Merck). .. Colonies of hiPSCs from SORL1 ex31 sub-clones and parental wild-type clones were cultured until 70–80% confluent with no visible differentiation before dissociation and retrieval from suspension by centrifugation at 200 g for 3 min. Neural induction was achieved using the StemDiff Neural Induction Kit (IM, 08581, Stem Cell Technologies) and following the embryoid-body protocol as per manufacturer’s instructions. ..

Centrifugation:

Article Title: Morphological profiling in human neural progenitor cells classifies hits in a pilot drug screen for Alzheimer’s disease
Article Snippet: Clones with predicted loss-of-function mutations were tested by immunoblotting with primary antibodies against SORLA (1:4000; 611680, BD Transduction Labs) and GAPDH (1:10 000; MAB374, Merck). .. Colonies of hiPSCs from SORL1 ex31 sub-clones and parental wild-type clones were cultured until 70–80% confluent with no visible differentiation before dissociation and retrieval from suspension by centrifugation at 200 g for 3 min. Neural induction was achieved using the StemDiff Neural Induction Kit (IM, 08581, Stem Cell Technologies) and following the embryoid-body protocol as per manufacturer’s instructions. ..

Generated:

Article Title: CACNA1A loss-of-function affects neurogenesis in human iPSC-derived neural models.
Article Snippet: .. iPSC-derived NPCs were generated by dual inhibition of SMAD signaling using the STEMdiff Neural System (STEMCELL Technologies) according to manufacturer’s instructions. .. Briefly, iPSC colonies were detached by incubation with StemProTM AccutaseTM Cell Dissociation Reagent (A1110501, Thermo Fisher Scientific) for 5 min and cells were collected by pipetting with DMEM/F12 medium (11,330,057, Thermo Fisher Scientific) to make a single cell suspension.

other:

Article Title: Determinants of astrocytic pathology in stem cell models of primary tauopathies.
Article Snippet: Technologies S (2021) Generation and Culture of Neural Progenitor Cells Using the STEMdiffTM Neural System https://cdn.stemcell. com/media/files/manual/10000005588-Generation_Neural_Progenitor_Cells_from_hPSCs_using_STEMdiff_Neural_Induction_Medium. pdf?_ga=2.159611133.1775495983.1675356130-1992370814.1675356129 32.

Article Title: Assessment of The Broad-Spectrum Host Targeting Antiviral Efficacy of Halofuginone Hydrobromide in Human Airway, Intestinal and Brain Organoid Models
Article Snippet: Dorsal forebrain regionalized neural organoids (RNOs) were generated using the STEMDiffTM Dorsal Forebrain Organoid Differentiation Kit (STEMCELL TechnologiesTM) and STEMDiffTM Neural Organoid Maintenance Kit (STEMCELL TechnologiesTM) according to the manufacturer’s protocols with minor modifications.

Produced:

Article Title: Oligonucleotide compositions and methods thereof
Article Snippet: ALS Neurons: Neuronal differentiation of iPSCs: iPSCs derived from fibroblasts from a C9orf72-associated ALS patient (female, 64 years old) were obtained from RUCDR Infinite Biologics. iPSCs were maintained as colonies on Corning Matrigel matrix (Sigma-Aldrich, St. Louis, Mo.) in mTeSR1 medium (STEMCELL Technologies, Vancouver, BC). .. Neural progenitors were produced using the STEMdiff Neural System (STEMCELL Technologies, Vancouver, BC). iPSCs were suspended in an AggreWell800 plate and grown as embryoid bodies in STEMdiff Neural Induction Medium for 5 days, with daily 75% medium changes. ..



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STEMCELL Technologies Inc stemdiff neural induction protocol
ALS patient-derived cells are unable to regulate FAO during long-term starvation (A) Schematic of human iPSC differentiation. Refer to E and S3F. (B) Western blot of stem cell and neuronal lineage markers. Quantification shows fold change relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Refer to <xref ref-type=Figure S3 F. (C) Human iPSCs differentiated to neuronal progenitor cells with or without TDP43 mutation were starved for 2, 4, and 6 h. Line graph represents FAO dependency mean ± SEM, ∗ p < 0.05. " width="250" height="auto" />
Stemdiff Neural Induction Protocol, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemdiff+neural+induction+protocol/stemdiff+neural+system/pmc08220302-334-3-12
Average 90 stars, based on 1 article reviews
stemdiff neural induction protocol - by Bioz Stars, 2026-10
90/100 stars
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ALS patient-derived cells are unable to regulate FAO during long-term starvation (A) Schematic of human iPSC differentiation. Refer to E and S3F. (B) Western blot of stem cell and neuronal lineage markers. Quantification shows fold change relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Refer to <xref ref-type=Figure S3 F. (C) Human iPSCs differentiated to neuronal progenitor cells with or without TDP43 mutation were starved for 2, 4, and 6 h. Line graph represents FAO dependency mean ± SEM, ∗ p < 0.05. " width="100%" height="100%">

Journal: Cell Reports

Article Title: Stress granules inhibit fatty acid oxidation by modulating mitochondrial permeability

doi: 10.1016/j.celrep.2021.109237

Figure Lengend Snippet: ALS patient-derived cells are unable to regulate FAO during long-term starvation (A) Schematic of human iPSC differentiation. Refer to E and S3F. (B) Western blot of stem cell and neuronal lineage markers. Quantification shows fold change relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Refer to Figure S3 F. (C) Human iPSCs differentiated to neuronal progenitor cells with or without TDP43 mutation were starved for 2, 4, and 6 h. Line graph represents FAO dependency mean ± SEM, ∗ p < 0.05.

Article Snippet: Differentiation to neural progenitor cells was done using STEMdiff neural induction protocol (STEM CELL Technologies, with addition of SMADi).

Techniques: Derivative Assay, Western Blot, Mutagenesis